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家蚕胚胎细胞系
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  “家蚕胚胎细胞系”译为未确定词的双语例句
     CHARACTERIZATION OF BM-21E-HNU5, A CELL LINE FROM EMBRYOS OF BOMBYX MORI
     家蚕胚胎细胞系BM-21E-HNU5的生物学特征研究
短句来源
     Establishment and Characterization of Embryo Cell Line of Bombyx mori-SWU1
     家蚕胚胎细胞系BmE-SWU1的建立及其生物学特性
短句来源
     Establishment and characterization of an embryo cell line of Bombyx mori,BmE-SWU2
     家蚕胚胎细胞系BmE-SWU2的建立及其生物学特性研究
短句来源
     Studies on Isoenzymes of BmE-SWU2 Cell Line Derived From Silkworm Embryo
     家蚕胚胎细胞系(BmE-SWU2)同工酶的研究
短句来源
     The Establishment of an Embryonic Cell Line SWAU2-BmE of Silkworm and Study on RAPD and SSR Thereof
     家蚕胚胎细胞系SWAU2-BmE的建立及其RAPD和SSR的研究
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  相似匹配句对
     Studies on Isoenzymes of BmE-SWU2 Cell Line Derived From Silkworm Embryo
     家蚕胚胎细胞(BmE-SWU2)同工酶的研究
短句来源
     Establishment and Characterization of Embryo Cell Line of Bombyx mori-SWU1
     家蚕胚胎细胞BmE-SWU1的建立及其生物学特性
短句来源
     Method of Establishing Embryonic Stem(ES) Cell line
     胚胎细胞方法
短句来源
     CHARACTERIZATION OF BM-21E-HNU5, A CELL LINE FROM EMBRYOS OF BOMBYX MORI
     家蚕胚胎细胞BM-21E-HNU5的生物学特征研究
短句来源
     Establishment and characterization of an embryo cell line of Bombyx mori,BmE-SWU2
     家蚕胚胎细胞BmE-SWU2的建立及其生物学特性研究
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Established cell line Bm-21E-HNUS,from embryos of Bombyx mori,wasinfected with Plutella xylostella granulosis virus (PxGV). Transmmission electronmicroscopic examination of cell cultures in feted showed virogenic stroma and nucleocapsids and enveloped nucleocapsids in nuclei 5 days pi. The vinous replicated escaped the nuclei by budding through the nuclear membrane. Many capsules werefound in some special vacuoles in cytoplasma 6 days pi. There were many miniamvesicles in both the nuclei infected and the vacuoles...

Established cell line Bm-21E-HNUS,from embryos of Bombyx mori,wasinfected with Plutella xylostella granulosis virus (PxGV). Transmmission electronmicroscopic examination of cell cultures in feted showed virogenic stroma and nucleocapsids and enveloped nucleocapsids in nuclei 5 days pi. The vinous replicated escaped the nuclei by budding through the nuclear membrane. Many capsules werefound in some special vacuoles in cytoplasma 6 days pi. There were many miniamvesicles in both the nuclei infected and the vacuoles containing capsules.

用小菜蛾颗粒体病毒[PlutellaxylostellagranulosisVirus(PxGV)]侵染家蚕胚胎细胞系Bm-21E-HNU5,对受染细胞的超薄切片微电镜观察,在攻毒后120h的受染细胞核内开始发现病毒发生基质、核衣壳和病毒粒子;病毒粒子以出芽方式通过细胞核膜排出核外;攻毒后144h蒴状体所在的囊膜和细胞核中可见大量小膜泡.

To be acquainted with the biochemical characters of isoenzymes in BmE-SWU2 cell line and observe the primary expression of the isoenzyme genes,we carried out the experiments on 4 isoenzymes,viz α-eastase(α-EST),β-eastase(β-EST),lactic dehydrogenase(LDH)and malic dehydrogenase(MDH),in BmE-SWU2,Sf21,Hi5,Sf9 and BmN cell lines by active-PAGE.The differences of isoenzymes between BmE-SWU2 and its original embryos were also compared.The results indicated that there were no obvious differences in isoenzymes between...

To be acquainted with the biochemical characters of isoenzymes in BmE-SWU2 cell line and observe the primary expression of the isoenzyme genes,we carried out the experiments on 4 isoenzymes,viz α-eastase(α-EST),β-eastase(β-EST),lactic dehydrogenase(LDH)and malic dehydrogenase(MDH),in BmE-SWU2,Sf21,Hi5,Sf9 and BmN cell lines by active-PAGE.The differences of isoenzymes between BmE-SWU2 and its original embryos were also compared.The results indicated that there were no obvious differences in isoenzymes between the 10~(th) generation.The 25~(th) generation.The isoenzymes expression pattern between BmE-SWU2 and the embryos showed high similarity.

以新建的家蚕胚胎细胞系(BmE-SWU2)细胞为材料,应用非变性聚丙烯酰胺凝胶电泳(Active-PAGE)不连续系统,进行α-酯酶(α-EST)、β-酯酶(β-EST)、乳酸脱氢酶(LDH)和苹果酸脱氢酶(MDH)的同工酶研究,观察了在建系过程中同工酶的表达情况,比较了BmE-SWU2细胞系与其源胚胎的同工酶差异。结果显示,在第10代和第25代之间,尚未有明显的表达差异。该细胞系和源胚胎同工酶的比较发现,二者的同工酶表达谱具有很高的相似性。

An indirect fluorescent-antibody assay(IFA) was established to detect Nosema bombycis with polyclonal antibody prepared against total proteins extracted from germinated N.bombycis.By IFA,antisera against N.bombycis reacted strongly with the mature spores collected from silkworm and with the spores in the cell culture(BmE dell-line),and obvious distinction was observed between the spores and the host cell for their intensity of fluorescent light.The polar tube of N.bombycis was also noticed.These results demonstrated...

An indirect fluorescent-antibody assay(IFA) was established to detect Nosema bombycis with polyclonal antibody prepared against total proteins extracted from germinated N.bombycis.By IFA,antisera against N.bombycis reacted strongly with the mature spores collected from silkworm and with the spores in the cell culture(BmE dell-line),and obvious distinction was observed between the spores and the host cell for their intensity of fluorescent light.The polar tube of N.bombycis was also noticed.These results demonstrated that polyclonal antisera of N.bombycis can be used for indirect immunofluorescence antibody staining to detect N.bombycis and that IFA is a simple,stable and fast method with good specificity.Therefore,IFA can be used in the research fields of the morphology,epidemiology and protein localization of N.bombycis.

通过制备家蚕微孢子虫总蛋白多克隆抗体,建立了多克隆抗体免疫荧光检测方法(IFA),并利用此方法特异性地检测出了体外培养细胞系(家蚕胚胎细胞系BmE-SWU1)中处于不同发育阶段的家蚕微孢子虫。结果在细胞爬片中可观察到呈较强的黄绿色荧光微孢子虫以及在BmE-SWU1细胞中的寄生分布情况,还可看到孢子极丝的弹出等现象。IFA检测方法鉴别诊断家蚕微粒子虫具有快速、特异性强、灵敏度高、稳定性好、操作简便等特点,可以广泛应用于微孢子虫形态学、流行病学以及蛋白质的定位等研究领域。

 
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