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   家蚕胚胎细胞系 在 蚕蜂与野生动物保护 分类中 的翻译结果: 查询用时:0.049秒
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  “家蚕胚胎细胞系”译为未确定词的双语例句
    Establishment and Characterization of Embryo Cell Line of Bombyx mori-SWU1
    家蚕胚胎细胞系BmE-SWU1的建立及其生物学特性
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    Studies on Isoenzymes of BmE-SWU2 Cell Line Derived From Silkworm Embryo
    家蚕胚胎细胞系(BmE-SWU2)同工酶的研究
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    Studies on the Cellular Pathology of DNV-like and the Establishment of an Embryonic Cell Line from Silkworm
    家蚕类浓核病毒的细胞病理学研究及家蚕胚胎细胞系SWAU1-BmE的建立
短句来源
    The similarity index Nei and genetic distance of the 9 samples were calculated and the phylogeny tree of 9 samples was constructed by UPGMA. Results showed that 2 groups were divided,one group including the 3 embryo cell lines and the embryo of XQ has close relative. Another group constructed by five insect cell lines came from different species,their genetic distance was closer than the 3 embryo cell lines.
    测定了9个样品间的Nei's相似系数和遗传距离,构建了系统发育树,结果表明本实验室建立的3个家蚕胚胎细胞系和家蚕“夏芳×秋白”聚为一簇,亲缘关系较近,而来自不同物种的五个传代昆虫细胞系聚为一簇,它们之间的遗传距离比3个家蚕胚胎细胞系之间的遗传距离更小。
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To be acquainted with the biochemical characters of isoenzymes in BmE-SWU2 cell line and observe the primary expression of the isoenzyme genes,we carried out the experiments on 4 isoenzymes,viz α-eastase(α-EST),β-eastase(β-EST),lactic dehydrogenase(LDH)and malic dehydrogenase(MDH),in BmE-SWU2,Sf21,Hi5,Sf9 and BmN cell lines by active-PAGE.The differences of isoenzymes between BmE-SWU2 and its original embryos were also compared.The results indicated that there were no obvious differences in isoenzymes between...

To be acquainted with the biochemical characters of isoenzymes in BmE-SWU2 cell line and observe the primary expression of the isoenzyme genes,we carried out the experiments on 4 isoenzymes,viz α-eastase(α-EST),β-eastase(β-EST),lactic dehydrogenase(LDH)and malic dehydrogenase(MDH),in BmE-SWU2,Sf21,Hi5,Sf9 and BmN cell lines by active-PAGE.The differences of isoenzymes between BmE-SWU2 and its original embryos were also compared.The results indicated that there were no obvious differences in isoenzymes between the 10~(th) generation.The 25~(th) generation.The isoenzymes expression pattern between BmE-SWU2 and the embryos showed high similarity.

以新建的家蚕胚胎细胞系(BmE-SWU2)细胞为材料,应用非变性聚丙烯酰胺凝胶电泳(Active-PAGE)不连续系统,进行α-酯酶(α-EST)、β-酯酶(β-EST)、乳酸脱氢酶(LDH)和苹果酸脱氢酶(MDH)的同工酶研究,观察了在建系过程中同工酶的表达情况,比较了BmE-SWU2细胞系与其源胚胎的同工酶差异。结果显示,在第10代和第25代之间,尚未有明显的表达差异。该细胞系和源胚胎同工酶的比较发现,二者的同工酶表达谱具有很高的相似性。

An indirect fluorescent-antibody assay(IFA) was established to detect Nosema bombycis with polyclonal antibody prepared against total proteins extracted from germinated N.bombycis.By IFA,antisera against N.bombycis reacted strongly with the mature spores collected from silkworm and with the spores in the cell culture(BmE dell-line),and obvious distinction was observed between the spores and the host cell for their intensity of fluorescent light.The polar tube of N.bombycis was also noticed.These results demonstrated...

An indirect fluorescent-antibody assay(IFA) was established to detect Nosema bombycis with polyclonal antibody prepared against total proteins extracted from germinated N.bombycis.By IFA,antisera against N.bombycis reacted strongly with the mature spores collected from silkworm and with the spores in the cell culture(BmE dell-line),and obvious distinction was observed between the spores and the host cell for their intensity of fluorescent light.The polar tube of N.bombycis was also noticed.These results demonstrated that polyclonal antisera of N.bombycis can be used for indirect immunofluorescence antibody staining to detect N.bombycis and that IFA is a simple,stable and fast method with good specificity.Therefore,IFA can be used in the research fields of the morphology,epidemiology and protein localization of N.bombycis.

通过制备家蚕微孢子虫总蛋白多克隆抗体,建立了多克隆抗体免疫荧光检测方法(IFA),并利用此方法特异性地检测出了体外培养细胞系(家蚕胚胎细胞系BmE-SWU1)中处于不同发育阶段的家蚕微孢子虫。结果在细胞爬片中可观察到呈较强的黄绿色荧光微孢子虫以及在BmE-SWU1细胞中的寄生分布情况,还可看到孢子极丝的弹出等现象。IFA检测方法鉴别诊断家蚕微粒子虫具有快速、特异性强、灵敏度高、稳定性好、操作简便等特点,可以广泛应用于微孢子虫形态学、流行病学以及蛋白质的定位等研究领域。

DNA extraction and the polymerase chain reaction (PCR) were used on DNA genomes study of cell lines of Bombyx mori.DNA polymorphic marker analysis was conducted and DNA fingerprint of cell lines of Bombyx mori.was carried out using ISSR and RAPD. Primers that can reliably find polymorphic bands were screened out.26 ISSR primers were selected from them any available,and 797 polymorphic bands were abtained through PCR amplification in 9 samples,including 3 embryo cell lines of Bombyx mori (BmE-SWU1,BmE- SWU2,BmE-SWU3),5...

DNA extraction and the polymerase chain reaction (PCR) were used on DNA genomes study of cell lines of Bombyx mori.DNA polymorphic marker analysis was conducted and DNA fingerprint of cell lines of Bombyx mori.was carried out using ISSR and RAPD. Primers that can reliably find polymorphic bands were screened out.26 ISSR primers were selected from them any available,and 797 polymorphic bands were abtained through PCR amplification in 9 samples,including 3 embryo cell lines of Bombyx mori (BmE-SWU1,BmE- SWU2,BmE-SWU3),5 passage cell lines (BmE,BmN,Sf9,Sf21,Hi5)and the embryos from which BmE-SWU1 originated.The ration of polymorphic bands was 89.9%.43 RAPD primers were selected out through PCR amplification,and 1205 polymorphic bands were obtained in 9 samples. The ration of polymorphic bands was 76.6%.There were many DNA polymorphic bands differences in the cell lines of Bombyx mori.The special DNA markers of the 3 embryo cell lines were found respectively.The similarity index Nei and genetic distance of the 9 samples were calculated and the phylogeny tree of 9 samples was constructed by UPGMA.Results showed that 2 groups were divided,one group including the 3 embryo cell lines and the embryo of XQ has close relative.Another group constructed by five insect cell lines came from different species,their genetic distance was closer than the 3 embryo cell lines.

在建立可靠的家蚕细胞系基因组DNA制备和PCR扩增技术体系的基础上,筛选具有稳定多态性位点的RAPD和ISSR引物,建立家蚕细胞系基因组DNA的ISSR和RAPD分子标记技术体系,检测家蚕细胞系的DNA分子标记多态性,构建细胞系的DNA指纹图谱。筛选出了26个ISSR引物和43个RAPD引物,通过PCR扩增在家蚕胚胎细胞系和传代昆虫细胞系等9个样品中分别获得了797条和1205条多态性条带,多态性达到89.9%和76.6%,不同细胞系的DNA多态性有较大差异,三个家蚕胚胎细胞系具有各自特有的DNA标记。测定了9个样品间的Nei's相似系数和遗传距离,构建了系统发育树,结果表明本实验室建立的3个家蚕胚胎细胞系和家蚕“夏芳×秋白”聚为一簇,亲缘关系较近,而来自不同物种的五个传代昆虫细胞系聚为一簇,它们之间的遗传距离比3个家蚕胚胎细胞系之间的遗传距离更小。

 
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