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    Methods The expression of DcR3 mRNA in PBMCs from 38 patients with SLE(21 active and 17 inactive)and 15 cases of healthy subjects was measured by reverse transcription- polymerase chain reaction (RT- PCR). The correlation was analyzed between DcR3 mRNA expression level and Systemic Lupus Erythematosus Disease Activity Index (SLEDAI).
    方法应用半定量逆转录-聚合酶链反应(RT-PCR)检测38例SLE患者(21例活动期,17例缓解期)和15例正常人PBMCs中DcR3 mRNA的表达水平,分析其与狼疮活动指数(SLEDAI)的相关性。
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    Results The DcR3 mRNA expressed in SLE was significantly higher compared with healthy controls (P<0.01 ),whereas no statistical difference between the active and inactive SLE patients was observed(P>0.05).
    结果SLE患者PBMCs中,DcR3mRNA表达水平明显高于正常人(P<0.01),但疾病不同病期(活动期与缓解期)其表达水平无统计学差异(P>0.05);
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    Results The levels of expression of mRNA of bcl-2 of active SLE patients was significantly higher than that of the quiescent SLE patients,and of the normal healthy donors,and that of quiescent SLE patients was significantly higher than that of control groups.
    结果SLE活动期患者组bcl-2mRNA表达明显高于稳定期患者和正常对照组,稳定期患者和正常对照组比较也有显著性升高;
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    [Results] The percentages of CD1c+ cells (5.4±1.3)% and CD1d+ cells (5.3±1.5)% increased in SLE patients in active stage, with significant difference from those of the controls (P <0.01).
    结果SLE活动组病人CD1c+及CD1d+细胞百分率显著增高(P<0.05),SLE患者血清IFN-r、IL-2水平显著低于正常对照组(P<0.05和P<0.01),IL-4、IL-10水平显著高于正常对照组(P<0.01)。
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    Methods 54 patients with RA(30 active phase and 24 inactive phase),20 patients with other connective tissue diseases other than RA and 20 healthy people were enrolled in the study.
    方法选择正常对照组20例,非类风湿性结缔组织病20例,类风湿性关节炎病人(RA)54例(其中活动期30例,非活动期24例),分别采用免疫散射比浊法测定抗体的浓度;
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    EFFECTS OF DIFFERENT COMPONENTS OF SERUM FROM RADIATION,BURN AND COMBINED RADIATION BURN INJURY ON L TYPE CALCIUM CHANNEL OF CULTURED MYOCARDIAL CELLS
    放射损伤、烧伤与放烧复合伤血清成份对心肌细胞钙离子通道活动的影响
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    Conclusion:db/db diabetes mellitus may induce submandibular gland atrophy and inhibit the proliferation of submandibular gland cells.
    结论:①db/db糖尿病可导致颌下腺组织萎缩及实质细胞形态学改变。 ②PCNA表达逐渐减弱,较对照组明显降低,说明糖尿病可能导致颌下腺腺体增殖活动的减弱。
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    The expression of GITR/GITRL from RA patients was positively associated with the level of DAS28 and ESR(r=0.361,r=0.427,P<0.01].Conclusion:The GITR/GITRL expression of RA patients was higher than that of other groups which may play an important role in the development of RA.
    RA患者GITRmRNA表达水平与DAS28评分及ESR呈明显正相关(r值分别为0.361和0.427,P<0.01)。 结论:RA患者GITR/GITRL表达增多,与RA发病及疾病活动度有关。
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    Methods Fourty-one cases of obese subjects were investigated with Digitrapper electrogastrography(EGG) before and after test meal,32 healthy volunteers with normal body mass index were matched as control.
    方法采用Digitrapper双电极EGG记录仪记录41例单纯性肥胖者餐前和餐后胃肌电活动,以32例正常体重健康志愿者为对照。
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    Objective To detect the ratio of CD4+ and CD8+ T cells and the number of CD4+CD25+ T regulatory cells(Treg)in human peripheral blood mononuclear cells(PBMC)of rheumatoid arthritis(RA)patients and evaluate the significance for diagnosis of RA.
    目的观察活动期类风湿性关节炎(RA)患者外周血T细胞CD4/CD8比值及CD4+ CD25+ T细胞(TR细胞)的数量,探讨其在RA诊治中的价值。
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  active
The Design and Synthesis of a Novel, Orally Active, Selective ETA Antagonist
      
The pharmacokinetics of α- and β-diastereomers of Arteether, a well-known antimalarial drug and its active metabolite dihydroartemisinin (DHA) were studied in Sprague-Dawley rats, Rhesus monkeys and human volunteers.
      
The same compounds were studied using CoMFA for which the molecules were first aligned to a template molecule, which was most active in the series.
      
Among these compounds, 6-benzo[1,3]dioxol-5-yl-4-oxo-hex-5-enoic acid (3) was the most active one.
      
Only 15.33% of the cells was metabolically active at 200 μg/ml methanol extract while 23.57% of the cells was active at 200 μg/ml berberine.
      
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Both cAMP and cGMP which are the important materials regulating the vital functions possess the biological effects which interact and inhibit each other. These effects are similar to the "Yin-Yan" theory in the Traditional Chinese Medicine. For exploring the principle of differentiating symptoms and physical signs based on the Traditional Chinese Medicine in treatment of disease, the content of cAMP in the plasma of patients with deficinncy of vital energy (气虚), deficiency of Yin (阴虚), deficiency of Yan (阳虚),...

Both cAMP and cGMP which are the important materials regulating the vital functions possess the biological effects which interact and inhibit each other. These effects are similar to the "Yin-Yan" theory in the Traditional Chinese Medicine. For exploring the principle of differentiating symptoms and physical signs based on the Traditional Chinese Medicine in treatment of disease, the content of cAMP in the plasma of patients with deficinncy of vital energy (气虚), deficiency of Yin (阴虚), deficiency of Yan (阳虚), deficiency both of vital energy and Yin (气阴双虚), and extreme decease of Yan (亡阳) was assayed. The results of assay have indicated that the content of cAMP in the plasma of patients with deficiency of vital energy, deficiency of Yan and extreme decease of yan is higher than that of normals, but the content of cAMP in plasma of patients with deficiency of Yin and deficiency both of vital energy and Yin is not significantly different from that of normals. It seems that the principle of differentiating symptoms and physical signs based on the Traditional Chinese Medicine in treatment of the disease is related to the content of cAMP in the plasma, although its significance is not yet clear.

cAMP与cGMP是生命活动过程中重要的调节物质,具有相互拮抗,相互制约的生物学效应。这与中医的阴阳学说有相似之处。为了从分子水平来探讨中医理论的物质基础,我们依据八纲辨证方法,选择测定了部分气虚、阴虚、阳虚、气阴双虚及亡阳病人血浆中cAMP含量。结果表明,气虚、阳虚及亡阳病人血浆中cAMP含量均比正常人高;阴虚及气阴双虚病人血浆cAMP含量则与正常人无明显差别。由此可见,中医辨证与血浆cAMP含量有一定关系,但其意义有待进一步研究。

547 sera samples were tested for anti-double strand DNA antibodies with a new method of immunofluorescent assay using blood smears of mice infected with Trypanosoma equiperdum as a substrate.Results showed that all 20 patients with active SLE showed strongly positive reactions; 42 patients with inactive SLE, only 4 showed positive reactions; all other 68 CTD patients except one and all 288 patients with miscellaneous diseases as well as 129 healthy controls showed negative reactions.This method showed rather...

547 sera samples were tested for anti-double strand DNA antibodies with a new method of immunofluorescent assay using blood smears of mice infected with Trypanosoma equiperdum as a substrate.Results showed that all 20 patients with active SLE showed strongly positive reactions; 42 patients with inactive SLE, only 4 showed positive reactions; all other 68 CTD patients except one and all 288 patients with miscellaneous diseases as well as 129 healthy controls showed negative reactions.This method showed rather good correlation with Farr's Assay(82.3%). Authors suggested that this method be applied to detect SLE ( especially active SLE) with high specificity and sensitivity. It was simple, reliable, well repeatable and could be of value in the diagnosis and follow-up of SLE,

用马疫锥虫血片免疫荧光法检测547例血清标本,20树活动期SLE病人全部阳性,42例非活动期或缓解期SLE病人仅4例阳性;其它结缔组织病68例,除1例硬皮症合并SLE外均阴性,288例非结缔组织病和129例健康对照全阴性。将此法同放射免疫(Farr氏)法比较,二者符合率82.3%。作者认为,本法对于活动期系统性红斑性狼疮有高度特异性和敏感性,操作方便,结果可靠,重复性好,可作为诊断SLE的新方法。

According to Farr's ammonia-sulfate technique, we used labelled ~(1(?)5)Ⅰ to measure the binding rate of anti-dsDNA antibodies in normal persons and various kinds of patients. More than 20% of the binding activity was regarded as positive. The positive rates were(63.7%)in SLE patients, 18% in non-SLE connective tissue disease, 12% in other diseases and 7% in the normal persons. A titer of>40% was always found in the patients of active stage with kidney involvement. As compared with the antinuclear antibodies...

According to Farr's ammonia-sulfate technique, we used labelled ~(1(?)5)Ⅰ to measure the binding rate of anti-dsDNA antibodies in normal persons and various kinds of patients. More than 20% of the binding activity was regarded as positive. The positive rates were(63.7%)in SLE patients, 18% in non-SLE connective tissue disease, 12% in other diseases and 7% in the normal persons. A titer of>40% was always found in the patients of active stage with kidney involvement. As compared with the antinuclear antibodies (ANA) test, the immunofluorescence anti-DNA antibodies test was less sensitive but more specific.There was some correlationship between the high anti-DNA antibodies and low C_3 with the disease activity. Therefore, the anti-DNA antibodies test can be used not only as a diagnostic method but also as a monitoring test for the disease activity and kidney involvement in patients with SLE before and after the appropriate treatment.

根据Farr氏的硫酸铵技术,我们采用~(125)I-DNA方法测定了正常人和各种病人的抗双链DNA抗体的结合率。结合活性>20%为阳性。阳性率在SLE病人为63.7%,非SLE结缔组织病为18%,其它疾病12%,正常人仅为7%。有肾脏受累的活动期病人中结合率均>40%。与ANA试验比较放射免疫抗DNA抗体试验敏感性较差,但比较特异。 随着疾病的活动抗DNA抗体升高和补体下降之间有一定关系。因此抗DNA抗体试验不仅可作为一个诊断方法,而且对于SLE有肾脏受累的病人的治疗前后可以作疾病活动性的监护试验。

 
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