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gap启动子
相关语句
  gap promoter
     Constitutive Expression of Human Angiostatin in Pichia pastoris Using the GAP Promoter
     用GAP启动子在毕节酵母中组成型表达人血管抑制素(英文)
短句来源
     A expression vector containing α factor signal pep tide was constructed by inserting modified huIFNα 2b gene into GAP promoter do wnstream of pGAPZ α A, which was transferred into Pichia.
     将克隆修饰的huIFNα 2b基因插入pGAPZα A之GAP启动子下游位点 ,构建成含α factor信号肽的重组表达载体 ,转入毕赤氏酵母 (Pichiapastoris)SMD1168,构建成分泌型表达huIFNα 2b的酵母工程菌株。
短句来源
     After PCR reaction, an expression vector containing α-factor signal peptide was constructed by inserting VAP1 gene into GAP promoter downstream of pGAPZαA. The construct was linearized and introduced into the host SMD1168 genome.
     经PCR扩增,将VAP1基因克隆至穿梭载体pGAPZαA之GAP启动子下游位点,构建成含αfactor信号肽的重组表达载体,获得的重组质粒pGAPZαA VAP1经线性化后转入毕赤氏酵母SMD116 8菌株,构建成分泌型表达VAP1的酵母工程菌株。
短句来源
  “gap启动子”译为未确定词的双语例句
     Methods:The GAP gene promoter was amplified from P.pastoris GS115 and used to replace the AOX1 promoter(pAOX1)on pPIC9K resulting in plasmid pGAP9K.
     方法:应用PCR方法从毕赤酵母染色体中扩增GAP启动子,经测序正确后与已线性化的不含pAOX1启动子的毕赤酵母诱导型表达载体pPIC9K连接,转化大肠杆菌DH5α。
短句来源
     The GAP gene promoter was amplified from P. pastoris GS115 and used to replace the AOX1 promoter (P_(AOX1)) on pPIC9K resulting in plasmid pGAP9K.
     为探索用GAP启动子(P_(GAP))取代AOX1启动子(P_(AOX1)),在毕节酵母(P.pastoris)中组成型表达外源蛋白的可能性,应用PCR 方法从P.
短句来源
     An expression vector, harboring SAM synthetase 2 gene from S.cerevisiae and regulated by the glyceraldehyde-3- phosphate dehydrogenase gene promoter P GAP, was transformed into GS115 strain of P.pastoris.
     将酿酒酵母来源的SAM合成酶 2基因置于GAP启动子调控下 ,构建胞内组成型表达质粒 ,并电转化至毕赤酵母菌株GS115。
短句来源
     The GAP gene promoter was amplified from P.pastoris GS115 and used to replace the AOX1 promoter(P_(AOX1)) on pPIC9K resulting in plasmid pGAP9K.
     为探索用GAP启动子 (PGAP)取代AOX1启动子 (PAOX1) ,在毕节酵母 (P .pastoris)中组成型表达外源蛋白的可能性 ,应用PCR方法从P .
短句来源
     The GAP gene promoter which is about 500 bp was amplified from P.pastoris GS115 and used to replace the AOX1 promoter(pAOX1) on pPIC9K resulting in plasmid pGAP9K.
     应用PCR方法从P. pastoris染色体中扩增了GAP启动子,大小为500bp左右,以其取代诱导型表达载体pPIC9K上的pAOX1,构建了组成型表达载体pGAP9K。
短句来源
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  相似匹配句对
     Historical Development of GAP
     GAP的发展
短句来源
     The upstream sequences of both genes were shown to have promoter activity.
     已经证明两种GAP基因的上游区具有启动子活性。
短句来源
     On Eukaryotic Promoter Prediction
     真核启动子预测
短句来源
     Pulsed measurement of GaP.N photoluminescence
     GaP:N光致发光的脉冲测量
短句来源
     A Brief Account of Promoter Cloning
     启动子克隆概述
短句来源
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  gap promoter
Extracellular expression of hGM-CSF was obtained by cloning its gene in Pichia pastoris under the constitutive glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter with an N-terminal α peptide sequence for its extracellular production.
      
The influence of GAP promoter variants on hirudin production, average plasmid copy number and cell growth in Saccharomyces cerev
      
The above results suggest that GAP promoter is more efficient than AOX1 promoter for the expression of angiostatin in P.
      
Scale-up fermentation of recombinant Candida rugosa lipase expressed in Pichia pastoris using the GAP promoter
      
Extracellular human granulocyte-macrophage colony stimulating factor (hGM-CSF) expression was studied under the control of the GAP promoter in recombinant Pichia pastoris in a series of continuous culture runs (dilution rates from 0.025 to 0.2?h-1).
      
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S-Adenosyl-L-methionine (SAM) is an important metabolic intermediate in the metabolic flux of sulphur. SAM is involved in three key metabolic pathways: transmethylation, transsulfuration and polyamine synthesis. As a potential therapeutic agent, SAM is being used as over the counter drug and nutrient supplement. An expression vector, harboring SAM synthetase 2 gene from S.cerevisiae and regulated by the glyceraldehyde-3- phosphate dehydrogenase gene promoter P GAP, was transformed into GS115 strain of...

S-Adenosyl-L-methionine (SAM) is an important metabolic intermediate in the metabolic flux of sulphur. SAM is involved in three key metabolic pathways: transmethylation, transsulfuration and polyamine synthesis. As a potential therapeutic agent, SAM is being used as over the counter drug and nutrient supplement. An expression vector, harboring SAM synthetase 2 gene from S.cerevisiae and regulated by the glyceraldehyde-3- phosphate dehydrogenase gene promoter P GAP, was transformed into GS115 strain of P.pastoris. Through zeocin resistance and expression screening, a recombinant strain was obtained that had high SAM yield and the fermentation conditions were optimized. The results showed that carbon source, nitrogen source, pH and dissolved oxygen had significant effects on the accumulation of SAM. The SAM production of the recombinant cells reached 2.49 g/L after fermentation for three days under the optimized conditions. The present studies show that fermentation of recombinant P.pastoris strain, expressing heterologous SAM synthetase gene, may be a promising approach for the production of SAM.

S 腺苷甲硫氨酸 (S adenosyl L methionine ,SAM)是生物体硫代谢的重要中间代谢物质 ,在体内起着转甲基、转硫基、转氨丙基的作用 ,具有重要的药用和保健价值。将酿酒酵母来源的SAM合成酶 2基因置于GAP启动子调控下 ,构建胞内组成型表达质粒 ,并电转化至毕赤酵母菌株GS115。经Zeocin抗性和培养筛选到一株高产SAM的重组菌。对重组菌表达工艺的研究表明 ,碳源、氮源、pH和溶解氧对SAM的累积有较大影响。在优化条件下 ,重组细胞培养 3天 ,SAM累积量可达 2 .49g/L。

Growth hormone plays an essential role in the stimulation of somatic growth and development.To produce large amount of grass carp growth hormone (gcGH) of biological activity for further use,this study aimed at high yield expression of gcGH in the yeast Pichia pastoris .The gcGH cDNA was inserted into a yeast vector,pGAPZ α B.Under the control of the promoter GAP (glyceraldehyde 3 phosphate dehydrogenase),a 22 kilodalton protein,similar to the molecular weight of native growth hormone,was detected in...

Growth hormone plays an essential role in the stimulation of somatic growth and development.To produce large amount of grass carp growth hormone (gcGH) of biological activity for further use,this study aimed at high yield expression of gcGH in the yeast Pichia pastoris .The gcGH cDNA was inserted into a yeast vector,pGAPZ α B.Under the control of the promoter GAP (glyceraldehyde 3 phosphate dehydrogenase),a 22 kilodalton protein,similar to the molecular weight of native growth hormone,was detected in the supernatant of transformed yeast.It led to a level as high as of 50 mg/L.This protein could be specifically immunoprecipitated with rabbit polyclonal antibodies raised against gcGH.A receptor ELISA Sandwich method was also perfomed to verify the biological activity of the in vitro expressed gcGH.

为大量获得具有生物学活性的草鱼生长激素 ,对草鱼生长激素cDNA在毕赤酵母中的表达进行了研究。将草鱼生长激素cDNA克隆入毕赤酵母表达载体pGAPZ α B ,构建表达载体pGAPZ α B GH。在三磷酸甘油醛脱氢酶 (GAP)启动子的调控作用下 ,一个类似于天然生长激素大小、分子量约 2 2kD的蛋白获得表达 ,其表达量约 5 0mg L。Western杂交表明 :表达的蛋白与兔抗草鱼生长激素的多克隆抗体特异结合 ,证实该表达蛋白为草鱼生长激素 ;受体夹心式ELISA检测表明 :表达的草鱼生长激素具生物学活性 ,能与不同种鱼来源的肝膜受体特异结合

Pichia. Pastoris SMD1168 strain of high efficie nt and secreting expression for h uIFNα 2b was constructed. A expression vector containing α factor signal pep tide was constructed by inserting modified huIFNα 2b gene into GAP promoter do wnstream of pGAPZ α A, which was transferred into Pichia. Pastoris SMD1168 , for secreting expression huIFNα 2b. The result of SDS PAGE showed that huIFN α 2b possess over 50 percent of total quantity of the secreted proteins with t he expression value of 100~120 μg/ml,...

Pichia. Pastoris SMD1168 strain of high efficie nt and secreting expression for h uIFNα 2b was constructed. A expression vector containing α factor signal pep tide was constructed by inserting modified huIFNα 2b gene into GAP promoter do wnstream of pGAPZ α A, which was transferred into Pichia. Pastoris SMD1168 , for secreting expression huIFNα 2b. The result of SDS PAGE showed that huIFN α 2b possess over 50 percent of total quantity of the secreted proteins with t he expression value of 100~120 μg/ml, the bioactivity was 6.69×10 8IU/m g protein~1.11×10 9IU/mg protein identified by MTT method. huIFNα 2b was purified by column chromatography techniques, and the purification rate reached to 99.7 percent with the recovery rate of 15%~20%, western blot analysis showe d that the expressed huIFNα 2b had the same immunogenicity as natural one.

利用毕赤氏酵母SMD1168构建huIFNα 2b分泌型高效表达工程菌株 ,以提高huIFNα 2b表达量和生物比活性 ,增强临床治疗效果和降低临床应用的毒性副作用。将克隆修饰的huIFNα 2b基因插入pGAPZα A之GAP启动子下游位点 ,构建成含α factor信号肽的重组表达载体 ,转入毕赤氏酵母 (Pichiapastoris)SMD1168,构建成分泌型表达huIFNα 2b的酵母工程菌株。SDS PAGE分析表明 ,该工程菌株huIFNα 2b表达量占菌体分泌总量的 50 %以上 ,表达量为 10 0~ 12 0 μg ml;MTT法测定纯化样品的生物比活性为 6 69× 10 8IU mg蛋白~ 1 11× 10 9IU mg蛋白 ,并通过柱层析分离纯化了huIFNα 2b ,纯度达 99 7% ,回收率达 15%~ 2 0 % ;Westernblotting分析表明表达的huIFNα 2b具有与天然huIFNα 2b相同的免疫原性

 
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