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反义寡核苷酸     
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  antisense oligonucleotide
    Study on the Role of Survivin in Human Clear-cell RCCs and the Effects of Survivin Antisense Oligonucleotide on 786-O Cells and Their Possible Mechanism
    survivin基因在肾透明细胞癌中的表达及survivin反义寡核苷酸对786-O细胞生物学行为的影响
短句来源
    Proliferating cell nuclear antigen antisense oligonucleotide in combined with recombinant adenovirus p53 to treat bladder cancer
    p53重组腺病毒联合增殖细胞核抗原反义寡核苷酸治疗膀胱癌的研究
短句来源
    Malignant Phenotypes Reverse Effects on Prostate Cancer Cells by Antisense Oligonucleotide Targeting Telomerase hTRT Gene
    端粒酶hTRT基因反义寡核苷酸逆转前列腺癌细胞恶性表型的研究
短句来源
    The growth inhibition effects on prostate cancer cells by blocking expression of androgen receptor with antisense oligonucleotide
    反义寡核苷酸阻断雄激素受体表达对前列腺癌细胞生长活性的影响
短句来源
    Study of the effect of lipofectine-induced antisense oligonucleotide of clusterin on bladder cancer EJ cells
    脂质体介导的聚集素反义寡核苷酸对膀胱癌EJ细胞的作用
短句来源
更多       
  antisense oligonucleotides
    Study on BCL-2 Antisense Oligonucleotides Therapy for Bladder Tumor
    BCL-2反义寡核苷酸治疗膀胱肿瘤的研究
短句来源
    After transfection of respiratory tract virus antisense oligonucleotides(ASONs)into PBMCs from SRSNS at the active stage,the gene expression of respiratory tract viruses and the activity of NF-κB were measured through RT-PCR and electro-mobility shift assays,respectively.
    将呼吸道病毒反义寡核苷酸(antisense oligonucleotides,ASONs)转染至SRSNS活动期患儿PBMCs,再检测呼吸道病毒基因表达和NF-κB活性。
短句来源
    Effect of antisense oligonucleotides of bcl-2 on BIU87 cell line of bladder cancer
    bcl-2反义寡核苷酸对膀胱癌BIU87细胞株的影响
短句来源
    Study on antisense oligonucleotides of BCL-2 gene in induction of apoptosis in bladder cancer EJ cell line
    Bcl-2反义寡核苷酸诱导膀胱癌EJ细胞凋亡的研究
短句来源
    Inhibiting effect of vascular endothelial growth factor (VEGF) antisense oligonucleotides on VEGF in bladder carcinoma cells
    VEGF反义寡核苷酸抑制人膀胱癌细胞VEGF表达的作用和效果
短句来源
更多       
  antisense oligomer
    The distribution and stability of fluorescent-labeled PCNA antisense oligomer in human bladder cancer cell BIU-87
    5'-FITC标记的PCNA反义寡核苷酸在人膀胱癌细胞BIU-87中的分布及稳定性分析
短句来源
    Apoptosis induction in prostate cancer cells by HSP70 monoclonal antibody and its antisense oligomer
    HSP70单抗联合反义寡核苷酸诱导前列腺癌细胞凋亡的研究
短句来源
    Effects of HSP70 antisense oligomer on drug resistance of human prostate cancer cells LNCaP and PC-3m
    HSP70反义寡核苷酸逆转LNCaP和PC-3m细胞耐药性的研究
短句来源
    Objective: To study apoptosis induced by HSP70 monoclonal antibody(MAb) and antisense oligomer and its difference between prostatic cancer cell line LNCaP and PC 3m.
    目的:探讨 HSP70单抗及反义寡核苷酸对前列腺癌细胞株 LNCaP和 PC 3m凋亡的诱导作用及差异。
短句来源
    Methods: Apoptosis of LNCaP and PC 3m,which were treated by HSP70 MAb(2μ l), antisense oligomer (10μ mol/L) and HSP70 MAb combining with antisense oligomer for 24, 48, 72, 96 h respectively,were analysed by flow cytometry.
    方法:用流式细胞术分析了 HSP70单抗( 2μ l)、反义寡核苷酸( 10μ mol/L)及单抗联合反义寡核苷酸分别处理 LNCaP和 PC 3m 24、 48、 72、 96 h后,细胞凋亡的发生率。
短句来源
更多       
  anti sense oligonucleotide
    Study on the Role of Survivin in Human Clear-cell RCCs and the Effects of Survivin Antisense Oligonucleotide on 786-O Cells and Their Possible Mechanism
    survivin基因在肾透明细胞癌中的表达及survivin反义寡核苷酸对786-O细胞生物学行为的影响
短句来源
    Proliferating cell nuclear antigen antisense oligonucleotide in combined with recombinant adenovirus p53 to treat bladder cancer
    p53重组腺病毒联合增殖细胞核抗原反义寡核苷酸治疗膀胱癌的研究
短句来源
    Malignant Phenotypes Reverse Effects on Prostate Cancer Cells by Antisense Oligonucleotide Targeting Telomerase hTRT Gene
    端粒酶hTRT基因反义寡核苷酸逆转前列腺癌细胞恶性表型的研究
短句来源
    The growth inhibition effects on prostate cancer cells by blocking expression of androgen receptor with antisense oligonucleotide
    反义寡核苷酸阻断雄激素受体表达对前列腺癌细胞生长活性的影响
短句来源
    Study of the effect of lipofectine-induced antisense oligonucleotide of clusterin on bladder cancer EJ cells
    脂质体介导的聚集素反义寡核苷酸对膀胱癌EJ细胞的作用
短句来源
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      antisense oligonucleotide
    Antiproliferative effects of a c-myc antisense oligonucleotide on human arterial smooth muscle cells
          
    Compared to a control oligonucleotide the antisense oligonucleotide suppressed the proliferation of HSMCs in a concentration-dependent manner without a major cytotoxic effect.
          
    Induction of c-myc expression by serum stimulation of cells was blunted by the antisense oligonucleotide, as shown by immunoblotting.
          
    Tailor-made core-shell nanospheres for antisense oligonucleotide delivery: IV.Adsorption/release behaviour
          
    Objective: To investigate whether the Bcl-2 antisense oligonucleotide (ASODN) may enhance radiation-induced apoptosis in Raji cell line.
          
    更多          
      antisense oligonucleotides
    Antisense oligonucleotides inhibiting ribosomal functions in mycobacteria
          
    We studied the binding of antisense oligonucleotides with 23S rRNA of mycobacteria and E.
          
    Principles of selection of optimal hybridization probes and antisense oligonucleotides are discussed.
          
    Highly Efficient Site-Directed RNA Cleavage by Imidazole-Containing Conjugates of Antisense Oligonucleotides
          
    Furthermore, inhibition of GDH expression by antisense oligonucleotides was toxic to cultured mesencephalic neurons, with dopaminergic neurons being affected at the early stages of this inhibition.
          
    更多          
      antisense oligomer
    When Molt-4 and other tumor cells were treated in vitro with HSP70 antisense oligomer, they displayed propidiumiodide-stained condensed nuclei (intact or fragmented).
          
    The apoptosis induced by HSP antisense oligomer was dose- and time-dependent.
          
    The antisense oligomer induced apoptosis mainly in tumor cells at G1 and S phase, resulting in an inhibition of cell proliferation.
          
    HSP70 antisense oligomer caused DNA-sequence-specific inhibition of HSP70 expression, which preceded apparent apoptosis.
          
    p120 Antisense oligomers inhibited the in vitro proliferation of MIA PaCa-2 cells in a dose-dependent manner, and optimal growth inhibition of greater than 90% was achieved at an antisense oligomer concentration of 100 μmol/L.
          
    更多          
      anti-sense oligonucleotide
    The control groups were treated with anti-sense oligonucleotide (ASODNs) targeting Ki-67 gene.
          
    In contrast, ATmC-1 anti-sense oligonucleotide did not cause a disruption of the myofibrillar organization.
          
    Labeled anti-sense oligonucleotide was used to target the specific mRNA while the protein was targeted with an antibody.
          
    Knocking-out erbB2 with an anti-sense oligonucleotide eliminated heregulin β1-promoted migration and blocked ethanol-induced chemo-migration.
          
    In order to investigate its origin there, we have appliedin situ hybridization in paraffin sections of Bouin's fixed foetal sheep brain, using a short anti-sense oligonucleotide probe.
          
    更多          
      其他


    Objective To investigate the effects of endothelin 1 (ET 1) on mesangial cell proliferation stimulated by thrombin. Methods With cultured human mesangial cells (HMC), the following tests were performed. (1) Stimulative tests: After thrombin stimulation, HMC proliferation was determined by MTT method, concentration of ET 1 in supernatant was measured with radioimmunoassay (RIA) and preproendothelin 1 (ppET 1) mRNA expression of HMC was analysed by Northern blot hybridization. (2) Inhibitory test: The influence...

    Objective To investigate the effects of endothelin 1 (ET 1) on mesangial cell proliferation stimulated by thrombin. Methods With cultured human mesangial cells (HMC), the following tests were performed. (1) Stimulative tests: After thrombin stimulation, HMC proliferation was determined by MTT method, concentration of ET 1 in supernatant was measured with radioimmunoassay (RIA) and preproendothelin 1 (ppET 1) mRNA expression of HMC was analysed by Northern blot hybridization. (2) Inhibitory test: The influence of ET 1 inhibitors on the HMC proliferation following thrombin stimulation at three different levels, i.e. ET 1 antiserum, selective ET receptor type A (ET A) antagonist and antisense oligodeoxynucleotide for ppET 1, was measured by MTT test respetively. Results (1) HMC proliferation was stimulated by thrombin in dose dependent manner and in relation to the time of stimulation. The peak growth level was attained in thrombin concentration of 16 U/ml and incubation time of 16 hours. ET 1 secretion by HMC was increased in parallel with the dose curve and time curve of HMC proliferation; HMC ppET 1 mRNA expression was up regulated by thrombin 16 U/ml with incubation time of 8 hours and 16 hours respectively (7.9 fold, 3.4 fold versus control group). (2) ET 1 inhibitors, ET 1 antiserum, selective ET A receptor antagonist and antisense oligodeoxynucleotide for ppET 1 all significantly inhibit HMC proliferation stimulated by thrombin. Conclusion Thrombin stimulates HMC to produce ET 1 and ET 1 mediates the HMC proliferation.

    目的研究内皮素1(ET1)在凝血酶刺激的肾小球系膜细胞增生中的作用。方法在培养人肾小球系膜细胞(HMC)中进行试验。(1)刺激试验:以凝血酶为刺激物,用氮蓝四唑盐(MTT)方法检测HMC增生程度;用放射免疫分析(RIA)测定ET1蛋白浓度,用Northern杂交分析前内皮素原(ppET1)mRNA表达。(2)抑制试验:将ET1抗血清、选择性内皮素受体ETA拮抗剂和的反义寡核苷酸分别作为三个不同水平的ET1抑制剂,用MTT方法观察它们对凝血酶诱导的HMC增生的抑制作用。结果(1)HMC在凝血酶刺激下增生,呈剂量依赖并与刺激时间有关,当凝血酶16U/ml刺激16小时时HMC增生达高峰(P<0.05);HMC在凝血酶刺激下分泌ET1蛋白增加,其剂量曲线和时间曲线分别与HMC的增生曲线相平行;HMC的ppET1mRNA表达能够被凝血酶上调,凝血酶16U/ml刺激8、16小时其水平分别为对照组的7.9和3.4倍。(2)ET1抗血清、ETA受体拮抗剂和ppET1的反义寡核苷酸均能抑制凝血酶刺激的HMC增生(各组均为P<0.05)。结论凝血酶刺激HMC产生ET1,ET1介导了...

    目的研究内皮素1(ET1)在凝血酶刺激的肾小球系膜细胞增生中的作用。方法在培养人肾小球系膜细胞(HMC)中进行试验。(1)刺激试验:以凝血酶为刺激物,用氮蓝四唑盐(MTT)方法检测HMC增生程度;用放射免疫分析(RIA)测定ET1蛋白浓度,用Northern杂交分析前内皮素原(ppET1)mRNA表达。(2)抑制试验:将ET1抗血清、选择性内皮素受体ETA拮抗剂和的反义寡核苷酸分别作为三个不同水平的ET1抑制剂,用MTT方法观察它们对凝血酶诱导的HMC增生的抑制作用。结果(1)HMC在凝血酶刺激下增生,呈剂量依赖并与刺激时间有关,当凝血酶16U/ml刺激16小时时HMC增生达高峰(P<0.05);HMC在凝血酶刺激下分泌ET1蛋白增加,其剂量曲线和时间曲线分别与HMC的增生曲线相平行;HMC的ppET1mRNA表达能够被凝血酶上调,凝血酶16U/ml刺激8、16小时其水平分别为对照组的7.9和3.4倍。(2)ET1抗血清、ETA受体拮抗剂和ppET1的反义寡核苷酸均能抑制凝血酶刺激的HMC增生(各组均为P<0.05)。结论凝血酶刺激HMC产生ET1,ET1介导了HMC增生。

    Objective Fluorescent-labeled antisense oligomer that is hybridized to 18-bp sequences next to the start coden of human proliferating cell nucleus antigen (PCNA) gene was used to assess the intracellular distribution and stability of the oligonucleotides in the presence or absence of liposome (lipofectin). Method IUM 18-mer Phosphorothioate and unmodified antisense oligonucleotides conjugated with fluorescent 5' -isothiocyananate (5 '-FITC) were cncapsulated by lipofctin or without, and then added into...

    Objective Fluorescent-labeled antisense oligomer that is hybridized to 18-bp sequences next to the start coden of human proliferating cell nucleus antigen (PCNA) gene was used to assess the intracellular distribution and stability of the oligonucleotides in the presence or absence of liposome (lipofectin). Method IUM 18-mer Phosphorothioate and unmodified antisense oligonucleotides conjugated with fluorescent 5' -isothiocyananate (5 '-FITC) were cncapsulated by lipofctin or without, and then added into human bladder cancer cell BIU-87 culture media. The celltular distribution was observed by fluorescence microcopy in fixed cells. Result In the abscnce of lipofectin, the oligonucleotide localized to discrete structures in the cytoplasm of the cells, resulting in a punctate fluorescence pattern . In the presence of lipofectin, cellular fluorescence was markedly increased and the oligonucleotide localized with the nucleus, as well as to discrete stuctures in the cytoplasm. Accumulation of the oligonucleotide in the presence in the presence of lipofectin was time -dependent. Conclusion Cationic lipids can increase the amount of oligonucleotide associated with cells and alter the intracellular distribution of the oligonucleotide .The phosphorotide. The phosphorothioate antisense oligonucleotide remained in the nuclei as well as cytoplasm more stable than unmodified .

    目的观察硫代修饰型及天然型PCNA反义寡核苷酸在脂质体介导下或直接转染人膀胱癌细胞BIU-87后在细胞内的分布及其稳定性。方法将异疏氰酸荧光素(5'-FITC)标记的18mer硫代磷酸化修饰型及未修饰型PCNA反义寡核苷酸在脂质体介导下或直接转染人膀胱癌细胞BIU-87,应用荧光显微镜观察转染细胞从细胞内的时相分布。结果修饰型反义核酸直接转染细胞后30分钟,少数细胞胞浆荧光呈离散型、点状分布,4小时后有少数细胞胞核有强荧光聚积。在脂质体介导下,荧光细胞数目明显增加,4小时后绝大多数细胞迅速积聚于细胞核,4~8小时后,细胞核内荧光强度进一步增强,12小时后细胞荧光减弱甚至消失。而非修饰型反义核酸在直接转染或脂质体介导下均发现荧光在3小时后消失。结论脂质体增加PCNA修饰型反义寡核苷酸与膀胱癌细胞BIU-87结合的数量,同时使其在细胞核内分布聚积明显;PCNA反义寡核苷酸硫代修饰具有更好的稳定性。

    Objective To investigate the effect of endothelin 1 (ET 1) on mesangial cells(MC) proliferation and mesangial matrix expansion in vivo. Methods Antisense oligodeoxynucleotide(As ODN) and its control sequences, sense oligodeoxynucleotide(Se ODN) and mismatch oligodeoxynucleotide(Mis ODN),targeting preproendothelin 1 mRNA (ppET 1) were delivered into the kidney of rats with mesangioproliferative glomerulonephritis (MsPGN) model respectively at day 2 by lipofectin mediated gene transfer method. The efficiency...

    Objective To investigate the effect of endothelin 1 (ET 1) on mesangial cells(MC) proliferation and mesangial matrix expansion in vivo. Methods Antisense oligodeoxynucleotide(As ODN) and its control sequences, sense oligodeoxynucleotide(Se ODN) and mismatch oligodeoxynucleotide(Mis ODN),targeting preproendothelin 1 mRNA (ppET 1) were delivered into the kidney of rats with mesangioproliferative glomerulonephritis (MsPGN) model respectively at day 2 by lipofectin mediated gene transfer method. The efficiency of transfer of ODNs into MC was examined with biotinylated As ODN staining. Four days after the rats were sacrificed, the influence of ODNs on ET 1 production by glomeruli was tested with radioimmunoassay(RIA). The action of ODNs on MC proliferation and mesangial matrix expansion was evaluated with quantitative pathologic analysis. Results ODNs were transferred into MC in vivo. The glomerular ET 1 production was decreased by As ODN [(32±19) ng/g, P <0.05] with reduction of mesangial cell proliferation[(0.82±0.04)/100 μm 2, P <0.05] and mesangial matrix expansion[(12 8±1 6)%, P <0.05], where Se ODN and Mis ODN did not show any effect on all of these. Conclusions Specific inhibition of ET 1 gene expression leads to reduction of proliferation of mesangial cell and expansion of mesangial matrix in rats with MsPGN model. ET 1 is one of the important inflammatory mediators which can stimulate MC proliferation and mesangial matrix expansion in vivo.

    目的研究内皮素1(ET1)在体内系膜细胞和系膜基质增生中的作用。方法应用反义寡核苷酸技术,将前内皮素原的反义寡核苷酸作为ET1基因表达的特异抑制剂,同时设立正义寡核苷酸和错配寡核苷酸作为对照序列(每组3只大鼠)。(1)在大鼠抗Thy11系膜增生性肾小球肾炎模型中,以阳离子脂质体介导的基因转移方法,将各种寡核苷酸链分别导入大鼠肾脏。通过生物素修饰的反义寡核苷酸显色反应,了解寡核苷酸链的体内转移效果。(2)用放射免疫分析方法分析反义、正义,错配寡核苷酸对肾小球ET1蛋白量的影响;用定量病理分析方法观察反义、正义,错配寡核苷酸对系膜细胞和系膜基质增生的作用。结果(1)寡核苷酸链能够进入肾小球系膜细胞。(2)反义寡核苷酸降低了肾小球的ET1蛋白水平[(32±19)ng/g,对照组(126±20)ng/g],抑制了系膜细胞增生[(082±004)个/100μm2]和系膜基质增生(128±16)%,而正义和错配寡核苷酸不影响肾小球的ET1蛋白水平或系膜细胞和系膜基质的病变。结论抑制ET1基因表达能够抑制肾炎大鼠系膜细胞和系膜基质增生,ET1是刺激体...

    目的研究内皮素1(ET1)在体内系膜细胞和系膜基质增生中的作用。方法应用反义寡核苷酸技术,将前内皮素原的反义寡核苷酸作为ET1基因表达的特异抑制剂,同时设立正义寡核苷酸和错配寡核苷酸作为对照序列(每组3只大鼠)。(1)在大鼠抗Thy11系膜增生性肾小球肾炎模型中,以阳离子脂质体介导的基因转移方法,将各种寡核苷酸链分别导入大鼠肾脏。通过生物素修饰的反义寡核苷酸显色反应,了解寡核苷酸链的体内转移效果。(2)用放射免疫分析方法分析反义、正义,错配寡核苷酸对肾小球ET1蛋白量的影响;用定量病理分析方法观察反义、正义,错配寡核苷酸对系膜细胞和系膜基质增生的作用。结果(1)寡核苷酸链能够进入肾小球系膜细胞。(2)反义寡核苷酸降低了肾小球的ET1蛋白水平[(32±19)ng/g,对照组(126±20)ng/g],抑制了系膜细胞增生[(082±004)个/100μm2]和系膜基质增生(128±16)%,而正义和错配寡核苷酸不影响肾小球的ET1蛋白水平或系膜细胞和系膜基质的病变。结论抑制ET1基因表达能够抑制肾炎大鼠系膜细胞和系膜基质增生,ET1是刺激体内系膜细胞和系膜基质增生的重要炎症介质之一

     
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