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酶谱     
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  zymogram
     The hepatic function (ALT, ALB, ALP), myocardial zymogram (AST, LDH, CK, CK-MB), apoptosis rate of PBMC and plasma 8-iso-PGF_(2α) were determined, respectively.
     分别检测肝功能(ALT、ALB、ALP)、心肌酶谱(AST、LDH、CK、CK-MB)、PBMC凋亡率和8-iso-PGF_(2α)水平。
短句来源
     zymogram analysis was used to analyze the activity of MMP-2 and MMP-9. Results (1)In the process of stimulation by TGF-β_1,the type Ⅰ collagen mRNA level of 24 h,48 h and 72 h group was:1.33±0.07,2.46±0.09 and 2.39±0.08 respectivley;
     酶谱分析细胞上清液中MMP-2、MMP-9的活力。 结果(1)TGF-β1刺激HLF-02细胞,244、8、72 h组的Ⅰ型胶原mRNA表达水平分别为1.33±0.07、2.46±0.09、2.39±0.08;
短句来源
     METHOD Myocardial cells of rats infected by CVB3 virus were used to observe the pathological changes and myocardium zymogram changes.
     方法 用CVB3病毒感染大鼠心肌细胞 ,观察正常心肌对照组、病毒感染组、茱萸颗粒给药组心肌细胞病理变化和心肌酶谱变化。
短句来源
     The basic zymogram of peroxidase isoenzymes of Chinese sassafras at the age of 9 years consistes of A1, A2, B, C1, C2 and C3 enzyme bands.
     9年生檫树过氧化物酶同工酶基本酶谱有A_1,A_2,B,C_1,C_2,C_36条酶带;
短句来源
     The progenies of the crosses of Cucumis species with different ploidities [allotetraploid C.hytivus(2n=3x=26,HHCC),allotriploid(2n=3x=26,HCC) and reciprocal F_1 hybirds(2n=2x=19,HC/CH)] and their parental materials were comparatively examined to study the zymogram expressions of POD and EST isoenzymes in different organs.
     以3种不同倍性的Cucum is属种间杂交后代[异源四倍体C.hy tivus(2n=4x=38,HHCC)、异源三倍体(2n=3x=26,HCC)和正反交种间杂种F1(2n=2x=19,HC/CH)]及其双亲为试材,比较研究了过氧化物酶(POD)和酯酶(EST)同工酶在不同器官中的酶谱表达特性.
短句来源
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  enzymes
     PAGE show that the order of thermostability about esterase isozymes is: ESS2>ESS1>EFS2>EFS3>EFF>ESS3 and ESS4>EFS1. The Temperature/Time of inactivation of enzymes: ESS1 and ESS2 are 73℃/4min~76℃/2min;
     二因子五水平回归设计热处理酶样,PAGE分析表明:各酶谱带的热稳定性顾序是:ESS2>ESS1>EFS2>EFS3>EFF>ESS3、ESS4>EFS4;
短句来源
     Methods The renal function (BUN,Cr), myocardial enzymes (AST, LDH, CK, CK-MB, HBDH), serum glucose and Na+ , K+ , Cl- , Ca2+ , Mg2+ were measured in 50 cases of neonatal asphysix and 35 cases controls.
     方法 检测50例窒息新生儿和35例正常对照新生儿的肾功能(BUN,Cr),心肌酶谱(AST,LDH,CK,CK-MB,HBDH),血糖和血清Na~+,K~+,Cl~-,Ca~(2+),Mg~(2+).
短句来源
     Before treatment myocardial enzymes were significantly increased (creatine phosphate kinase, 384~2140 U/L, lactate dehydrogenase, 270~624 U/L, asparate aminotransferase, 40~138 U/L).
     治疗前心肌酶谱显著增高(磷酸肌酸激酶384~2140U/L,乳酸脱氢酶270~624U/L,门冬氨酸转氨酶40~138U/L)。
短句来源
     Monitoring the hemodynamics and myocardial enzymes before operation(T_0),after induction(T_1),after inducing 5min(T_2) and when the operation finished(T_3).
     分别监测术前(T0)、全麻诱导后(T1)、插管后5min(T2)及术毕(T3)的血流动力学和心肌酶谱的变化。
短句来源
     Methods:161 type 2 diabetics with coronary heart disease testified by coronary artery angiography were divided into 4 groups according to fasting plasma glucose level: ≤(6.1)、(6.2)~(7.6)、(7.7)~10 and >10 mmol/L to observe the incidence of myocardial infarction and the level of serum myocardial enzymes.
     方法:经冠状动脉造影证实有冠心病的161例2型糖尿病患者(合并心肌梗死61例),根据其空腹血糖水平分为4组:≤6.1、6.2~7.6、7.7~10.0和>10.0mmol/L,观察心肌梗死的患病率和心肌酶谱的水平。
短句来源
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  enzyme spectrum
     The alteration of myocardial enzyme spectrum was measured by HITACHI76000 Automobile Biochemical Appliance, and the positive result was CK>18.0 u/L, CK-MB>24.0 u/L, AST>38.0 u/L, LDH>50.0 u/L.
     采用日立7600全自动生化分析仪检测心肌酶谱,以CK>184.0 u/L,CK-MB>24.0 u/L,AST>38.0 u/L,LDH>250.0 u/L为阳性。
短句来源
     Method: Myocardial enzyme spectrum (AST, LDH, CK, CK-MB, HBDE), renal function (BUN, Cr), and serum electrolytes (Na+、 K+、 Cl-、 Ca2+、 Mg2+ ) of 40 neonates with moderate and severe asphyxia were detected in acute and recovery phases and compared with normal ones.
     方法:检测40例中、重度窒息新生儿急性期、恢复期的心肌酶谱(AST、LDH、CK、CK-MB、HBDH)、肾功能(BUN、Cr)和血清Na~+、K~+、Cl~-、Ca~(2+)、Mg~(2+),并与30例正常新生儿对照比较。
短句来源
     Myocardial enzyme spectrum results: CK 4301±251u/l,CK MB 2288±328u/l, LDH 2329±216u/l, AST 371±57u/l(n=5)
     心肌酶谱结果 :CK430 1± 2 5 1u/l,CK- MB 2 2 88± 32 8u/l,L DH 2 32 9± 2 16 u/l,AST 371± 5 7u/l(N=5 )。
短句来源
     The alteration of myocardial enzyme spectrum was measured by HITACHI76000 Automobile Biochemical Appliance, and the positive result was CK>[Objective]184.0 μ/L, CK-MB>[Objective]24.0 μ/L, AST>[Objective]38.0 μ/L, LDH>[Objective]250.0 μ/L.
     采用日立7600全自动生化分析仪检测心肌酶谱,以CK>184.0μ/L、CK-MB>24.0μ/L、AST>38.0μ/L、LDH>250.0μ/L为阳性。
短句来源
     Results:The myocardial enzyme spectrum of the patient group was much higher than that in the normal group(P<0.01) and it was positively correlated with plasma TEM concentration(P<0.01 or P<0.05).
     结果:毒鼠强中毒患者与正常年龄组相比心肌酶谱大幅升高(P<0.01),且与毒鼠强浓度呈正相关(P<0.01或P<0.05)。
短句来源
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  enzyme pattern
     A new method for rapidly detecting restriction enzyme pattern of mycobacterium deoxyribonucleic acid (DNA) by capillary electrophoresis with laser induced fluorescence detection (CE-LIFD) was developed.
     建立了毛细管电泳分离 激光诱导荧光检测(CE LIFD)分析分枝杆菌脱氧核糖核酸(DNA)限制性内切酶谱的新方法。
短句来源
     Compared with AGE, CE is more outstanding in resolution and detection time, and it can be applied as a more effective means for DNA restriction enzyme pattern analysis.
     结果表明,CE的分离效能明显高于AGE,是研究DNA限制性内切酶谱的更有效的检测手段。
短句来源
     In order to compare the activities of isozymes in Raji cells infected by simian AIDS type D retrovirus serotype 1 (SRVI ) with that in noninfected cells, the total cell proteins were extracted by ultrasonic vibrator and the enzyme pattern was analyzed with isoelectric focusing in a vertical polyacrylamide gel.
     为了观察猴艾滋病D型逆转录病毒Ⅰ型(SRV1)感染和未感染的Raji细胞同工酶活性的变化,用超声波破碎细胞,提取细胞内总蛋白,然后用垂直薄层聚丙烯酰胺凝胶等电聚焦电泳对其进行酶谱分析。
短句来源
     Restriction Enzyme Pattern Analysis of Mycobacteria DNA by Capillary Electrophoresis with Laser Induced Fluorescence Detection
     毛细管电泳-激光诱导荧光检测分枝杆菌脱氧核糖核酸限制性内切酶谱
短句来源
     The zeatin effect on shoot sheath respiratory rate of spring wheat seedlings differs from varieties, the zeatin can improve the formation of peroxidase isozyme in the sheath and increase the enzyme pattern cord.
     玉米素对不同品种春小麦幼苗的芽鞘呼吸速率的影响是不同的,而且玉米素可以促进春小麦芽鞘的过氧化物酶同工酶的形成,其酶谱带数增加.
短句来源
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  zymogram
The basic enzymatic activity was observed in four zymogram zones.
      
Comparison between varietal purity and zymogram purity in maize
      
The zymogram purity of 5 inbred lines, 7 single cross hybrids and 7 single cross hybrids mixed with their parental lines was examined by a vertical discontinous polyacrylamide gel electrophoresis.
      
It was found that the varietal purity is higher than the zymogram purity and they showed a linear relationship.
      
The regression mefficient (γ) between varietal purity and zymogram purity is 0.898 2.
      
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  enzymes
INHIBITORS OF FILARIAL GAMMA-GLUTAMYL CYCLE ENZYMES AS POSSIBLE MACROFILARICIDAL AGENTS
      
The sensitivity of two γ-glutamyl cycle enzymes i.e.
      
Using the de novo design approach, several trisubstituted thiazole analogs were generated as potential inhibitors of these enzymes.
      
euphratica rapidly activated antioxidant enzymes after the onset of salt stress, which might reduce the accumulation of reactive oxygen species and the subsequent acceleration of lipid peroxide.
      
Thus, the antidotal enzymes and digestive enzymes in the midgut of the larvae were inhibited.
      
更多          
  enzyme spectrum
Nutrition of mother and offspring plays an important role in formation of enzyme spectrum in small intestine of developing organism.
      
The most striking feature of the investigated enzyme spectrum was the 50-2000 times higher specific activity of the aminopeptidase, compared with the other hydrolases.
      
Yeasts and yeast-like fungi produced extracellular hydrolytic enzymes with a potentially positive contribution to the malt enzyme spectrum.
      
The enzyme spectrum of an ectoparasitic mite of the honeybee,Varroa destructor (Anderson and Trueman) was studied usinga semi-quantitative method, especially designed for complex samples which havenot been purified.
      
Improvement of malting quality of barley by complementing the malt enzyme spectrum
      
更多          
  enzyme pattern
Restriction Enzyme Pattern Analysis of Mycobacteria DNA by Capillary Electrophoresis with Laser-Induced Fluorescence Detection
      
Compared with AGE, CE is more outstanding in resolution and detection time, and it can be applied as a more effective means to DNA restriction enzyme pattern analysis.
      
Although the tumours were of different histogenetic origin their enzyme pattern after cultivation was rather uniform.
      
We found a tumor-specific enzyme pattern in both tissues, a finding which is in accord with the results published in the literature.
      
This satellite is highly conserved in restriction enzyme pattern and DNA sequence; sequence heterogeneity is about 10%.
      
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  其他


The aldolase activities and aldolaseisozyme patterns of rat liver duringhepatocarcinogenesis induced by die-thylnitrosamine. (DENA) were investi-gated. The results indicated that thealdolase activity for FIP decreases ra-pidly in liver within the first 8 weeksof DENA administration, then it re-mains at a relatively low level. Thealdolase activity for FDP in liverappears unchanged within the first 16weeks of DENA treatment, but raisesquickly thereafter. Therefore, theFDP/FIP ratio in primary hepatomainduced by...

The aldolase activities and aldolaseisozyme patterns of rat liver duringhepatocarcinogenesis induced by die-thylnitrosamine. (DENA) were investi-gated. The results indicated that thealdolase activity for FIP decreases ra-pidly in liver within the first 8 weeksof DENA administration, then it re-mains at a relatively low level. Thealdolase activity for FDP in liverappears unchanged within the first 16weeks of DENA treatment, but raisesquickly thereafter. Therefore, theFDP/FIP ratio in primary hepatomainduced by DENA, transplantable he-patoma BERH-2 and in normal ratliver are 4.53, 7.52 and 1.03 respectively. The electrophoresis on acetate cel-lulose membrane shows that the de-crease in activity of aldolase B isaccompanied by the appearance ofaldolase A in rat fed DENA for 4 weeksand 8 weeks. Then the activity ofaldolase C appears in rat liver fedDENA for 12 weeks. Among six ratprimary hepatoma we analysed, A_4,A_1C_3 and C_4 bands are present in fourof them, and only A_4 and A_1C_3 bandsin another two. From the results so far obtained,it is suggested that the carcinogen re-presses the aldolase B gene and stimu-lates the aldolase A gene and aldolaseC gene.

二乙基亚硝胺诱发大鼠肝脏癌变时,伴随血清AFP浓度的升高,肝脏组织内醛缩酶活力和醛缩酶同功酶谱发生改变。在诱癌的前8周中,以FIP为底物的醛缩酶活力迅速下降,8周以后保持着较低的活力水平,而以FDP为底物的醛缩酶活力在前16周中变化不显著,在16周后迅速增高。所以对两个底物的活力比,正常成年大鼠肝脏是1.03,原发性肝癌是4.53,移植性肝癌BERH-2第71代是7.52。醋酸纤维薄膜电泳显示正常成年肝脏有醛缩酶B_4,B_3A_1区带,诱癌后B型醛缩酶区带减弱,诱癌4周出现醛缩酶A_4区带,诱癌12周出现A_1C_3区带,部分原发性肝癌中还出现醛缩酶C_4区带。实验结果说明肝癌发生过程中,醛缩酶B基因逐渐受到“封闭”,醛缩酶A基因和醛缩酶C基因依次逐渐“开放”。

A new neutral proteinase-producing strain 0031 has been selected from the air of Hangzhou Leather Plant.In primary observation of the cultural property of the strain 0031,we had found that it was more resemble to Bac. subtilis(A.S.1398),but the enzymogram of fermentative liquor after disc electrophoresis was different from Bac.subtilis (A.S.1398). The adequate cultural conditions for the strain have been established: composition of the liquid medium(%)is:soya bean 3%,corn flocer 2%,wheat bran 3%,KH_2PO_4 0.03%,Na_2HPO_4...

A new neutral proteinase-producing strain 0031 has been selected from the air of Hangzhou Leather Plant.In primary observation of the cultural property of the strain 0031,we had found that it was more resemble to Bac. subtilis(A.S.1398),but the enzymogram of fermentative liquor after disc electrophoresis was different from Bac.subtilis (A.S.1398). The adequate cultural conditions for the strain have been established: composition of the liquid medium(%)is:soya bean 3%,corn flocer 2%,wheat bran 3%,KH_2PO_4 0.03%,Na_2HPO_4 0.4%,pH 8.0(before sterilization).After fermentation in shaking flasks(50ml/500ml/Δ,220 r.p. m.,ecc.dis.2.5cm.), at 28℃,for 38 hrs,the caseiaolytie activity was stablized at about 2000μg/ml, and the maximal activity could reach 2330μg/ml. During the process of study on the properties of enzyme-producing,we have found:1.suitable increase of aeration rate and pH,which will increase the enzymic produce and shorten the fermentative cycle;2.a tendency to slightly repress the enzymic produce and delay the enzymic peak,if fermentative temperature is above 30℃.3.no action of Zn■ and Ca■ on enzymic produce(test concen- tration ZnSO_4 0.005%,CaCl_2 0.3%).

我们从9株水解圈比值(水解圈直径/菌落直径)较大的(一般在7以上)初筛株中,经摇瓶复筛,筛选出一株中性蛋白酶产生菌0031菌株.经初步观察其培养特征较接近于枯草芽孢杆菌(A.S.1398),但其发酵液在盘状电泳上的酶谱,有别于枯草芽孢杆菌,其产酶的摇瓶发酵培养基为:豆饼粉3%,玉米粉2%,麸皮3%,KH_2PO_40.03%,Na_2HPO_40.4%,pH 8.0(消毒前).在50 ml(每500 ml 三角烧瓶)的装量,旋转式摇床(转速220次/分偏距2.5cm)温度28℃的条件下发酵,经34—38小时,酶效价稳定在2000单位左右(测定反应温度30℃)最高效价可达2330单位.在该菌株的产酶特性的探索过程中,我们发现1.适当的加大通气量和提高 pH 能提高产酶量和缩短发酵周期,但美中不足的是酶高峰期较短促,较不利于在发酵过程中控制产酶高峰点.2.提高发酵温度(30℃以上)略有抑制产酶的趋势或有明显的酶高峰期推迟的现象.3.Zn~艹和 Ca~艹(试验浓度分别为 ZnSO_40.005%和 CaCl_2 0.3%)对产酶无促进作用.根据目前所表现的生产性能,0031筛选株是理想的,值得联系实际目...

我们从9株水解圈比值(水解圈直径/菌落直径)较大的(一般在7以上)初筛株中,经摇瓶复筛,筛选出一株中性蛋白酶产生菌0031菌株.经初步观察其培养特征较接近于枯草芽孢杆菌(A.S.1398),但其发酵液在盘状电泳上的酶谱,有别于枯草芽孢杆菌,其产酶的摇瓶发酵培养基为:豆饼粉3%,玉米粉2%,麸皮3%,KH_2PO_40.03%,Na_2HPO_40.4%,pH 8.0(消毒前).在50 ml(每500 ml 三角烧瓶)的装量,旋转式摇床(转速220次/分偏距2.5cm)温度28℃的条件下发酵,经34—38小时,酶效价稳定在2000单位左右(测定反应温度30℃)最高效价可达2330单位.在该菌株的产酶特性的探索过程中,我们发现1.适当的加大通气量和提高 pH 能提高产酶量和缩短发酵周期,但美中不足的是酶高峰期较短促,较不利于在发酵过程中控制产酶高峰点.2.提高发酵温度(30℃以上)略有抑制产酶的趋势或有明显的酶高峰期推迟的现象.3.Zn~艹和 Ca~艹(试验浓度分别为 ZnSO_40.005%和 CaCl_2 0.3%)对产酶无促进作用.根据目前所表现的生产性能,0031筛选株是理想的,值得联系实际目的,进一步研究提高其产酶能力.

It was found that by using an H-medium as a basis medium with suitable amount of 2,4-D (2-3 mg/1. ) and BA (0.5mg/l), better effects were obtained in inducing cellus formations of poplar shoot apices.

采用H培养基附加适量的2,4—D(2—3毫克/升)及BA(0.5毫克/升),诱导杨树茎尖愈伤组织的效果较好。 在低浓度BA(0.1毫克/升)及NAA(0.1毫克/升)的分化培养基上,茎叶很快分化。在愈伤组织形成幼小植株的过程中,同工酶酶谱有明显的变化。

 
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