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酶谱
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  zymogram
    STUDIES ON ALLELIC ZYMOGRAM OF PERIODIC BRUGIA MALAYI FROM ZHEJIANG PROVINCE 
    浙江省周期型马来丝虫等位基因酶谱研究
短句来源
    zymogram analysis was used to analyze the activity of MMP-2 and MMP-9. Results (1)In the process of stimulation by TGF-β_1,the type Ⅰ collagen mRNA level of 24 h,48 h and 72 h group was:1.33±0.07,2.46±0.09 and 2.39±0.08 respectivley;
    酶谱分析细胞上清液中MMP-2、MMP-9的活力。 结果(1)TGF-β1刺激HLF-02细胞,244、8、72 h组的Ⅰ型胶原mRNA表达水平分别为1.33±0.07、2.46±0.09、2.39±0.08;
短句来源
    Esterase isozyme from nine species of the family Oedipodidae,Gomphoceridae,Catantopidea and Acrididae by discontinuous polyacylamide gel electrophoresis method analysied. The results show that the EST isozyme accurately reflects the specificities of different categories. Differences of zymogram between higher categories are larger than those of lower categories,and there are distingishable differences between genuses of same family.
    对棒角蝗科、斑腿蝗科、剑角蝗科和斑翅蝗科9种蝗虫进行了酯酶同工酶分析.通过生化特征,探讨较高级分类阶元之间的差异,研究结果表明,9种蝗虫共显现26条酶带,种间没有出现共同酶带,不同科样品之间酶谱差异很大,显而易见,同属不同种间的相似性大于同科不同属种间的相似程度,后者又明显大于不同科种间的相似性
短句来源
    AIM:To study the allelic zymogram of periodic B.malayi from Zhejiang Province.
    目的:研究浙江省周期型马来丝虫等位基因酶谱
短句来源
    CONCLUSION:The allelic zymogram of periodic B. malayi from Zhejiang Province was similar to that of periodic B. malayi from Guizhou Province but passaged in the laboratory.
    结论:浙江省与实验室传代的周期型马来丝虫的同工酶等位基因酶谱基本相似
短句来源
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  enzyme pattern
    A new method for rapidly detecting restriction enzyme pattern of mycobacterium deoxyribonucleic acid (DNA) by capillary electrophoresis with laser induced fluorescence detection (CE-LIFD) was developed.
    建立了毛细管电泳分离 激光诱导荧光检测(CE LIFD)分析分枝杆菌脱氧核糖核酸(DNA)限制性内切酶谱的新方法。
短句来源
    Restriction Enzyme Pattern Analysis of Mycobacteria DNA by Capillary Electrophoresis with Laser Induced Fluorescence Detection
    毛细管电泳-激光诱导荧光检测分枝杆菌脱氧核糖核酸限制性内切酶谱
短句来源
    In order to compare the activities of isozymes in Raji cells infected by simian AIDS type D retrovirus serotype 1 (SRVI ) with that in noninfected cells, the total cell proteins were extracted by ultrasonic vibrator and the enzyme pattern was analyzed with isoelectric focusing in a vertical polyacrylamide gel.
    为了观察猴艾滋病D型逆转录病毒Ⅰ型(SRV1)感染和未感染的Raji细胞同工酶活性的变化,用超声波破碎细胞,提取细胞内总蛋白,然后用垂直薄层聚丙烯酰胺凝胶等电聚焦电泳对其进行酶谱分析。
短句来源
  “酶谱”译为未确定词的双语例句
    SUBCLONING AND MAPPING OF BamHI C FRAGMENT OF HERPES SIMPLEX VIRUS TYPE 1 DNA
    单纯疱疹病毒Ⅰ型DNA中BamHIC片断结构和功能的研究——Ⅰ.BamHI C片断的次级克隆和酶谱分析
短句来源
    Restriction Endonuclease Analysis of Leptospiral DNA from Different Serogroup and Serovar
    不同血清群型钩端螺旋体DNA的限制性内切酶谱分析
短句来源
    A Comparative Study on the Isoenzyme Patterns of Esterase of Oncomelania Snails in Hubei and Yunnan Provinces
    湖北与云南两省钉螺酯酶同工酶酶谱的比较研究
短句来源
    Results The agarose electrophoresis show that the fragments of BMP2 , pGEM - T and pcDNA3. 1 were 1. 2kbp, 4. Okbp and 5. Okbp respectively.
    PcDNA3.1-hBMP-2质粒经酶切电泳可见1.2kbp的hBMP-2条带和5.0kbp的载体片断,重组质粒酶谱分析与预期一致。
    Cloning and mapping of the rpoB gene in M. tuberculosis
    结核分支杆菌rpoB基因克隆与酶谱分析
短句来源
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  zymogram
The basic enzymatic activity was observed in four zymogram zones.
      
Comparison between varietal purity and zymogram purity in maize
      
The zymogram purity of 5 inbred lines, 7 single cross hybrids and 7 single cross hybrids mixed with their parental lines was examined by a vertical discontinous polyacrylamide gel electrophoresis.
      
It was found that the varietal purity is higher than the zymogram purity and they showed a linear relationship.
      
The regression mefficient (γ) between varietal purity and zymogram purity is 0.898 2.
      
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  enzyme pattern
Restriction Enzyme Pattern Analysis of Mycobacteria DNA by Capillary Electrophoresis with Laser-Induced Fluorescence Detection
      
Compared with AGE, CE is more outstanding in resolution and detection time, and it can be applied as a more effective means to DNA restriction enzyme pattern analysis.
      
Although the tumours were of different histogenetic origin their enzyme pattern after cultivation was rather uniform.
      
We found a tumor-specific enzyme pattern in both tissues, a finding which is in accord with the results published in the literature.
      
This satellite is highly conserved in restriction enzyme pattern and DNA sequence; sequence heterogeneity is about 10%.
      
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Peripheral blood lymphocytes were obtained from twenty normal healthy adults. T and B lymphocytes were separated by E rosette formation. The lactate dehydrogenase (LDH) isoenzyme pattern was studied on each T and B fraction obtained and unseparated lymphocytes after agarose'gel electrophoresis. The result showed that the LDH isoenzyme pattern was different between T and B lymphocytes. The T cells had the following pattern:LDH3> LDH>LDH>LDH>LDH5.. The pattern of the B cells was LDH8>LDH4>LDH2>LDH5 >LDH1. The...

Peripheral blood lymphocytes were obtained from twenty normal healthy adults. T and B lymphocytes were separated by E rosette formation. The lactate dehydrogenase (LDH) isoenzyme pattern was studied on each T and B fraction obtained and unseparated lymphocytes after agarose'gel electrophoresis. The result showed that the LDH isoenzyme pattern was different between T and B lymphocytes. The T cells had the following pattern:LDH3> LDH>LDH>LDH>LDH5.. The pattern of the B cells was LDH8>LDH4>LDH2>LDH5 >LDH1. The T lymphocytes had more H subunits of LDH isoenzyme than the B lymphocytes. The M subunits of LDH isoenzyme in B lymphocytes, however, were higher as compared to the T lymphocytes. M:H equals 0.75 in the T cells and 1.13 in the B cells. The difference of LDH isoenzyme distribution suggests that the biochemical course of glucose metabolism may be different between T and B cells, and can be used as a referential criterion for differentiating human peripheral T cells from B cells.

本文用E花环形成法分离了20例正常健康成人外周血的T、B淋巴细胞,作琼脂糖电泳,研究了T、B淋巴细胞和混合淋巴细胞的乳酸脱氢酶(LDH)同功酶谱型。结果表明,T、B淋巴细胞LDH同功酶的谱型不同。T细胞是LDH_3>LDH_2>LDH_4>LDH_1>LDH_5;B细胞则LDH_3>LDH_4>LDH_2>LDH_5>LDH_1。T细胞LDH同功酶中H亚单位较B细胞为多;而B细胞LDH同功酶中M亚单位较T细胞为多。T细胞M:H=0.75;B细胞M:H=1.13。本文认为这种LDH同功酶分布的不同提示T、B细胞糖代谢的生化过程可能不完全相同,并可作为区分人外周血T、B淋巴细胞的一项参考指标。

The nonparticulate fraction of homo-genates of normal adult, fetal and atrophictestes was subjected to agarose gel elec-trophoresis. LDH_x, sandwiched in betweenLDH_3 and LDH_4, was found only in nor-mal adult testes, representing about15. 1% of the total LDH activity. Beingan important tissue-specific molecularform of LDHs in spermatophore, sperm andsemen, LDHx might bear some signifi-cance in spermatogenesis and in fertility aswell. any lowering in its relative activi-ty or its total absence, as in atrophictestis,...

The nonparticulate fraction of homo-genates of normal adult, fetal and atrophictestes was subjected to agarose gel elec-trophoresis. LDH_x, sandwiched in betweenLDH_3 and LDH_4, was found only in nor-mal adult testes, representing about15. 1% of the total LDH activity. Beingan important tissue-specific molecularform of LDHs in spermatophore, sperm andsemen, LDHx might bear some signifi-cance in spermatogenesis and in fertility aswell. any lowering in its relative activi-ty or its total absence, as in atrophictestis, would certainly reflect a correspondlng change in the functional state ofthe testes. A perspective on its role inantisterility research was thus predicted

以成人正常睾丸对照胎儿及成人萎缩睾丸,用琼脂糖凝胶电泳法检测了成人睾丸匀浆中LDH同工酶酶谱。只有成人睾丸匀浆中有一特异同工酶区带-LDHx,介于LDH_3与LDH_4之间,其相对活性为15.1%。LDHx作为精原细胞、精子及精液内LDH同工酶的特异分子形式,它对精子发生及生育能力具有重要意义,因此LDHx的消失或相对活性的降低都将反映睾丸组织生育功能的改变。LDHx的检测也展示了对抗生育研究的前景。

This paper reports the results on the observation and analysis of the morpho-logy, chromosome and isozyme of Paragonimus westermam recovered from Chongan County, Fujian Province. According to the size and morphology of cysts, adults nd eggs, there are both large and small strains of P .westermam as described by He Yi-xun (1981) in the endimic area. Most of them belonged to the small strain of P.westermani and their chromosome number is only 2n=22. In the large strain it is not only 2n=22 but also 3n=33, however,...

This paper reports the results on the observation and analysis of the morpho-logy, chromosome and isozyme of Paragonimus westermam recovered from Chongan County, Fujian Province. According to the size and morphology of cysts, adults nd eggs, there are both large and small strains of P .westermam as described by He Yi-xun (1981) in the endimic area. Most of them belonged to the small strain of P.westermani and their chromosome number is only 2n=22. In the large strain it is not only 2n=22 but also 3n=33, however, the morphology and size of their adult worm have no obvious difference except for their seminal receptacles. Though there were seminal receptacles filled with spermazoa in the diploid-type adult worm from both large and small strains,there is significant differences in size. In the large strain of adult worm two-band, three-band and four-band in MDH were detected by PAGE.

本文报道福建崇安地区的卫氏并殖吸虫形态、同工酶和染色体观察和分析的结果。1。根据囊蚴、成虫和虫卵的大小和形态,该地区的卫氏并殖吸虫有大小两种品系,但以小品系为主;2.根据染色体检查结果表明,小品系卫氏并殖吸虫的染色体仅有二倍体型(2n=22)。大品系中不仅有二倍体型而且还有三倍体型(3n=33),除受精囊外,其成虫的大小和形态无差别(P>0.05)。然而,大小两种品系中的二倍体型的成虫虽均有充满精子的受精囊,但其大小有明显差别(P<0.01);3.大品系成虫的MDH酶同工酶检测结果显示有2条酶带、3条酶带和4条酶带三种酶谱

 
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