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western杂交
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  western blotting
     Western blotting farther proved that GIG138P and GIG138P-G247D were expressed in S.lividans TK54.
     Western杂交进一步证实GIG138P和GIG138P G2 47D在变铅青链霉菌TK5 4中获得了表达
短句来源
     Western blotting further revealed that LHCII apoproteins in W1 dropped to 1/3 of its amount in wild type.
     Western杂交进一步表明,W1的LHCII含量仅为野生型的1/3。
短句来源
     The expression of Annexin A2 on the monocytes membrane was observed by Western Blotting and the binding situation of Annexin A2 withβ_2GPⅠ/anti-β_2GPⅠon the monocytes mambrane was investigated by cellular immunochemistry staining.
     利用W estern杂交技术检测单核细胞膜表面AnnexinA2的表达; 采用免疫细胞化学染色判断单核细胞膜表面Annexin A2、β2GPⅠ、抗β2GPⅠ抗体的结合情况。
短句来源
     Bax and Bcl2 expressions were evaluated by reverse transeriptase PCR(RT-PCR),immunohistoehemistry and western blotting.
     RT-PCR及Western杂交检测Bax、Bcl2基因和蛋白表达。
短句来源
     The results of Western blotting showed that the 120 and 170kDa proteins were the main ones that bound Cry 1 Ac in the BBMV, and the protein at 120kDa was the APN anchored by GPL By using ligand blotting test, a 120kDa protein in BBMV was recognized binding with 5I-Cryl Ac, it was reconfirmed that the 120kDa APN is the receptor for Cry 1 Ac.
     Western杂交证明棉铃虫中肠BBMV上结合Cry1Ac的主要是120和170kDa的蛋白,并且120 kDa蛋白是通过GPI锚定的APN。 通过配体杂交试验,得到~(125)I-Cry1Ac与BBMV杂交的120kDa条带,再次确认这个120kDa的APN是Cry1Ac的受体蛋白。
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  western hybridization
     Moreover, the result of Western hybridization showed that the positive hybridization bands in each scope were that of NGF, BDNF, GDNF;
     而Western杂交结果表明,在上述各Rf范围均有强弱不等的多条阳性杂交带。 分别为NGF、BDNF、GDNF者;
短句来源
     Phosphorylation change of p34cdc2in mouse oocyte during maturation in vitro was detected by Western hybridization.
     通过 Western杂交检测了小鼠卵母细胞体外成熟过程中 p34cdc2的磷酸化变化情况。
短句来源
     The simple protein band fishing by cell (SPBFC) technique was employed in finding the protein bands with neurons attached, and then such protein bands, were taken and transferred to nitric cellulose paper for Western hybridization analysis by antibody for NGF, BDNF,NT-3 and GDNF respectively.
     将有神经细胞贴附的各Rf范围凝胶剪下并将胶内各蛋白带电转移至硝酸纤维膜上,用NGF、BDNF、NT-3、GDNF兔抗血清于硝酸纤维膜上分别进行Western杂交
短句来源
     Serum, of transgenic pig with mini human serum albumin gene, was detected by agarose difussion, protein electrophoresis, Western hybridization and so on We had discovered 3 trangenic pigs to express human serum albumin differently The highest level is 20 mg/mL
     整合有人血清白蛋白基因的转基因猪 ,经琼脂糖扩散、蛋白质电泳、Western杂交等分析 ,发现 3头转基因猪不同程度地表达了人血清白蛋白 ,其中最高表达水平为 2 0 3mg/mL。
短句来源
     The Western hybridization experiment by the probe gave the evidence that the midgut of Chilo suppressalis exists CrylAb receptor protein or proteins.
     用该探针进行Western杂交实验证实了二化螟中肠存在Cry1Ab受体蛋白;
短句来源
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  “western杂交”译为未确定词的双语例句
     (5) Western blot anlysis was taken to test the expressions of PKC α , PKCβ_1, PKC β_2, PKC γ , PKC ε , PKC δ , ERK1/2, JNK1/2, P38, P53, Bax, Bcl2 and Fas in neonatal cardiac cells.
     (5)运用Western杂交的方法分析以下信号分子的表达变化:PKCα、PKCβ_1、PKCβ_2、PKC γ、PKCε、PKC δ、ERK1/2、JNK1/2、P38、P53、Bax、Bcl2和Fas。
短句来源
     (3) The expressed protein of pGEX-5X-p93, pET28a-p93 and pET28a-p93-C were correctly products confirmed with Western blot.
     (3)Western杂交检测后确认pGEX一SX一p93、pET28a一p93和pET28a一P93一C的表达产物均为正确产物。
短句来源
     The expressions of TGFβRⅠ mRNA, 1α (Ⅳ) precollagen, fibronectin(FN) mRNA and TGFβRⅠ protein were measured by reverse transcription polymerase chain reaction (RT PCR), Northern blot and Western blot, respectively.
     分别采用逆转录聚合酶链反应(RTPCR)、Northern和Western杂交检测肾皮质中TGFβRⅠ、1α(Ⅳ)前胶原和纤维连接蛋白(FN)mRNA及TGFβRⅠ蛋白表达。
短句来源
     The expression of α-SMA ,TGF-β1, Smad2/3 , Smad7 and ColⅠ in peritoneal membrane was detected with confocal microscope by immuno-flurence,Western-blot and RT-PCR.
     用RTPCR及间接免疫荧光、Western杂交的方法检测αSMA、TGFβ1、Smad3、Smad7、pSmad2/3、ColⅠmRNA和蛋白的表达水平。
短句来源
     protein expressions of TGFβ 1 and TβRⅠ was measured by Western blot in renal cortex.
     Western杂交检测各组肾皮质TGFβ1及细胞膜TβRⅠ蛋白表达。
短句来源
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  western blotting
Western blotting analysis showed that TM3 protein was purified with affinity purification.
      
The recombinant virus was confirmed using PCR, Southern blotting and Western blotting.
      
The cultural supernatant was collected and tested by SDS-PAGE and Western blotting.
      
Western blotting showed that the protein had a high specificity against mouse-anti-Sj14-3-3 monoclonal antibody and rSj14-3-3 had a promising immune reactivity.
      
Western blotting demonstrated that the pool of 26S proteasomes in ascitic carcinoma Krebs-II was twice that in control lung cells and did not significantly differ by total 26S proteasome quantities from the spleen and liver.
      
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  western hybridization
The transcriptional product of β4Gal-T gene was proved by Western hybridization analysis to be due to GST-fusion.
      
Molecular analyses of seven independent transgenic lines as performed by Southern, Northern and Western hybridization revealed integration and expression of the transgene as well as inheritance in the progeny plants.
      
Transgenic status was confirmed by in vitro TI activity, and Southern and Western hybridization assays.
      
In situ Western hybridization: a new, highly sensitive technique to detect foreign and endogenous protein distribution in rice s
      
We have developed a highly sensitive in situ Western hybridization technique to study tissue-specific expression of foreign and endogenous genes in transgenic and non-transformed rice seed.
      
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SDS-PAGE and two-dimensional electrophoresis were performed to analyze the expression of PR-proteins in leaf washing fluid (IWF)from the resistant cultivar(Erik)and the susceptible line(6B699) of wheat 24,48 and 72 h after inoculation with the isolate 86-124 of Pyrenopora tritici-repentis(ptr).The results showed that infection by the pathogen clearly induced the elevated synthesis of 22 pathogen-related proteins (PR),of which 20 peaked around 48 h after inoculation and declined at 72 h post-infection in both...

SDS-PAGE and two-dimensional electrophoresis were performed to analyze the expression of PR-proteins in leaf washing fluid (IWF)from the resistant cultivar(Erik)and the susceptible line(6B699) of wheat 24,48 and 72 h after inoculation with the isolate 86-124 of Pyrenopora tritici-repentis(ptr).The results showed that infection by the pathogen clearly induced the elevated synthesis of 22 pathogen-related proteins (PR),of which 20 peaked around 48 h after inoculation and declined at 72 h post-infection in both the resistant and susceptible lines.The Western blot indicated that 7 PR-proteins wers β-1,3-Glucanase,4 were Chitianse and 1 was a kind of PR1, suggesting nonspecifical resistance. There were two proteins(pI 5.2,22 kd and pI 6.6,19 kd)which were produced only by the susceptible line upon infection by the pathogen(Ptr ).These two proteins were directly extracted from the 2-D gels and sequenced.

采用SDS-聚丙烯酰胺凝胶电泳及双向电泳技术对小麦抗、感品系在接种褐斑病菌(Ptr)86-124小种后24,48,72h叶片细胞间洗脱液的蛋白质进行了系统的动态研究。结果表明,寄主植物在受到病原物侵染后,22种病原相关蛋白(PR)被诱导合成,接种后48h,PR蛋白的表达量达最大值,72h后相应减少。其中20种组分在抗、感品系间的表达动态没有差异。Western杂交分析证明,其中有7种为β-1,3-葡聚糖酶,4种为几丁酶,1种为PR-1蛋白,属非特异性抗性反应。研究还发现pI5.2、22kd和pI6.6、19kd的两种蛋白仅在感病品系内被诱导合成,与特异抗性反应有关。从凝胶中回收了这两种组分,并完成其氨基酸序列分析。

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利用流式细胞学检查及Southern、Western杂交技术对中国人肝癌细胞SMMC7721进行分析,发现该细胞DNA指数为0.8l,为亚倍体细胞。该细胞Rb基因的5′端存在内部缺失,Rb基因失活,没有Rb蛋白产生。将正常人RbcDNA正向插入逆转录病毒载体DOL,得到重组逆转录病毒载体DOLRB。用电穿孔转染技术把DOLRB转入SMMC7721细胞,发现Rb基因导致约75%的肝癌细胞死亡,另外25%的肝癌细胞则因外源性Rb基因突变失活而继续存活。

Two types of ribosome-inactivating proteins(RIPs) were found to exist in the seed of Cinnamomum camphora,which termed as cinnamomin and camphorin. After the purification of these two RIPs,their dynamic changes and some properties were investigated in this paper. Nondenaturing polyacrylamide gel eletrophoretic and Western blotting results showed that the content of cinnamomin in the seed of Sept.,Oct. and Nov. was 8. 9 %,26. 8 % and 11. 5 % respectively,among which cinnamomin was maximally synthesized in the...

Two types of ribosome-inactivating proteins(RIPs) were found to exist in the seed of Cinnamomum camphora,which termed as cinnamomin and camphorin. After the purification of these two RIPs,their dynamic changes and some properties were investigated in this paper. Nondenaturing polyacrylamide gel eletrophoretic and Western blotting results showed that the content of cinnamomin in the seed of Sept.,Oct. and Nov. was 8. 9 %,26. 8 % and 11. 5 % respectively,among which cinnamomin was maximally synthesized in the seed of Oct. Meanwhile,camphorin content was 1. 7% (Sept),2. 5 % (Oct) and 4. 6% (Nov),gradually increasing with the maturation of seed. These results suggest that the syntheses of cinnamomin and camphorin are regulated by developmental process. In addition,no cinnamomin and camphorin were detected in the leaves,implying that cinnamomin and camphorin are specifically synthesized in the seed. Cinnamomin and camphorin are both glycoproteins,but the carbohydrate content of cinnamomin is 166──fold higher than that of camphorin.

樟树种子中存在着cinnamomin与camphorin两种新的核糖体失活蛋白,电泳分析与Western杂交结果表明cinnamomin在9、10、11月份种子中的含量分别是8.9%,26.8%和11.5%,以10月份种子的含量为最高。camphorin的含量则分别为1.7%,2.5%与4.6%,随着种子的成熟而不断增加。8月份的幼嫩种子中检测不出cinnmamomin与camphorin.这表明樟树核糖体失活蛋白的表达受到了发育进程的时态调控.樟树叶片中可能不存在cinnamomin与camphorin,即两者的合成似乎具有一定的组织特异性.cinnamomin与camphorin均为糖蛋白。

 
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