Methods The cell proliferation, cell cycle progression, cell ultrastructure alterations, mRNA and protein expression of hTERT and TGF-β1 were detected by cell counting, transmission electron microscopy (TEM), flow cytometry (FCM), reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry after T24 and EJ cells were respectively treated with hTERT promoter and mad1 gene plasmids.
Two-hour ischemia later, the expression of GAP-43 mRNA in the rat cerebral tissue was detected with in situ hybridization at day 1, 7, 14 and 30 of reperfusion for observing the effects of the CVB-D on the expression of GAP-43 mRNA, water content, infarct are, behavior scores and cell ultrastructure.
The apoptotic rate was detected by flow cytometry at 1st, 6th, 12th, 18th, 24th hour after hypoxia/reoxygenation was performed and at 12th hour, the expression of apoptosis-related proteins like Bcl-2, Bcl-xl, Bax and P53 in liver cell line L02 was detected. The cellular ultrastructure was observed by using transmission electron microscope.
The DNA content, cellular ultrastructure and the expression of blood group Y antigen and immunosuppressive acidic protein 2(IAP 2) were observed in normal breast, cystic hyperplasia of breast and breast cancer.
Using the method of nutrition culture, the characteristics of aluminum absorption of Longan, the effects of aluminum stress on the growth, cell ultra-structure, some physiological and biochemical response in related to carbon, nitrogen, AOS, and endogenous hormone , content of PAs, and secretion of root was studied in the thesis.
Hypocotyl segments treated with epibrassinolide(1 mg/L)maintained the integrity of ultrastructure of cell, and retarded the senescence, whereas the various organelles of the controls deteriorated after 5 days in vitro.
Comparative studies of HL-60-AR/Nu tumor cells in nude mice and cultured HL-60-AR cells in vitro revealed virtual identity as shown by light microscopic morphology, ultrastructure of cell, cytochemistry, chromosome analysis, LDH isoenzyme pattern, genetic markers and differentiated characters assay.
Conclusion:YRR could scavenge free radical,pro- tect the ultrastructure of cell so as to influence the behavior of smooth muscle cell and endothdial cell in the development of atherosclerosis,and thus to prevent and cure arthemsclemsis.
Methods: On the basis of the model of Pixu rats bearing S 180and EAC solid carcinoma, The rats treated by different doses of Shenmai Injection, Cytoxan and normal saline, were observed by the tumor growth/inhibiting rate, LDH enzyme activity, and ultrastructure of cell under the electron microscope on the level of the whole animal.
At the end of the experiment, the following changes occurred in the cellular ultrastructure: the disappearance of the stored substances and the change in the shape and compression of the physodes.
Using cell culture, cell growth curves of Bel-7402 cells and L-02 cells treated with TiO2 nanoparticles were examined by MTT assay, and the cellular ultrastructure was observed by an analytical transmission electron microscope (ATEM).
The maturation of the endocrine tissue starting on day 19 could be identified by the organization of the adult type of the islets (peripheral A cells) and by the development of the cellular ultrastructure, both being evident on day 20.
Pretreatment of animals with the secretagogue cyclocytidine caused less pronounced damage in cellular ultrastructure and immunological findings, as observed by fluorescence microscopy.
The expression of collagens I and 111, fibronectin, and PH by TCFs was examined by immunohistochemistry, and cellular ultrastructure was evaluated by transmission electron microscopy.