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磷酸二酯键
相关语句
  phosphodiester bond
     Using T_4 RNA ligase,oligonucleotides containing the 2′-5′phosphodiester bondas well as the 3′-5′phosphodiester bond can be synthesized.
     应用T_4-RNA连接酶,可以合成既含有2′-5′又含有3′-5′磷酸二酯键的寡核苷酸。
短句来源
     Evidence provided here thatpA~(2′)p~(5′)A can join with CpUpC,UpCpCpA,Cpm~1IpψpG by the action of T_4 RNA ligasesuggests that oligo(A) with the 2′-5′ phosphodiester bond can be used as a donor forT_4 RNA ligase.
     本文也证实了T_4-RNA连接酶能将供体pA~(2′)p~(5′)A连接到CpUpC、UpCpCpA、Cpm′IpψpG等受体上面去。 从而说明T_4-RNA连接酶也可使用2′-5′磷酸二酯键连接的寡核苷酸作为供体。
短句来源
     As a specific ribonuclease, orientin cleaved only a single phosphodiester bond between the cytidine residue at position 4451 and the adenosine residue at position 4452 in rat 28S ribosomal RNA and hence produced a RNA fragment that is composed of 333 ribonucleotides at the 3' end of rat 28S ribosomal RNA.
     经过RNA酶法测序、引物延伸法测序、RT-PCR、两向薄层层析等方法,我们确定了Orientin是通过水解大鼠核糖体28S RNA第4451位胞苷与4452位腺苷之间的磷酸二酯键来影响核糖体的功能。
短句来源
     Whenthe concentration of Mg2+ is 25 mM in the incubation buffer, the ribonucleasespecifically hydrolyzes the phosphodiester bond between C4453 and A4454 inregion K (a region in domain VII) of 28S RNA in rat ribosome, resulting ininactivation of ribosome.
     本文报道了一种从侧柏成熟种子中提取的新型核糖核酸酶,命名为 Biota orientalis RNase。 Biota orientalis RNase 是一个分子量大约为 13 kDa 的核糖核酸酶,能特异性水解大鼠核糖体 28S RNA上 K 区中 C4453-A4454 之间的磷酸二酯键,导致核糖体失活。
短句来源
     In vivo, there occurs the synthesis and degration of the nucleic acids that concerning the formation and cleavage of phosphodiester bond.
     生物体内核酸的合成与降解都涉及到核酸上磷酸二酯键的生成与断裂。
短句来源
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  the phosphate diester bond
     Meanwhile, research about using rare-earth ions and their complexes as artificial restriction enzymes has gotten more and more recognition, as they can hydrolytically cleave the phosphate diester bond of nucleic acid, and their, cleavage efficiencies are higher.
     与此同时,有关稀土及其配合物作为人工酶的研究越来越受到人们的重视,因为它们能以水解方式断裂核酸中的磷酸二酯键,并且断裂效率较高。
短句来源
     Meanwhile, research about using rare-earth ions and their complexes as artificial restriction enzymes has gotten more and more recognition, as they can hydrolytically cleave the phosphate diester bond in nucleic acid, and their cleavage efficiencies are higher.
     与此同时,有关稀土及其配合物作为人工酶的研究越来越受到人们的重视,因为它们能以水解方式断裂核酸中的磷酸二酯键,并且断裂效率较高。
短句来源
  phosphate diester bond
     Meanwhile, research about using rare-earth ions and their complexes as artificial restriction enzymes has gotten more and more recognition, as they can hydrolytically cleave the phosphate diester bond of nucleic acid, and their, cleavage efficiencies are higher.
     与此同时,有关稀土及其配合物作为人工酶的研究越来越受到人们的重视,因为它们能以水解方式断裂核酸中的磷酸二酯键,并且断裂效率较高。
短句来源
     Meanwhile, research about using rare-earth ions and their complexes as artificial restriction enzymes has gotten more and more recognition, as they can hydrolytically cleave the phosphate diester bond in nucleic acid, and their cleavage efficiencies are higher.
     与此同时,有关稀土及其配合物作为人工酶的研究越来越受到人们的重视,因为它们能以水解方式断裂核酸中的磷酸二酯键,并且断裂效率较高。
短句来源
  “磷酸二酯键”译为未确定词的双语例句
     While [Co(phen) 3] 2+/3+ binds ssDNA probably via electrostatic mode with [Co(phen) 3] 2+/3+-backbone interaction.
     而 [Co( phen) 3 ]2 +/ 3 +则是以静电模式与 ss DNA的磷酸二酯键 PO2 及脱氧核糖组成的骨架相互作用 .
短句来源
     Methods: Eight DRz targeting different sites of PrM gene were designed and synthesized according to the characteristic sites 5′…R↓Y…3′(R=A/G,Y=U/C)which were between an unpaired R and a paired Y location in the secondary structure.
     方法:根据脱氧核酶(DR z)裂解RNA靶位的特异性,选择DEN2 PrM基因中符合5′…R↓Y…3(′R=A/G,Y=U/C)特征且其二级结构中以未配对与配对碱基的磷酸二酯键为靶位,设计合成DEN2特异性脱氧核酶,筛选具有裂解PrM RNA活性的DR z。
短句来源
     by T_4 RNA ligase. The opinion was incurred that oligonucleotides with the 2′-5′phosphodiester bondcan scarcely be used as a donor for T_4 RNA ligase.
     2′-5′磷酸二酯键连接的寡核苷酸能否作为T_4-RNA连接酶的供体,有人估计不大可能。
短句来源
     The rate constants relative to spontaneous hydrolysis of PNPP2Na increased about 120 times.
     PACC对PNPP2Na中的磷酸二酯键有较强的水解作用,其表观速率常数为PNPP2Na自降解的120倍。
短句来源
     1. Biota orientalis ribonuclease specifically cleaves a single phosphodiesterbond in rat 28S ribosomal RNA and inactivates ribosome: A uniqueribonuclease named Biota orientalis ribonuclease (Biota orientalis RNase) ispurified to homogeneity from mature seeds of oriental arborvitae (Biotaorientalis).
     本论文共分为三部分:第一部分Biota orientalis RNase 特异性切割大鼠核糖体 28S RNA 中 C4453-A4454 之间的磷酸二酯键导致核糖体失活:大鼠核糖体 28S RNA 中的 sarcin/ricin 结构域高度保守,该结构域参与了延伸因子(elongation factors, EF-1 和 EF-2)与核糖体的相互作用。
短句来源
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  phosphodiester bond
The enzyme specifically splits the interpolymeric phosphodiester bond between Kp and the 5′-terminal phosphate group of the uridylic acid residue in the Kp-pUpUpGp complex.
      
VPg unlinkase is an unusual eukaryotic enzyme that catalyzes hydrolysis of the phosphodiester bond between residues of the unique tyrosine of VPg (viral protein genome-linked) and the 5"-terminal uridylic acid of picornavirus RNA.
      
We conclude that the whole VPg, when bound to trinucleotide (but not to heptanucleotide), protects the inter-polymeric phosphodiester bond against hydrolysis of the covalent linkage unit.
      
DNA duplexes containing a single phosphoryldisulfide link in place of the natural internucleotide phosphodiester bond were employed in affinity modification of Cys142 in cytosine-C5 DNA methyltransferase SsoII (M.SsoII).
      
The presence of an inverted 3'-3'-phosphodiester bond at the 3'-end of the oligodeoxyribonucleotide only slightly affected the RNase H activity.
      
更多          


By a modified phosphodiester method several oligodeoxyribonucleotides (d-pCpC, d-pOpCpO, d-pCpCpCpA)were synthesized chemically.

用改进的二酯法化学合成了几个脱氧寡核苷酸片段(d-pCpC,d-pCpCpC,d-pCpCpCpA)。改进的主要之点是:(1)全部脱去保护基后柱分离,以克服在柱分离时寡核苷酸的保护基引起的亲脂性;(2)对所需的寡核苷酸片段重上适当的保护基,以作下一步的合成之用,于是避免了原先二酯法的操作中保护基的不希望有的丢失。用改进二酯法获得的寡核苷酸,产率和纯度得到提高和改善。虽然此改进目前仅用来合成寡核苷酸短片段,但可能提供用3’-5’磷酸二酯键方式连接两天然DNA短片段的途径。

By a modified phosphodiester method several oligodeoxyribonuoleotides (d-pCpC, d-pCpCpC, d-pCpCpCpA)were synthesized ohemically.The main points of the modification are: (1) deproteotion of all the protecting groups in order to overcome difficulties encountered in the isolation of oligonuoleotides on column chromatogra.phy,due chiefly to the lipophilic properties of the protecting groups. (2) the appropriate reprotection of the desired oligonuoleotides necessary for the next condensation to avoid the unexpected...

By a modified phosphodiester method several oligodeoxyribonuoleotides (d-pCpC, d-pCpCpC, d-pCpCpCpA)were synthesized ohemically.The main points of the modification are: (1) deproteotion of all the protecting groups in order to overcome difficulties encountered in the isolation of oligonuoleotides on column chromatogra.phy,due chiefly to the lipophilic properties of the protecting groups. (2) the appropriate reprotection of the desired oligonuoleotides necessary for the next condensation to avoid the unexpected loss of the proteoting groups that might have occurred in the previous operations of the phosphodiester method. Thus the yields of the obtained oligonucleotides are increased and the purities improved.Although the present modification has only been applied to the synthesis of short oligonucleotides , it may provide an alternate approach to the chemical condensation of two short natural segments of DNA through the formation of the 3'-5'phosphodi eater bond.

用改进的二酯法化学合成了几个脱氧寡核苷酸片段(d-_pC_pC,d-_pC_pC_pC,d-_pC_pC_pC_pA)。改进的主要之点是:(1)全部脱去保护基后柱分离,以克服在柱分离时寡核苷酸的保护基引起的亲脂性;(2)对所需的寡核苷酸片段重上适当的保护基,以作下一步的合成之用,于是避免了原先二酯法的操作中保护基的不希望有的丢失。用改进二酯法获得的寡核苷酸,产率和纯度得到提高和改善。虽然此改进目前仅用来合成寡核苷酸短片段,但可能提供用3′-5′磷酸二酯键方式连接两天然DNA短片段的途径。

2′-5′oligo(A) is an important mediator in the expression of interferon runction.In this paper the substrate specificity of T_4 RNA ligase and the possibility for thesynthesis of 2′-5′oligo(A) derivatives by T_4 RNA ligase were examined.It was found(1980) that 2′-5′oligo(A) can be used as acceptor for T_4 RNAligase.This problem was further studied here in our laboratories and it was shownthat 2′-5′P_3A_3 can be used as an acceptor in joining with different nucleoside diphos-phates(pNp,N=A,G,G,U);pCpUpC;pCpm_2~2G...

2′-5′oligo(A) is an important mediator in the expression of interferon runction.In this paper the substrate specificity of T_4 RNA ligase and the possibility for thesynthesis of 2′-5′oligo(A) derivatives by T_4 RNA ligase were examined.It was found(1980) that 2′-5′oligo(A) can be used as acceptor for T_4 RNAligase.This problem was further studied here in our laboratories and it was shownthat 2′-5′P_3A_3 can be used as an acceptor in joining with different nucleoside diphos-phates(pNp,N=A,G,G,U);pCpUpC;pCpm_2~2G etc.by T_4 RNA ligase.The opinion was incurred that oligonucleotides with the 2′-5′phosphodiester bondcan scarcely be used as a donor for T_4 RNA ligase.Evidence provided here thatpA~(2′)p~(5′)A can join with CpUpC,UpCpCpA,Cpm~1IpψpG by the action of T_4 RNA ligasesuggests that oligo(A) with the 2′-5′ phosphodiester bond can be used as a donor forT_4 RNA ligase.Using T_4 RNA ligase,oligonucleotides containing the 2′-5′phosphodiester bondas well as the 3′-5′phosphodiester bond can be synthesized.Evidence was also obtainedto show that A~(2′)p~(5′) A can be used as the substrate of T_4 polynucleotido kinase.

在干扰素的功能表达中,2′-5′寡聚腺苷酸是一类重要的媒介物。本文研究了T_4-RNA连接酶的专一性及其用于2′-5′寡聚腺苷酸衍生物合成的可能性。1980年,我们曾发现2′-5′寡聚腺苷酸可以作为RNA连接酶的受体。本文对此作了进一步的研究,证实了T_4-RNA连接酶可以将pNp(N=A,G,C,U)、pCpUpC、pCpm_2~2G等供体连到2′-5′P_3A_3受体上去,生成各种相应产物。2′-5′磷酸二酯键连接的寡核苷酸能否作为T_4-RNA连接酶的供体,有人估计不大可能。本文也证实了T_4-RNA连接酶能将供体pA~(2′)p~(5′)A连接到CpUpC、UpCpCpA、Cpm′IpψpG等受体上面去。从而说明T_4-RNA连接酶也可使用2′-5′磷酸二酯键连接的寡核苷酸作为供体。应用T_4-RNA连接酶,可以合成既含有2′-5′又含有3′-5′磷酸二酯键的寡核苷酸。本工作还证明A~(2′)p~(5′)A也可以作为T_4-多核苷酸激酶的底物。

 
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