The full-length CDS of Parkin and OGCP were amplificationed from the human fetus brain cDNA library and were cloned to the pCMV-Tag4 vector and pcDNA3.1-myc-his(-)B vector differentialy to make the eukaryotic expression vector pCMV-Parkin and pcDNA3.1-myc-OGCP.
Distribution of calbindin (CB)-and parvalbumin (PV)-containing neurons in areas 17and 18 of the visual cortex of 28-week human fetus was investigated by means ofimmunocytochemistry.
Results: ①The cells of the cerebral cortex in the human fetus from 4 to 6 months might survive after cryopreserving and the survival rate in the fetus aged 4 month was higher than that in the fetus aged 5 and 6 months.
Gastric mucosa from 10 human fetuses, 13.5-30 weeks of fertilized age were obtained for studying the distribution and morphology of gastric enterochromaffin cells (EC cell) and gastrin cells (G cell) by protein A gold silver (PAGS) method.
In order to investigate the development of the lung, the ultrastructure of alveolar epithelium from 24 human fetuses of 14-40 weeks in gestation age were observed.
【Objective】 To investigate the expression and distribution of surfactant protein A(SP-A) and its important function during the development of epithelial cells in human fatal lungs.
In this study, we further isolated pluripotent stem cells from human fetal heart, liver, muscle, lung, derma, kidney, and fat and then analyzed the characteristics and function of these stem cells.
Little is known about the expression characteristics of the various kinds of possible markers in hepatic stem cells (HSCs) and other HSC-related cells in human fetal liver in various developmental stages.
Expression of transcription factors PAX6, PROX1, and PITX2 was evaluated in eye tissues after 9.5 and 22.0 weeks of human fetus development using polymerase chain reaction.
For the first time, primers constructed on the basis of OCT4 and NANOG mRNAs were used for PCR analysis of cDNA derived from the eyes of a 9.5-week human fetus.
The pattern of the PITX2 gene expression was studied in the cornea, lens, retina, iridocorneal complex (ICC), and eye coats of human fetuses at weeks 9.5-22 of intrauterine development.
Objective: To study the expression and inducibility in vitro of adrenal cytochrome P-450 (CYP) 1A1 implicated in precarcinogen and premutagen activation in human fetuses.