and the areas under ROC curve of red blood cell,lymphocyte and mid cell count for differentiating S 3-4 from S 1-2 were 0.414,0.436 and 0.502,which were similar to that under the diagnostic reference line( P >0.05).
[Methods] Cultured in BPA of different concentrations(10-4、10-5、10-6、10-7、10-8、10-9、10-10 mol/L),MCF-7 cells were studied in cell proliferation by cell counting and mRNA expression of oncogene EphA2 & c-Myc by RT-PCR.
The 3 H -TdR incorporation and cell counting of rabbit's vascular ECs were inhibited by paclitaxel of 10 nmol·L -1 -1 μmol·L -1 and migration by paclitaxel of 1 nmol·L -1 -1 μmol·L -1 in a concentration-dependent manner (n=6, P< 0.01 ).
Western blot was employed to determine their effect on VDR protein expression and 1,25(OH)_2D_3-induced phosphorylation of PKC. Cell counting and MTT were used to evaluate their influence on the inhibitory effects of 1,25(OH)_2D_3 on HOS-8603 cells proliferation.
Cell number, A value and [ 3H]-TdR incorporation in group probucol+bFGF and group probucol+H 2O 2 were reduced by 40.0%, 39.1%, 45 5% and 46 9%, 45 0%, 39 5%, respectively, compared with group bFGF and group H 2O 2 ( P< 0 05, P< 0 01, respectively).
【Methods】OS732 was treated with 10-5MATRA for 6 days, then weobserved cell morphology, counted cell number, analyzed cell cycles by flow cytometry and detected mRNA expres-sions of cyclinD(D1, D2, D3), CDK4, P21WAF1 by semi- quantity RT- PCR.
Results: (1)Compared with the control group, the MTT OD value and cell number counting were increased to 111.88%,108.05% and 246.11%,122.7% after giving AngII24h and 48h recspectively, theses are significant difference (P<0.01).
Cell proliferation was assessed by cell counting kit-8 (CCK-8).
After subcutaneous injection with SART3 gene vaccine, cytotoxic T lymphocyte (CTL) activity in vitro was measured using Cell Counting Kit-8.
Besides conduct the routine anti-HIV antibody test, more and more laboratories began to conduct other tests, such as CD4+T lymphocyte cell counting, HIV viral load, HIV DNA PCR, genotyping, drug resistance, and HIV-1 recent infection test.
At 0, 30, and 60 min, aliquots were removed for cell counting and nucleotide analysis; 50 μM3H-guanosine was then added and the incubation continued for 1 min.
In addition, CD4+ cell counting is laborious, expensive, and restricted to specialized laboratories.