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  open reading
    Based on the position weight matrix(PWM) and length distribution of open reading frame(ORF),a simple method for predicting translation initiation sites is presented. It can identify TIS from upstream AUGs easily.
    结合位置权重矩阵(PWM,position weight matrix)和开放阅读框架(ORF,open reading frame)的长度分布特征建立了简单的方法识别翻译起始位点,此方法能很好地区分上游AUG和TIS.
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    Sequence analysis showed that the gene of gldABC is 2816bp including three open reading frames encoded polypeptides of 556,194 and 142 amino acids respectively.
    通过对该基因的序列分析,甘油脱水酶(gldABC)结构基因的全长为2816bp,由三个独立的开放阅读框架组成,三个独立的读码框分别由1668、585、426bp组成,分别编码556、195、142个氨基酸。
短句来源
    The open reading frame(ORF)fragments on our microarray were generated by polymerase chain reaction(PCR)using gene- specific primers. Genomic DNA of H.pylori 26695 and J99 ware used as templates.
    以H.pylori 26695和J99作为模板,采用多聚酶链反应(PCR)扩增得到所需要的开放阅读框(open read- ing frame,ORF)片段。
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    Sequence analysis showed that open reading frames of the PB2,PB1 and PA cDNAs contain 2280nt,2274nt and 2151nt encoding 759,757 and 716 amino acids,respectively.
    结果表明,该株病毒的PB2、PB1和PA基因开放阅读框(ORF)分别由2280、2274和2 151个碱基组成,分别编码759、757和716个氨基酸。
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    The cDNA sequence of CYP6F1 has an open reading frame of 1 527 bp coding a putative protein of 508 amino acid residues.
    结果成功克隆出CYP6F1基因,基因全长1639bp,开放阅读框为1527bp,编码508个氨基酸(GenBank登录号:AY662654)。
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    The gene is 1 575 bp in length (GenBank accession number: DQ442997), containing an ORF of 1 326 bp and no intron.
    该基因长1575bp(GenBank登录号为DQ442997),开放阅读框(ORF)长1326bp,无内含子。
短句来源
    The cloned gene had a complete ORF containing 1 896 nucleotides,and encoding 631 amino acid with 73.3 ku of molecular mass.
    同源性分析表明,该基因为日本血吸虫新基因,完整开放阅读框(ORF)长1896 bp,编码631个氨基酸,理论分子质量为73.3 ku。
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    ORF of Rubisco small subunit gene was 186 bp long and encoded 61 amino acids. It was 93%,93%,91%,91% and 92% homologous to the sequences of amino acid in Hordeum capense,Triticum aestivum,Avena clauda,Secale cereale and Bromus cathartic,respectively.
    羊草的Rubisco小亚基基因部分序列含有一个开放阅读框,其长度为186 bp,编码61个氨基酸,与禾本科的小麦、大麦、燕麦、黑麦以及扁穗雀麦Rubisco小亚基基因氨基酸序列的同源性分别为93%、93%、91%、91%、92%。
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    The result showed that the homology of the nucleotide sequence and amino acid sequence of JA TK ORF with other strains was over 99%.
    结果表明,TK基因的开放阅读框(ORF)和所比较的各株的核苷酸和氨基酸同源性都在99%以上。
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    The full-length porcine interferon gamma(PoIFN-γ) cDNA, including the secretion signal peptide coding region was recloned into honor plasmid pFastBac TM 1 of Bac-To-Bac Baculovirus Expression System. These recombinant plasmids, pFastBac TM 1-PoIFN-γ, were transformed into DH_ 10Bac host bacteria to get recombinant shuttle plasmids, rBacmid-PoIFN-γ.
    研究利用Bac-To-Bac杆状病毒表达系统构建含有猪γ-干扰素(porcine interferon-γ,PoIFN-γ)完整开放阅读框的供体质粒pFastBacTM1-PoIFN-γ,转化DH10Bac感受态细胞获得重组穿梭质粒rBacmid-PoIFN-γ,转染sf9昆虫细胞救获表达PoIFN-γ的重组杆状病毒rBac-PoIFN-γ。
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  open reading
Phosphorylation of the Protein Encoded by the First Open Reading Frame of the MDG4 Transposable Element (gypsy) by Homologous an
      
It has been shown that the protein encoded by the first open reading frame (ORF) of the gypsy transposable element (MDG4) is an effective protein substrate both for homologous and heterologous CK2 from the oocytes of Rana temporaria in vitro.
      
It was found that upstream of the EcoRV endonuclease gene two ATG codons give rise to two open reading frames (ORF1 and ORF2) ending at the same point inside the endonuclease gene.
      
This gene contains an open reading frame of 2346 base pairs which encodes a thermostable DNA-polymerase (762 amino acid residues).
      
A BLAST analysis revealed an open reading frame (ORF) that appears to encode for the Tetrahymena DNA-[adenine] methyltransferase ((MTase), based on the presence of motifs characteristic of the enzymes in prokaryotes.
      
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Making use of a dichroic filter type anomaloscope,the ratio and the range of vatiation of red to green light in matching for yellow were Studied in 220 sub jectS(146 males,74 females) with no acquired diseases of COlour vision. of the 220 cases,207 are normal. The statistical properties of the distribution of their R/G ratios were analyzed,assuming a normal distribution.The rest,13 cases, constituting a total of 6% COlour defectives of the sample, consist of 3 protans,3 deutans,1 protanomal,2 deuteranomals and...

Making use of a dichroic filter type anomaloscope,the ratio and the range of vatiation of red to green light in matching for yellow were Studied in 220 sub jectS(146 males,74 females) with no acquired diseases of COlour vision. of the 220 cases,207 are normal. The statistical properties of the distribution of their R/G ratios were analyzed,assuming a normal distribution.The rest,13 cases, constituting a total of 6% COlour defectives of the sample, consist of 3 protans,3 deutans,1 protanomal,2 deuteranomals and 4 extreme deuteranomals. The relative spectral sensitivity cur、vewas determined for 6 of the 7 anomalous trichromats and also for 5 normals. 2 protansand 2 deutans for the purpose of comparison. As an additional qualitative test,the ability of all subjects to tead the pseudo-isOChromatic plates (Ishihara,15 ed.) was also noted. on the basis of a correlated consideration of the present expental findings, the ways by which anomalous trichromatism might come into being were discussed in light of the prpoposal of Roshton regarding the cone pigment trans fomation from normal to dichromats. In order to account for certain behaviour of anomalous trichromats. it has been argued that,in addition to those types inVOlving a pigment transformation,as inthe case of dichromats,of only a proportion of the cell population,some cases probablyinVOlVC an abnormal formation of certain Digments which are not present in either the"red" or the "gteen" system. This abnormal pigment probably represents a certain inter mediate between that of P_(54) and (2P_(54)+3P_(59),thus accounting for a preferential settingof R/G ratio to certain values. In those cases of anomalous trichromat with little or noeviation of the mean value Of R/G ratio from normal but with mederately enlargedatching range,it is likcly that both the "red" and the "green" systems are affected tosome extent so as to make their spectral sensitivity curves approximately equal Therere,besides,even more complicated cases. The 6 anomalous trichromats are thus eterogenous group, DrObably reouiring different explanation for different cases.

利用滤光片型测异仪,对220名(男146,女74)受试者进行了比配黄光所需的红光和绿光的相对量及其变动范围的测定。220例中,207例色觉正常,对他们R/G比值的统计学特性加以分析,假设其分布是正态的。其余13例,占总检查人数的6%,包括甲型色盲3人,乙型色盲3人,甲型异常三色觉者1人,乙型异常三色觉者2人和极端乙型异常三色觉者4人。7名异常三色觉者中的6名的相对光谱亮度曲线曾群加测定。此外,我们还测定了5名正常人,2名甲型色盲和2名乙型色盲的相对光谱亮度曲线,以资此较。同时,所有的受试者还阅读了假等色图谱(Ishihara 15版),作为一种衡量其色分辨能力的定性检查。从我们实验结果的相关考虑,同时根据Rushton对圆锥细胞色素从正常人的分布过渡到二色觉者的分布的建议,对异常三色觉的形成原因进行了探讨。为了解释异常三色觉者的某些视功能表现,除了由一个系统一部分细胞的视色素轉变到另一个系统的视色素而产生的异常三色觉之外,另外一些例子可能是由于形成某些异常的色素而引起的,这种异常的色素既不存在于正常“红”系统中,也不存在于正常“绿”系统中,可能是P_(54)和(2P_(54)+3P_(59))两色...

利用滤光片型测异仪,对220名(男146,女74)受试者进行了比配黄光所需的红光和绿光的相对量及其变动范围的测定。220例中,207例色觉正常,对他们R/G比值的统计学特性加以分析,假设其分布是正态的。其余13例,占总检查人数的6%,包括甲型色盲3人,乙型色盲3人,甲型异常三色觉者1人,乙型异常三色觉者2人和极端乙型异常三色觉者4人。7名异常三色觉者中的6名的相对光谱亮度曲线曾群加测定。此外,我们还测定了5名正常人,2名甲型色盲和2名乙型色盲的相对光谱亮度曲线,以资此较。同时,所有的受试者还阅读了假等色图谱(Ishihara 15版),作为一种衡量其色分辨能力的定性检查。从我们实验结果的相关考虑,同时根据Rushton对圆锥细胞色素从正常人的分布过渡到二色觉者的分布的建议,对异常三色觉的形成原因进行了探讨。为了解释异常三色觉者的某些视功能表现,除了由一个系统一部分细胞的视色素轉变到另一个系统的视色素而产生的异常三色觉之外,另外一些例子可能是由于形成某些异常的色素而引起的,这种异常的色素既不存在于正常“红”系统中,也不存在于正常“绿”系统中,可能是P_(54)和(2P_(54)+3P_(59))两色素轉变过程中停留在中间状态的一种色素。这个假设也可以解释为什么在简单异常三色觉羣里,R/G比值容易处于某些数值。对平均R/G比值与正常人相差很小但此配范围又较大的异常三色觉者,可能的解释是他们的“红”系统和“绿”系统的色素在某种程度上相互轉变,两个系统的吸收光谱相互接近。此外,还有一些更复杂的例子,须要一个以上的形成原因来解释他们的视觉现象。

After injection with latex or plastics, dissections or corrosions of the coronary vessels of 50 rabbits were carried out. The distribution of the arteries and veins of the left ventricle as well as their relationship were observed.Experimental myocardial infarction models were prepared by ligating the anterior descending braneh or the left ventricular branch of the coronary arteries in 20 rabbits. Electrocardiograms were performed before and after Ilgatlon, and anglograms were carried out after tha animals were...

After injection with latex or plastics, dissections or corrosions of the coronary vessels of 50 rabbits were carried out. The distribution of the arteries and veins of the left ventricle as well as their relationship were observed.Experimental myocardial infarction models were prepared by ligating the anterior descending braneh or the left ventricular branch of the coronary arteries in 20 rabbits. Electrocardiograms were performed before and after Ilgatlon, and anglograms were carried out after tha animals were sacrificed.

本文系基础医学系77级学生科研小组于80年用课余及部分假期时间参加科研工作的总结。在教师的指导下,学生阅读有关文献,学习技术操作方法,收集材料,进行观察分析,并初步总结成文,从而得到了科研实践的初步锻炼。这种锻炼不仅取得了一定的科研成果,更使学生学习了有关科研方法,训练严密细致的科研作风,体会科研思维特点,从而提高他们的独立工作能力及增进对科研的兴趣。这对医学生的培养无疑是有益的。 由于学生课余时间有限,与科研工作具有连续性的特点存在一定矛盾。今后应创设条件,一方面鼓励学生积极参加科研工作;另一方面对学生从事科研的要求应灵活多样,如可以参加科研课题的全过程或某个阶段工作、甚或仅参加部分工作。这样既可使学生学得生动活泼,又会对学生的智力开发带来丰硕成果。

Coxsackie B virus antibodies were detected in paired sera of 40 cases of acute viral myocarditis by means of automated, quantitative cytopathic effect (OPE) reading assay of a microneutralization test. The antibody titers were calculated from optical density(OD) reading of crystal violet-stained monolayers on a microelisa auto-reader on the 7th day after incubation. The following equation was used for calculation: 50% CPE = (OD of cell control+OD of virus control)/2. The results were compared with those obtained...

Coxsackie B virus antibodies were detected in paired sera of 40 cases of acute viral myocarditis by means of automated, quantitative cytopathic effect (OPE) reading assay of a microneutralization test. The antibody titers were calculated from optical density(OD) reading of crystal violet-stained monolayers on a microelisa auto-reader on the 7th day after incubation. The following equation was used for calculation: 50% CPE = (OD of cell control+OD of virus control)/2. The results were compared with those obtained from observing the OPE under an inverted microscope for the microneutralization test before staining.Thirty out of 40 cases (75%) showed a fourfold or more than fourfold rising of Coxsackie B virus antibody titers by inverted microscope method for microneutralization test, while 29 out of 40 cases (72.5%) got the same result by automated, quantitative OPE reading assay. Among total 560 detected numbers (40 cases, pair sera, and 7 types of Ooxsackie B viruses), the coincidence rate of these two methods was 96.8%. The automated, quantitative CPE reading assay is quick, sensitive, accurate and gives exact, objective, reproducible data.

本文报道用微量板中和试验以结晶紫染色固定细胞后在微量酶标自动读数仪上按细胞染色深浅所得不同光密度,自动将细胞病变定量读数记录,检测40例急性病毒性心肌炎患者早期及恢复期血清中柯萨奇B组病毒中和抗体,并与微量板中和试验在倒置显微镜下观察结果相比较。二法总符合率为96.8%,但前法有正确、敏感、重复性强、省时、省视力,能快速阅读结果,又有客观记录可资备查等优点。

 
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