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  open reading
    Sequence analysis showed that open reading frames of the PB2,PB1 and PA cDNAs contain 2280nt,2274nt and 2151nt encoding 759,757 and 716 amino acids,respectively.
    结果表明,该株病毒的PB2、PB1和PA基因开放阅读框(ORF)分别由2280、2274和2 151个碱基组成,分别编码759、757和716个氨基酸。
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    Sequencing result demonstrated that the open reading frame(ORF) of the NH36 gene was 945bp,encoding 314 amino acid residues,and the homologies of the nucleotide sequence and amino acid sequence to the published sequence in GenBank were 88.57%(837/945)and 89.17%(280/314),respectively.
    结果显示,获得的NH36开放阅读框为945 bp,编码314个氨基酸残基,与GenBank上发表的国际标准株NH36编码基因序列以及氨基酸序列同源性分别为88.57%(837/945)和89.17%(280/314)。
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    The sequence analysis showed that the nuclotide sequence of the F gene was 1 750 bp,and it contains a single open reading frame(ORF),which encodes a polypeptide of 553 amino acids.
    结果表明,所克隆的基因片段长度为1 750 bp,含有1个1 662bp的开放式阅读框架(ORF),编码553个氨基酸。
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    The mRNA sequence consisted of a 1,251 bp open reading frame (ORF) flanked by a 37 bp 5′-untranslated region (UTR) and a 444 bp 3′-UTR;
    其mRNA序列包含一个1,251 bp的开放阅读框,一个37 bp 的5′非翻译区和一个 444 bp 的 3′非翻译区;
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    The result revealed a DNA fragment of 3 460 bp that contains four complete open reading frames (ORFs).
    结果表明:在全长3460bp的DNA序列中,包含4个完整的开放阅读框(Open reading frame,ORF)。
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    The BC-48 cDNA was 610bp in length with a 570bp ORF encoding 189 amino acids. Similarity of nucleotide sequence of the BC-48 gene with the sequence from GenBank(U46551)was 96.7%.
    结果显示,克隆的BC-48基因片段长610 bp,含有一个570 bp的开放阅读框,编码189个氨基酸,与GenBank中USDA株(U46551)的同源性为96.7%;
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    The results showed that M307 of FUT1 gene digested by Hin6Ⅰcould be divided into two kinds of genotypes, GG and AG.
    结果表明:苏太猪FUT1基因开放阅读框307位点经Hin6Ⅰ酶切后,产生GG型和AG型,不存在抗性纯合子AA型。
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    The result showed that the homology of the nucleotide sequence and amino acid sequence of JA TK ORF with other strains was over 99%.
    结果表明,TK基因的开放阅读框(ORF)和所比较的各株的核苷酸和氨基酸同源性都在99%以上。
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    The full length of the cloned cDNA,was 741 bp with an ORF composed of 639 nucleosides,which encodes 212 amino acids.
    序列分析结果表明:该基因cDNA全长741 bp,开放阅读框由639个核苷酸组成,推测产生的编码产物由212个氨基酸组成。
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    The full-length porcine interferon gamma(PoIFN-γ) cDNA, including the secretion signal peptide coding region was recloned into honor plasmid pFastBac TM 1 of Bac-To-Bac Baculovirus Expression System. These recombinant plasmids, pFastBac TM 1-PoIFN-γ, were transformed into DH_ 10Bac host bacteria to get recombinant shuttle plasmids, rBacmid-PoIFN-γ.
    研究利用Bac-To-Bac杆状病毒表达系统构建含有猪γ-干扰素(porcine interferon-γ,PoIFN-γ)完整开放阅读框的供体质粒pFastBacTM1-PoIFN-γ,转化DH10Bac感受态细胞获得重组穿梭质粒rBacmid-PoIFN-γ,转染sf9昆虫细胞救获表达PoIFN-γ的重组杆状病毒rBac-PoIFN-γ。
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  open reading
Phosphorylation of the Protein Encoded by the First Open Reading Frame of the MDG4 Transposable Element (gypsy) by Homologous an
      
It has been shown that the protein encoded by the first open reading frame (ORF) of the gypsy transposable element (MDG4) is an effective protein substrate both for homologous and heterologous CK2 from the oocytes of Rana temporaria in vitro.
      
It was found that upstream of the EcoRV endonuclease gene two ATG codons give rise to two open reading frames (ORF1 and ORF2) ending at the same point inside the endonuclease gene.
      
This gene contains an open reading frame of 2346 base pairs which encodes a thermostable DNA-polymerase (762 amino acid residues).
      
A BLAST analysis revealed an open reading frame (ORF) that appears to encode for the Tetrahymena DNA-[adenine] methyltransferase ((MTase), based on the presence of motifs characteristic of the enzymes in prokaryotes.
      
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By reading a great deal of medical literat黵e, the author, with his practical ex-perience, gaves a detailed account of the infection and protection of HBY. The systematic data are provided for the control of Hepatitis B.

防治乙型肝炎是我国的一项艰巨工作。本文通过阅读大量文献资料,结合作者在这方面的工作实践,较为详尽地介绍了HBV的感染及防护信息。为防治乙型肝炎提供系统资料。

Using recombinant plasmid pBST2-6 containing enteriotoxigenicEscherichia coli heat-stable

利用含大肠杆菌耐热性肠毒素ST1基因的质粒pBST2-6和含LacZ基因(编码β-半乳糖苷酶)的载体pUC18,构建成ST1-LacZ融合基因。将质粒pBST2-6用限制性内切酶BamHI和BglⅡ消化,经3%琼脂糖凝胶电泳分离、透析袋电洗脱法回收147bpDNA片段,再将载体pUC18用BamHI消化、碱性磷酸酶处理,最后将处理好的pUC18DNA与147bpST1DNA通过T4DNA连接酶进行粘性末端连接,转化至受体菌DH5a中。通过菌落原位杂交筛选,共筛选出53个为ST1基因探针杂交阳性的菌落。菌落原位杂交阳性的菌株经培养和抽提纯化质粒后,经限制性内切酶(EcoRI/XbaI和EcoRI/BamHI)酶切分析和DNA序列分析,证明重组质粒pXST1含有ST1基因,而且ST1基因在重组DNA中连接方向是正确的,具有正确的阅读框架。再者,DH5a(pXST1)菌株能在含x-Gal的LB平板上长成蓝色菌落,表明该菌株能表达具有β-半乳糖苷酶活性的大肠杆菌耐热性肠毒素ST1-β-半乳糖苷酶融合蛋白。ST1-LacZ融合基因的构建,为研究ST的免疫原性和抗ST抗体在预防产肠毒素性大肠杆菌(ETEC)感...

利用含大肠杆菌耐热性肠毒素ST1基因的质粒pBST2-6和含LacZ基因(编码β-半乳糖苷酶)的载体pUC18,构建成ST1-LacZ融合基因。将质粒pBST2-6用限制性内切酶BamHI和BglⅡ消化,经3%琼脂糖凝胶电泳分离、透析袋电洗脱法回收147bpDNA片段,再将载体pUC18用BamHI消化、碱性磷酸酶处理,最后将处理好的pUC18DNA与147bpST1DNA通过T4DNA连接酶进行粘性末端连接,转化至受体菌DH5a中。通过菌落原位杂交筛选,共筛选出53个为ST1基因探针杂交阳性的菌落。菌落原位杂交阳性的菌株经培养和抽提纯化质粒后,经限制性内切酶(EcoRI/XbaI和EcoRI/BamHI)酶切分析和DNA序列分析,证明重组质粒pXST1含有ST1基因,而且ST1基因在重组DNA中连接方向是正确的,具有正确的阅读框架。再者,DH5a(pXST1)菌株能在含x-Gal的LB平板上长成蓝色菌落,表明该菌株能表达具有β-半乳糖苷酶活性的大肠杆菌耐热性肠毒素ST1-β-半乳糖苷酶融合蛋白。ST1-LacZ融合基因的构建,为研究ST的免疫原性和抗ST抗体在预防产肠毒素性大肠杆菌(ETEC)感染中的作用?

The method of the C-band coordinate measure description of domestic pig is reportedfor first time. In the method,the position of evrry C-band appeared at,and the shape ofeach C-band are alphabeted with English letters while thc condition,the frequence and therelative length of each C-band from different chromosome are expressed by numerals. Themethod used table form so as to make every characteristic of C - band described in certain se-quence. Since there exists distinct polymorphism of C-band by itself....

The method of the C-band coordinate measure description of domestic pig is reportedfor first time. In the method,the position of evrry C-band appeared at,and the shape ofeach C-band are alphabeted with English letters while thc condition,the frequence and therelative length of each C-band from different chromosome are expressed by numerals. Themethod used table form so as to make every characteristic of C - band described in certain se-quence. Since there exists distinct polymorphism of C-band by itself. The analysis of C-bandkaryotype is operated using a number of cells instead of using a single cell in order to ob-tain the genuine condition from the individual disparity polymorphism of C-band.It will beconvenient in comparison between the strains and the individuals if this method is put intofuture practice.

本文首次报道家猪染色体C─带带纹量化记述法,该法将每个染色体上C─带带纹的发生位点,发生频率、带纹相对长度、和带形特征采用数据和符号相结合,并以表列的形式依次表述、简单明了、详细精确,它避免了现行方法对其进行逐一描述的繁赘性,和意断性。针对C─带带纹自身的多态性,一改对单个细胞进行分析为对一些细胞进行分析、力求从含行差异的个性中获得反映真值的共性,便于进行品种间和个体间的相互比较,和计算机阅读

 
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