After a primary identification, the halotolerant bacteria screened out is a gram-negative bacteria, streptobacillus, with the shape feature of [(0.6~1)×(2~3)]μm.
Besides, when the enriched halotolerant bacteria was applied into MBBR, the COD removal efficiency of the MBBR could be increased from 20% to 47.5%, which also proved that the halotolerant bacteria screened out has a high capability of specific degradation, and is a effective way in treating the landfill leachate with a high salinity, high toxicity and hardly biodegradable quality.
The values of K_T and K_(IS) are(39.7±27.1)g/L and(329.5±175.8)mg/L,respectively. The dynamic model was testified by actual experimental data,the measured and theoretical values,which indicates that the model can be used to describe the decolorization process of reactive brilliant red K-2BP by salt-tolerant bacteria under high salt concentration conditions completely.
Studies show that the two strains can grow in saturated lime liquid broth (in which the salt-enduring strain can grow in 10% NaCl). The proteinase from them were stable over the pH range from 7 to 11 and be of higher unhairing acivity.
The amount of Ectoine in Halobacillus trueperi SL39 could reach the highest level under the conditions of inducing temperature of 30 ℃,pH 7.0,and NaCl concentration of 2.0 mol/L. After 48 h induction,the concentration of Ectoine was detected as 304.8 mg/L by HPLC,and the extracellular secretion ratio was 90.11%.
The degradation rate follows first order kinetics with respect to dye concentration. Salt inhibition was expressed as a hyperbolic function. The values of K_T and K_(IS) were 39.784±27.169 g L~(-1) and 329.524±175.822 mg L~(-1), respectively.
其抑制常数K_(IS)和K_T值分别为329.5±175.8 mg L~(-1)和39.7±27.1 g L~(-1),说明耐盐菌受盐浓度和染料浓度的双重抑制。
In the phylogenetic tree EC51 is the closest relative to Bacillus Pumilus with 99% sequence similarity. Experiment on the growth condition for the complemental solution of osmotic pressure(Ectoine) with the EC51 strain reveals that the Ectoine synthesized amounts to 197.8 mg/L,with EC51 cultivated for 48 h at 30 ℃ in the bouillon culture-medium including 2 mol/L NaCl.
Studies were carried out on the lipid composition of four strains of extremely halotolerant bacteria belonging to the genus Micrococcus grown at different salt concentrations from 0 up to 4.5 M NaCl.
with eight species of halotolerant bacteria, grown in seawater-irrigated pots under environmental conditions native to the plant's habitat, resulted in significant plant growth promotion by the end of the growing season, 8-11?months later.
Conjugal transfer of a multiresistance plasmid from Pseudomonas fluorescens to halophilic and halotolerant bacteria was studied under in vitro and in situ conditions.
Three salt-tolerant bacteria which degraded xanthan were isolated from various water and soil samples collected from New Jersey, Illinois, and Louisiana.
The exploitation of the salt-tolerant bacteria in the bio-treatment system would be a great improvement of conventional biological treatment systems and the bio-treatment concept.
Natural uncouplers of oxidative phosphorylation, long-chain non-esterified fatty acids, cause uncoupling in the alkalo- and halotolerant bacterium Bacillus pseudofirmus FTU.
Growth yield of the halotolerant bacterium A505 was increased by the supplement of Na+, K+, or Rb+ into the culture media with pH 7.5, and inhibited by Li+ or Cs+.
Salt - enduring strain with a high unhairing protease activity has been isolated from thesalted - pig skins, named Bacillus sp. 813 by identity test. Expriment show that the strain cangrow in 8% NaCl and produces proteases, which are stable in pH7 ～ 11 and have high unhairing protease actVity.
The fermentation conditions of the salt-enduring strain has been studied in this paper.The results indicate that soyabean meal and sweet potato meal are the best nitrogen and carbon sources for the strain to produce proteases.The salt-enduring strain can produce unhairing proteases to the level of 4100-4400u/ml (culture fluid).
The protease from No.813,a recent screened salt-enduring strain,is partially purified through a process of ammonium sulfate preciptation, dialysis,lyophilization.The optium pH and reaction temperature of the obtained proteases is 9.0 and 50℃ for casein hydrolysis,respectively the enzyme is stable over the pH range from 8 to 11,Ca2+ can enhance the stability against heat and pH of enzyme.Its activity was inhibited by Cr3+,Fe2+,Zn2+,Cu2+(1×10-3M),and stimulated by Mn2+.The enzyme is partially inhibited after ...
耐盐菌
halotolerant bacteria
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