助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   同位素碘标记 的翻译结果: 查询用时:0.193秒
图标索引 在分类学科中查询
所有学科
生物学
更多类别查询

图标索引 历史查询
 

同位素碘标记
相关语句
  “同位素碘标记”译为未确定词的双语例句
     It was showed that the radioactivity of 125 I G CSF and 125 I EPO were much higher than that obtained by other methods and it was found that there are high and low affinity EPO receptors in BET 2 cells and one high affinity G CSF receptor in NFS 60 cells. These results are in accord with literature data. It suggests that the two phase iodination of chromine T is an ideal method to iodinate cytokines.
     发现BET2 细胞有高、低两种亲和力的EPO受体, NFS60 细胞只有一种高亲和力的GCSF 受体, 所获结果与文献资料相一致. 说明氯胺T双相碘标法是细胞因子同位素碘标记的理想方法之一.
短句来源
     It suggests that two-phase chloramine-T method is simple and convenient and an ideal method to iodinate protein.
     说明双相氯胺-T碘标法简单方便,是蛋白质同位素碘标记的理想方法;
短句来源
  相似匹配句对
     INDIRECT IODIZATION LABELLING OF CAMODULIN
     钙调素的间接标记
短句来源
     New fast preparation of ~(131)I labelled mIBG
     ~(131)I—间苄胍的快速同位素标记
短句来源
     GISH analysis demonstrated that two E.
     标记E.
短句来源
     Metabolic Study on EGF Labeled Iodine
     标记表皮生长因子的代谢特性
短句来源
     Methods: The isotope labeling method was used.
     方法:采用同位素标记法。
短句来源
查询“同位素碘标记”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
没有找到相关例句


The routine iodination methods are easy to lead to biological inactivation of cytokines, which is one of reasons for failure of ligand receptor binding assay. By using two phase iodination of chromine T, rhG CSF and rhEPO were iodinated with Na 125 I and the characteristic of EPO receptor in BET 2 cell and G CSF receptor in NFS 60 cell were observed by receptor ligand binding assay. It was showed that the radioactivity of 125 I G CSF and 125 I EPO were much higher than...

The routine iodination methods are easy to lead to biological inactivation of cytokines, which is one of reasons for failure of ligand receptor binding assay. By using two phase iodination of chromine T, rhG CSF and rhEPO were iodinated with Na 125 I and the characteristic of EPO receptor in BET 2 cell and G CSF receptor in NFS 60 cell were observed by receptor ligand binding assay. It was showed that the radioactivity of 125 I G CSF and 125 I EPO were much higher than that obtained by other methods and it was found that there are high and low affinity EPO receptors in BET 2 cells and one high affinity G CSF receptor in NFS 60 cells. These results are in accord with literature data. It suggests that the two phase iodination of chromine T is an ideal method to iodinate cytokines.

常规的蛋白质碘标方法易引起被标细胞因子的失活, 是受体配基竞争结合实验失败的原因之一. 试用氯胺T 双相碘标法标记rhGCSF和rhEPO, 并应用受体配基竞争结合分析法测定NFS60 细胞GCSF受体及BET2细胞EPO受体的特性. 结果显示所获125IEPO和125IGCSF放射比活度均较高; 发现BET2 细胞有高、低两种亲和力的EPO受体, NFS60 细胞只有一种高亲和力的GCSF 受体, 所获结果与文献资料相一致. 说明氯胺T双相碘标法是细胞因子同位素碘标记的理想方法之一.

Objective: To establish clot lysis assay and to observe the effect of 32 Chinese medicinal herbs on thrombolysis activity. Methods: Fibrinogen was labelled with 125 I by lactoperoxidase method, and then mixed with platelet free plasma or euglobulin solution. The plasma or euglobulin solution was clotted by adding thrombin, and then incubated at 37℃. The specimen was centrifuged, and radioactivity of the supernatant was measured. Results: (1) The curves of clot lysis time in plasma and euglobulin solution...

Objective: To establish clot lysis assay and to observe the effect of 32 Chinese medicinal herbs on thrombolysis activity. Methods: Fibrinogen was labelled with 125 I by lactoperoxidase method, and then mixed with platelet free plasma or euglobulin solution. The plasma or euglobulin solution was clotted by adding thrombin, and then incubated at 37℃. The specimen was centrifuged, and radioactivity of the supernatant was measured. Results: (1) The curves of clot lysis time in plasma and euglobulin solution exhibited the inverted S shape, the characteristic of enzymatic reaction. Time for 50% lysis (ST50) was about 3.5 days, and 3.5 hours in plasma and euglobulin solution, respectively. (2) The detection of 32 Chinese medicinal herbs with promoting blood circulation by removing blood stasis showed that eight of them such as Paeonia veitchii and Carthamus tinctorius , had thrombolysis activity. Conclusion: It is concluded that both 125 I labelled plasma and euglobulin clot lysis assays are simple, objective and reliable; the former has a wider measure range and more reproducible results; whereas the latter is faster and more sensitive. Some Chinese medicinal herbs with promoting blood circulation by removing blood stasis have the ability to promote fibrinolysis, which deserves to isolate the effectual components from them.

目的 :建立适用于大批量促纤溶活性样品分析筛选的 ,简捷、客观、可靠的实验方法 ,并测定 32种常用活血化瘀中草药的溶栓活性。方法 :采用乳过氧化物酶法将 1 2 5I放射性同位素碘标记到纤维蛋白原上 ,以此作为探针加入到无血小板血浆或优球蛋白溶液中 ,加入凝血酶刺激使之形成栓块 ,经 37℃温育一段时间后 ,离心沉淀未能溶解的纤维蛋白块 ,取一定容积的上清液测定其中的放射性强度从而对栓块的自发纤溶进行定量分析。结果 :(1 ) 1 2 5I-标记血浆和 1 2 5I-标记优球蛋白液所形成栓块的溶解时效曲线均具有酶促反应的倒“S”形特征 ,但前者溶解一半所需时间约为 3.5 d,后者约为 3.5 h。 (2 ) 32种活血化瘀中草药分析观察结果显示 :赤芍、红花等 8种中草药具有一定的促纤溶活性。结论 :1 2 5I-标记血浆和 1 2 5I-标记优球蛋白液栓块溶解法具有简捷、客观、可靠等优点。前者测定的活性范围较大 ,重复性较高 ;而后者则更为快速、灵敏。部份活血化瘀中草药具有明显的促纤溶活性 ,进一步筛选分离出其中的有效成份具有重要的理论意义和应用前景

This research presented a modified two-phase chloramine-T method for radioiodine labeling of protein and Long-lasting Erythropoietin(LL-EPO) was labeled.And traditional gel filtration was substituted by ultrafiltration to purify the labeled protein.The results show that labeled protein possesses high specific radioactivity,biological activity and recovery.It suggests that two-phase chloramine-T method is simple and convenient and an ideal method to iodinate protein.Ultrafiltration is suitable for purification...

This research presented a modified two-phase chloramine-T method for radioiodine labeling of protein and Long-lasting Erythropoietin(LL-EPO) was labeled.And traditional gel filtration was substituted by ultrafiltration to purify the labeled protein.The results show that labeled protein possesses high specific radioactivity,biological activity and recovery.It suggests that two-phase chloramine-T method is simple and convenient and an ideal method to iodinate protein.Ultrafiltration is suitable for purification of the radioiodine labeled protein.

对双相氯胺-T碘标法实验装置作了改进并标记长效促红细胞生成素(LL-EPO),采用离心超滤技术代替传统的柱层析脱盐纯化标记蛋白质。结果显示125I-LL-EPO具有高比活性、高生物活性、高回收率。说明双相氯胺-T碘标法简单方便,是蛋白质同位素碘标记的理想方法;离心超滤技术适合放射性碘标记蛋白质的纯化。

 
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关同位素碘标记的内容
在知识搜索中查有关同位素碘标记的内容
在数字搜索中查有关同位素碘标记的内容
在概念知识元中查有关同位素碘标记的内容
在学术趋势中查有关同位素碘标记的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社