Objective To construct the eukaryotic expression vector for human hypoxia inducible factor-1α gene (pSNAV-HIF-1α), and to investigate the apoptosis and intracellular calcium concentration of the transfected Aβ25-35 induced hippocampal neurons of primary culture.
AIM: To investigate the changes of cytosolic free calcium concentration ([Ca 2+ ] i) and expression of Bcl-2 in HL-60 cells treated by 6F isolated from Pteris semipinnata L. (PSL), and to discuss the relations between calcium ion and cytotoxicity and DNA fragment induction effects of 6F.
Methods Rhodamine-123 fluorescence (very sensitive to mitochondria membrane potential) and Fluo-3/AM fluorescence (suitabe to detect free intracellular calcium in single HaCaT cell) were measured by laser scanning confocal technique.
Involvement of cAMP-Dependent protein kinase and intracellular free calcium ions in regulation of ovulation of the follicle-encl
It was concluded that cAMP-dependent protein kinase and intracellular free calcium ions were involved as second messengers in the gonadotropin regulation not only in maturation of amphibian oocytes but also in ovulation.
on intracellular free calcium in cultured vascular smooth muscle cells of
Both hypoxic endothelial cell conditioned medium and hypoxia elevate intracellular free calcium in pulmonary artery smooth muscl
The concentration of intracellular free calcium ([Ca2+]i) of single cultured ventricular myocyte was determined by using EPC-9 light-electricity measurement system.
We studied the effects of the chelating agents of extra- and intracellular calcium ions, EGTA and BAPTA-AM, and of the inhibitor of Ca2+release from the reticulum, TMB-8, in the kinetics of protein synthesis in hepatocyte cultures.
However, exogenous gangliosides in the dense or diluted cultures preincubated in the presence of BAPTA-AM ore TMB-8 allowed the rhythm visualization, i.e., synchronization may not depend on changes in the intracellular calcium concentration.
Elevation of intracellular calcium level is responsible for activation of ROS-generating enzymes and formation of free radicals by the mitochondria respiratory chain.
On the other hand, an increase in intracellular calcium concentration may be stimulated by ROS.
Unlike FXa, thrombin induced transient intracellular calcium signal in hippocampal neurons, which was mainly mediated via IP3 receptors of the endoplasmic reticulum.
Aequorin measurements indicated that the contractile deficit in the failing myocardium at higher rates of stimulation is associated with decreased free intracellular calcium concentration.
TFA significantly reduced the resting and CaCl2-induced increase of free intracellular calcium concentration ([Ca2+]i) in rabbit plateletin vitro.Conclusion: TFA has an antiplatelet effect via the inhibition on the influx of Ca2+.
The free intracellular calcium concentration was slightly but significantly diminished in patients compared with healthy volunteers.
Application of 100 μM histamine hyperpolarized the resting membrane potential and increased free intracellular calcium.
Phospholipase C-mediated release of inositol trisposphate, followed by an increase in free intracellular calcium, is an important signal transduction pathway for several membrane receptors.