Methods The expression of Beclin 1, Class Ⅰ PI3K (p110α), Class Ⅲ PI3K (hvps34) or p-PKB was, by immunohistochemistry, detected respectively in 25 normal ovarian tissues, 25 benign neoplasia tissues, 19 borderline tissues, and 69 epithelial ovarian carcinoma tissues.
Results: VEGF 121 ,VEGF 145 ,VEGF 165 and VEGF 189 mRNA were detected in normal ovaries and ovarian carcinoma tissues. The expression level of VEGF 121 was significantly higher than that of VEGF 145 , VEGF 165 and VEGF 189 ( P <0.001,respectively).
Methods: 1. RT-PCR was used to detect the expression of RASSF1A ovarian tumor tissues and ascites (or flushing fluid of peritoneal cavity) in 42 malignant ovarian tumor, 11 normal ovarian tissues and 19 benign ovarian tumor tissues.
Northern blot showed that the expression of nm23-H1B was very low in normal ovarian tissues. The expression of nm23-H1B in low malignant ovarian tumors was similar with normal control but higher in ovarian tumor tissues. The level of nm23-H1B gene expression in early stages (Ⅰ and Ⅱ) was higher than that in advanced stages (Ⅲ and Ⅳ).
The positive rates of LPA-2 and LPA-3 receptor mRNA(95.7% and 91.3%) were significantly higher in epithelial ovarian neoplasms than in ovarian benign disease(21.3% and 21.3%) and normal ovarian tissue(15.4% and(15.4%)).
Results The expression of ADM in epithelial ovarian cancer 78.0%(39/50)was higher than that in normal ovarian tissue 10.0%(1/10)and benign ovarian neoplasm 20.0%(2/10),the difference was statistically significance(P<0.01).
[Results] 1) Compared with the normal ovarian tissue , high expression of DNMT1、 DNMT3A、 DNMT3B was observed in primary epithelial ovarian carcinoma (X~2=34.46, 3.86, 14.75, respectively, P<0.005, p<0.05, P<0.005) ;
2)Western blot revealed that the positive rates and expression level of LPA-2 and LPA-3 receptor protein were significantly increased in epithelial ovarian neoplasms compared with ovarian benign disease and normal ovarian tissue(95.7% and 95.7% vs 38.5% and 38.5%,P<0.01).
Relationship among COX-2 protein expression, PGs levels and biologic behavior in ovarian carcinoma tissues
Objective: To study the relationship among cyclooxygenase-2 (COX-2) protein expression, prostaglandins levels and biologic behavior in ovarian carcinoma tissues.
The expression of COX-2 protein and its relative content showed no significant differences in ovarian carcinoma tissues of different clinical stages (I to II and III to IV), different histological grades, with or without ascites and lymph metastasis.
The levels of PGE2, 6-keto-PGF1α and TXB2 showed no significant differences in ovarian carcinoma tissues with different clinical stages (I to II and III to IV), different histological grades, with or without ascites and lymph metastasis.
OVCA680 and OVCA702 were derived from recurrent ovarian carcinomas, whereas others were all derived from primary ovarian carcinoma tissues.
We analyzed the DNA methylation status of ovarian tumors using methylation-specific polymerase chain reaction in 54 frozen ovarian tumor tissues and in 97 cases of archival ovarian serous epithelial tumors using a microdissection procedure.
In addition to these basic findings, elevated expression of KDN was found in fetal human red blood cells compared with adult red blood cells, and ovarian tumor tissues compared with normal controls.
In situ hybridization analysis showed that KLK4 mRNA transcripts are localized to adenocarcinoma cells of ovarian tumor tissues.
External sex characteristics of intersex individuals have been described in detail, while in histological analyses of internal sex characteristics ovarian tissue was found exclusively.
Transposition of the ovaries to allow better shielding during radiotherapy is of use in some women, and the prospect of cryopreservation and reimplanation of ovarian tissue appears to be promising.
The relationship between Zn and Cs when Fe is kept constant could be used as a tool for the decontamination of the ovary from an abnormal Cs content or for the inhibition of the accumulation of the same element to the ovarian tissue.
Ovarian tissue was not damaged to the same extent as testicular cells.
Furthermore, they show that luteoma possess GnRH receptors similar to those in normal ovarian tissue, and that GnRH analogs have endocrine effects on these cells.