Objective To investigate the effects of dexamethasone(DXM) on activation of nuclear factor kappaB (NF-κB) and production of tumor necrosis factor-α (TNF-α) in lipopolysaccharide (LPS)-induced human peripheral blood monocytes.
Method The cultured human peripheral blood monocytes were divided into 4 groups: normal saline control group,LPS treated group,LPS plus DXM 1 μmol/L treated group,LPS plus DXM 10 μmol/L treated group.
The effects of M-CSF and IL-10 on the surface expressions of CD14,CD23,CD64,CD11b,CD18 and CD29 on human peripheral blood monocytes were determined by means of flow cytometry.
The cDNA encoding the extracellular domain of human costimulatory molecule B7 1/CD80 was cloned from human peripheral blood monocytes by using RT PCR method and sequenced by Sanger chain termination method.
Methods:With the Digoxenin-labeled Oligonucleotide-probes In Situ Hybridization,studied the effects of OxLDL on the mRNA expression of proinflammatory cytokines including MCP-1, bFGF, PDGF and IL-10 in the human peripheral blood monocyte.
The effects of monoclonal antibodies(McAb) to core glycolipid(CGL)region of lipopolysaccharides(LPS) on the dynamic change and mRNA expression of Interleukin 1(IL 1)in supernatants of cultured human peripheral blood monocyte(hPBMC) induced by LPS in vitro were studied by cytokine bioassay,Northern blot and RT PCR analyses.
ObjectiveTo develop a Nested-Real-Time-PCR method for the detection of F0XP3mRNA in human peripheral blood mononuclear cells(PBMC), study the correlation of F0XP3mRNA with CD4~+CD25~+ Regulatory T cell, ratio of CD4 and CD8, Th1 and Th2, syndrome types of Traditional Chinese Medicine(TCM).
The effects of cyclosporin A(CyA) and Tripterygium wilfordii on the expressions of IL 6 and IL 8 mRNA of human peripheral blood mononuclear cells(PBMCs) induced by recombinant human IL 1α(rhIL 1α)
Objective: To explore the effects of Lycium barbarum polysaccharide (LBP-X) on IL-2 and TNF-α gene expressions in human peripheral blood mononuclear cells.
Methods: In vitro human peripheral blood mononuclear cell (PBMC) were cultured with wear particles and bisphosphonates of different concentration, then productions of IL-1β and IL-1β mRNA were determined by ELISA and in situ hybridization techniques.
In vitro infection demonstrated that vMIP2 potently inhibited the replication of R5 and X4 HIV in human peripheral blood mononuclear cell (PBMC). This study provides basis for development of effective prevention strategies against HIV. Further investigation may help to define the role of vMIP2 in HHV8 pathogenesis.
Objective To observe the effects of a novel HLA-derived peptide, RDP1258, on the human peripheral blood mononuclear cell (PBMC) proliferation to ConA and MLR, and to investigate the mechanisms.
We found that GM3 levels in human peripheral blood monocytes and cultured monocyte-derived macrophages were 0.37 and 2.7 μg per million cells, respectively.
Objective: This study is aimed at developing a simple and easy way to generate dendritic cells (DCs) from human peripheral blood monocytes (PBMCs) in vitro.
The present study was designed to investigate the capacity of human peripheral blood monocytes to express MCP-1 and effects of native very low density lipoprotein (VLDL) and oxidized VLDL (OX-VLDL) on the expression.
A stable human macrophage hybridoma was established by somatic cell fusion between human peripheral blood monocyte-derived macrophages and an 8-azaguanine resistant clone of a human histiocytic lymphoma cell line U-937 (clone U-937-F9).
We examined the variability of occurrence and frequency of voltage-dependent whole-cell currents in human peripheral blood monocyte-derived macrophages (HMDM) maintained in culture for up to three weeks.
A synthetic peptide was shown to bind to TACE and to inhibit TNF-α release from lipopolysaccharide (LPS)-stimulated human peripheral blood mononuclear cells (PBMC) by up to 60.3%.
Human LAK cells were prepared by culturing normal human peripheral blood mononuclear cells (PBMC) with or without rIL-2 and assayed for T cell surface markers as well as anti-tumor activity against PGin vitro and in nude mice.
Our study showed that deguelin inhibited the proliferation of K562 cell and K562/ADM cell in a time- and dose-dependent manner and had minimal effects on normal human peripheral blood mononuclear cells.
In our previous work, the salivary gland extract (SGE) from Ixodes ricinus ticks impaired T-lymphocyte proliferation and clearly modulated the immune response towards the Th2 pattern in human peripheral blood mononuclear cell culture.
Significant human peripheral blood mononuclear cell (PBMC; n?=?12) proliferation was observed in response to amino acids (AA) 502-521 (peptide 7), located in the catalytic domain of GTF.
IgD production by short-term human peripheral blood mononuclear cell (PBM) cultures was studied to establish thein vitro correlates of low serum IgD expression.