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gene     
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  基因
     Biological and Biochemical Characterization of Envelope Gene of Subgroup J Avian Leukosis Virus
     禽白血病病毒J亚群囊膜糖蛋白基因的生物学和生物化学特性
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     Apoptosis and Expression of Apoptosis Associated Gene in Smooth Muscle Cells of Autogenous Vein Graft in Rat Experimental Model
     移植静脉平滑肌细胞凋亡及相关基因表达与增殖关系的实验研究
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     STUDY ON THE RECOMBINANT DNA VACCINE COEXPRESSING NEWCASTLE DISEASE VIRUS F GENE AND CHICKEN IL-2
     新城疫病毒F基因与鸡IL-2重组DNA疫苗的研究
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     CONSTRUCTION OF SMUT RESISTANT OR SUSCEPTIBLE POOLS AND MOLECULAR MARKER FOR RESISTANCE GENE IN SUGARCANE
     甘蔗抗感黑穗病池的构建和抗病基因分子标记
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     Mapping and characterization of a new tomato resistance gene Cf-ECP3 against Cladosporium fulvum
     番茄抗叶霉病新基因Cf-ECP3的作图定位和特性分析
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  基因的
     Biological and Biochemical Characterization of Envelope Gene of Subgroup J Avian Leukosis Virus
     禽白血病病毒J亚群囊膜糖蛋白基因的生物学和生物化学特性
短句来源
     Molecular Design, Construction and Expression of A Fused Insecticidal Gene
     融合杀虫基因的分子设计、构建及表达研究
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     Cloning of germin gene, synthesizing of human lysozyme gene and their expression in tobacco and oilseed rape
     Germin基因的克隆和人溶菌酶基因的合成及其在烟草和油菜中的表达
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     The Fusion Protein Gene of Newcastle Disease Virus Strain F_(48)E_8:Sequence Analysis and Expression by a Recombinant Fowlpox Virus
     新城疫病毒F48E8株融合蛋白基因和表达该基因的重组鸡痘病毒
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     Cloning of a cDNA fragment of resistance-related gene and the candidate for the stripe rust resistance gene Yr10 from wheat
     小麦抗条锈病相关基因cDNA片段及Yr10候选基因的克隆
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  基因序列
     Phylogenetic Analysis of Bluetongue Viruses in China and S10 Gene Expression of the Virus
     中国蓝舌病毒S10、L2基因序列分析及S10基因克隆表达
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     Comparative Analysis and Molecular Phylogeny of Chinese Deer Based on Mitochondrial 12S rRNA Gene Sequences
     中国鹿类动物线粒体12S rRNA基因序列的比较分析及分子系统发育研究
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     Study on Sequences, Molecular Evolution and Polymorphism of Horse Growth Hormone Gene
     马生长激素(GH)基因序列、分子进化及其多态性的研究
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     Molecular Phylogeny of Pneumocystis Based on mt LSU rRNA and ITS1-5.8SrRNA-ITS2 Gene Sequence
     基于mtLSU rRNA和ITS1-5.8SrRNA-ITS2基因序列的肺孢子虫分子系统发育学研究
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     Study on Detection Methods of Gene Mutation and Their Clinical Applications
     基因序列变异分析的方法研究及其临床应用
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  基因(
     Transformation and Expression of an Avian Influena A (H5N1) Virus Gene(h5nla) in Transgenic Potato——Compared with Expression of Hepatitis B Surface Antigen(HBsAg) in Transgenic Potato
     禽流感病毒(H5N1)基因(h5n1a)在马铃薯中的转化与表达研究——附:乙肝表面抗原(HBsAg)基因转化的对比研究
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     Cloning and Expression of the Ethylene Receptor Gene (etr1) of Hevea brasiliensis
     巴西橡胶树(Hevea brasiliensis)乙烯受体基因(etr1)的克隆及表达分析
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     Cloning of Yeast Gene (Pro2) Coding for Proline Synthesizing Enzyme (Glutamate Phosphate Reductase) and Leu~+ Pro~+ Phenotypic Cotransduction
     酵母脯氨酸合成酶基因(Pro2)的分离及Leu~+ Pro~+表型共转导
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     Experiments of Mammalian Genetic Engineering Experiment 15 DNA Mediated Gene Transfer
     实验15 DNA介导转移基因(DMGT)
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     SUBCLONING OF THE PROMOTOR FRAGMENT OF THE YEAST 3-PHOSPHOGLYCERATE KINASE GENE (PGK1)
     酿酒酵母3-磷酸甘油酸激酶基因(PGK1)启动子片段的亚克隆
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  gene
NEW TECHNOLOGY FOR DRUG DISCOVERY BASED UPON INSERTION OF LIGANDS INTO GENE SEQUENCES BY NUCLEAR RECEPTOR PROTEINS
      
A gene regulatory mechanism has been proposed in which steroid hormones and certain other drugs bind to nuclear receptor proteins followed by transfer to DNA where they are inserted between base pairs.
      
Polymerase chain reaction was used to amplify a 439-bp fragment of a 65,000-kDa (Mr) heat shock protein gene (hsp65) of Mycobacterium.
      
Cloning of an APETALA3 homologous gene (PtAP3) from Populus tomentosa and genetic transformation of its sense and anti-sense con
      
A pair of primers were designed according to published literature on Populus trichocarpa gene (PTD), and PtAP3, an AP3 homologous gene from Populus tomentosa was isolated by PCR using genomic DNA of the male clone of P.
      
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Earias cupreoviridis Walker is one of the serious pests of field cotton in Hunan.This paper deals with the results obtained in1959, 1960 and 1961 in Tar-tong Lake ofHunan province. Five generations were recorded in the laboratory and also observed inthe cotton field in 1961. Earliest appearance of the moth in cotton field was in late June, but larvae in fieldwere abundant from early July to late August (third to fourth generation). The last gene-ration larvae occurred in November from which pupae were...

Earias cupreoviridis Walker is one of the serious pests of field cotton in Hunan.This paper deals with the results obtained in1959, 1960 and 1961 in Tar-tong Lake ofHunan province. Five generations were recorded in the laboratory and also observed inthe cotton field in 1961. Earliest appearance of the moth in cotton field was in late June, but larvae in fieldwere abundant from early July to late August (third to fourth generation). The last gene-ration larvae occurred in November from which pupae were produced within the cottonplant to pass the winter. The longevity of the moth was 4.9--11.5 days. The durationof egg stage was 3--10.1 days. The larvae passed 6 instars and larval stage lasted10.5--23.3 days. The pupal period of the first four generations was around one to twoweeks. The larvae did not remain in one boll or bud but bore out again and crawl frombud to bud or boll to boll. Other habits of adult, larvae and pupa are described. The sequence of outbreak of this pest were also investigated. In general, larval po-pulation increased when average five-day temperature was between 26--30℃. andaverage humidity over 80%, or coefficient of temperature and humidity between 2.7--3.3.On the other hand, population decreases when the average five-day temperature wasover 30℃. and the coefficient of temperature and humidity below 2.7. When the plantswere dense,the percentages of infection were different. The names of varieties of cottonunder observation were given.

棉鼎点金刚钻在湖南每年发生5—6代,以6代为主。第1—2代在早春奇主上发生,6月下旬开始迁入棉田,以第3—6代在棉花上发生,但第3—4代为害较大。各代及各虫态历期,以及各虫态主要习性均曾加以记述。 1959—1961年调查证明:棉田中鼎点金刚钻幼虫盛发期较适宜的气候条件为:每五日平均气温26—30℃、相对湿度80%以上、温湿系数2.7—3.3。密植棉田受害较重。岱福棉、岱红2343受害最轻,宁棉13号、南通一、二号较重。

Field and laboratory investigations on the overwintering forms of five species ofaphids, the cotton aphid, Aphis gossypii Glover, the English grain aphid, Macrosiphumgrandrium (Kirby), the turnip aphid, Rhopalosiphum pseudobrassicae (Davis), the greenpeach aphid, Myzus persicae (Sulzer), and the black citrus aphid, Toxoptera aurantii(Fonsc.) were carried out during 1955--1962 in Chungking, Szechuan. Under natural conditions, the cotton aphids lay overwintering eggs on certain de-ciduous woody plants, such as...

Field and laboratory investigations on the overwintering forms of five species ofaphids, the cotton aphid, Aphis gossypii Glover, the English grain aphid, Macrosiphumgrandrium (Kirby), the turnip aphid, Rhopalosiphum pseudobrassicae (Davis), the greenpeach aphid, Myzus persicae (Sulzer), and the black citrus aphid, Toxoptera aurantii(Fonsc.) were carried out during 1955--1962 in Chungking, Szechuan. Under natural conditions, the cotton aphids lay overwintering eggs on certain de-ciduous woody plants, such as the cotton-rose, Hibiscus mutabilis L., and the shrubbyalthaea, Hibiscus syriacus L. This species, however, will remain in parthenogenetic formsthoroughout the winter season on the herbaceous plant, such as the hollyhock, Althaearosea (L.). The English grain aphid, the turnip aphid and the black citrus aphid gene-rally continue their parthenogenetic generations in winter on their particular host plants.It seems more complicate in the case of the green aphid, those bred on winter growingvegetable crops, such as the various species of crucifers, the lettuce and spinach, usuallyreproduce asexually in winter; but a few sexuparae, sexual females and males may alsoappear on peach leaves. As the sexual females would not lay any overwintering eggwithout mating, and the males usually appear later on peach tree than the sexual females,hence the females are usually incapable of laying eggs before the falling of the peachleaves. Under experimental conditions, when the turnip aphid bred on old yellowing leavesof unhealthy plants of rape at a certain low temperature would produce a few sexualfemales and eggs, but no sexual male was observed. Some results were obtained fromfield investigations on the Chinese cabbage. The English grain aphid when bred arti-ficially on ears of wheat plants, which were sown early in August and reached headingand milky stage early in winter, produced a few males, but no sexual female or eggwas observed. Insectary breeding tests proved that inoculation of the cotton aphids ontohollyhock from field cotton, cotton-rose and shrubby althaea produced parthenogeneticoffspring; when the cotton aphids inoculated onto shrubby althaea twigs, cultured intap water, produced sexual offspring. These results were quite similar to those obtainunder natural conditions. The writer considers that the temperature, the photoperiod and the kinds and growthconditions of host plants will bring a combined effect on the reproduction of sexualforms in aphids, but the effect will be various in different species. Therefore, in thesame locality, different species of aphids may have different overwintering forms becauseof their different reactions to the environmental factors. Moreover, in one districtwhere the environmental conditions are fundamentally alike, under a definite low tem-perature, a species of aphid may either reproduce parthenogenetically or produce sexualforms in winter, depending essentially upon the different nutrient conditions in the dietobtainable by the species from its host plants.

在重庆地区的自然条件下,棉蚜Aphis gossypii Glover在冬季既可以在落叶木本植物如木芙蓉Hibiscus mutabilis L.、木槿 Hibiscus syriacus L.上产生两性世代的雄蚜和产卵雌蚜,并产卵过冬;也可以在宿根草本植物如蜀葵 Althaea rosea(L.)上继续以孤雌胎生世代的有翅或无翅胎生雌蚜繁殖过冬。麦长管蚜 Macrosiphum granarium(Kirby)、菜缢管蚜 Rhopalosiphum pseudobrassicae(Davis)和桔二岔蚜Toxoptera aurantii(Fonsc)通常都继续以孤雌胎生世代繁殖过冬。桃蚜 Myzus persicae(Sulzer)在十字花科蔬菜、莴笋Lactuca sativa var.angustana Irish、菠菜 Spinacia oleracea L.等冬季作物上可以继续营弧雌胎生世代繁殖,但在桃树上Prunus persica Stokes 可以出现少数两性世代的产卵雌蚜和雄蚜。由于雄蚜的出现时期较晚,将近在落叶以前,而产卵雌蚜不经交配通常均不能产卵,因此在桃树落叶前产卵雌蚜常未及产卵而随...

在重庆地区的自然条件下,棉蚜Aphis gossypii Glover在冬季既可以在落叶木本植物如木芙蓉Hibiscus mutabilis L.、木槿 Hibiscus syriacus L.上产生两性世代的雄蚜和产卵雌蚜,并产卵过冬;也可以在宿根草本植物如蜀葵 Althaea rosea(L.)上继续以孤雌胎生世代的有翅或无翅胎生雌蚜繁殖过冬。麦长管蚜 Macrosiphum granarium(Kirby)、菜缢管蚜 Rhopalosiphum pseudobrassicae(Davis)和桔二岔蚜Toxoptera aurantii(Fonsc)通常都继续以孤雌胎生世代繁殖过冬。桃蚜 Myzus persicae(Sulzer)在十字花科蔬菜、莴笋Lactuca sativa var.angustana Irish、菠菜 Spinacia oleracea L.等冬季作物上可以继续营弧雌胎生世代繁殖,但在桃树上Prunus persica Stokes 可以出现少数两性世代的产卵雌蚜和雄蚜。由于雄蚜的出现时期较晚,将近在落叶以前,而产卵雌蚜不经交配通常均不能产卵,因此在桃树落叶前产卵雌蚜常未及产卵而随落叶从桃树上消失。从接种饲养试验的结果证明:麦长管蚜在提早至8月播种,至冬季已进入乳熟期的小麦Triticum aestivum L.穗上,可以产生少数雄蚜,但未见产卵雌蚜。菜缢管蚜在生长衰弱的油菜 Brassica napella Choix的黄脚叶上,可以产生少数产卵雌蚜及卵,但未见雄蚜。在田间瓢儿白Brassica chinensis var

Four different types of cytoplasmic male sterility in maize were tested for their identityby use of the restoration technique.Male-sterile lines incorporated with cytoplasm of theTexas source were found to be completely affected in crosses by a group of restorers includ-ing W153,W28 and G32,and partially restored by W24,M14 and A34.This made a figure of6 restorers out of about 60 inbreds thus tested in the Texas cytoplasm.Inbreds W153 wasselected as a differential restorer for T-type steriles since it gave no...

Four different types of cytoplasmic male sterility in maize were tested for their identityby use of the restoration technique.Male-sterile lines incorporated with cytoplasm of theTexas source were found to be completely affected in crosses by a group of restorers includ-ing W153,W28 and G32,and partially restored by W24,M14 and A34.This made a figure of6 restorers out of about 60 inbreds thus tested in the Texas cytoplasm.Inbreds W153 wasselected as a differential restorer for T-type steriles since it gave no reactions to the othertypes.For the Moldavia type of male sterility,formerly offered by Prof.Hadjinov,we founda partially restoring inbred W9 to be better suited for the similar purpose.The third andfourth type of male sterility,designated as B- and G-type,came through our own selectionsfrom two Bulgarian varieties.Separation between them seemed difficult.Since A374 gavepartial pollen fertility to the B-type steriles exclusively,it could be used as a differentiat orfor this type of cytoplasm.Pollen restoration in crosses involving W153 and W28 followed the expectation based ona dominant Mendelian gene.However,data obtained from segregating progenies of doublecrosses in which G32 was the restorer suggested strongly that two dominant complementarygenes were more workable.The difference in genotype of inbreds concerned in variousinvestigations seemed to be responsible for such inconsistent results.Segregating patterns in the Texas sterile crosses of the partial restorer,W24 or M14,varied with plants used as the pollen parent and with the date of planting.It appeared verylikely that W24 and M14 were heterozygous for major restorer genes since one sterile versionof W24 and four fully restoring lines of M14 had been established by conversion and test-cross-ing respectively.Dominant modifiers might also be present in either of the inbred popula-tions.In the presence of Moldavian cytoplasm the recessive allele of the restorer gene seemedto exert an abortive action to its pollen carriers produced by the heterozygote.When plantsheterozygous for the restorer gone were outcrossed to male steriles carrying the right cyto-plasm,all plants from the progenies proved to be pollen shedders.It was suggested that theM-type cytoplasm might be similar to that of S-type.Tentative genotypes related to pollen restoration of Texas male sterility had been workedout for a number of inbreds on the basis of two dominant complementary genes.Workingschemes for the production of double-cross seeds of maize without detasseling had been sum-marized and discussed by the authers.

三种细胞质遗传的玉米雄花不孕类型各有其专效的恢复系和部分恢复系。在 T 型细胞质基础上,测定出两对显性互补基因决定着花粉孕育性的恢复,同时还有显性修饰基因存在,影响其表现的程度。春播和夏播的不同环境条件只对部分恢复性的表现发生明显影响,对全恢复性和不孕性则很少能够改变。M 型恢复性的等位隐性基因在杂合株内对花粉粒具有某种致死或败育作用,故测交后代表现不分离现象。本文初步鉴定了若干常用自交系的 T 型恢复基因型,并以此作为根据,提出了配制全不去雄的玉米双交种的各种可能方案。

 
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