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gene
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  基因
    Biological and Biochemical Characterization of Envelope Gene of Subgroup J Avian Leukosis Virus
    禽白血病病毒J亚群囊膜糖蛋白基因的生物学和生物化学特性
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    Molecular Design, Construction and Expression of A Fused Insecticidal Gene
    融合杀虫基因的分子设计、构建及表达研究
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    The Fusion Protein Gene of Newcastle Disease Virus Strain F_(48)E_8:Sequence Analysis and Expression by a Recombinant Fowlpox Virus
    新城疫病毒F48E8株融合蛋白基因和表达该基因的重组鸡痘病毒
短句来源
    Preparation of the antifungal protein from Paenibacillus polymyxa WY110 and the coding gene cloning and expression
    多粘类芽孢杆菌WY110抗菌蛋白的制备及其基因克隆和表达
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    Transformation and Expression of an Avian Influena A (H5N1) Virus Gene(h5nla) in Transgenic Potato——Compared with Expression of Hepatitis B Surface Antigen(HBsAg) in Transgenic Potato
    禽流感病毒(H5N1)基因(h5n1a)在马铃薯中的转化与表达研究——附:乙肝表面抗原(HBsAg)基因转化的对比研究
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  基因序列
    Phylogenetic Analysis of Bluetongue Viruses in China and S10 Gene Expression of the Virus
    中国蓝舌病毒S10、L2基因序列分析及S10基因克隆表达
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    Comparative Analysis and Molecular Phylogeny of Chinese Deer Based on Mitochondrial 12S rRNA Gene Sequences
    中国鹿类动物线粒体12S rRNA基因序列的比较分析及分子系统发育研究
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    DNA Sequence of Xylose Isomerase Gene from Streptomyces diastaticus No. 7 Strain M1033
    7号淀粉酶链霉菌M1033木糖异构酶基因序列分析
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    Sequence analysis of TuMV coat protein gene Hangzhou isolate
    芜菁花叶病毒杭州分离株外壳蛋白基因序列分析
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    Analysis of Nucleotide Sequence and Pbylogeny on HA_1 Gene of Influenza B Viruses Isolated in Hebei
    乙型流感病毒河北株的HA_1基因序列及种系发生的分析
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  “gene”译为未确定词的双语例句
    Preparation and Characterization study of Gene Engineering Antibody against Platelets GP
    抗血小板膜糖蛋白基因工程抗体的制备和性质研究
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    Construction of Pseudorabies Virus SH Strain Mutant with gE-gI Gene Partial Deletion Inserting GFP-LacZ Reporter Gene and Primary Study Its Immunogenicity
    伪狂犬病病毒上海株gE~-/gI~-/GFP~+/LacZ~+缺失株的构建及其免疫原性初步研究
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    Gene Transfer of huCTLA4-Ig to Induce Immune Tolerance of Rat Liver Graft
    基因转移huCTLA4-Ig诱导大鼠移植肝免疫耐受的实验研究
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    Preparation of Recombinant Human Glucagon-like Peptide-1 by Gene Engineering and Studies on Its Biological Activities
    基因工程制备重组人胰高血糖素样肽-1及其生物学活性研究
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    Study on the Impact of Hot-spot Mutations in HBV/C Gene on the Immune Response of Mice by Gene Transfer Mediated by Recombinant Adenovirus
    用腺病毒载体介导的基因转移研究HBV/C区热点变异对小鼠免疫应答的影响
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  gene
NEW TECHNOLOGY FOR DRUG DISCOVERY BASED UPON INSERTION OF LIGANDS INTO GENE SEQUENCES BY NUCLEAR RECEPTOR PROTEINS
      
A gene regulatory mechanism has been proposed in which steroid hormones and certain other drugs bind to nuclear receptor proteins followed by transfer to DNA where they are inserted between base pairs.
      
Polymerase chain reaction was used to amplify a 439-bp fragment of a 65,000-kDa (Mr) heat shock protein gene (hsp65) of Mycobacterium.
      
Cloning of an APETALA3 homologous gene (PtAP3) from Populus tomentosa and genetic transformation of its sense and anti-sense con
      
A pair of primers were designed according to published literature on Populus trichocarpa gene (PTD), and PtAP3, an AP3 homologous gene from Populus tomentosa was isolated by PCR using genomic DNA of the male clone of P.
      
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The glutamic dehydrogenase(GDH)-positive mutant, B. subtilis IRC-3-N-1, produced alanine dehydrogenase(ADH)when grown in nutrient medium. The formation of ADH by this mutant was inductive in nature. The inducer for ADH synthesis was not restricted to the substrate of the enzyme. Aspartic acid, threonine, both enantiomorphs of alanine and pyruvate were all effective.During the fractionation of the enzymes, GDH and the induced ADH were coexistent in the same fraction and distributed in almost the same proportions....

The glutamic dehydrogenase(GDH)-positive mutant, B. subtilis IRC-3-N-1, produced alanine dehydrogenase(ADH)when grown in nutrient medium. The formation of ADH by this mutant was inductive in nature. The inducer for ADH synthesis was not restricted to the substrate of the enzyme. Aspartic acid, threonine, both enantiomorphs of alanine and pyruvate were all effective.During the fractionation of the enzymes, GDH and the induced ADH were coexistent in the same fraction and distributed in almost the same proportions. The inductive ADH was found to be indistinguishable from its constitutive form in the wild type in regard to electrophoretic mobility. Since inductive formation of ADH did not cause a corresponding decrease of the constitutive GDH and contrarily, inhibition of ADH synthesis did not bring about any change of the level of the GDH in the mutant cells, the possibility that GDH was converted metabolitically to ADH was ruled out.All the GDH-positive mutants including those obtained by nitrous acid treatment and those of spontaneous origin are capable of inductive synthesis of ADH.In view of the inducibility of ADH synthesis in the GDH-positive mutants and the foregoing data on the immunology of these two enzymes, it was assumed that the genetic transition of ADH to GDH was a result of the mutation of a regulatory gene concerned in the synthesis of ADH.

枯草杆菌B.subtilis IRC-3-N-1,GDH~+变种在营养培养基上生长时能产生ADH。这样合成的ADH是誘导性貭的。誘导物除了酶的底物,L-丙氨酸外,尚有DL-天門冬氨酸,DL-苏氨酸,D-丙氨酸与丙酮酸。将酶进行純化时,GDH与誘导性的ADH始終同时存在于每一步驟中,而且两者的比例不变。誘导性的ADH与野生型细菌的本貭性ADH在电泳移动率上沒有区別。由于ADH誘导形成时,并末观察到变种細胞內GDH的相应減少,同时抑制了ADH的誘导合成,并不引起GDH水平的变化,因之由后者直接轉变成前者的可能性并不存在。所有GDH~+变种,包括自亚硝酸誘发突变或自发突变来源的,都能誘导生成ADH。从以上这些試驗結果,以及以前关于ADH与GDH免疫学試驗的結果,作者們认为枯草杆菌中,由亚硝酸誘发突变而引起ADH轉換成GDH的原因是由于与ADH合成有关的調节基因发生突变的結果。

A slightly modified method of starch gel electrophoresis was used to determine the Hp type of 104 serum samples from a Chinese population. The frequeney of the Hp~1 gene calculated according to Hardy-Weinberg's formula is 0.30, which agrees well with the data reported in the literature.

对Smithies氏原来的淀粉胶电泳作了一些改动,以适应一般实验室条件。本文详细报导了操作过程,并分析了104个健康人的Hp型。计算出的Hp~1频度为0.30此值与文献上的记载甚为接近。

The percentage of body water of the armyworm, Leucania separata Walker, wasfound to fluctuate during its larval development in a regular pattern and to be influencedby the water content of its diet. The body of a newly hatched larva had a water con-tent of about 83.07% and a lipid content of 8.47% (of the dry weight); in the secondinstar its body water content increased to 89% but the lipid content dropped to 2.4%.In the following several instars the water content remained at higher values, which fluc-tuated...

The percentage of body water of the armyworm, Leucania separata Walker, wasfound to fluctuate during its larval development in a regular pattern and to be influencedby the water content of its diet. The body of a newly hatched larva had a water con-tent of about 83.07% and a lipid content of 8.47% (of the dry weight); in the secondinstar its body water content increased to 89% but the lipid content dropped to 2.4%.In the following several instars the water content remained at higher values, which fluc-tuated regularly in the different stages within each instar, being lowest after moulting,increasing during feeding and declining again when it ceased to feed and became readyto moult. The lipid content increased during the larval development until the insectreached the prepupal stage when it dropped. Under laboratory condition, the larva in its life span was found to consume a totalweight of 3 grams of fresh corn leaves (dry weight 0.6 grams). The percentage ofdietary water retention was found to be very high in the first and second instars, about98.9% of the water present in the food, and low in the last instar, the correspondingfigure being 13.67%. This may indicate that there is a sharp difference in the waterrequirement of this insect in the earlier and later stages of its larval development. Corn leaves were used in the study of the influence of dietary water content on thepercentages of food and water utilization, and advantages were taken to compare thereactions of the larvae to the left and right halves of a leaf which were very similar inchemical composition but with their water contents artificially altered by putting themin atmospheres of different relative humidities for a same length of time. Determinationsshowed that when the water contents of the leaf fragments were different, there was nosignificant difference in the percentages of food utilization, but the amounts of food con-sumed were markedly different: the larvae used to consume the leaf with higher watercontent to a significantly greater amount in fresh weight and thus gained a relativelyhigher body weight. Here the water in the leaves was seen to act as a phagostimulant.The adverse effect of leaves with lower water contents on larval growth seemed to bedue to the shortage of this phagostimulant. The larvae feeding on succulent leaves hadhigher water content in their bodies. The evidence of the present work proved that the consumption of a larger amount of food led to the greater accumulation of water derivedfrom the diet rather than the absorption of a larger amount of water from a unit weightof the diet. Thus the relation between the water content of the food plant and theamounts of nutrients absorbed in this insect may be analogous to that in the aphid,where the turgor of the food plant caused by the increase in water content enhancesfood ingestion, but the concentrations of the ingested nutrients decreased (Kennedy,et al., 1959). Dry air was unfavourable for larval growth, especially in younger instars. Gene-rally speaking, as development proceeds, the larva possesses gradually a greater capacityin the regulation of the water content of its body by effective prevention of water eva-poration and production of metabolic water.

粘虫在幼虫的不同发育阶段中,身体的含水量常有明显的变动。这种变化显然可受食物含水量的影响。初孵化幼虫的含水量约在83%左右,到2龄盛食期增高到约为89%,以后变动于90%上下,到末龄成熟时剧烈下降,在化蛹前到达73%左右。在每一龄期中,刚蜕皮而尚未取食时含水量最低,盛食期最高,在蜕皮前又行下降。在试验室条件下以玉米叶饲养的粘虫全幼虫期取食叶片共约3克左右,合干重0.6克;对食物的利用率以鲜重计算为20%左右,以干重计算为24%左右。但不同龄期的食物利用率不同:幼龄的比老龄的高。与此类似的,是不同龄期幼虫对食物中水分的吸收率不同:在一、二龄幼虫中,所吸收的水分占食物鲜重74.9%,相当于食物所含水分的98.9%:而在最后两龄幼虫中,相应数值分别为10.7%和13.6%。故食物中水分对于幼虫的营养作用在幼龄时似乎更为重要。当以其它成分大致相等而含水量不同的玉米叶饲养幼虫时,含水量即使相差不远,也可明显地影响幼虫的生长;此效应在幼龄幼虫中更为显著。随食物所吃下水分的多寡略能影响幼虫对水分的吸收,但差异不显著;此因素虽可影响虫体的含水量,但并不影响幼虫对食物的利用率。食物含水量对幼虫生长的影响,主要是因取食量不同而产...

粘虫在幼虫的不同发育阶段中,身体的含水量常有明显的变动。这种变化显然可受食物含水量的影响。初孵化幼虫的含水量约在83%左右,到2龄盛食期增高到约为89%,以后变动于90%上下,到末龄成熟时剧烈下降,在化蛹前到达73%左右。在每一龄期中,刚蜕皮而尚未取食时含水量最低,盛食期最高,在蜕皮前又行下降。在试验室条件下以玉米叶饲养的粘虫全幼虫期取食叶片共约3克左右,合干重0.6克;对食物的利用率以鲜重计算为20%左右,以干重计算为24%左右。但不同龄期的食物利用率不同:幼龄的比老龄的高。与此类似的,是不同龄期幼虫对食物中水分的吸收率不同:在一、二龄幼虫中,所吸收的水分占食物鲜重74.9%,相当于食物所含水分的98.9%:而在最后两龄幼虫中,相应数值分别为10.7%和13.6%。故食物中水分对于幼虫的营养作用在幼龄时似乎更为重要。当以其它成分大致相等而含水量不同的玉米叶饲养幼虫时,含水量即使相差不远,也可明显地影响幼虫的生长;此效应在幼龄幼虫中更为显著。随食物所吃下水分的多寡略能影响幼虫对水分的吸收,但差异不显著;此因素虽可影响虫体的含水量,但并不影响幼虫对食物的利用率。食物含水量对幼虫生长的影响,主要是因取食量不同而产生的:幼虫对含水较多的叶片取食量较大,因此生长较快。幼虫在?

 
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