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solution temperature
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    The character of seed germination under the effect of the ecological factors of salt solution, temperature, light was discussed. Salt solution promoted seed germination of P. hendersonii as NaCl concentration was less than 0. 6 g/100ml and soil-salt concentration was less than 1.0 g/100ml, the alternate temperature at 25-35℃ was optimum for germination of P. hendersonii, light had no distinct difference to it.
    结果表明,浓度低于0.6g/100mlNaCl和1.0g/100ml土盐能促进大叶白麻的萌发,25—35℃是萌发的适宜温度,光条件对萌发的影响不明显。
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    Methods:Orthogonal design were used to optimize the components of media in shaking-flask,and fed batch fermentation was performed to investigate the factors such as dissolved oxygen,feeding solution,temperature and induction time which would affect the cell growth and the expression of con-IFN.
    方法:在摇瓶实验中,采用正交设计法和均匀设计法确定较佳培养基组分; 使用20 L发酵罐进行补料分批发酵实验,研究影响菌体生长和con-IFN表达的因素(如溶氧、补料、温度和诱导时间)。
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  solution temperature
Moreover, the lower critical solution temperature (LCST) of the graft copolymer in aqueous solution was dependent on its grafting extent and concentration.
      
Copolymers of N,N-diethylacrylamide and N-acryloylphthalimide with a lower critical solution temperature (LCST) were synthesized by radical copolymerization.
      
Dynamic membranes were prepared from poly(N-isopropylacrylamide-co-heptadecyl vinyl ketone) having a lower critical solution temperature in an aqueous solution and being a micelle-forming surfactant.
      
The dependence of the critical concentration of the copolymer micellization on temperature was shown to have an extreme character, with a minimum at the lower critical solution temperature of the copolymer.
      
Other techniques for solving the above problem, namely, an increase in the temperature of the test solution temperature or the use of solvent extraction, bioaccumulation, and unusual analytical procedures are also considered.
      
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Microwave irradiation was applied to maceration of cells of onion root tip meristem after freeze-cracking for scanning electron microscopy. Intermittent microwave irradiation prevented suddenly rise in the solution temperature, from causing damage to tissue specimens during maceration. The maceration time was strikingly shortened from 72 h to 30 min. The intermittent microwave irradiation method, which keeps the temperature low, may be well useful for the sampling procedures including conductive...

Microwave irradiation was applied to maceration of cells of onion root tip meristem after freeze-cracking for scanning electron microscopy. Intermittent microwave irradiation prevented suddenly rise in the solution temperature, from causing damage to tissue specimens during maceration. The maceration time was strikingly shortened from 72 h to 30 min. The intermittent microwave irradiation method, which keeps the temperature low, may be well useful for the sampling procedures including conductive staining. The preparation period of O-D-O method was strikingly shortened.

微波辐射浸软法是一种简单、快速制备高分辨植物扫描电镜样品的方法。经冰冻断裂处理后的样品,利用断续微波辐射浸软,既可阻止浸软溶液温度陡然升高,不使植物结构受到破坏,又可使浸软时间显著缩短,由原来的72小时变为30分钟。因为断续的微波辐射能保持较低的炉内温度,所以它不仅能很好地适用于扫描电镜样品制备过程中的浸软,而且也适用于样品固定、导电染色等。此法大大缩短了O-D-O法的制样周期。

glucose oxidase(GOD) electrode withpolypyrrole(PPy) membrane as the immobiliz-ing mediurn was prepared with a multiple-step process using the″ doping-undoping″property of PPy membrane,The bioelec-trochemical response characteristics were in-vestigated and the kinetic parameters of theenzyme-catalysed reaction were calculated ac-cording to the experimental results. In com-parison with the solubilized GOD ,the elec-trode exhibits good bioelectrochemical charac-teristics and the stabilitv of the enzyme pro-tein...

glucose oxidase(GOD) electrode withpolypyrrole(PPy) membrane as the immobiliz-ing mediurn was prepared with a multiple-step process using the″ doping-undoping″property of PPy membrane,The bioelec-trochemical response characteristics were in-vestigated and the kinetic parameters of theenzyme-catalysed reaction were calculated ac-cording to the experimental results. In com-parison with the solubilized GOD ,the elec-trode exhibits good bioelectrochemical charac-teristics and the stabilitv of the enzyme pro-tein to the solution temperature is promotedsignificantly.

采用分步聚合过程,制备了以聚吡咯膜为载体的葡萄糖氧化酶电极,探讨了其生物电化学响应特性,计算了酶催化反应的有关动力学参数。与溶解态酶相比,该电极表现出良好的生物电化学特性,而且酶蛋白对溶液温度的稳定性有显著提高。

The synthetic thermo\|responsive polymer azidoaniline\|coupled poly( N \| isopropylacrylamide/acrylic acid)(AzPhPIA) was photo\|immobilized on Polystyrene Culture Plates. On the plates, STO cells were cultured for two hours. Under the low critical solution temperature (LCST 21.5℃), detachment experiment showed the cells which were cultured on the thermo\|responsive polymer could not be detached completely as the serum\|free cell culture. For investigating the effects of serum, we chose two serum protein...

The synthetic thermo\|responsive polymer azidoaniline\|coupled poly( N \| isopropylacrylamide/acrylic acid)(AzPhPIA) was photo\|immobilized on Polystyrene Culture Plates. On the plates, STO cells were cultured for two hours. Under the low critical solution temperature (LCST 21.5℃), detachment experiment showed the cells which were cultured on the thermo\|responsive polymer could not be detached completely as the serum\|free cell culture. For investigating the effects of serum, we chose two serum protein (albumin and fibronectin) for cell attachment and detachment.The results showed that fibronectin was coated all the surface of plate, but albumin was pattern\|coated on the plate and liked to coated on the surface of polystyrene. It was fibronectin which increased cell attachment so that cells could not be detached, and was due to hydrophilic of thermo\|responsive polymer AzPhPIA not the attachment influence of albumin that cells were detached under the LCST.Surface analysis demonstrated that coated protein would not affect the wettability of AzPhPIA.

探讨血清对细胞剥离的影响.将温度应答性高分子聚( N异丙基丙烯酰胺/ 丙烯酸)的叠氮苯胺的衍生物(AzPhPIA) 条纹状地光固定在组织培养聚苯乙烯(PSt) 基板上,并对鼠成纤维细胞(STO) 血清培养2h .温度低于最低临界温度(LCST) ,粘附在AzPhPIAPSt 上的细胞不能很好地剥离.选用血清中两种典型蛋白质白蛋白和纤维粘连蛋白,模拟血清进行细胞粘附和剥离实验.结果表明,纤维粘连蛋白在基板上全面而均匀地附着,从而加强了细胞与基板表面的粘附性,使细胞在低温时不易剥离;白蛋白在基板上的附着是条纹状的,AzPhPIAPSt 表面附着白蛋白少,这说明是由于温度应答性高分子表面亲水性提高,高分子链伸展而不是白蛋白的阻害作用引起细胞的脱落.另外,表面分析结果表明,蛋白质的吸收并不影响AzPhPIAPSt 材料表面的可湿性.

 
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