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genetic markers
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  遗传标记
     The mutants with two genetic markers, TK15-94-33(a lys- phe-)and TK63-41-72(a ade- leu-), were selected after strains TK15-94 and TK63-41 were treated by EMS.
     EMS诱变后得到带有两个遗传标记的单倍体TK15-94-33(a lys~- phe~-)和TK63,41-72(a ade~- leu~-)。
短句来源
     Conclusion: D 16 S 539 ,D 7S 820 and D 13 S 317 loci may be very useful genetic markers to study population genetics and forensic genetics .
     结论 :D16S539、D7S82 0 、D13S317基因座是进行人类群体遗传学和法医遗传学研究十分有用的遗传标记
短句来源
     This study suggested that there was an aggregative phenomenon in some hypertensive. The genotypes of np152C, np182T, np247A, np16187T, np16189C, np16264T, np16270T and np16311C may be potential genetic markers for susceptibility to hypertension.
     研究提示部分高血压病患者有群聚现象,基因型np152C、np182T、np247A、np16187T、np16189C、np16264T、np16270T和np16311C可能是此聚类族高血压患者的易感遗传标记
短句来源
     METHODS Thirty three strains of PAE were isolated from hospitalized patients in ICU,and the class 1 integron genetic markers(including intⅠ1 and qacE△1-sul1) and 22 kinds of the drug-resistant genes were analyzed by PCR.
     方法对ICU分离33株PAE,采用PCR方法检测Ⅰ类整合子遗传标记(intⅠ1和qacE△1-sul1基因)及22种抗菌药物耐药相关基因。
短句来源
     Establishment of Wg3h-neo Cell Line with Two Genetic Markers
     双遗传标记细胞系Wg3h-neo的建立
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  遗传标志
     Analysis of 12 genetic markers in 5 schizophrenia families
     5个精神分裂症家系的12个遗传标志分析
短句来源
     Methods: Patients who fulfilled the criteria for schizophrenia according to DSM - Ⅲ and their siblings from 5 families were selected. 12 genetic markers of MAOACA, MAOBTG,MAOBI2,DXS7, D6S296,D6S274,D6S470,D6S260,SCAK D9S175, DAT1 和APOE were tested by PCR - RFLP and Amp - FLP and the transmission disequilibrium test (TDT) analyzed.
     方法按照美国精神障碍诊断与统计手册第3版修订本的精神分裂症诊断标准,选择5个同胞对精神分裂症家系,采用PCR-RFLP、小卫星和微卫星DNA的Amp- FLP技术,观察12个遗传标志MAOACA、MAOBTG、MAOBI2、DXS7、D6S296、D6S274、D6S470、D6S260、 SCA1、D9S175、DAT1和APOE的分布。
短句来源
     Methods The microsatellite polymorphism genetic markers of glucokinase (GCK,MODY2) gene with highest mutation frequency and hepatocyte nuclear factor-lα ( HNF-lα, M0DY3) gene were selected to perform the classical linkage analysis in two Chinese N1DDM/M0DY families.
     方法 选择MODY基因中突变率最高的葡萄糖激酶(GCK,MODY2)和肝细胞核因子1α(HNF-1α,MODY3)基因的微卫星多态遗传标志在2个包含临床MODY患者的中国人2型糖尿病家系中进行连锁分析,对HNF-1α基因进行全基因外显子的突变筛查-DNA序列直接测定以明确具体的核苷酸突变和所编码的氨基酸的改变。
短句来源
     Conclusions HLA-A*02 alleles possess high heterogeneity and genetic diversity in Chinese Han population with significantly different distributions in the two populations. HLA-A**020101, A*0203 and A*0207 may serve as the genetic markers for dividing Chinese Han individuals into southerners and northerners in anthropological studies.
     结论HLA-A*02等位基因在中国南、北汉族人群中的分布呈现高度杂合和遗传多样性并具有显著性差异,HLA-A*020101、A*0203和A*0207可作为人类学研究中区分中国南北汉族人群的遗传标志
短句来源
     The current development of chicken genetic markers and the application prospect of breeding were summarized in the paper.
     综述了鸡抗马立克氏病遗传标志的选择进展以及在抗马立克氏病育种中的应用前景
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  “genetic markers”译为未确定词的双语例句
     ConclusionsD5S436 and D5S658 were good genetic markers.
     结论D5S436和D5S658是两个较好的遗传连锁标记;
短句来源
     Objective To explore the genetin polymorphism at D19S253 and D12S391 and D7S820 loci in Han Nationality and to evaluate the validation of these 3 loci as genetic markers for forensic haemogenetics in Henan Province.
     目的探讨D19S253、D12S391、D7S820基因座在河南汉族人群的法医学应用价值。
短句来源
     MethodsWe chose 9 X-STR(DXS6804,DXS7133,DXS101,DXS6789,DXS6799,DXS7423,HPRTB,DXS8378,DXS7132) as genetic markers from 99 irrelative individules to determine the genetic diversity of Ewenki in Inner Mongolian.
     方法:选择9个X-STR(DXS6804,DXS7133,DXS101,DXS6789,DXS6799,DXS7423,HPRTB,DXS8378,DXS7132),分析其多态性分布及与其他群体间的遗传距离和聚类关系。
短句来源
     Every linkage group has 6-32 genetic markers,average genetic distance is 9.72-19.19 cM,and the length of linkage group is 85.3-496.1 cM.
     每个连锁群的标记数6~32个,平均图距9.72~19.19cM,连锁群长度85.3~469.1cM。
短句来源
     The SNPs of hsp60 gene rs1116734,rs3749095,rs1050347,rs8539 are very common in Chinese Han people and might be used for candidate genetic markers of hsp60 gene.
     结论中国汉族人群hsp60的SNPs分布有别于其他人种,rs1116734、rs3749095、rs1050347、rs8539是hsp60基因在中国汉族人群中较常见的SNPs,可为Hsp60蛋白功能研究及hsp60基因与疾病关联研究候选SNPs的选择提供科学依据。
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  genetic markers
Allelic frequencies of the revealed loci are proposed as genetic markers for monitoring L.
      
earthworms collected in different parts of the Moscow and Kiev regions was studied by means of electrophoresis in polyacrylamide gel, using the loci encoding unspecific esterases and generic proteins as biochemical-genetic markers.
      
The North Eurasian population (41 local populations of 21 ethnic groups) was tested for genetic diversity with numerous genetic markers, including Y-chromosomal haplotypes, autosomal microsatellites, and polymorphic Alu insertions.
      
Many known mechanisms of drug resistance in microorganisms have genetic markers, which are specific genomic changes, mostly single-nucleotide polymorphisms (SNPs).
      
Dedifferentiation and proliferation of cells and formation of progenitor multipotent cells, which are a source of retina regeneration in adult newts, were characterized using cell, molecular, and genetic markers.
      
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Intact maize (Zea mays) ovaries were excised from unpollinated ears of field-grown plants and after cutting into 1/3 level were placed on modified White medium. Application of sterilized pollen to the naked ovules resulted in seeds formation of 0.42% of all ovules. A total number of 14 kernels were obtained in 9 sets of experiments. The plant of F1 have genetic marker of purple colour in the stalk and the ear's husks. The chromosome number of its root cells: 2n = 20. All these proved that the plant is...

Intact maize (Zea mays) ovaries were excised from unpollinated ears of field-grown plants and after cutting into 1/3 level were placed on modified White medium. Application of sterilized pollen to the naked ovules resulted in seeds formation of 0.42% of all ovules. A total number of 14 kernels were obtained in 9 sets of experiments. The plant of F1 have genetic marker of purple colour in the stalk and the ear's husks. The chromosome number of its root cells: 2n = 20. All these proved that the plant is a hybrid originating from test-tube fertilization of ovules.

从未授粉的玉米植株的雌穗上,切除子房上部的三分之一,获得裸露的胚珠。在切口处授以经过消毒的玉米花粉,获得了14粒由胚珠发育的幼嫩种子。在授粉后20—22天幼嫩种子的胚已经成熟,并在果穗块上萌发和生长,现已获得两个植株。其中一株植株上有明显的父本标记的紫色素性状及类似父本的旗叶。根尖细胞染色体2n=20,F_2代有分离,因此证明是胚珠离体受精而来的杂种植株。另一株植株形态、色素、旗叶均与母本相似,染色体2n=20,生长健壮,雄穗很早抽出,但在抽雄后49天才长出弱小的雌穗,未获得种子。

The present study is devoted to the identification and isolation of translocations in the mosquito Culex pipiens pollens Coq. Virgin female mosquitoes carrying genetic markers, recessive red eye (r) and black head (bh) were mated with wild males pretreated with X-ray radiation and the F1 obtained were individually backcrossed with red-eyed and black-headed ones. Among the group of individuals tested with reciprocal cross translocation strains were isolated and maintained. Two of them are M-linked and the...

The present study is devoted to the identification and isolation of translocations in the mosquito Culex pipiens pollens Coq. Virgin female mosquitoes carrying genetic markers, recessive red eye (r) and black head (bh) were mated with wild males pretreated with X-ray radiation and the F1 obtained were individually backcrossed with red-eyed and black-headed ones. Among the group of individuals tested with reciprocal cross translocation strains were isolated and maintained. Two of them are M-linked and the two others m-link-ed. The translocation heterozygote strains showed different degrees of sterility, ranging from 53.1 to 71.5 per cent. The observations indicated that the sterility was due to lethality of the fertilized eggs.

相互易位能使生殖细胞分裂失常而导致部份不育,故可用来作为防治害虫的手段。我们用1000到8000伦琴的X射线,照射的羽化24到48小时的淡色库蚊(Culex pipiens pallens Coq.)的野生型雄蚊,诱发了性染色体和常染色体之间的相互易位。为鉴定并分离出这种相互易位,用未经交配的具有红眼(r)和黑头(bh)两种突变性状为标记的雌蚊与受照射的野生型雄蚊进行单对交配。F_1表现为野生型,BC_1由r与bh两种性状所表现的拟连锁r-bh及分离比例上的反常,分别从4000、5000、6000和8000伦琴的四个照射组中,各分离出一种相互易位杂合子。其中由5000和8000伦琴诱发所得为雄性连锁相互易位杂合子T~M,而由4000和6000伦琴诱发所得为雌性连锁相互易位杂合子T~m。都已育成稳定的半不育品系。四个品系的不育性为53.10%—71.50%。经详细观察,发现不育性的主要原因是受精卵的致死所造成。

Restriction endonuclease EcoR1and BamH 1 were used to producefragments pBR 322 C (375 bp) andpBR 322 B (3987 bp) from pBR 322and to produce λF_2A (65.6—71.3%of λ DNA,2967 bp) and λF_2B (71.3—81% of λDNA,4559 bp) fromEcoR1 restriction fragment λF_2(65.6—81% of λDNA) of λcI_(857)S_7DNA.By recombining pBR 322 B andλF_2B in vitro,a new plasmid calledpCB 2 carrying λ promoters and struc-tural genes cI and cro was constructed.The desired strain with pCB 2 wasselected from 338 transformants forits Ap~rTc~s and for...

Restriction endonuclease EcoR1and BamH 1 were used to producefragments pBR 322 C (375 bp) andpBR 322 B (3987 bp) from pBR 322and to produce λF_2A (65.6—71.3%of λ DNA,2967 bp) and λF_2B (71.3—81% of λDNA,4559 bp) fromEcoR1 restriction fragment λF_2(65.6—81% of λDNA) of λcI_(857)S_7DNA.By recombining pBR 322 B andλF_2B in vitro,a new plasmid calledpCB 2 carrying λ promoters and struc-tural genes cI and cro was constructed.The desired strain with pCB 2 wasselected from 338 transformants forits Ap~rTc~s and for its immunity to λ infection.pCB 2 was characterized by:1.its genetic marker Ap~rTc~s,because BamH1 site is right in theTc region of pBR 322,so as to renderit Tc~s.2.transforming C 600 to be im-mune to λcIs857-S7 infection,since λF_2B contains immune regionof this λDNA,with cI and cro geneswhich are expressed under the direc-tion of the λpromoters to producerepressor and cro protein respectivelyand thus plaque formation is preven-ted.This shows that the promotersin our pCB 2 are working and thatcI or/and cro gene in λDNA fragmentis expressed in E.Coli.Moreover,the gene product which is responsiblefor the immunity to λinfection isthermostable.3.having the length of 2.66±0.33μ and MW of 5.51±0.68×10~6dalton (calculated MW=5.41×10~6dalton) estimated by electron mi-croscope and gel electrophoresis afterdigestion with either EcoR 1 orBamH 1.4.giving rise to 2 molecules ofdifferent MW.after digestion withboth EcoR1 and BamH 1.The largeone corresponds to λF_2B while thesmall one corresponds to pBR 322 B.This excludes pCB 2 from beingpBR 322 B dimer or λF_2B dimer whichshould give rise to 2 identical mole-cules (ie.pBR 322 B or λF_2B re-spectively) upon digestion with thesame two enzymes.Further more,the bacterial harboring pBR322Bdimer should show no immunity tothe infection with phage λ,whileλF_2B dimer should be Ap~sTc~s in-stead of Ap~rTc~s.5.its transforming activity.Thefrequency of transformation is 2.1×10~6CFU/μg DNA.6.giving a heteroduplex afterdenaturation and renaturation of λF_2and pCB 2 together.The lengthsmeasured for the single stranded partsand double stranded part agree wellwith our original design.From the above characteristics ofpCB 2,we come to the conclusion thatpCB 2 we constructed is a new plas-mid with cI and/or cro gene expressedunder the direction of λ promoters.

我们用EcoR1及BamH1两种限制性核酸内切酶来酶解质环pBR322成为pB-R322C(375bp)及pBR322B(3987bp),并酶解λcI857S_7DNA 的EcoR1限制片段λF_2(λDNA 的65.5—81%片段)成为λF_2A(λDNA 的65.6—71.3%片段,2679bp)及λF_2B(λDNA 的71.3—81%片段,4559bp)。再用T_4-DNA 连接酶将pBR322B 及λF_2B重组成为一个新质环,叫做pCB_2,其上具有cI 基因,cro 基因及启动基因,这是从随机挑取的338个菌落的第48号菌所携带的新质环。pCB_2的性质是:1.它赋予转化子以单抗药性(Ap~r),2.它赋予转化子对λcI857S_7感染的免疫性,由此可见,我们接上去的λ启动基因在发挥作用,cI 基因或/和cro 基因得以表达。第48号菌经过42℃处理后,其免疫能力仍不低于处理前的。3.经EcoR1或BamH1一种酶水解后,用电镜及凝胶电泳检查,测得pCB_2的分子长度为2.66±0.33μm,分子量为5.51±0.68×10~6d(计算值为5.41×10~6d,8546bp)。4.经过EcoR1...

我们用EcoR1及BamH1两种限制性核酸内切酶来酶解质环pBR322成为pB-R322C(375bp)及pBR322B(3987bp),并酶解λcI857S_7DNA 的EcoR1限制片段λF_2(λDNA 的65.5—81%片段)成为λF_2A(λDNA 的65.6—71.3%片段,2679bp)及λF_2B(λDNA 的71.3—81%片段,4559bp)。再用T_4-DNA 连接酶将pBR322B 及λF_2B重组成为一个新质环,叫做pCB_2,其上具有cI 基因,cro 基因及启动基因,这是从随机挑取的338个菌落的第48号菌所携带的新质环。pCB_2的性质是:1.它赋予转化子以单抗药性(Ap~r),2.它赋予转化子对λcI857S_7感染的免疫性,由此可见,我们接上去的λ启动基因在发挥作用,cI 基因或/和cro 基因得以表达。第48号菌经过42℃处理后,其免疫能力仍不低于处理前的。3.经EcoR1或BamH1一种酶水解后,用电镜及凝胶电泳检查,测得pCB_2的分子长度为2.66±0.33μm,分子量为5.51±0.68×10~6d(计算值为5.41×10~6d,8546bp)。4.经过EcoR1及BamH1二种酶水解后,分解为二种分子,一大一小。在凝胶电泳中,其分子量大的与λF_2B 相当,小的与pBR322B 相当。5.pCB_2具有生物活性,其转化频率为2.1×10~6CFU/μgDNA。6.用λF_2与经历EcoR1切割成线状的pCB_2在一起进行变性后复性,在电镜下观察异源双链图形,其长度与建造时的设计相符。从以上结果我们得出的结论是:我们建造的PCB_2是一个新质环,其中的CI 基因及/或cro 基因,能在λ启动基因指导下,在大肠杆菌中表达。

 
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