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submerged culture     
相关语句
  深层培养
     A 1. SL Outer Airlift Loop Reactor(OALR) is used for the submerged culture of B. edLilis The optimum ventilating control is: 0~ 24h 0. 3vvm;
     以 1.5L外循环气升式反应器(OALR)进行美味牛肝菌(B. edulis)的深层培养试验,其最适通风量为 0~24h, 0 3vvm;
短句来源
     The optical density (OD600 nm) of cells of submerged culture in NaAc-Malate medium and sodium lactate medium were 0.7 and 1.7 respectively within 25 hours.
     在乙酸钠-苹果酸培养基和乳酸钠培养基中深层培养25h的细胞密度分别为0.7和1.7OD600nm。
短句来源
     By submerged culture experiment of Pleurotus ferulae,the authors found the optimum liquid medium. Composition of the medium consists of 5.0% cornmeal,{1.0%} sucrose, 0.3% KH-2PO-4, 0.15% MgSO-4·7H-2O, 0.2% yeast paste,{0.1%} α-starch enzyme and VB-1 10mg/L.
     通过对白灵菇进行深层培养工艺的研究 ,发现白灵菇菌丝体生长的液体培养基的最适配方为 :玉米粉 5 % ,蔗糖 1 % ,KH2 PO40 .3 % ,MgSO4·7H2 O 0 .1 5 % ,VB11 0mg/L ,α 淀粉酶 0 .1 %。
短句来源
     To sift out the rapid-growth Ganoderma lucidum (GL) strain in submerged culture, the product and optimal culture conditions of four kinds of GL strain (Japan strain 1,Japan strain 7, South Korea strain and strain G4) were investigated.
     为扩大灵芝药源,筛选生长快,适于深层培养的优良灵芝菌株,选用日本灵芝7号、日本灵芝1号、韩灵芝及G4灵芝菌株为材料,比较了不同灵芝菌株菌丝体的生产能力及其液体深层培养条件,结果表明:日本灵芝7号菌株生长最快,菌丝体干重为15.44g/L,G4菌株次之;
短句来源
     A 1.5 L outer airlift loop reactor(OALR) was used for the submerged culture of Ganoderma lucidum.
     用1.5 L外循环气升式反应器(OALR)对赤芝菌进行深层培养实验。
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  液体培养
     It was proved in selecting optimum medium for submerged culture of Morchella conica that the proper submerged culture conditions for PYG medium were 25℃, 100r/min, pH7.5, and 100mL liquid medium put in 500mL bottle with 10 glassballs, and that the constituents of shaking medium selected by orthogonal test were 50% malt extract, 1.5% com meal, 0.5% yeast extract plus 1.5% sucrose.
     羊肚菌液体摇瓶培养条件以及适用培养基的筛选研究表明,PYG培养基(含0.1%酵母提取物的PDA)合适的液体培养条件是:25℃,100r/min,装量100mL/500mL三角瓶,加10粒玻璃珠,pH7.5。
短句来源
     Study on enriched germanium of Ganoderma lucidum by submerged culture
     灵芝液体培养富锗的研究
短句来源
     Selection of the Optimum Conditions for Submerged Culture of Taishan Cordyceps sp.(C-2)
     泰山虫草C-2最优液体培养条件筛选
短句来源
     And the results of L_(16)(4~5)orthogonal test of O. radicata in submerged culture proved that the broth had the strongest antagonism to the mycelial growth of B. maydis, when the submerged-culture medium was composed of corn meal 4%, sucrose 3%, soybean meal 0.5% and peptone 0.2% with the initial pH being 6.0. Furthermore, there was a significant positive correlation between the mycelial biomass of O.
     L16 (4 5)正交试验结果表明 ,以玉米粉 4 % ,蔗糖 3% ,黄豆粉 0 .5 % ,蛋白胨 0 .2 %为培养基 ,初始pH6时 ,液体培养长根金钱菌发酵液对玉米小斑病菌菌丝生长的抑制作用最强 ,抑制率与长根金钱菌的生物量呈显著正相关。
短句来源
     A Preliminary Study on Submerged Culture of Morchella conica
     羊肚菌液体培养研究初报
短句来源
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  深层发酵
     L-Malic Acid Fermentation by Submerged Culture Method in 100L Fermentor
     100L罐中深层发酵生产L-苹果酸的研究
短句来源
     Studies on the Production of Xylanase for Submerged Culture by Aspergillus Clavatus GM-69
     棒曲霉GM-69深层发酵生产木聚糖酶的研究
短句来源
     Study on Optimization of Submerged Culture of Pleurotus Nebrodensis by Regressive Model
     回归模型优化白灵菇深层发酵工艺
短句来源
     STUDY ON MEDIUM OF SUBMERGED CULTURE FOR GRIFOLA FRONDOSA
     灰树花深层发酵培养基的研究
短句来源
     Production of monacolin K in submerged culture by citrinin-free Monascus species
     不产桔霉素的红曲霉菌种深层发酵生产莫纳可林K
短句来源
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  沉没培养
     Antibiotic 8653 is the secondary metabolite produced by submerged culture of mic-romonospora roseotus n.
     抗生素8653是近玫瑰小单孢菌新种8653沉没培养产生的代谢物。 它的理化性质和生物学性质与已报道的蔷薇霉素(Rosamicin)及抗生素104-Ⅰ基本一致。
短句来源

 

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      submerged culture
    Enzyme activity in Penicillium funiculosum mutants was studied in submerged culture.
          
    ostreatus growing on solid agar medium and in a submerged culture with a synthetic medium.
          
    The time course of lectin production in culture liquid of the basidial fungus Lentinus edodes strain F-249 in different media under submerged culture conditions was studied.
          
    Twenty strains of the wood-degrading fungi from the genus Trametes Fr., capable of synthesizing laccases, were screened according to the changes in the oxidase activity in a submerged culture.
          
    The absence of correlation between the oxidase activity in a submerged culture and the size of colored zone on agar media (Bavendamm reaction) was demonstrated.
          
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    1. α-amylase, saccharifying enzyme, maltase and limit dextrinase have been tested in this research work. According to the results of more than 10 experiments, the following conclusions may be made: (a)Although Asp. oryzae, No. 7 possesses greatest liquifying power, the aleohol yield by using this mold as saccharifying agent is lowest among all the molds tested. It is obvious that, there is no direct relationship between α-amylase value and alcohol yield. (b) Maltase value of Asp. oryzae No.7 is much lower than...

    1. α-amylase, saccharifying enzyme, maltase and limit dextrinase have been tested in this research work. According to the results of more than 10 experiments, the following conclusions may be made: (a)Although Asp. oryzae, No. 7 possesses greatest liquifying power, the aleohol yield by using this mold as saccharifying agent is lowest among all the molds tested. It is obvious that, there is no direct relationship between α-amylase value and alcohol yield. (b) Maltase value of Asp. oryzae No.7 is much lower than that of other molds, for example, mal-tase value of Asp. niger, NRRL 330 is 7.24,while that of Asp. oryzae, No.7 is only 0.06. The alcohol yield of the latter is about 20% less than that of the former. in other words, the higher the maltase value, the higher the alcohol yield. This conclusion is obviously in agreement with previous investiga- tions. When the saccharifying value is high, the limit dextrinase value is also high. But the inter-relationship between limit dextrinase and alcohol yield requires further investigation. 2. Concerning the selection of mold suitable for submerged culture, Asp. niger, No. 2 is the best one, according to the results of 1st and 2nd period of our experiments; while Asp. niger, NRRL 330 isnext to Asp. niger No. 2. 3. Concerning the selection of culture medium, 3 kinds of media: (1) potato powder 8%, NaNO_2 0.3%, HCl (37%) 0.1%; (2) maize 5%, rice bran 1%, wheat bran 1.5%; (3) distillers solubles 3.5% maize 5%,(pH 5.0) have been adopted. Among these media, No. 2 is the best, No. 1 is next and No.3 is not suitable for Asp. niger, No. 2.

    酒精的用途很大,无论在国防工业上、医药卫生上以及其他有关工业上都需要大量酒精。另一方面,我国土法制酒,淀粉利用率很低,因此,浪费大量粮食。最近中央倡议“白酒酒精化”,就是用酒精配制白酒,因为新法造酒的淀粉利用率较高,可以节约很多粮食。此后酒精工业对国民经济的关系更大,我们科学工作者的任务也更繁重。

    As the result of a series of experiments, the following conclusions can be made: 1. The optimum pH of saccharifying enzyme and maltase of the submerged culture of Aspergillusniger, NRRL 330 is between 3 and 5 at the temperature below 60℃. At any value of pH, the optimumtemp. is 60℃. At higher temp. the sensitivity of these amylases toward pH will be greater. Beyondthe optimum pH range, the saccharifying enzyme and maltase are much more stable at the acid sidethan at the alkaline side. When pH is over 6,...

    As the result of a series of experiments, the following conclusions can be made: 1. The optimum pH of saccharifying enzyme and maltase of the submerged culture of Aspergillusniger, NRRL 330 is between 3 and 5 at the temperature below 60℃. At any value of pH, the optimumtemp. is 60℃. At higher temp. the sensitivity of these amylases toward pH will be greater. Beyondthe optimum pH range, the saccharifying enzyme and maltase are much more stable at the acid sidethan at the alkaline side. When pH is over 6, the activities of the saccharifying enzyme and maltasedecrease greatly; at pH 7, as the temp. increasing to 70℃, they almost lose their activities. 2. The optimum pH of the dextrinizing enzyme of the submerged culture of Asp. niger is alsobetween 3 and 5. The optimum temp. is 60-70℃. At the optimum pH, the dextrinizing enzyme increasesin direct proportion with the temp. Same as saccharifying enzyme its sensitivity toward pH increaseswhen the temp. is higher. 3. From the results of the experiments ncerning the thermal resistance of saccharifying enzymeof submerged-culture of Asp. niger and A. oryzae, we know that the thermal resistance of the formeris much stronger than that of the latter. When treated at 50℃ for 3 hrs, the saccharifying activity of A. niger lost only by 10%, whilethat of A. oryzae by 70%. When Asp. niger, NRRL 330 is treated at 60℃ for 1 hr., only 35% of thesaccharifying activity is lost; while at the same condition, 80% of the saccharifying activity of A. oryzaewill be lost. In the manufacture of alcohol, amylase which acquires stronger thermal resistance always givebetter results. If the thermal resistance of amylase is strong, the saccharifying temp. of the mash may be higher.Concerning this point the following advantages may be mentioned: (1) At higher temp. the decrease in the viscosity of the mash and the increase in the rate ofsaccharification are both favorable for the fermentation process. (2) Prevention of bacterial contamination at higher temp. of saccharification results higheralcohol yield. (3) Having acquired greater thermal resistance, the saccharifying enzyme during and after thesaccharification process will be negligibly destroyed, which in turn will not effect much of its effectiveness. 4. By using Kitahara's method of fractional quantitative analysis to decide the type of amylasescontaining in the submerged culture of A. niger and A. oryzae, the following results are obtained: At the value of pH 2.5, the saccharifying activity of A. oryzae is entirely lost, so the amylase ofA. oryzae may belong to α-type. Although the pH is lowered to 2.5, the liquifying power of A. niger, NRRL 330 is only slightlyeffected. In this case, A. niger, NRRL 330, perhaps contains an acid fast liquifying enzyme, which, onthe contrary, being destroyed at pH 7 (55℃), is different from the ordinary α-amylase. Moreover, the saccharifying enzyme of A. niger is only slightly effected at pH 7 (55℃). It isobvious that this amylase is not the same as the ordinary β-amylase. At pH7 (55℃ 15 min), ere is no great influence on maltase activity of A. niger. But this resultdiffers from Kitahara's report appreciably. From the above experiments, we can see that the acid resisting power of amylases of A. nigeris much stronger than that of A. oryzae.

    由淀粉质原料制造酒精以液体麯为糖化剂时,对于液体麯所含各种淀发酶的特性,必须彻底明了,方能确定糖化所需的最适温度、时间与pH值,否则淀粉酶在制造过程中受到损害,结果将大大影响淀粉利用率。本试验中,黑麯霉以Asp.niger,NRRL 330为菌种,黄麯霉以Asp.oryzae,No.7为菌种。报告内容分为:Ⅰ温度、pH对于黑麯霉的液麯糖化酶的影响。Ⅱ温度、pH对于黑麯霉的液麯α淀粉酶的影响。Ⅲ温度、pH对于黑麯霉的液麯麦芽糖酶的影响。Ⅳ黑麯霉的液麯麦芽糖酶的最适温度。Ⅴ黑麯霉的液麯黄麯霉的液麯淀粉酶的耐热性比较试验,Ⅵ黑麯霉、黄麯霉的液麯淀粉酶类型的研究。

    Glutamic acid has been conventionally used as a nitrogen source for the medium in studying ergometrine production by ergot organisms in submerged culture. The high price of glutamic acid, however, is a serious disadvantage for large scale production of ergot alkaloids. Thus we attempted to select several cheaper and easily obtainable natural subatances for the substitution of glutamic acid.It was shown through our experiments that crucian carp (Carassius suratus) meal and silver carp (Hypaphthalmichths...

    Glutamic acid has been conventionally used as a nitrogen source for the medium in studying ergometrine production by ergot organisms in submerged culture. The high price of glutamic acid, however, is a serious disadvantage for large scale production of ergot alkaloids. Thus we attempted to select several cheaper and easily obtainable natural subatances for the substitution of glutamic acid.It was shown through our experiments that crucian carp (Carassius suratus) meal and silver carp (Hypaphthalmichths molitrix) meal were most effective, resulting in pronounced increase of ergot alkaloid production as compared with glutamic acid.Different concentration of crucian carp meal were tested. It was found that the 4% meal gave best results, with total alkaloid and ergometrine production of 0.0185% and 0.0115% respestively.Using crucian carp meal as the nitrogen source in 200-liter submerged fermentors, the ergot orgamism (Strain Ge-3-45) started to produce ergot alkaloid on the 4th day. The amount of the total alkaloid and ergometrine reached maximum, 0.2034% and 0.01781% in filtrate(0.1609% and 0.0495% in mycelium) respectively, after about 11 days fermentation.It may be concluded that crucian carp meal is a Letter nitrogen source than glutamic acid for the production of ergot alkaloid in submerged culture, not only for its greater enchancement of the production of ergometrine but also for its availability and inexpensiveness.

    在以前工作的基础上,我们进一步采用鲫鱼粉代替谷氨酸生产麦角新碱取得了比较好的效果。 通过试验证明,鲫鱼粉不仅可代替谷氨酸作为氮源,并能促进麦角新碱的形成,其麦角新碱含量比对照高近一倍,且其来源容易,价格低廉。 麦角菌在200l发酵罐内培养,从第四天开始产生麦角碱,发酵至11天麦角碱含量已达最高峰。在发酵过滤液中麦角总生物碱及麦角新碱含量分别为0.0203%及0.0178%,而在菌丝体中分别达到0.1609%及0.0495%。

     
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