The optical density (OD600 nm) of cells of submerged culture in NaAc-Malate medium and sodium lactate medium were 0.7 and 1.7 respectively within 25 hours.
By submerged culture experiment of Pleurotus ferulae,the authors found the optimum liquid medium. Composition of the medium consists of 5.0% cornmeal,{1.0%} sucrose, 0.3% KH-2PO-4, 0.15% MgSO-4·7H-2O, 0.2% yeast paste,{0.1%} α-starch enzyme and VB-1 10mg/L.
To sift out the rapid-growth Ganoderma lucidum (GL) strain in submerged culture, the product and optimal culture conditions of four kinds of GL strain (Japan strain 1,Japan strain 7, South Korea strain and strain G4) were investigated.
It was proved in selecting optimum medium for submerged culture of Morchella conica that the proper submerged culture conditions for PYG medium were 25℃, 100r/min, pH7.5, and 100mL liquid medium put in 500mL bottle with 10 glassballs, and that the constituents of shaking medium selected by orthogonal test were 50% malt extract, 1.5% com meal, 0.5% yeast extract plus 1.5% sucrose.
And the results of L_(16)(4~5)orthogonal test of O. radicata in submerged culture proved that the broth had the strongest antagonism to the mycelial growth of B. maydis, when the submerged-culture medium was composed of corn meal 4%, sucrose 3%, soybean meal 0.5% and peptone 0.2% with the initial pH being 6.0. Furthermore, there was a significant positive correlation between the mycelial biomass of O.
The time course of lectin production in culture liquid of the basidial fungus Lentinus edodes strain F-249 in different media under submerged culture conditions was studied.
Twenty strains of the wood-degrading fungi from the genus Trametes Fr., capable of synthesizing laccases, were screened according to the changes in the oxidase activity in a submerged culture.
The absence of correlation between the oxidase activity in a submerged culture and the size of colored zone on agar media (Bavendamm reaction) was demonstrated.
1. α-amylase, saccharifying enzyme, maltase and limit dextrinase have been tested in this research work. According to the results of more than 10 experiments, the following conclusions may be made: (a)Although Asp. oryzae, No. 7 possesses greatest liquifying power, the aleohol yield by using this mold as saccharifying agent is lowest among all the molds tested. It is obvious that, there is no direct relationship between α-amylase value and alcohol yield. (b) Maltase value of Asp. oryzae No.7 is much lower t...
As the result of a series of experiments, the following conclusions can be made: 1. The optimum pH of saccharifying enzyme and maltase of the submerged culture of Aspergillusniger, NRRL 330 is between 3 and 5 at the temperature below 60℃. At any value of pH, the optimumtemp. is 60℃. At higher temp. the sensitivity of these amylases toward pH will be greater. Beyondthe optimum pH range, the saccharifying enzyme and maltase are much more stable at the acid sidethan at the alkaline side. When pH is...
Glutamic acid has been conventionally used as a nitrogen source for the medium in studying ergometrine production by ergot organisms in submerged culture. The high price of glutamic acid, however, is a serious disadvantage for large scale production of ergot alkaloids. Thus we attempted to select several cheaper and easily obtainable natural subatances for the substitution of glutamic acid.It was shown through our experiments that crucian carp (Carassius suratus) meal and silver carp (Hypaphthal...