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cell growth     
相关语句
  细胞生长
     Research on Platelet-derived Endothelial Cell Growth Factor in Bladder Transitional Cell Carcinoma
     膀胱移行细胞癌血小板衍化内皮细胞生长因子研究
短句来源
     Studies on T Cell Growth Factor Effect of Immuno-modulating Agents on T Cell Growth Factor Production
     T细胞生长因子的研究免疫调变剂对T细胞生长因子产生的影响
短句来源
     STUDIES ON PHYSIOLOGICAL AND BIOCHEMICAL CHARACTER IN SUSPENSION CULTURE OF BERBERIS JULIANAE SCHNEID CELLS Ⅰ.Relationship between cell growth and changes in the conductivity and peroxidase activity of cultured medium
     九连小蘖悬浮培养细胞生理生化研究 Ⅰ.细胞生长与培养液的电导率和过氧化物酶活性的变化
短句来源
     The influence of B Cell Growth Factor in Activity Superna-tant on the Growth of Human-Human Hybridomas
     B细胞生长因子活性上清对人—人杂交瘤细胞生长影响的研究
短句来源
     PARAMETER ESTIMATION FOR THE CELL GROWTH KINETIC MODELS
     细胞生长动力学模型参数的估算
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  细胞增殖
     Results ET-1 (1×10~ -15 --1×10~ -9 mol/L) enhanced SPC-A1 cell growth in a dose-dependent manner in vitro, with the greatest effect beginning at 1×10~ -11 mol/L.
     结果ET-1(1×10-15~1×10-9mol/L)具有促进SPC-A1细胞增殖作用,作用呈浓度依赖性,在1×10-11mol/L时作用呈饱和状态;
短句来源
     Methods Hela cells were treated with various concentrations (7.5,15,30, 60 mg/L) of As4S4 for different hours(12,24,36,48,60 h). Cell growth was measured by MTT;
     方法以不同浓度(7.5、15、30、60 mg/L)的As4S4对Hela细胞分别处理不同的时间(12、24、36、48、60 h),用四甲基偶氮唑蓝(MTT)法测定细胞增殖反应;
短句来源
     Effect of mPERl on cell growth and migration of NIH3T3 cells
     节律蛋白mPERl在NIH3T3细胞增殖和迁移中的作用
短句来源
     The cell growth curve showed that the proliferation of IFITM1/SW480 was reduced.
     细胞生长曲线显示转染后的IFITM1/SW480细胞增殖活性比对照组增殖活性明显降低.
短句来源
     After transfection with shRNA, SMYD3 expression in HepG2 cells was down-regulated by 75%-85%, and the cell growth was inhibited by 60.95%-72.14%.
     shRNA转染HepG2细胞后:SMYD3蛋白表达下调75%~85%; 细胞增殖明显受抑制,抑制率高达60.95%~72.14%;
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  细胞的生长
     48 hours after medication, the cell growth inhibition rate was 9.33%, 12.70%, 19.70% and 20.93% when the octreotide concentration was 10 -5, 10 -4, 10 -3 and 10 -2 g·L -1 separately.
     结果 :MTT比色法测定显示奥曲肽抑制肝癌细胞的生长 ,在浓度为 10 -5、10 -4、10 -3 、10 -2 g·L-1作用 4 8h生长抑制率分别为 9.33%、 12 .70 %、 19.70 %、2 0 .93%。
短句来源
     (4) hPTTG1 markedly suppressed cell growth and decreased the 〔 3H〕 thymidine incorporation in NIH3T3 cells.
     (4)hPTTG1明显抑制NIH3T3细胞的生长 ,降低〔3 H〕胸腺嘧啶脱氧核苷的掺入率。
短句来源
     When combined treatment of zoledronic acid(1 μmol/L,10 μmol/L)and cisplatin(0.5 mg/L)was used,the cell growth inhibition rates were(44.23 ± 3.77)% and(60.9 ± 3.03)%,respectively.
     唑来膦酸(1、10μmol/L)联合DDP(0.5mg/L)作用A549细胞72h时,对A549细胞的生长抑制率分别为(44.23±3.77)%、(60.9±3.03)%,联合用药组与单药组相比差异有显著性(P<0.01)。
短句来源
     GFP/TRAIL gene was able to inhibit cell growth(93. 5% and 74. 2%)and induce apoptosis(41. 1% and 25. 8%)of DLD-1 and HT-29,respectively, there was significant difference between Ad/hTERT-gTRAIL and the other two control group(PBS and Ad/CMV-GFP)(P<0. 05) ;
     GFP/TRAIL 基因对 DLD1和 HT-29细胞的生长抑制率分别达93. 5%和74. 2%, 凋亡率分别是41. 1%和25. 8%,与 PBS 和 Ad/CMV-GFP 的差异都有显著意义(P<0. 05) 。
短句来源
     The results showed that Eca109 cell growth was inhibited by SVC. while Eca 109 cells were incubated with SVC for 24, 48, 72hrs. The rates of inhibition were 35.6%, 39.5, 36.9% respectively, the concention of SVC used was 0.017μg/ml.
     结果显示,SVC可以抑制Eca109细胞的生长,SVC的浓度为0.017μg/ml,处理细胞24、48、72hr后,细胞生长的抑制率达35.6%、39.5%、36.9%;
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  细胞生长的
     Study on Cell Growth Inhibition of Hela Cells by As_2O_3 and NaAsO_2
     As_2O_3与NaAsO_2抑制HeLa细胞生长的研究
短句来源
     Objective: To transfect the EC1, the esophageal cell line, with NF-κB suppressor gene pcDNA3/mIκBαS32A/S36A and to observe the activity of NF-κB and its effect of cell growth.
     目的:将NF-κB抑制物基因pcDNA3/mIκBαS32A/S36A转染食管癌细胞株EC1,观察对NF-κB因子活性及对细胞生长的影响。
短句来源
     Effect and mechanism of α_(1B)-adrenoceptor on cell growth in DDT1 MF-2
     激动α_(1B)-肾上腺素受体对DDT1 MF-2细胞生长的刺激作用及其途径
短句来源
     Result MTT assay showed that the inhibitory rates on MGC-803 cell growth of different concentrations of DATS 4,8,12,16 and 24 mg·L-1 were 0.231±0.037,0.305±0.036,0.455±0.029,0.607±0.058,0.751±0.019 respectively.
     4、8、12、16、24mg·L-1的DATS对细胞生长的抑制率分别为0.231±0.037,0.305±0.036,0.455±0.029,0.607±0.058,0.751±0.019。 Tunnel检测结果显示,对照组表达阴性,DATS处理组细胞呈阳性表达。
短句来源
     Stable Expression of Caveolin-1 in Murine Hepatocacinoma Cells(Hepa1-6) and Effect of Caveolin-1 on Cell Growth
     Caveolin-1在小鼠肝癌细胞Hepa1-6中的稳定表达及其对细胞生长的影响
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  cell growth
The biological activity of 19peptide was determined by 3-[4,5-dimethylthiazol-2-y1]-2,5-diphenytetrazolium bromide (MTT) assay, cell growth curve, the effect of the ascitic fluid transfevent H22 hepatoma on mice and via histopathological slices.
      
Based on the results, we can draw a conclusion that the two survivin-targeted siRNAs successfully suppressed the expression of survivin mRNA, inhibited cell growth and induce cell apoptosis.
      
Taken together, the inhibition of pirh2 expression in the lung adenocarcinoma cell line A549 resulted in reduced tumor cell growth via the inhibition of cell proliferation, the activation of apoptosis and the interruption of cell cycle transition.
      
Cloning and Expression of the Vitreoscilla Hemoglobin Gene in Enterobacter Aerogenes: Effect on Cell Growth and Oxygen Uptake
      
A novel strain of bacteria (LPM-4) characterized by a unique EDTA requirement for cell growth was isolated.
      
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Observations on the localization of AFP in rat liver tissue in correlation to serum AFP dynamics during 3'-MeDAB carcinogenesis were carried out by the immunoenzyme technique and the radio-rocket-electrophoresis autography.The following results were obtained:(1) During the precancerous stage, AFP was found in a few liver cells in the pseudolobules, in the cells of the basophilio anaplastic regenerative nodules and in some of the "survival" liver cells and "transitional" cells. Most of these cells showed basophilio...

Observations on the localization of AFP in rat liver tissue in correlation to serum AFP dynamics during 3'-MeDAB carcinogenesis were carried out by the immunoenzyme technique and the radio-rocket-electrophoresis autography.The following results were obtained:(1) During the precancerous stage, AFP was found in a few liver cells in the pseudolobules, in the cells of the basophilio anaplastic regenerative nodules and in some of the "survival" liver cells and "transitional" cells. Most of these cells showed basophilio cytoplasm and appeared dedifferentiated.(2) AFP was not detected in the bile duct carcinomas nor in most of the liver cell carcinomas of low grade malignancy, but was demonstrated in the poorly differentiated liver cell carcinomas. The intensity of AFP synthesis in liver cell carcinomas increased in coincidence with their activity in cell growth and cell proliferation but decreased with cell differentiation. AFP synthesis was not directly related to whether or not the cell was in the mitotic stage.(3) In the liver cell carcinomas, the extent of AFP positive reaction as visualized by the immunoenzyme technique basically paralleled the serum AFP level.The possible mechanism and the histologic basis of the serum AFP "saddle shaped" curve during 3'-MeDAB carcinogenesis are discussed, and a preliminary postulation on the histogenesis of liver cancer so induced is suggested.

本工作采用放射火箭电泳自显术和免疫酶标记定位技术,对大鼠3'-MeDAB诱癌过程血清和肝组织甲胎蛋白(AFP)的动态变化和定位情况进行了观察。发现(1)肝硬化假小叶内的少数肝细胞、嗜碱性间变再生结节细胞、少数“幸存肝细胞”及少数“过渡性细胞”,具有合成AFP的能力。未见肝内其他类型细胞合成AFP。合成AFP的细胞大多具有胞浆嗜碱性、生长活跃和形态上去分化的特点。(2)未见胆管癌细胞合成AFP。肝细胞癌分化好的癌细胞绝大多数也未见合成AFP,分化差的癌细胞合成AFP的能力,基本上与其生长活跃程度呈正相关,与分化程度呈负相关,而与癌细胞是否处于核分裂阶段关系不大。(3)肝癌组织AFP酶标的强度和范围与血清AFP水平之间基本上有平行关系。此外,本文还就什么细胞合成AFP、血清AFP“马鞍型”变化的成因及病理组织学基础等进行了讨论,并根据实验结果,对大鼠3'-MeDAB肝癌的组织发生提出了初步的设想。

1.The tongue,urinary bladder,pericardial membrane,lung etc.,altogether 13 kinds of tissues were isolated from the common toad (Bufo bufo gargarizans) and cultivated,the tongue of frog (Bana plancyi) was used for comparison.Observations were made on cell growth and multiplication as well as on other behavior.2.Then the toad kidey and lung tissues as well as frog kidney tissue were selected for monolayer cell cultivation.It was demonstrated that the two commonly used culture media (GLPY and modified...

1.The tongue,urinary bladder,pericardial membrane,lung etc.,altogether 13 kinds of tissues were isolated from the common toad (Bufo bufo gargarizans) and cultivated,the tongue of frog (Bana plancyi) was used for comparison.Observations were made on cell growth and multiplication as well as on other behavior.2.Then the toad kidey and lung tissues as well as frog kidney tissue were selected for monolayer cell cultivation.It was demonstrated that the two commonly used culture media (GLPY and modified Eagle medium) and the method for mono-layer cell culture could be well adapted for amphibian cell culture.3.To isolate cells from esplants for cultivation,the writer found that the use of 0.5% trypsin mixed with 0,01% EDTA and incubated at 25±1℃for a short period of digestion proved to be more satisfactory as compared with the methods used by earlier workers.This new method may be considered as a definite improvement.4.The cytological observations showed that although the epithelium tissues and cells from different sources appeared on the whole quite similar,yet the differences in cell growth and behavior as well as the capacity for multiplication were apparent.In maintaining the original characteristics of the defferent kinds of cells,it appeared that they manifested a certain degree of constancy and irreversibility.Thus it offers a great possibility to use such characteristics for the hybridization study of somatic cells.In this respect,it is to be noted that the ciliary movement of lung tissue from toad,after five months of culture in vitro was still maintained as before without showing least tendency of weakening.The cells of toad's urinary bladder,while migrating outward from the explant to form an outgrowth,showed very little activity of cellular multiplication.It thus appears that cell movement may be considered as a kind of manifestation for cellular differentiation,because it showed a tendency to inhibit cell division.It was further observed that cell division showed a certain degree of synchronization.This may mean that the rate and onset of cell division are determined by cytoplasmic and environmental factors.For example,chick embryonic extract can exert an obvious effect on cell multiplication.The discovery of synchronization as well as non-synchronization of the dinucleated cells and the multipolar divisions of some cells offers problems for further study,especially in view of the fact that a great deal of attention has been paid in recent years to the cytological studies of cell fusion etc.5.In the course of cell division,some abnormalities of metaphase figures,such as chromosome bridge formations were found.This is apparently due to the crossing-over within the heterozygous inverted regions of the chromosomes.While it may be a common occurence in meiosis but in mitosis it is extremely rare.The significance of the observations mentioned was discussed.

作者首先利用组织块培养法研究了中华大蟾蜍(Bufo bufo gargarizans)的舌、膀胱、心包膜、肺脏等十三种组织及金线蛙(Rana plancyi)舌组织的离体培养条件,并观察了在此条件下若干组织在体外生长与增殖的难易程度及表现。然后利用中华大蟾蜍的肾、肺细胞及金线蛙的肾细胞,确立了两栖类脏器组织细胞的单层培养方法。实验证明,本研究所确立的两种培养基(GLPY培养基与修改的Eagle培养基)及单层培养的方法,完全适用于两栖类细胞。在从组织块分离供培养的活细胞时,作者采用的0.5%胰蛋白酶与0.01%EDTA的混合消化液及在常温(25±1℃)下短期消化的方法,效果较好。这和前人在这方面的工作相比较,是一个改进。研究表明,虽然来源不同的上皮组织的细胞在离体培养条件下形态十分相似,但其生长表现与增殖能力却存在相当显著的差异。在各种不同的细胞尚能维持其原有特征这点上,似乎表明,体细胞分化有一定程度的不可逆性。这样,就为研究体细胞的遗传提供了极大的可能性。实验证明,中华大蟾蜍的肺细胞在离体培养五个月之后仍然维持正常的纤毛运动而不见衰退,这说明细胞在离体培养条件下还可以长期维持体细胞的特性。膀胱组织块培养的观察...

作者首先利用组织块培养法研究了中华大蟾蜍(Bufo bufo gargarizans)的舌、膀胱、心包膜、肺脏等十三种组织及金线蛙(Rana plancyi)舌组织的离体培养条件,并观察了在此条件下若干组织在体外生长与增殖的难易程度及表现。然后利用中华大蟾蜍的肾、肺细胞及金线蛙的肾细胞,确立了两栖类脏器组织细胞的单层培养方法。实验证明,本研究所确立的两种培养基(GLPY培养基与修改的Eagle培养基)及单层培养的方法,完全适用于两栖类细胞。在从组织块分离供培养的活细胞时,作者采用的0.5%胰蛋白酶与0.01%EDTA的混合消化液及在常温(25±1℃)下短期消化的方法,效果较好。这和前人在这方面的工作相比较,是一个改进。研究表明,虽然来源不同的上皮组织的细胞在离体培养条件下形态十分相似,但其生长表现与增殖能力却存在相当显著的差异。在各种不同的细胞尚能维持其原有特征这点上,似乎表明,体细胞分化有一定程度的不可逆性。这样,就为研究体细胞的遗传提供了极大的可能性。实验证明,中华大蟾蜍的肺细胞在离体培养五个月之后仍然维持正常的纤毛运动而不见衰退,这说明细胞在离体培养条件下还可以长期维持体细胞的特性。膀胱组织块培养的观察表明,细胞由组织块向外迁移与分裂是有矛盾的,迁移快则分裂少,看来细胞向外迁移也类

1. By means of succesive single-cell culture the effects of ultraviolet irradiation and cytoplasmic amputation on cell division and cell growth in Amoeba discoides were investigated. The effects measured were retardation of division and subsequent accelaration of division, and formation of "giant cell", "small cell" and binucleate cells.

1.应用单细胞分离培养和连续观察的方法,研究了紫外辐射、切割细胞质对变形虫Amoeba discoides细胞生长和分裂过程的各种效应:分裂延迟及随后的分裂加速现象,巨细胞、小细胞和双核细胞的形成; 2.根据本实验获得的种种细胞分裂的异常,并结合文献讨论后认为,细胞的分裂周期与生长周期,是两个既可区分又相联结的生命过程:当细胞分裂周期被阻断时,在一定范围内细胞仍可继续生长;一些细胞在没有或很少生长的情况下仍可通过分裂。细胞分裂周期的启动,需要细胞生长达到临界体积,而细胞可生长的最大体积,受染色体的倍数性所控制。通常情况下,细胞繁殖的限速因子是细胞的生长,而细胞的重复生长有赖于细胞分裂周期的完成。 细胞分裂周期是一个顺次依赖程序,然而各过程间又可相对的区分。当染色体复制或核分裂完毕,而后一过程又被阻断,有时可启动细胞周期的部分重复,以至形成巨细胞和多核细胞。

 
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