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living cell
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  活细胞
     Objective: To study the possibility of enhanced green fluorescent protein (EGFP) gene as informational gene and select mark in living cell, construct the eukaryotic expression vector pCDNA3.1 (+ ) -EGFP for enhanced green fluorescent protein gene, and transfer the eukaryotic expression vector to Hela cell and rat marrow mesenchymal stem cell,then observe the expression of enhanced green fluorescent protein gene in these two types of cells.
     目的:为研究增强型绿色荧光蛋白(enhanced green fluorescent protein,EGFP)基因在活细胞中作为报告基因和筛选标记的可行性,特构建其真核表达载体pCDNA3.1(+)-EGFP,并将它转染至Hela细胞和大鼠骨髓间充质干细胞(mesenchymal stem cell,MSC)中,观察其在这两种细胞中的表达情况。
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     The mechanism of Pb(Ⅱ) sorption by living cell of Trichoderma sp.HR-1.
     木霉(Trichoderma sp.)HR-1活细胞吸附Pb(Ⅱ)的机理
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     The concentration of fetal brain suspension was 4.48×10 7 cells/ml. The living cell was 85.3% and neurocyte 48.2%.
     胎脑细胞悬液浓度为 4.48× 10 7/ml,活细胞占 85 .3 % ,神经细胞占 48.2 %。
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     METHODS The pHi of PLA 801D cells was measured with a fluorescent probe,BCECF,after incubation with dimethyl amiloride (DMA) at various time intervals while the inhibition of reproduction of PLA 801D cells with DMA was observed with the method of living cell count.
     方法 用荧光探针法检测经双甲基氨氯吡咪(DMA) 处理后的PLA801D 细胞内pH(pHi),同时用活细胞计数法观察DMA 对PLA801D 细胞增殖的抑制作用。
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     The drying techniques of active dry yeast of grape wine were studied and the results suggested the following optimum conditions: air intake temperature of drying bed at 80~90 ℃,addition level of emulsifying agent as 1.8 %~2.0 %,water content after drying controlled between 4.5 %~5.5 %,and living cell rate reached about 80 %. (Tran.by YUE Yang)
     对葡萄酒活性干酵母的干燥工艺进行了研究,结果表明,干燥床进风温度以80~90℃为佳,乳化剂加量1.8%~2.0%,干燥后水分控制在4.5%~5.5%,活细胞率达到80%左右。
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  活体细胞
     The Development of an Irradiation Facility for Living Cell in Atmosphere or Solution Environments
     在大气或溶液环境下辅照活体细胞装置的研制
短句来源
     By transformation mediated by Agrobacterium tumefacien, we successfully transferred the chimeric gene of GFP-mTn (mTn is the binding domain of microfilament binding protein talin from mouse, which can show the microfilament in living cell) into Torenia fournieri.
     用农杆菌介导法将嵌合基因CFP-mTn(mTn是微丝结合蛋白Talin的微丝结合域,可以显示活体细胞中微丝的结构)导入蓝猪耳。
短句来源
     The fusion gene ofgfp:mtn (mTn is binding domain of microfilament binding protein talin, which can show the microfilament in living cell) was transferred to Arabidopsis thaliana. Stable expression of GFP-mTn can visualize the actin cytoskeleton in different types in living cells without affecting cell morphology and function.
     将gfp:mtn(mTn是微丝结合蛋白Talin的微丝结合域,可以显示活体细胞中微丝的结构)导入拟南芥后,发现表达的融合蛋白能够标记微丝骨架,同时又不影响植物细胞的正常形态和功能。
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     A three dimensional image of a living cell is helpful for cell secretion study.
     获得活体细胞三维图像以观察细胞内分泌囊泡的空间分布有助于细胞分泌机制的研究。
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     The next part of paper concerned in the method and program to research the spatial distribution and mobility of secretory vesicles of living cell, using the combination of four-dimensional fluorescence microscopy and scientific visualization.
     第三部分重点研究了将四维荧光反卷积显微技术和科学可视化相结合来研究活体细胞中囊泡运动的方法和过程。
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  “living cell”译为未确定词的双语例句
     Results: If the amount of viruses was 100 TCID_(50),10TCID_(50) per 1 000 ml water,the viruses could be detected with living cell absorption,and when the amount was 1~2TCID_(50),the viruses in 13 of 14 samples can also be detected.
     结果:应用活的L20B细胞时,1 000 ml水样中含病毒100TCID50、10TCID50时能全部检出; 当含1~2TCID50时14份样本可检出13份。
短句来源
     The result showed that the coagulation time of L.b-DR and L.b-S1 was 3 h,which was shorter than others and the living cell number of which was more 1×108 cfu/mL,pH was 4.5~5.0 and the titration acidity was 90~100 °T.
     b-S1和L. b-DR凝乳时间最短,为3 h,凝乳后的活菌数、pH值、滴定酸度均无显著差别,活菌数均达1×108 mL-1以上,pH值均达4.5~5.0,滴定酸度均达90~100°T;
短句来源
     when 10 g/L Calcium carbonate was feed to the modified MRS cultural medium(20 g/L glucose,5 g/L peptone,20 g/L beef extract),and 8 g/L glucose was feed at 14 h,the biomass was achieved at 7.12 g/L. The living cell number was 3.89×10~(8) cfu/mL.
     当在改良的MRS培养基中添加质量浓度为10 g/L碳酸钙,并经过一次中间补糖(8 g/L)培养,菌体干重达7.12 g/L活菌数为3.89×108cfu/mL。
短句来源
     The results showed:fermented milk storaged at 7 ℃ for 14 days,the survival rate of two strains of Lactobacillus acidophilus was 3 43% and 2 20%; living cell number reached 108 mL-1; the acidity of milk was 90 °T and 107 °T;
     结果表明,7℃条件下14d两株嗜酸乳杆菌发酵乳中细菌的存活率分别为3.43%和2.20%,且活菌数仍在108mL-1以上,酸度为90°T和107°T,双乙酰分别增加了11.80%和56.41%,乙醛含量分别为2.993mmol/mL和2.561mmol/mL,使发酵乳具有良好风味。
短句来源
     During the cold storage of the yoghourt at 4 ℃,the postacidification of L.b-DR and L.b-S1 was inapparent and a increase in acidity was less than 10 °T,a decrease in pH was 0.2~0.4 and a decrease in living cell number didn't amount to one log rank.
     b-S1和L. b-DR的后酸化活性最低,4℃冷藏21 d,酸度上浮不足10°T,pH值下降0.2~0.4,活菌数下降1个log数量级左右;
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  living cell
The necessity of discharging this energy excess is dictated by the fact that the living cell is a dissipative structure.
      
Thus, realistic haptic rendering techniques have been implemented to validate stable insertion of a micropipette in a living cell.
      
Potassium is the most important ion in the living cell, affecting almost every cellular function.
      
This order, in a living cell, is achieved through specific, but noncovalent, interactions of varieties of structurally dynamic macromolecules under constantly changing physiological conditions.
      
Extraction of membrane proteins from a living cell surface using the atomic force microscope and covalent crosslinkers
      
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Summary The growth requirements of the causal agent of enzootic pneumonia of swine are so fastidious that for seventeen years, since recognizaton of it as an entity by pullar (1948), it had been assumed to be a vitus, until Goodwin and Whittlestone in England, Mare and Switzer in America, near at the same time (1965), could cultivate ie With living cell free media and nominated it as Mycoplasma suipneumonia and M. hyopheumonia respectively. There after, more complex modified media were used to promote...

Summary The growth requirements of the causal agent of enzootic pneumonia of swine are so fastidious that for seventeen years, since recognizaton of it as an entity by pullar (1948), it had been assumed to be a vitus, until Goodwin and Whittlestone in England, Mare and Switzer in America, near at the same time (1965), could cultivate ie With living cell free media and nominated it as Mycoplasma suipneumonia and M. hyopheumonia respectively. There after, more complex modified media were used to promote facility for isolation or subcultivation. In recent years, modified Switzer′s medium Jiangsu medium No.2 (KM2), composed of 50% Eagle′s solution, 30% of 1% lactoalbumin hydrolysate, 20% normal swine serum inactivated at 56℃ for 30 minutes, with additional 1% fresh yeast extract, penicillin 500 unit/ml, thallium acetate 0.0125% and Pheol red 0.002% has been widely used in our country for isolation and subcultivation with more satisfactory results as compared With other media. Yet the costive amino-acids and biotic materials and laborious procedure in prepation of fresh yeast extract expensed more money and much time. Hence, we have tried to use Streptococcus pyogenes or Staphylococcus aureus culture preparation as substitute for Eagle′s solution and yeast extract. The procedures are as follows: 1. Martin broth is inoculated with selected pure culture of Streptococcus pyogenes (or Staphylococcus aureus) and incubated in 37℃ for 24 hours. 2. Centrifuge the culture at 2500 R.P.M. for 30 minutes. 3. Filter the supernatant through sterile seitz filter with F.K. disc under the negative pressure not above 25cm. mercury. 4. Grind the precipitated bacterial ceils with silicon carbide to homogeneous pulp, mix with aliquot of the Cultural supernatant, then filter the mixture through the same seitz filter already used. The filtrate is inocuialed in martin broth and agar slant to test the sterility. The sterile filtrate is dispensed to a number of bottles and kept in frozen state ready for use. The experimental results poited out: 1. The components in bactereal filtrate that promote growth of Mycoplasma suipneumonia are heat labile, though not completely damaged by 56℃ for 30 minutes to Streptococcus and 70℃ 30 minutes to Staphylococcus and do not resist autoclave temperature. 2. The beneficial components to growth of M. suipneumonia exsist both in the cultural fluid and bacterial cells. 3. Survival time: in 37℃, KM_2 culture last at 4th day, the cultures of the media with Strptococeus substitute (Str_50) or Staphylococcus substitute (Sa_50) all last at 8th day; in 4-8℃, KM_2 culture last at 12th day, the cultured cf str_50 last at 26th day and the culture of Sa_50 last at 30th day. 4. Once a certain inoculum of M. suipneumonia added to KM_2 did not show growth any more, but could grow in KM_2 containing 10% Streplococcus preparation. This may be a hint that the preparation of Streptococcus might contain some factors possessing the ability of reviving M. suipneumonia at a critical point. 5. There are no visible differences between KM_2, Str_50 and Sa_50 cultures in ceils′ morphology and pathogenicity, but somewhat diversities in growth rate. 6. Martin broth could be used as substiute instead of 1% lactoalbumin hydrolysate soltrion for subcultivation of M. suipneumonia.

一、以50%的化脓性链球菌或黄金色葡萄状球菌的马丁汤培养物的无菌过滤液(包括过滤的菌体微粒)(代号分别为Str和Sa)、30%的1%水解乳蛋白溶液,20%的正常猪血清(56℃灭活30分钟)(附加物如青霉素500单位/毫升,醋酸铊0.0125%,酚红0.002%)组成的液体培养基,酸度校正至pH7.2~7.4,培养猪肺炎支原体,生长良好。也可用作含1.2%高纯度琼脂固体培养基的基础。含50%链球菌液培养基代号为Str_(50),含50%葡萄状球菌的代号为Sa_(50)。二、在生长旺盛后保存于37℃中,Str_(50)及Sa_(50)培养物均存活8天,而KM_2(由50%的Eagle氏液,30%水解乳蛋白溶液,20%灭活正常猪血清,1%酵母抽出液和附加物如青霉素500单位/毫升,醋酸铊0.125%,酚红0.002%组成)的培养物仅存活4天。在4——8℃冰箱中,Str50培养物存活26天,Sa50培养物存活30天,而KM_2培养物仅存活12天。三、供给生长的成份既存于菌液,也存在于菌体。四、供给生长的成份不耐高热蒸汽灭菌。五、链球菌以65℃30分钟灭活,葡萄状球菌以70℃30分钟灭活,生长成份尚未完全破坏,但不如未...

一、以50%的化脓性链球菌或黄金色葡萄状球菌的马丁汤培养物的无菌过滤液(包括过滤的菌体微粒)(代号分别为Str和Sa)、30%的1%水解乳蛋白溶液,20%的正常猪血清(56℃灭活30分钟)(附加物如青霉素500单位/毫升,醋酸铊0.0125%,酚红0.002%)组成的液体培养基,酸度校正至pH7.2~7.4,培养猪肺炎支原体,生长良好。也可用作含1.2%高纯度琼脂固体培养基的基础。含50%链球菌液培养基代号为Str_(50),含50%葡萄状球菌的代号为Sa_(50)。二、在生长旺盛后保存于37℃中,Str_(50)及Sa_(50)培养物均存活8天,而KM_2(由50%的Eagle氏液,30%水解乳蛋白溶液,20%灭活正常猪血清,1%酵母抽出液和附加物如青霉素500单位/毫升,醋酸铊0.125%,酚红0.002%组成)的培养物仅存活4天。在4——8℃冰箱中,Str50培养物存活26天,Sa50培养物存活30天,而KM_2培养物仅存活12天。三、供给生长的成份既存于菌液,也存在于菌体。四、供给生长的成份不耐高热蒸汽灭菌。五、链球菌以65℃30分钟灭活,葡萄状球菌以70℃30分钟灭活,生长成份尚未完全破坏,但不如未灭活的生长旺盛。六、加10%的Str于KM_2中有促进猪肺炎支原体生长和复苏作用。用于培养棉花拭子深擦病猪喉头和鼻道的滤液,分离率可达77.8%。七、Str_(50)和Sa_(50)制造方法较简单,成本低廉,组成成份容易获得,宜于一般实验室应用和大量生产。八、Sa_(50)中30%的水解乳蛋白溶液可以马丁汤代替,用于接种传代。

A ruby pulsed laser microbeam instrument was applied to the microirradiation and dissection of the living cell. In this paper, the features of integral design of instrument are analysed and are discussed in detail. Theoretical calculation of laser microbeam is described. For obtaining small focusing spot diameter and high focusing efficiency, the better compromise is to choose the microscope eyepiece of low magnification and the microscope objective of high magnification with big numerical aperture. Furthermore,...

A ruby pulsed laser microbeam instrument was applied to the microirradiation and dissection of the living cell. In this paper, the features of integral design of instrument are analysed and are discussed in detail. Theoretical calculation of laser microbeam is described. For obtaining small focusing spot diameter and high focusing efficiency, the better compromise is to choose the microscope eyepiece of low magnification and the microscope objective of high magnification with big numerical aperture. Furthermore, microscope objective must exactly correct spherical aberration and laser is desired the operation with fundamental mode for obtaining small focusing spot size. In this paper, the method of decreasing loss of light energy for laser beam through microscope is discussed in detail, so as to increase efficiency of the focusing system.

红宝石脉冲激光微光束仪用于生物活细胞的微照射和解剖.本文详细分析和讨论了仪器总体设计的特点.论述激光微光束的理论计算.为了获得小的聚焦光斑直径和高的聚焦效率,较佳的折衷方案是选择低倍的显微镜目镜和高倍大数值孔径的显微镜物镜.而且,为了获得小的聚焦光斑尺寸,物镜必须严格校正球差,并且激光器应是基模运转的.文中还详细讨论了减少激光束通过显微镜光能量损失的方法,以提高聚焦系统的效率.

Study of laser microbeam irradiation of biological model tissue and the living cells of plants as well as animals such as soybean cell line, protoplast of mesophyll cells in tobacco, Chinese Hamster Ovary Cell Line (CHO), the 2-cell stage embryos of mouse and rabbit, have been carried out by using XJX-2 ruby laser microbeam system. On the basis of using either naturel chromophore or vital staining, the effective and selective destructions in living cells of plants and animals...

Study of laser microbeam irradiation of biological model tissue and the living cells of plants as well as animals such as soybean cell line, protoplast of mesophyll cells in tobacco, Chinese Hamster Ovary Cell Line (CHO), the 2-cell stage embryos of mouse and rabbit, have been carried out by using XJX-2 ruby laser microbeam system. On the basis of using either naturel chromophore or vital staining, the effective and selective destructions in living cells of plants and animals tested were produced successfully.

利用XJX-2型红宝石激光显微仪对模式组织和大豆胞株,小家鼠及免2-细胞期胚胎等动植物细胞进行了激光显微照射的生物学试验.在利用生物细胞天然发色团或进行活体染色的基础上,对供试的几种动植物细胞产生了有效的选择性损伤.

 
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