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shoot regeneration     
相关语句
  芽再生
     ②the highest shoot regeneration frequency and an average of 3.2, shoots per explant were obtained on MS+BA 0.5mg/L+IBA 0.1mg/L;
     ②愈伤组织在MS+BA0.5mg/L+IBA0.01mg/L培养基上,芽再生频率较高,平均再生芽数为3.2;
短句来源
     The results showed that the callus were preferably induced from the medium of MS+1mg/L IAA+4mg/L 6-BA,and shoot regeneration were better achieved from the medium of MS+0.01mg/L IAA+0.04mg/L 6-BA.
     在MS添加IAA1mg/L和6-BA4mg/L的培养基上诱导的愈伤质量较好,在MS添加IAA0.01mg/L和6-BA0.04mg/L的分化培养基上能够实现较高的不定芽再生
短句来源
     The optimal medium of 4x CMS was MS+0.5 mg·L-1 TDZ+0.5 mg·L-1 NAA+10.0 mg·L-1 AgNO3, and the frequency of adventitious shoot regeneration was 83.3%, the sequence of effecting factors was AgNO3>NAA>TDZ.
     四倍体的不定芽再生优化培养基为MS+0.5mg·L-1TDZ+0.5mg·L-1NAA+10mg·L-1AgNO3(再生率为83.3%),三因素影响的顺序为:AgNO3>NAA>TDZ。
短句来源
     The shoots of Dendrobium regenerated from the calli on the medium MS+BA(1.5mg/L)+NAA(0.2mg/L)+GA(1.0mg/L)+ coco-water(5.0%)+sucrose(3%)+agar(5.5g/L) after about 25 days, and the shoot regeneration rate was 82.0%;
     把愈伤组织接种到MS+BA1.5mg/L+NAA0.2 mg/L+GA1 mg/L+椰子水5%+蔗糖3%+琼脂粉5.5g/L的培养基上诱导不定芽再生,25天左右可看到不定芽的长出,诱导率达82.0%;
短句来源
     shoot regeneration were better achieved f rom the medium of MS + 0.03mg·L-1IAA +1.2mg·L-16-BA.
     L-1IAA和1.2 mg. L-16-BA的培养基上能够实现较高的不定芽再生
短句来源
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  植株再生
     Shoot regeneration from callus re- quired the combinations of IBA 0.5 mg/L with BA 0.1 mg/L, IBA 0.5 mg/L with BA 0.2 mg/L and IBA 1.0 mg/L with BA 0.5 mg/L, and the most suitable combination was IBA 1.0 mg/L and BA 0.5 mg/L.
     从愈伤组织来源的植株再生需要IBA 0.5 mg/L 与 BA 0.1 mg/L、IBA 0.5 mg/L 与BA 0.2 mg/L以及 IBA 1.0 mg/L与 BA 0.5 mg/L的激素组合,其分化的最佳培养基是MS+IBA1.0mg/L+BA0.5 mg/L。
短句来源
     The results were as follows. The effect of hormone was the most significant,the suitable hormone combination was 1mg/L 6-BA+0.5mg/L NAA. The highest shoot regeneration frequency was 92.5%.
     实验结果如下 :激素的影响效应最显著 ,最适激素组合为MS + 6-BA 1mg/L +NAA 0 5mg/L ,植株再生频率平均高达 92 5 1 %。
短句来源
     The results showed that AgNO 3 could significantly increase the percentage of shoot regeneration. The AgNO 3 concentration of 2.5mg/L was the best in this test, which percentage of shoot regeneration and average number of shoot regerneration per explant were 43.3% and 2.77 respectively.
     结果表明 ,AgNO3 2 .5mg/L处理可明显提高子叶外植体的植株再生频率 ,芽再生频率和平均每外植体再生芽数分别达到 4 3 .3 0 %和 2 .77。
短句来源
     An efficient and fast method for plant regeneration from petiole explants with cotyledon was developed in Chinese cabbage( Brassica campestris ssp. chinensis Makino). A MS medium with half strength of NH + 4 concentration and supplemented with BAP 2mg/L,NAA 0.45mg/L and AgNO 3 7.5mg/L was found to be optimum for Chinese cabbage in obtaining the high frequency of shoot regeneration.
     选用 7个白菜类蔬菜栽培品种和 1个杂交亲本进行离体培养植株再生试验 ,筛选出最佳培养基配方为 1/2倍NH+4 浓度的MS培养基附加BAP 2mg/L、NAA 0 .4 5mg/L和AgNO37.5mg/L ,大幅度提高了植株的再生频率 ,高达 84 .8% ,大大缩短成苗周期 ;
短句来源
     Carbenicillin and Cefotaxime had inhibiting effects on callus induction and shoot regeneration at Carbenicillin 500mg/L and Ceftaxime 300mg/L respectively.
     羧苄青霉素 (Carb)和噻孢霉素 (Cef)也抑制韭菜根尖培养的植株再生 ,Cef的抑制作用更加显著 ,Carb 5 0 0mg/L或Cef 30 0mg/L完全抑制不定芽的再生 ;
短句来源
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  芽的再生
     Addition of 2.0 mg L-1 AgNO3 in the medium could greatly increase the frequency of shoot regeneration, and the shoot regeneration rate reached to 86.5%.
     在上述培养基里添加2 mg L~-1的AgNO_3能使芽的再生频率提高至86.5%。
短句来源
     The frequencies and amounts of shoot organogenesis were significantly affected by the hormone concentrations for a given genotype. Compared with Zt, 6-BA had a better effect on inducing shoot regeneration. The regeneration frequency of cultivar CY-1 , CY-2 reached above the level of 95% in MS + 2.0 mg/L 6-BA + 0.02 mg/L IAA.
     不同激素组合及配比对番茄子叶不定芽再生有不同的影响,6-BA对不定芽的再生效果明显优于Zt,CY-1、CY-2子叶在MS+2.0mg/L 6-BA+0.02mg/LIAA的选择性培养基上不定芽再生频率可达95%以上,平均每子叶再生芽数达4个;
短句来源
     2. Shoot regeneration of cucumber cotyledons was promoted by appropriate concentration of AgNO3 (2.0 mg/L) and a higher concentration proved harmful.
     3.适量浓度的AgNO_3可以促进黄瓜离体子叶的再生,通常2.0 mg/LAgNO_3能发挥最好的效果,过量则抑制芽的再生
短句来源
     Ethylene inhibited shoot regeneration,while its antagonist,Ag+,and synthetic inhibitors,AOA and Co+,could significantly improve regeneration frequency.
     乙烯抑制上胚轴不定芽的再生,而乙烯生理作用的拮抗剂Ag+与生物合成抑制剂AOA、Co+可以显著提高不定芽再生频率。
短句来源
     It was found that NAA promoted not only root but also shoot regeneration of Nan Shu 88,8129 4 and Chuan Shu 101.Addition of either 6 BA or ABA to the regeneration medium reduced regeneration rate of internode segment.
     采用南薯88,8129-4和川薯101所作的实验进一步证实,仅添加NAA,甘薯茎段便可直接再生形成植株,而6-BA和ABA的作用依品种而表现出一定的差异.实验还发现色氨酸和谷氨酰胺虽能促进甘薯茎段根再生,但却不利于芽的再生
短句来源
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  高频再生
     High Frequency Adventitious Shoot Regeneration from Hypocotyl Explants in Antirrhinum Majus L.
     金鱼草(Antitthinum majus L.)下胚轴外植体不定芽的高频再生
短句来源
     BA and AgNO 3 can enhance shoot regeneration,and their proper concentration may vary according to different genotypes.
     而对某一种基因型而言 ,激素组成和AgNO3浓度是建立高频再生体系的关键因素。
短句来源
     This article deals with progress in plant tissue culture at home within recent years from the six aspects:fast propagation,embryoid formation,embryo in vitro culture,establishment of efficient shoot regeneration,medical plant culture and chromosome examination
     文章试图从快速繁殖 ,胚状体形成 ,离体胚培养 ,高频再生系统建立 ,药用植物组织培养 ,以及染色体检测等六个方面论述一下近年来国内植物组织培养的进展情况。
短句来源
     Establishment of Efficient Shoot Regeneration System of Chinese Cabbage( Brassica campestris ssp. pekinensis )Inbred Line‘AB 81’and Studies of Transient Expression of gus A Gene
     大白菜AB-81高频再生系统的建立及gusA基因瞬时表达的研究
短句来源
     A shoot regeneration system of high frequency from shoot of Chinese cherry (Prunus pseudocerasus Lindl. ) dwarfing rootstock was established and 19 transformed plants were obtained from the dwarfing rootstock by introducing gus gene and antibacterial polypeptide genes, using Agrobacterium tumefaciens (Smith et Townsend) Conn as mediator.
     建立了樱桃(PrunuspseudocerasusLindl.)矮化砧木茎尖高频再生系统,并利用根癌土壤杆菌(Agrobacteriumtumefaciens(SmithetTownsend)Conn)介导将gus及抗菌肽基因导入樱桃矮化砧木,获得19个转化株系。
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      shoot regeneration
    Functioning in the genotypic background of common wheat cultivar Saratovskaya 29, chromosomes 2R and 3R of rye cultivar Onokhoiskaya stimulated significantly the induction of embryogenic callus highly capable of shoot regeneration.
          
    Rye chromosomes 1R and 6R suppressed the induction of embryogenic callus capable of shoot regeneration.
          
    The frequency of shoot regeneration from somatic cells and tissues of sugar beet varies from 10 to 97% depending on the explant type, culture-medium composition, and genotype.
          
    The effect of the types and concentrations of various cytokines (zeatin, kinetin, and 6-benzylaminopurine) on direct shoot regeneration from cotyledon nodes has been estimated.
          
    The culture-medium composition has been optimized for direct shoot regeneration from petioles.
          
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    The culture of shoot segments taken from grafted trees and nature 2-year-old seedlings of Clausena lansium (Lour) Skeels was successfully established in vitro. Shoot regeneration, root induction and micrograf-ting were achieved.

    1985~1987年,采取无核黄皮、独核黄皮等7个品种(系)的嫩枝茎段进行离体培养,经初代培养、继代增殖、诱导生根或试管嫁接,以及最后进行炼苗移栽等步骤,获得少量试管繁殖的黄皮小植株,在自然条件下生长良好。

    The techniques for rapid micropropagation of velvet ash are described. The stem sections with a bud were used as explants. The results showed: BA, GA, AC, active carbon and carbon source had effects on shoot growth. MS+BA_(3.0-4.0)+IBA_(0.1)+sucrose 3—4.5% was the best medium for shoot regeneration. The rooting of shoots was affected by IBA concentration, PG and light. MS+IBA_(1.5)+PG_(60-120)+sucrose 3% was the best medium for rooting.

    本文对绒毛白蜡微体快速繁殖技术进行了研究,试验采用单芽茎段作为外植体,结果表明:BA、GA、活性炭、碳源对嫩梢增殖生长有影响,MS+BA_(3.0-4.0)+IBA_(0.1)+蔗糖3—4.5%最利于嫩梢的形成。间苯三酚、IBA浓度和光照影响根的诱导,MS+IBA_(1.5)+PG_(60-120)+蔗糖3%最利于生根,黑暗条件下能提高生根率,提早生根时间。本文还提出了绒毛白蜡微体快速繁殖的优化技术程序。

    Protoplasts of Brassica carinata Braun.(accession No.84A165) were enzymatically isolated from hypocotyls and cotyledons of 3-5 day-old test-tube seedlings or first true leaves taken from greenhouse grown plants at a three-leaf stage.The protoplasts were suspended in a P-B liquid medium solidified with 0.15-0.3% low melting agarose which formed a thin layer float- ing on the surface of the liquid medium.The optimum protoplast density was ranging from 5×10~3 to 1×10~4/ml.As for the hypocotyl protoplasts,the first...

    Protoplasts of Brassica carinata Braun.(accession No.84A165) were enzymatically isolated from hypocotyls and cotyledons of 3-5 day-old test-tube seedlings or first true leaves taken from greenhouse grown plants at a three-leaf stage.The protoplasts were suspended in a P-B liquid medium solidified with 0.15-0.3% low melting agarose which formed a thin layer float- ing on the surface of the liquid medium.The optimum protoplast density was ranging from 5×10~3 to 1×10~4/ml.As for the hypocotyl protoplasts,the first division was observed after 48h in the culture.The division frequency reached 21% and 34% at day 3 and 6 respectively.The initiation of celi division in the case of cotyledon and mesophyll protoplast culture was late, usually at day 5,and the division frequency was also somewhat lower.One week after culture, the cultures were transferred to fluorescent light condition with an intensity of about 1,000 1x.A dilution medium DPDK_3 was then added and the dilution procedure was repeated at one week interval thereafter.One month after culture,microcalli with 300-500μm in size were formed,It was also found that in some cases globular embryoid structure protruded on the cal- lus surface.Totally,a 2-3% plating efficiency was achieved.Shoot regeneration occurred when cotyledon and mesophyll protoplast-derived calli were transferred onto a modified MS medium supplemented with NAA 0.1,BA 3mg/l.Individual shoots were rooted on a rooting medium supplemented with 0.2mg/l of IAA.Intact plants with normal morphology were ev- entually produced.

    以埃塞俄比亚芥“84A165”为材料,从3—5日龄无菌苗的子叶、下胚轴或温室生长的三叶期苗的第一真叶游离原生质体,悬浮于 P-B 液体培养基中,用0.15—0.3%低融点琼脂糖固化,薄层漂浮,暗培养。原生质体密度为5×10~3—1×10~4/ml。下胚轴原生质体在培养后48小时出现一次分裂,3天后分裂频率达21%,6天后达34%。子叶和真叶原生质体起始分裂稍晚(第5天),分裂频率也较低。培养1周后,加入稀释培养基 DPDK_3,并转至光下,以后每隔一周加液一次。一个月后获得肉眼可见的小愈伤组织。植板率为2—3%。小愈伤组织在固体增殖培养基上继代长大。子叶和真叶原生质体来源的愈伤组织在转至补加 NAA0.1、BA3mg/1的分化培养基上后获得芽分化,把这些芽切离,转至 IAA0.2mg/l 的生根培养基上,再生出完整植株。

     
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