The sequence accuracy is as high as 99.99%. Using the automatic annotation pipeline developed by NCGR, the 74.5～78.2 cM region of Nipponbare chromosome 4 which includes 19 overlapped BAC clones with a total length of 1946125 bp is analyzed and modified with manual check. 297 candidate genes are discovered in this region including 8 known, 52 novel, 134 putative and 103 hypothetical genes.
The complete sequencing of the human and other genomes allowed studies to be started on positioning of these elements within long multigenic regions of the genome, which is a prerequisite for a comprehensive functional annotation of genomes.
The program has a convenient graphic interface, allowing for navigation in the annotation window, in the genome map, and in the table of gene similarities.
Primary source data preparation and generation of annotation word indices are used to significantly reduce query processing time.
The currently available body of decoded amino acid sequences of various proteins exceeds manifold the experimental capabilities of their functional annotation.
Therefore, in silico annotation using bioinformatics methods becomes increasingly important.