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rna
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  核糖核酸
     Under optimal conditions, the calibration graphs are linear over the range of 2.9-76.0g/mL for fish sperm DNA (FS DNA) , 3.6-68.0g/mL for calf thymus DNA (CT DNA) ,1.1 -36.0g/mL for yeast RNA (YRNA) and 2.9-36.0g/mL for denatured DNA (Da DNA).
     在最佳的实验条件下,鱼精子脱氧核糖核酸(FS DNA)的线性范围为2.9~76.0μg/mL,小牛胸腺脱氧核糖核酸(CT DNA)为3.6~68.01μg/mL,酵母核糖核酸(YRNA)为1.1~36.0μg/mL,变性脱氧核糖核酸(Da DNA)为2.9~36.0μg/mL。
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     Influence of GaAs Ga3+,Ge4+,Hg4+,Hg2+, andCr6+ on DNA Or RNA and that of GaAs on amino acid in activared sludge were studied.
     研究了GaAs、Ga~(3+)、Ge~(4+)、Hg~(2+)和Cr~(6+)对活性污泥脱氧核糖核酸(DNA)和核糖核酸(RNA)的影响,以及GaAs对活性污泥氨基酸的影响。
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     2. epirubicin and RNA , such as RNA Type Ⅲ, RNA Type Ⅵ systems;
     2、RNA Type Ⅲ、RNA Type Ⅵ等核糖核酸与葸环类抗生素表柔比星(EPI)相互作用的反应体系;
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     Construction of hMSH2 antisense RNA expression plasmid1
     hMSH2反义核糖核酸表达质粒的构建
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     Inhibition of Gene KCNJ2 Expression by RNA Interference
     核糖核酸干扰抑制心肌细胞KCNJ2基因表达
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  核酸
     The content of RNA and DNA in the nucleic acid decreases respectively from 2.34μg/mg and 0.96μg/mg to 1.29μg/mg and 0.19μg/mg, which decreases respectively 46.9% and 80.2%;
     核酸中 RNA、DNA的含量分别从 2 . 4 3μg/ mg、 0 .96 μg/ mg分别减少到 1.2 9μg/ m g、 0 .19μg/ mg,分别减少4 6 .9%、 80 .2 % ;
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     Results: AH-SGC 7901, AH-SGC 7901 / VCR, AH-HCC 74O2 and AH-Ec 109 cells transfected with HSP90 antisense RNA all expressed HSP90 antisense RNA.
     结果:HSP90反义核酸转染细胞AH-SGC7901,AH-SGC7901/VCR,AH-HCC7402及AH-Ec109有HSP90反义RNA的表达。
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     The expression of the mouse β_2 microglobulin in NIH3T3 cells transfected with antisense RNA
     小鼠β_2m正反义核酸表达载体转染NIH3T3细胞后的表达
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     The expression of HSP90βprotein in HSP90β antisense RNA vector-transfected cells
     HSP90β反义核酸载体转染细胞HSP90β蛋白的表达
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     Based on the homologous nucleotide sequences of CYP75B5(GenEMBL AF313489)and CYP75B6(GenEMBL AF313488)of Callistephus chinensis,a pair of primers were designed and used to amplify the cytochrome P450 gene from the total RNA of Eupatorium adenophorum by RT-PCR,and a cDNA fragment of 378bp in length was obtained.
     根据菊科植物P450基因C YP75 B5(GenEMBL AF313489)和C YP75 B6(GenEMBL AF313488)核酸序列同源区设计引物,用RT-PCR方法从紫茎泽兰植株中获得一大小为378bp的细胞色素P450基因片段。
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  “rna”译为未确定词的双语例句
     Application of ribosomal RNA gene ITS and IGS regions in the molecular identification and classification fungi
     核糖体RNA基因间隔区ITS及IGS在真菌分子生物学鉴定和分型中的应用
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     Molecular Biology of RNA1-3 of Rice Grassy Stunt Virus
     水稻草状矮化病毒基因组RNA1-3的分子生物学
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     Study on the Gene Expression of Vascular Endothelial Growth Factor (VEGF) & Its Receptors in Human Hepatocellular Carcinoma and the Inhibitory Effect of Antisense VEGF RNA on the Growth of Liver Cancer Cell in Vivo & in Vitro
     人肝细胞癌血管内皮生长因子及受体基因表达和其反义RNA体内外抑瘤作用的研究
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     The Expression of PTP-BAS in Human Primary Renal Cell Carcinoma and the Study on the Difference of RNA Degradation between Renal Cell Carcinoma and Adjacent Normal Tissue
     肾癌BAS基因的表达及肾癌与癌旁组织RNA降解差异研究
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     Effects of Hammerhead Ribozymes and Antisense RNA on NHE-1 Activity and Proliferation in Pulmonary Artery Smooth Muscle Cells of Rats in Vitro
     锤头状核酶、反义RNA对大鼠肺动脉平滑肌NHE-1活性及细胞增殖的作用
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  rna
All compounds studied are significantly active in the RNA-dependent DNA-polymerase (RDDP) assay, and were not toxic toward the Vero cell line.
      
Recently, a class of about 22 nucleotides (nt) small RNA has been discovered in many eukaryotes, termed microRNAs (miRNAs), which have a variety of functions.
      
The RP transgene can be steadily inherited to, and expressed at RNA level, the progenies.
      
RNA interference (RNAi) is a phenomenon of gene silence induced by a double-stranded RNA (dsRNA) homologous to a target gene.
      
In conclusion, the si-RNA-synthesizing plasmids targeting Hsp90β were constructed and transfected into cells with different transfection efficiency.
      
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The sciatic nerve of adult albino rats was first cut and the spinal ganglia of L_5 and L_6 were prepared later for microscopic study. The acid and alkaline phosphatases were prepared by MacDonald's and Danielli's modifications of Gomori's methods respectively. We used methyl green-pyronin for ribonucleic acid (RNA); Baker's acid haematein for phospholipids; Faire and Wolfe's basic fuchsin for mitochondria and both osmic acid and uranium nitrate for Golgi apparatus. Some of the cells began to show eccentric...

The sciatic nerve of adult albino rats was first cut and the spinal ganglia of L_5 and L_6 were prepared later for microscopic study. The acid and alkaline phosphatases were prepared by MacDonald's and Danielli's modifications of Gomori's methods respectively. We used methyl green-pyronin for ribonucleic acid (RNA); Baker's acid haematein for phospholipids; Faire and Wolfe's basic fuchsin for mitochondria and both osmic acid and uranium nitrate for Golgi apparatus. Some of the cells began to show eccentric nucleus and central chromatoly- sis as early as 24 hours after the operation. Others maintained a central nuc- leus and a peripheral chromatolysis even on the third day. Nearly all the cells manifested eccentric nuclei and central chromatolysis on the 6th day. During recovery, the RNA began to increase around the nucleus. It appeared as fine granules at first and became gradually consolidated into the form of Nissl bo- dies. The mitochondria in the injured cells were enlarged and stained deeper. They were increased in number and gathered in the central part of the cell in the degenerating period. The Golgi apparatus was first seen at the periphery of the cell, it then migrated to the center and broke into short rods when the nucleus became eccentric. The activity of acid phosphatase was heightened in the central part of the cell during degeneration and in the early stage of recovery, while the alkaline phosphatase of the cytoplasm was less active. The phospholipid was increased in amount and concentrated at the center of the cell. No neutral fat was found. Plasmal reaction of the cytoplasm show- ed to be stronger than the normal. Besides the changes described above, it was found that the sulphydryl groups were increased in amount in the central part of the cytoplasm. Here the Millon reaction was more intense than the normal. The funetional relationships of the above mentioned organoids and chernical constituents were discussed.

1.本实验系切断大白鼠坐骨神经后,用多种组织学和组织化学方法观察脊神经节细胞内一些细胞器和化学成分的变化。 2.切断坐骨神经后24小时到14天,细胞体积逐渐缩小,核移往细胞周围,染色质溶解。14天后转入恢复时期,体积逐渐增大,核移回细胞中央,尼氏体形成。 3.脊神经节细胞的染色质溶解在早期有二种不同形式,溃变反应较快的细胞,核外移也较早,染色质溶解向细胞中央进行。溃变反应较慢的细胞,核最初仍在细胞中央,染色质溶解在细胞周围发生,不久核也外移,细胞中央尼氏体随着也减少。 4.细胞恢复时核糖核酸首先在核附近增多,细颗粒出现,逐渐增大而具有脊神经节的尼氏体的形状。 5.切断坐骨神经后脊神经节细胞的线粒体增大,着色增深,数量在3天时一度减少,不久又增多,并聚集在细胞中央。细胞恢复时逐渐回复正常。 6.高尔基体在3天时移往细胞周围,随着核的外移又聚集在细胞中央,断裂成短杆状。细胞恢复早期还在核四周,较迟才逐渐分散。 7.随着细胞溃变发展酸性磷酸酶在细胞中央增强,6天时已很显著。细胞恢复时期核四周仍很强,直到恢复后期才回复正常。 8.细胞溃变时核膜和尼氏体环碱性磷酸酶增强。细胞质的酶降低,细胞恢复时逐渐增多,回复正常。 ...

1.本实验系切断大白鼠坐骨神经后,用多种组织学和组织化学方法观察脊神经节细胞内一些细胞器和化学成分的变化。 2.切断坐骨神经后24小时到14天,细胞体积逐渐缩小,核移往细胞周围,染色质溶解。14天后转入恢复时期,体积逐渐增大,核移回细胞中央,尼氏体形成。 3.脊神经节细胞的染色质溶解在早期有二种不同形式,溃变反应较快的细胞,核外移也较早,染色质溶解向细胞中央进行。溃变反应较慢的细胞,核最初仍在细胞中央,染色质溶解在细胞周围发生,不久核也外移,细胞中央尼氏体随着也减少。 4.细胞恢复时核糖核酸首先在核附近增多,细颗粒出现,逐渐增大而具有脊神经节的尼氏体的形状。 5.切断坐骨神经后脊神经节细胞的线粒体增大,着色增深,数量在3天时一度减少,不久又增多,并聚集在细胞中央。细胞恢复时逐渐回复正常。 6.高尔基体在3天时移往细胞周围,随着核的外移又聚集在细胞中央,断裂成短杆状。细胞恢复早期还在核四周,较迟才逐渐分散。 7.随着细胞溃变发展酸性磷酸酶在细胞中央增强,6天时已很显著。细胞恢复时期核四周仍很强,直到恢复后期才回复正常。 8.细胞溃变时核膜和尼氏体环碱性磷酸酶增强。细胞质的酶降低,细胞恢复时逐渐增多,回复正常。 9.细胞溃变时磷脂增多,10天时最显著,聚集在细胞中央。细胞恢复时逐渐回复正常。 10.脊神经节细胞的细胞质反应是阳性,切断周围突起后略见增强。 11.细胞溃变时并没有中性脂物质出现。 12.细胞溃变和恢复早期硫氢基在细胞中央、核膜和尼氏体环等处增强。细胞恢复时逐渐回复正常。 13.Millon反应也在细胞中央和尼氏体环处增深。 14.对于尼氏体、线粒体、磷酸酶、磷脂、硫氢基等细胞器和化学成分的机能关系曾作讨论。

Adult Scfiistosoma japonicum worms obtained from the mesenteric veins of artificially infected mice were studied histochemically for the distribution of nucleic acids, amino acids, glycogen and phosphatases. The results obtained may be summarized below.1. Nucleic acids were seen mostly in the parenchyma cells and reproductive organs. In the testes, DNA was very rich, while RNA was found abundantly in the vitcllaria and ovary.2. The positive reaction for tyrosine, tryptophan and histidine was found in the...

Adult Scfiistosoma japonicum worms obtained from the mesenteric veins of artificially infected mice were studied histochemically for the distribution of nucleic acids, amino acids, glycogen and phosphatases. The results obtained may be summarized below.1. Nucleic acids were seen mostly in the parenchyma cells and reproductive organs. In the testes, DNA was very rich, while RNA was found abundantly in the vitcllaria and ovary.2. The positive reaction for tyrosine, tryptophan and histidine was found in the subcuticular muscles, ovary, testes, vitelline cells and the intestinal epithelium. The bromo-pheriol-blue test for basic proteins was strongly positive in the subcuticular muscles, parenchyma cells and vitellaria, while the positive reaction for ferricyanide method was found only in the cytoplasm of the vitelline cells. 3. Glycogen was richly distributed in. the parenchyma, especially in the region adjacent to the gynaccophoric canal of male worms. It was also present, though in small quantity, in the mature ovum.4. Alkaline phosphatase activity was most pronounced in the Cuticular layer and the epithelial cells of ootypc, while moderately positive reaction was observed in the excretory canal and vitelline cells. Acid phosphatase activity was shown most strongly in the subcuticular muscle layer and the parenchyma cells; the epithelia of the intestinal canal and the sexual glands showed also positive reaction.5. The role played by the substances demonstrated histochcmically in various organs of this fluke was discussed. It is suggested that the phosphatase activity in the cuticular layer might be associated with the active absorption of carbohydrates.

1.应用组织化学的方法研究了从小白鼠肠系膜静脉内取出的日本血吸虫成虫体内核酸、氨基酸、糖元和磷酸酶的分布情况。 2.成虫体内核酸的分布虽较广泛,但以雄虫的睾丸、雌虫的卵巢和卵黄腺中的含量为最高。在睾丸中主要为DNA,而卵巢和卵黄腺中则为RNA。 3.虫体的肌纤维、卵巢和睾丸中的生殖细胞核、卵黄细胞和肠管上皮细胞对显示酪氨酸、色氨酸和组氨酸的偶联重氮反应呈现阳性。角皮下肌层、实质组织细胞和卵黄细胞的颗粒滴呈强的溴酚蓝阳性反应。而对铁氰化物的反应仅卵黄细胞的颗粒滴呈现阳性。 4.糖元主要分布于虫体的实质组织和各种肌纤维内,尤以雄虫抱雌沟附近的实质组织内含量最多。在生殖器官中,仅成熟的卵细胞胞浆含有少许。 5.成虫体表角皮含有大量的碱性磷酸酶,而卵模上皮细胞、卵黄细胞和排泄管壁亦呈阳性反应。酸性磷酸酶主要分布于虫体的角皮下肌层、实质组织细胞核、肠管上皮细胞、雌雄生殖细胞和卵黄细胞内。 6.对于日本血吸虫体内各器官组织中所含的上述各种化学物质的生理意义进行了讨论,并认为血吸虫除肠道摄食外,尚可通过体表角皮吸收碳水化合物等营养物质。

The preceding paper has described the contrasting trophic changes of hypertrophy and atrophy following denervation of the chick latissimus dorsi anterior (ALD) and posterior (PLD) respectively. The present paper studies the corresponding changes in the protein and nucleic acid content of these two muscles. The total protein content as well as its several components including connective tissue, non-connective tissue, sarcoplasmic and contractile protein components were determined. Both RNA and DNA contents...

The preceding paper has described the contrasting trophic changes of hypertrophy and atrophy following denervation of the chick latissimus dorsi anterior (ALD) and posterior (PLD) respectively. The present paper studies the corresponding changes in the protein and nucleic acid content of these two muscles. The total protein content as well as its several components including connective tissue, non-connective tissue, sarcoplasmic and contractile protein components were determined. Both RNA and DNA contents were measured. The periods after denervation chosen for study were 1, 4 and 8 weeks. Comparing normal muscles of the two sides, their protein and nucleic acid contents have been found to be closely similar. Comparing normal ALD and PLD, the former has the higher connective tissue protein, RNA and DNA content and has the lower sarcoplasmic protein content. After denervation, total, sarcoplasmie and contractile protein contents of ALD all increased conspicuously, reaching a maximum at 4 weeks, but their relative values (i.e. per unit muscle weight) showed little change. The connective tissue protein content showed no large increase, so that its relative value sometimes even decreased somewhat. These changes parallelled the development of hypertrophy in ALD following denervation and provided additional confirmation of the genuineness of the observed hypertrophy. For PLD, with the exception of connective tissue protein, all the protein components decreased conspicuously following denervation, parallelling the development of atrophy in this muscle. Their relative value also remained practically unchanged. The connective tissue protein content showed no change, so that its relative value increased with the development of atrophy. For both ALD and PLD, RNA and DNA increased following denervation, though with a different time course. No simple relation could yet be seen between the protein and RNA changes in these two muscles after denervation.

(一)前文描写鸡的前背闊肌(簡称前肌)和后背闊肌(簡称后肌)在去神經后分別表現的肥大和萎縮現象。本文研究此二肌肉去神經后1,4,8周时相应的蛋白貭和核酸含量的变化。 (二)正常鸡的前肌或后肌的左、右两側的蛋白貭,核酸含量是很相近的。但前肌的結締蛋白,RNA和DNA含量高于后肌,而肌浆蛋白浓度則低于后肌。 (三)前肌去神經后随着肥大的发生,从1星期起,总蛋白、肌浆蛋白和收縮蛋白总含量都显著增加,4星期时达高峯,但相对量变化很少;結締蛋白总含量增加不显著,其相对含量有时甚至降低。这些結果証实前肌去神經后的肥大确是真正的肥大。 (四)后肌去神經后随着萎縮的发生,总蛋白、肌浆蛋白和收縮蛋白总含量都显著降低,但相对含量基本不变;結締蛋白总含量不变,因而相对含量显著增加。 (五)前肌和后肌去神經后RNA和DNA含量都增加,但变化时程有所不同。在这两肌肉去神經后的RNA和蛋白貭含量变化之間,还看不出有規律的关系来。

 
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