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      dna
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  基因组dna
    RAPD Analysis on Genomic DNA of Chinese Cabbage and the Other Groups of Brassica
    白菜及其相邻类群基因组DNA的RAPD分析
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    Genomic DNA Extraction and RAPD Analysis of Eggplant and Its Wild Relatives
    几种茄属植物基因组DNA提取与RAPD分析
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    The result showed as follows:by raising the concentrations of antioxidative components and antiphenolic compounds(2% β-ME,2% PVP-40,20 mmol/L Na2S2O5),the purity of the extracted genomic DNA was higher.
    结果表明,适当提高抗氧化、抗酚类物质的浓度(2%β-巯基乙醇,2%PVP-40,20 mmol/LNa2S2O5),提取的基因组DNA纯度较高;
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    Based on the conserved sequence of the chitinase of crucifers,about 1 kb DNA fragment and 5'-end fragment(150 bp)were amplified by PCR from genomic DNA of Chinese cabbage.
    根据已知的几种十字花科植物几丁质酶基因保守序列设计特异性引物,以大白菜叶片基因组DNA为模板,通过PCR反应扩增出约1kb的DNA片段和几丁质酶基因5′端序列片段(150bp)。
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    The effect of different concentrations of template DNA,primer,dNTPs and Mg2+on the PCR reaction was analyzed and the optimized RAPD and ISSR-PCR systems in radish were established.
    对影响PCR反应的主要因子———模板DNA、引物、dNTPs和Mg2+浓度进行优化,建立起适合于萝卜基因组DNA的RAPD和ISSR反应体系。
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  “dna”译为未确定词的双语例句
    Vernalization and Its Relation to DNA Methylation Level in Radish (Raphanus Sativus L.)
    萝卜春化作用及其与DNA甲基化水平的关系
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    Genetic Analysis of the Wild Cherry Germplasm and Identification of Cultivated Cherry Varieties Using DNA Fingerprints
    野生樱桃种质资源的遗传分析及其栽培品种的DNA指纹鉴定
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    Study on the DNA Methylation/Demethylation during Somatic Embryogenesis and Mining of SSR Markers in Citrus
    柑橘胚胎发生过程中DNA甲基化/去甲基化研究及SSR标记开发
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    DNA Content Variation of Calli, Somatic Embryogenesis and Induction of Autotetraploid in Citrus
    柑橘愈伤组织DNA含量变异、体细胞胚胎发生及同源四倍体的诱导研究
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    A Technique for Introducing Exogenous DNA into Cucumber after Self-Pollination
    黄瓜自花授粉后外源DNA导入技术研究
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  dna
       A gene regulatory mechanism has been proposed in which steroid hormones and certain other drugs bind to nuclear receptor proteins followed by transfer to DNA where they are inserted between base pairs.
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       Here, we report using the estrogen receptor that the location of drugs in x-ray crystal structures of the receptors matches closely their predicted spatial locations in the DNA.
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       These findings provide compelling evidence that DNA is the ultimate target of these drugs that act on the human genome.
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       In the present study, two of the probable an umor marine compounds, manzamine A and sarcophine, were screened using benzo[a]pyrene (BP)-derived DNA adduct formation in MCF-7 cells as intermediary biomarker.
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       After 24h incubation, cellular DNA was isolated and analyzed for BP-derived DNA adducts by 32P-postlabeling technique.
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         A higher inductivity of callus formation and about forty thousand embryoids have been achieved by culture of anther from Hevea brasiliensis Muell. Arg. since 1973.From the end of 1977 to the spring of 1978, one hundred and thirteen normal plantlets had grown out of two high-yielding clones--Hal Ken 2 and SCATC 88—13. It is the first time in our academy to establish the plantlets in pots successfully, the survival rate after transplantation was about 30%.Up to now, altogether 137 normal plantlets have been o...
            自1973年培养巴西橡胶花药以来,已产生了诱导频率很高的愈伤组织和近4万个胚状体。在1977年底至1978年春先后从高产无性系“海垦2”和“热研88—13”获得113棵完整植株。并首次盆栽成活,成活率30%。到目前为止,已从“海垦2”、“海垦1”、“热研88—13”等品种中诱导出完整植株137棵,1979年移栽成活率提高到69%。经过几年的试验,得到了培养效果较好的下列培养基:用以诱导愈伤组织和幼小胚状体的第一培养基为MS基本培养基添加5%椰乳、0.5~1.0毫克/升2,4—D和0.5~1.0毫克/升K,蔗糖提高到7~10%,用以继续诱导肉眼可见胚状体的第二培养基为MS基本培养基添加0.1~0.2毫克/升NAA,0.2~1.0毫克/升K和0~10毫克/升水解DNA,蔗糖提高到7%,微量元素可提高1~2倍;用以诱导植株的第三培养基为改良的MS培养基,其大量元素减为60~80%,微量元素加倍,蔗糖提高到5%,再添加2毫克/升GA。对非完整植株,再加0.5毫克/升K和0.5毫克/升BAP,有促进顶芽长出真叶,成为完整植株的作用。接种后45~50天是转移愈伤组织的最适时间。胚状体和植株的诱导频率因材料和花...
文摘来源
         The present experiment showed that, when the apple flower buds were subjected to freezing injury, the green colour of nuclei stained with methyl green was transfered to the red one by pyronin , indicating the degradation of DNA from high polymerization into low polymers ( depo-lymerized DNA ) . Using the red feature of nuclei stained by pyronin as an indicotor, it was exhibited that the primary site of freezing injury was in the vascular bundles of the basal part of flower buds , and then in the...
            苹果花芽遭受冻害时,通过甲基绿—派洛红的染色反应显示细胞核从嗜甲基绿性变为嗜派洛红性,反应DNA从高聚合状态降解到低聚合状态。以核的嗜派洛红性为标志,显示苹果花芽冻害最先发生在花芽基部的维管束,继而是髓细胞,花芽上部的花器官原基分生细胞相对比较抗寒。花芽在寒冬时期(12月中下旬~2月下旬)开始遭到冻害,到早春(3、4月)死亡。抗寒性较弱的‘金冠’花芽在日低温-17℃±开始遭到冻害;抗寒性中等的‘金红’花芽在日低温-20℃±开始受冻;抗寒性强的‘黄太平’在整个越冬过程中绝大部分花芽始终保持正常染色反应,不产生核的嗜派洛红性。
文摘来源
         Using histochemical technique and SEM, the emasculative effect of acridine yellow on the microspores within the anthers of Capsicum frutescens L. var. longum Bail cultured in vitro was examined. The effective concentrations of the reagent used ranged from 100 to 1,000 ppm. It was found that after acridine yellow treatment the syntheses of DNA, ATPase, proteins and polysaccharides were inhibited and the anther cells, microspores and exospores appeared aberrant in different degrees, correlating to the t...
            在组织培养条件下,用组织化学及扫描电镜法比较观察100到1000ppm浓度的吖啶黄对甜椒的杀雄效应,结果表明:药室和小孢子都不同程度的出现畸形。DNA、腺苷三磷酸酶、蛋白质、多糖等合成都受到不同程度的抑制。花粉外壁凹陷变形,并与浓度梯度和培养时间有一定的相关性。
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