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western blotting     
相关语句
  免疫印迹
     Methods Both SW480 cells and wtp53/SW480 cells (SW480 transinfected by wild type p53) were treated with different concentrations of indomethacin, the expressions of CDK2 , CDK4 and p21WAF1/CIP1 proteins were detected by Western blotting.
     方法不同浓度的吲哚美辛作用于细胞株,采用Western斑点免疫印迹方法,分别检测SW480细胞及wtp53/SW480细胞中CDK2、CDK4和p21WAF1/PIC1蛋白的表达水平。
短句来源
     Methods SH-SY5Y cells were treated with 40 nmol/L OA for 0,3,6,12,18,24,36,48 h,and then observed by inverted microscope. Western Blotting was used to detect the levels of phosphorylated tau protein at site Ser~(396) and the change of PTEN in SH-SY5Y cells.
     方法40 nmol/L OA孵育SH-SY5Y细胞不同时间(0,3,6,12,18,24,36,48 h)后,倒置显微镜观察细胞的形态变化,免疫印迹检测各时间点tau蛋白ser396位点的磷酸化及PTEN蛋白水平的变化。
短句来源
     Western blotting indicated that the neuronal PC12 cells expressed VEGF165, Flt-1 and Nrp-1, but Flk-1 had not been detected;
     免疫印迹实验检测到PC12细胞有VEGF165及Flt1和Nrp1的表达,而未检测到Flk1;
短句来源
     Western blotting was used to observe the expression of survivin,Bcl-xL,Bad,Bax,Bcl-2,caspase-9,caspase-3,caspase-6,PARP,DFF45 and lamin B protein.
     采用蛋白免疫印迹(W estern b lotting)法观察凋亡相关蛋白survivin,Bc l-xL,Bad,Bax,Bc l-2,caspase-9,caspase-3,caspase-6,PARP,DFF45和lam in B的表达。
短句来源
     Western blotting was used to test the UⅡ-induced ERK1/2 phosphorylation as well as the effect of Urantide and PD98059 on UⅡ-induced ERK1/2 phosphorylation.
     用免疫印迹技术观察UⅡ诱导后ERK1/2的磷酸化,及Urantide、PD98059对ERK1/2磷酸化的影响。
短句来源
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  蛋白质印迹
     The expression difference of BAG-3 protein 18 hours after cultured with chemotherapy drugs(concentration of drugs: 5-FU 50 μg/ml,MMC 0.5 μg/ml,E-ADM 1.5 μg/ml) of 3 pancreatic cancer cell lines(MIACaPa-2,PANC-1,SW1990) was measured through Western blotting method.
     通过蛋白质印迹方法检测MIACaPa-2、PANC-1及SW1990 3株细胞在化疗药物(5-FU 50μg/ml、MMC 0.5μg/ml及E-ADM 1.5μg/ml)作用18 h后BAG-3蛋白表达的差异。
短句来源
     HSP47 and Vimentin were analyzed in Px and Sx rat with Western blotting to confirm the results of 2DE.
     蛋白质印迹分析蛋白质HSP47、Vimentin在Px大鼠胰腺组织中的表达,以验证2DE的结果.
短句来源
     RAW264.7 cells,a murine ma crophage cell line,cultured in vitro were given with LPS or EGb761+LPS. The NF-κB activity of RAW264.7 cells was tested by Western blotting analysis. The ex pression of TNF-α,IL-1β,IL-6 in RAW264.7 cells were measured by reverse t ranscription polymerase chain reaction techniques(RT-PCR)and enzyme-linked im muno sorbent assay(ELISA).
     分别用LPS或EGb761+LPS处理体外培养的小鼠巨噬细胞系RAW264.7细胞,采用蛋白质印迹分析检测细胞中NF-κB活性,用逆转录-聚合酶链反应(RT-PCR)和酶联免疫吸附法(ELISA)检测细胞中TNF-α、IL-1β、IL-6mRNA和蛋白的表达.
短句来源
     Expression of Kir2.1 channel during human macrophage differentiation into foam cells was investigated by RT-PCR, Western blotting and immunocytochemistry, respectively.
     在建立人巨噬细胞源性泡沫细胞模型的基础上, 采用RT-PCR, 蛋白质印迹及免疫细胞化学方法研究 Kir2.1 通道的表达。
短句来源
     The protein levels of HIF-1α, p-AKT, p-ERK1/2, and P53 were detected by Western Blotting.
     应用蛋白质印迹方法检测芹菜素对MDA-MB-231细胞中HIF-1α,p-AKT,p-ERK1/2,P53表达的时间依赖性和剂量依赖性效应。
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  western印迹
     Establishment of IgE mediated Western blotting in identifying IgE BF/sCD23
     鉴定IgE BF/sCD23的IgE介导的间接Western印迹技术的建立
短句来源
     The expressions of Fas,FasL, Bcl-2,Bax,caspase-3,caspase-8, cytochrome c and phosphorylations of JNK,p38 and extracellular signal-regulated kinase(ERK)1/2 were analysed by Western blotting.
     Fas,FasL,Bcl-2,Bax,caspase-3,caspase-8,细胞色素c的表达以及JNK,p38,细胞外信号调节激酶(ERK)1/2的磷酸化水平用Western印迹检测。
短句来源
     Methods 6A8α-manosidase expression was detected by Western blotting.
     方法Western印迹检测6A8α-甘露糖苷酶的表达。
短句来源
     Methods HIT-T15 cells were incubated with palmitate(0, 0.25, 0.5, 1.0mmol/L) (presence of 11.1mmol/L glucose) for 24 hours before the cells were stimulated with 100nmol/L insulin for 5 min,Western blotting was used to assess the levels of PKB threonine phosphorylation.
     方法 HIT-T15细胞分别与0,0.25,0.5,1.0mmol/L的软脂酸(同时存在11.1mmol/L葡萄糖)孵育24h后,以100nmol/L的胰岛素急性刺激5min,用Western印迹法检测PKB苏氨酸磷酸化水平。
短句来源
     Western blotting showed that differentiation resulted in a 2.08-fold,3.15-fold(P<0.05) and 1.24fold(P>0.05) increment in the expression of SR-BI protein compared with U937 monocytes.
     W estern印迹法中诱导分化组SR-B I蛋白表达量分别是未分化组的2.08(P<0.05)、3.15(P<0.05)和1.24倍(P>0.05)。
短句来源
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  免疫印迹法
     bFGF(300ng/ml,500ng/ml,700ng/ml) ,24hours later the secretion of MMP -1 was detected by western blotting.
     (2)实验组:bFGF(300ng/ml,500ng/ml,700ng/ml),采用Western免疫印迹法分析MMP-1蛋白的分泌情况。
短句来源
     the variation of bcl-2,caspase-9, caspase-8, caspase-3,PRAP protein, acetylated H3 and H4, methylated H3K9 and H3K4 were detected by Western Blotting.
     用蛋白免疫印迹法(Western Blotting)检测PHI对Molt-4细胞的组蛋白H3、H4乙酰化状态和H3K4、H3K9甲基化状态变化。
短句来源
     By using semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and western blotting, we studied the molecular mechanism ( gene of bax、bcl-XL、survivin、pten and protein of Survivin) of the biological effects mediated Ligation of CD40 by srhCD40L on human Burkitt’s lymphoma CA46 cells.
     应用半定量逆转录-聚合酶链反应(RT-PCR)和免疫印迹法检测srhCD40L介导CD40配基化对人类Burkitt淋巴瘤CA46细胞的bax、bcl-xl、survivin、pten基因和Survivin蛋白表达的影响;
短句来源
     By using western blotting, The protein expressions of Survivin、c-Myc、hTERT、NF-ΚB、Bcl-2、Casepase 3 on CA46 cells dealed with Ligation of CD40 by srhCD40L were investigated.
     应用western blot蛋白免疫印迹法检测srhCD40L介导CD40配基化作用于人类Burkitt淋巴瘤CA46细胞后不同时间survivin、c-Myc、hTERT、NF-ΚB、Bcl-2、Casepase 3等蛋白表达的变化。
短句来源
     The results showed that determinated by Western blotting and ELISA,the positive rates of SPIM-Ab in serum and seminal plasma were 30. 0% (32/ 107 ), 29. 0% (36/124)and 33. 6 % (36/107), 31. 5 % (39/ 124 ). respectively, there were no satistical significance (P >0. 05).
     结果表明,免疫印迹法检测血清、精浆SPIM—Ab阳性率分别为30.0%(32/107)和29.0%(36/124),ELISA法检测分别为33.6%(36/107)和31.5%(39/124),两法差异无显著性(P>0.05)。
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  western blotting
Western blotting analysis showed that TM3 protein was purified with affinity purification.
      
The recombinant virus was confirmed using PCR, Southern blotting and Western blotting.
      
The cultural supernatant was collected and tested by SDS-PAGE and Western blotting.
      
Western blotting showed that the protein had a high specificity against mouse-anti-Sj14-3-3 monoclonal antibody and rSj14-3-3 had a promising immune reactivity.
      
Western blotting demonstrated that the pool of 26S proteasomes in ascitic carcinoma Krebs-II was twice that in control lung cells and did not significantly differ by total 26S proteasome quantities from the spleen and liver.
      
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