Methods Both SW480 cells and wtp53/SW480 cells (SW480 transinfected by wild type p53) were treated with different concentrations of indomethacin, the expressions of CDK2 , CDK4 and p21WAF1/CIP1 proteins were detected by Western blotting.
Methods SH-SY5Y cells were treated with 40 nmol/L OA for 0,3,6,12,18,24,36,48 h,and then observed by inverted microscope. Western Blotting was used to detect the levels of phosphorylated tau protein at site Ser~(396) and the change of PTEN in SH-SY5Y cells.
The expression difference of BAG-3 protein 18 hours after cultured with chemotherapy drugs(concentration of drugs: 5-FU 50 μg/ml,MMC 0.5 μg/ml,E-ADM 1.5 μg/ml) of 3 pancreatic cancer cell lines(MIACaPa-2,PANC-1,SW1990) was measured through Western blotting method.
RAW264.7 cells,a murine ma crophage cell line,cultured in vitro were given with LPS or EGb761+LPS. The NF-κB activity of RAW264.7 cells was tested by Western blotting analysis. The ex pression of TNF-α,IL-1β,IL-6 in RAW264.7 cells were measured by reverse t ranscription polymerase chain reaction techniques(RT-PCR)and enzyme-linked im muno sorbent assay(ELISA).
Methods HIT-T15 cells were incubated with palmitate(0, 0.25, 0.5, 1.0mmol/L) (presence of 11.1mmol/L glucose) for 24 hours before the cells were stimulated with 100nmol/L insulin for 5 min,Western blotting was used to assess the levels of PKB threonine phosphorylation.
By using semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and western blotting, we studied the molecular mechanism ( gene of bax、bcl-XL、survivin、pten and protein of Survivin) of the biological effects mediated Ligation of CD40 by srhCD40L on human Burkitt’s lymphoma CA46 cells.
The results showed that determinated by Western blotting and ELISA,the positive rates of SPIM-Ab in serum and seminal plasma were 30. 0% (32/ 107 ), 29. 0% (36/124)and 33. 6 % (36/107), 31. 5 % (39/ 124 ). respectively, there were no satistical significance (P >0. 05).
Western blotting analysis showed that TM3 protein was purified with affinity purification.
The recombinant virus was confirmed using PCR, Southern blotting and Western blotting.
The cultural supernatant was collected and tested by SDS-PAGE and Western blotting.
Western blotting showed that the protein had a high specificity against mouse-anti-Sj14-3-3 monoclonal antibody and rSj14-3-3 had a promising immune reactivity.
Western blotting demonstrated that the pool of 26S proteasomes in ascitic carcinoma Krebs-II was twice that in control lung cells and did not significantly differ by total 26S proteasome quantities from the spleen and liver.