Objective To investigate the effect of interleukin-10 (IL-10) gene transfer on expression of CD44,selectin-E,lymphocyte function associated antigen-1(LFA-1),vascular cell adhesion molecule-1(VCAM-1) in mice heart transplantation rejection.
Compared treatment group with control group at 28 days after gene transfer, the Rentrop scoring was increased (2 8±0 4 vs 1 3±0 8, P <0 001), collateral vessel development assessed by angiography was increased (4 8±0 8 vs 1 7±0 8, P < 0 001), the capillary vessel density was increased (210±19 vs 62±6, P <0 001).
With the goal of developing non-viral techniques for exogenous gene delivery into mammalian cells, we have studied receptor-mediated gene transfer using complexes of plasmid DNA and galactosylated poly-L-lysine, poly(L-Lys)Gal.
To evaluate the optimal parameters for efficient gene transfer into human hepatoma HepG2 cells by the DNA-poly(L-Lys)Gal complexes, the bacterial reporter genes lacZ and cat were used.
Nonviral Gene Transfer Techniques, in Methods in Molecular Biolog
The Dependence of the Efficiency of Gene Transfer with the Use of Lipoplexes Based on New Dicationic Lipids on Their Structure
Lipoplexes based on cholesterol derivatives of oligo(ethylene propylene imines) in gene transfer In vitro and In vivo