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   gene transfer 在 基础医学 分类中 的翻译结果: 查询用时:0.192秒
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gene transfer     
相关语句
  基因转移
    Gene Transfer of huCTLA4-Ig to Induce Immune Tolerance of Rat Liver Graft
    基因转移huCTLA4-Ig诱导大鼠移植肝免疫耐受的实验研究
短句来源
    Study on the Impact of Hot-spot Mutations in HBV/C Gene on the Immune Response of Mice by Gene Transfer Mediated by Recombinant Adenovirus
    用腺病毒载体介导的基因转移研究HBV/C区热点变异对小鼠免疫应答的影响
短句来源
    A new method of ADA isoenzyme electrophoresis and its applification in the experiment of gene transfer
    ADA同工酶醋纤薄膜电泳法的建立及其在基因转移实验中的应用
短句来源
    Construction of Recombinant Retrovirus Vector GTK and Study of HyTK Gene Transfer
    重组逆转录病毒载体GTK的构建及HyTK基因转移的研究
短句来源
    Retrovirus Mediated GM-CSF Gene Transfer into NIH3T3 Cells
    逆转录病毒介导的GM-CSF基因转移入NIH3T3细胞
短句来源
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  基因转染
    The Effect of Adenovirus-mediated Gene Transfer of Bcl-2 on Peripheral Nerve Regeneration and Repair
    腺病毒载体中介Bcl-2基因转染对周围神经再生修复的影响
短句来源
    Chondrogenic Differentiation of Rabbit Mesenchymal Stem Cells Following Gene Transfer of IGF-1
    胰岛素样生长因子-1基因转染对兔骨髓间充质干细胞向软骨细胞分化的影响
短句来源
    DATA SOURCES: Using the of "mesenchymal stem cells,gene transfer", we searched Pubmed database for the articles about gene transfection of MSCs published from January 1999 to January 2007 in English. Meanwhile, we used the above key words in Chinese to search in CNKI database for the same articles published from January 1999 to January 2007. STUDY SELECTION: All the literatures were primarily checked, followed by looking over the quotations in each literature.
    资料来源:应用计算机检索Pubmed1999-01/2007-01间充质干细胞基因转染的文章,检索词"mesenchymal stem cells,gene transfer",文章语言限定为英语,同时检索中文CNKI全文数据库1999-01/2007-01间充质干细胞基因转染的文章,检索词"间充质干细胞,基因转染",文章语言限定为中文。
短句来源
    Testing of Cell Culture Fluid by Recombinant TGFβ_1 Gene Transfer
    重组TGFβ_1基因转染后细胞上清的检测
短句来源
    New Strategies for Enhancement of Retroviral-Mediated Gene Transfer Efficiency into Hematopoietic Stem Cells
    提高逆转录病毒介导造血干细胞基因转染效率的研究策略
短句来源
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  转基因
    Studies on the Regulation of Rat β-Defensin-2 Gene Expression and Gene Transfer in Respiratory Tract
    大鼠β-防御素-2基因在呼吸道的表达调控及转基因研究
短句来源
    Research on the Construction of Retrovirus Vector pBabeNeo-HBD-2 and the Gene Transfer of HBD-2 into Mammary Cells
    移植感染免疫基础研究:逆转录病毒载体pBabeNeo-HBD-2的构建和HBD-2转基因抗感染的研究
短句来源
    Experimental Gene Transfer Study Using Anucleated Reticulocytes as Target Cells
    网织红细胞作为转基因靶细胞的实验性研究
短句来源
    Study on Genetic Immunization of Hepatitis B Virus in Mice by Particle Bombardment-mediated Gene Transfer
    用微弹轰击法介导的转基因对小鼠进行乙肝病毒遗传免疫研究
短句来源
    Advance of Gen Therapy for Neurological Diseases by Direct in vivo Gene Transfer
    直接转基因技术应用于神经系统基因治疗的研究进展
短句来源
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  基因转导
    Apoptosis Induced by Gene Transfer with TGF-β1,Antisense Transcripts to c-myc and ber/abl Genes
    TGF-β1,反义c-myc和反义bcr/abl基因转导诱发细胞凋亡的电镜观察(英文)
短句来源
    Recombinant adeno-associated virus serotype 2-mediated gene transfer to human embryonic cell 293 and human submandibular gland cells
    重组2型腺病毒相关病毒体外基因转导人胚肾293细胞与人颌下腺细胞的研究
短句来源
    Objective : To investigate the effects ofinterleukin 10( I L 10) gene transfer on secretion of inflam matorycytokines in rat cardiomyocytes( C M C) induced by L P Sin vitro .
    目的:观察人白细胞介素10 (human Interleukin 10 ,h I L10) 基因转导对脂多糖( L P S) 诱导大鼠心肌细胞( C M C) 的炎症细胞因子的产生及其基因表达的影响。
短句来源
    ②to observe the effects of interleukin 10 gene transfer on secretion ofinflammatory cytokines in rat C M Ccellsinduced by L P Sin vitro .
    ②观察 I L10 基因转导对 L P S诱导的 C M C 肿瘤坏死因子α( T N Fα) 的m R N A 表达及白细胞介素1β( I L1β) 和 T N Fα的蛋白质的产生的影响。
短句来源
    The effect of Taxol at different concentrations on mdr1 gene transfer cells was determined by FCM.
    用FCM检测不同浓度紫杉醇素对基因转导细胞的作用。
短句来源
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  gene transfer
With the goal of developing non-viral techniques for exogenous gene delivery into mammalian cells, we have studied receptor-mediated gene transfer using complexes of plasmid DNA and galactosylated poly-L-lysine, poly(L-Lys)Gal.
      
To evaluate the optimal parameters for efficient gene transfer into human hepatoma HepG2 cells by the DNA-poly(L-Lys)Gal complexes, the bacterial reporter genes lacZ and cat were used.
      
Nonviral Gene Transfer Techniques, in Methods in Molecular Biolog
      
The Dependence of the Efficiency of Gene Transfer with the Use of Lipoplexes Based on New Dicationic Lipids on Their Structure
      
Lipoplexes based on cholesterol derivatives of oligo(ethylene propylene imines) in gene transfer In vitro and In vivo
      
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We used a replication - defective retrovirus vector containing the human β - globin gene to transfect the ecotropic packaging cell line ψ - 2 ,and then harvested the virus supernatant to infect the amphotropic packaging cell line PA317. As a result , a recombinant vector producing cell line (PIWβ) with a titer of 1. 1 × 105 CPU/ml was isolated . By infecting Friend cells with this high titer virus vector , high efficiency gene transfer was achieved . Southern blot hybridization analysis indicated that...

We used a replication - defective retrovirus vector containing the human β - globin gene to transfect the ecotropic packaging cell line ψ - 2 ,and then harvested the virus supernatant to infect the amphotropic packaging cell line PA317. As a result , a recombinant vector producing cell line (PIWβ) with a titer of 1. 1 × 105 CPU/ml was isolated . By infecting Friend cells with this high titer virus vector , high efficiency gene transfer was achieved . Southern blot hybridization analysis indicated that the intact human β- globin gene was stably integrated into the genome of the target cells. Our results provide a useful reference for further studies of gene therapy of β - thalassemia .

用重组人β-珠蛋白基因的逆转录病毒载体转染单向性的ψ-2包装细胞系,继而感染双向性的PA317包装细胞系,分离到能产生重组逆转录病毒载体的细胞系PIWβ,其分泌的重组载体滴度达1.1×10~5CFU/ml。利用这种缺失性病毒粒子,将人β-珠蛋白基因高效地转移到Friend细胞,Southern印迹杂交结果证实,人β-珠蛋白基因可以完整、稳定地整合到靶细胞的基因组上。以上结果为人β-地中海贫血的基因治疗研究提供了有用的参考途径。

In this study, two expression vectors for recombinant human interleukin-6(rhIL-6):CSV-IL-6(1) and (2) were successfully constructed by routine gene manipulation, PCR amplification, sitespecific mutagensis, and optimionzation of translation. The constructed expression vectors for rhIL-6 were introduced into a simian cell line COS7 using gene transfer by DEAE-Dextran procedure. The expression level of rhIL-6 was determined by EL-6 ELISA using ELISA KIT for quantification of Human Interleukin-6, and the biological...

In this study, two expression vectors for recombinant human interleukin-6(rhIL-6):CSV-IL-6(1) and (2) were successfully constructed by routine gene manipulation, PCR amplification, sitespecific mutagensis, and optimionzation of translation. The constructed expression vectors for rhIL-6 were introduced into a simian cell line COS7 using gene transfer by DEAE-Dextran procedure. The expression level of rhIL-6 was determined by EL-6 ELISA using ELISA KIT for quantification of Human Interleukin-6, and the biological activity of rhIL-6 was assayed by 50% maximum incorporation of ~3H-TdR into 7TD1 cells, an IL-6-dependent hybridoma cell line. At 48h after transfection, the supernatant collected from the culture of COS7 cells transfected with CSV-IL-6 (1) contained rhIL-6 2573pg/ml, and at 72h after transfection it containde 1467pg/ ml; that for CSV-IL-6 (2)were 8261pg/ml and 7101pg/ml, respectively. The biological activity (hybridoma growth factor, HGF) at 48h after transfection from COS-CSV-TL-6 (1) was 10~4U/ ml corresponding to 3.9×10~9U/mg; and that from COS-CSV-IL-6 (2) was 6.3×10~4U/ml mcorresponeding to 7.6×10~9U/mg.

利用基因重组、PCR扩增、定点突变及转译优化等程序,构建了人白细胞介素6重组表达载体CSV-IL-6(1)和CSV-IL-6(2);并利用DEAE-Dextran法转染猴细胞系COS7获得了有生物活性IL-6的暂时性高表达。IL-6ELISA定量测定表达水平:转染后48小时收集的上清CSV-IL-6(1)2573pg/ml,72小时1476pg/ml;CSV-IL-6(2)分别为8261Pg/ml与7101pg/ml。利用IL-6依赖的杂交瘤细胞株7TD1检测转染后48小时收集的培养上清的IL-6生物活性结果为:CSV-IL-6(1)10~4U/ml、3.9×10~9U/mg;CSV-IL-6(2)6.3×10~4U/ml,7.6×10~9U/mg。

Human IL-2 receptor gene was trans fected into Amphotropic viral packaging cell-PA317 cell line by electroporation and calcium phosphate-DNA coprecipitition methods. The results demonstrate eletric field mediated gene transfer is a more efficient technique than the calcium-phosphate method for transfection frequency of retrovi-ral vector in these cells. In the present study several electrical parameters affecting transfection of retroviral vector into viral packaging cell and optinum conditions were evaluated....

Human IL-2 receptor gene was trans fected into Amphotropic viral packaging cell-PA317 cell line by electroporation and calcium phosphate-DNA coprecipitition methods. The results demonstrate eletric field mediated gene transfer is a more efficient technique than the calcium-phosphate method for transfection frequency of retrovi-ral vector in these cells. In the present study several electrical parameters affecting transfection of retroviral vector into viral packaging cell and optinum conditions were evaluated.

本文应用DNA-磷酸钙共沉淀法及电脉冲介导基因转移法将人IL-2受体基因转染Amphotropic病毒包装细胞系-PA317细胞,比较了两种转移方法对逆转录病毒载体的转染效率。实验结果表明,电脉冲介导逆转录病毒载体的转染效率高于磷酸钙沉淀法;并研究了逆转录病毒载体导入病毒包装细胞的部分电场参数对基因转染频率的影响及最适的电转移条件。

 
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