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   gene transfer 在 临床医学 分类中 的翻译结果: 查询用时:0.01秒
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gene transfer
相关语句
  基因转移
    Production of High-titer PA317 Clones and its Efficiency for Gene Transfer
    高滴度产病毒PA317细胞的制备及基因转移效率的研究
短句来源
    The application of adenoviral vector to gene transfer into cardiovascular system
    腺病毒载体在心血管基因转移体系中的应用
短句来源
    Study on comparing gene transfer efficiency in human cord blood and bone marrow CD34~+ cells using FACS
    应用流式细胞仪测定不同来源CD34~+细胞基因转移效率的实验研究
短句来源
    Progress of application of gene transfer system in therapy of angiogenesis
    血管新生性治疗中基因转移系统及其应用的研究进展
短句来源
    Gene transfer technology and genetic radioisotope targeting therapy
    基因转移技术与基因靶向性核素治疗
短句来源
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  基因转染
    Objective To explore the expression of Green Fluorescent Protein Gene transduced into the rats via Sofast reagent (Sofast) and Lipofectamine2000 (Lipo) so as to select the better vector and feasible gene transfer route to submandibular lymph nodes(SMLN).
    目的探讨阳离子聚合物Sofast基因转染试剂(Sofast)和阳离子脂质体Lipofectamine2000(Lipo)经不同途径向大鼠转入绿色荧光蛋白基因(PEGFP-N2),观察其在颌下淋巴结(SMLN)的表达。
短句来源
    Study of myocardial perfusion of porcine chronic ischemic myocardium before and after gene transfer
    猪慢性缺血心肌基因转染前后心肌灌注图像的研究
短句来源
    Conclusion:This gene transfer system may not induce growth change and anti double strand DNA antibody in the transfected cells.
    结论 :分子外科缝线术转染外源基因的方法不会引起细胞生长行为的改变和诱发抗双链 DNA抗体的产生 ,是一种安全的基因转染方法
短句来源
    Conclusion TGF βRⅡ/IgG Fc gene transfer effectively abrogates CsA induced tubulointerstitial injury and fibrosis which further proves that TGF β plays an important role in CsA nephrotoxicity.
    结论 TGF βRⅡ /IgGFc基因转染有效抑制了CsA引起的小管间质病变。
短句来源
    Methods:Gene transfer group (Ⅰ) includeed 6 pigs and control group (Ⅱ) 5 pigs.
    方法 :基因转染组猪 6头 ,对照组猪 5头。
短句来源
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  “gene transfer”译为未确定词的双语例句
    Study on the Expression and Time Course of the Cene Transfered by Polylysine Coated Balloon Catheter Local Gene Transfer System
    多聚赖氨酸包被球囊导管转移基因表达的时相特征
短句来源
    Comparative study on in vivo direct intratumal gene transfer cationic liposome-tk and nude HSV-tk gene in mice
    应用阳离子脂质体及裸HSV-TK基因瘤体内直接注射比较研究
短句来源
    Analysis of the Risk of Retroviral-mediated Gene Transfer into Hematopoietic Stem Cells
    逆转录病毒介导的造血干细胞基因治疗的风险性分析
短句来源
    Successful gene transfer and integration of NeoR into NIH3T3 cells was confirmed by PCR.
    以NIH3T3细胞为靶细胞证实NeoR基因可被重组病毒转移至靶细胞基因组,巢式PCR与标志基因补救分析均未能检测到野生型辅助病毒。
短句来源
    On day 10 after gene transfer, the expression level of HGF was evidently higher in local muscles transferred with pUDKH than those with the blank plasmid (pUDK) via both gene gun and intramuscular injection.
    转移pUDKH后第 10天 ,用基因枪和直接注射法转移的局部肌肉组织内HGF的表达明显高于转移空白质粒 (pUDK)的对照组 ;
短句来源
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  gene transfer
With the goal of developing non-viral techniques for exogenous gene delivery into mammalian cells, we have studied receptor-mediated gene transfer using complexes of plasmid DNA and galactosylated poly-L-lysine, poly(L-Lys)Gal.
      
To evaluate the optimal parameters for efficient gene transfer into human hepatoma HepG2 cells by the DNA-poly(L-Lys)Gal complexes, the bacterial reporter genes lacZ and cat were used.
      
Nonviral Gene Transfer Techniques, in Methods in Molecular Biolog
      
The Dependence of the Efficiency of Gene Transfer with the Use of Lipoplexes Based on New Dicationic Lipids on Their Structure
      
Lipoplexes based on cholesterol derivatives of oligo(ethylene propylene imines) in gene transfer In vitro and In vivo
      
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In the present study, we prepared cationic liposomes encapsulated IL-2 DNA or IL-6 DNA with reverse-phase evaporation to investigate their efficiency in mediating gene transfer. After transfection with IL-2 DNA liposome or IL-6 DNA liposome, the supernatants of NIH3T3 cell contained high levels of IL-2 or IL-6. The peritoneal cells from the mice injected i.p. of IL-2 DNA or IL-6 DNA liposome 10 days later could also be detected to secrete IL-2 or IL-6. The B16F10 melanoma cells from tumor-bearing mice...

In the present study, we prepared cationic liposomes encapsulated IL-2 DNA or IL-6 DNA with reverse-phase evaporation to investigate their efficiency in mediating gene transfer. After transfection with IL-2 DNA liposome or IL-6 DNA liposome, the supernatants of NIH3T3 cell contained high levels of IL-2 or IL-6. The peritoneal cells from the mice injected i.p. of IL-2 DNA or IL-6 DNA liposome 10 days later could also be detected to secrete IL-2 or IL-6. The B16F10 melanoma cells from tumor-bearing mice which were injected intratumorally of IL-2 DNA or IL-6 DNA liposome exhibited resistant to G418 and secreted high levels of IL-2 or IL-6. The results show that the liposome can mediate cytokine gene transfer efficiently in vitro and in vivo.

本研究用反相蒸发技术制备出包裹IL-2 DNA或IL-6 DNA的脂质体,将其与体外培养的靶细胞共育,或将其直接注射至小鼠腹腔内及荷瘤小鼠的瘤体内,研究其介导体内外基因转移的效果,结果表明我们研制的脂质体不但能将目的基因转移至体外培养的靶细胞中,并可通过腹腔内注射及瘤体内注射后,在腹腔内及肿瘤原位直接将IL-2和IL-6基因转移至腹腔细胞及肿瘤细胞中,稳定表达IL-2和IL-6。

In order to use retroviral-mediated gene transfer technology in clinical application, retroviral vector must be of high titer and free of detectable replication-competent retroviruses (RCR). The aim of this study was to optimize methods of defective retroviral vector production. Study was conducted using a LXSN vector inserted with human tumor necrosis factor-α gene and an amphotropic retrovirus packaging cell line-PA317. The results indicated that viral titer was influenced by volume of medium and...

In order to use retroviral-mediated gene transfer technology in clinical application, retroviral vector must be of high titer and free of detectable replication-competent retroviruses (RCR). The aim of this study was to optimize methods of defective retroviral vector production. Study was conducted using a LXSN vector inserted with human tumor necrosis factor-α gene and an amphotropic retrovirus packaging cell line-PA317. The results indicated that viral titer was influenced by volume of medium and concentration of fetal calf serum. Inactivation of retroviral vector was greater at 37癈 than at 32癈. In experiment of transfection of PA317 and transduction of 3T3, integration of retroviral vector into genome of packaging cells and target cells, and free of RCR were detected by polymerase chain reaction analysis. Viral vector with high titer and free of RCR is able to use in clinical trial

逆转录病毒介导的基因转移技术要过渡到临床应用,主要解决如何使病毒载体具有高滴度而不具有复制活性。本文旨在探索建立一种合适的方法,能产生高滴度而且是安全的逆转录病毒载体。采用带有人肿瘤坏死因子基因的LXSN载体转染PA317包装细胞,建立产病毒的包装细胞株,结果表明:病毒载体滴度受包装培养液体积和胎牛血清浓度影响;包装细胞在32℃产生的病毒滴度比37℃高,而且比较稳定。产病毒包装细胞和被感染的靶细胞经PCR分析显示,被转染和被转导的细胞都有病毒基因的整合,而且包装细胞不会产生具有复制活性的逆转录病毒,因此能进一步用于临床研究。

irus titer of retrovirus vector is one of the most crucial factors that effect gene transfer effi-ciency.By repeated supernatant infection of ecotropic producer clone on amphotropic producerclone and ping-pong supernatant infection between ecotropic prducter clone and amphotropicproducer clone,the virus titer of amphotropic PA317 preducer clone could be raised by one or twoorders, and no replication-competent virus was detected. Human mononuclear bone marrow cellswere infected repeatly with high-titer...

irus titer of retrovirus vector is one of the most crucial factors that effect gene transfer effi-ciency.By repeated supernatant infection of ecotropic producer clone on amphotropic producerclone and ping-pong supernatant infection between ecotropic prducter clone and amphotropicproducer clone,the virus titer of amphotropic PA317 preducer clone could be raised by one or twoorders, and no replication-competent virus was detected. Human mononuclear bone marrow cellswere infected repeatly with high-titer amphotropic virus vectors and non-high-titer virus vectorsunder stimulation of equal hematopoietic growth factors,Southern blot hybridization analysis indi- cated that only high-titer virus vectors could transfer more gene into human hematopoietic cells.

逆转录病毒载体的病毒滴度是影响其基因转移效率的最关键因素之一。用产病毒载体ψ-2细胞克隆的上清重复感染产病毒载体的PA317细胞克隆和用这两种产病毒细胞克隆进行乒乓上清感染,均能使PA317细胞克隆的病毒滴度提高1~2个数量级且无辅助病毒产生。在同样的造血生长因子应用下,以不同滴度的病毒载体重复感染人骨髓单核细胞,Southern印迹杂交显示,只有高滴度的病毒载体才能将目的基因较多地转移到人骨髓单核细胞中。

 
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