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gene transfer
相关语句
  基因转移
    Effects of Adenovirus HuCT-1 Gene Transfer on Spinal Cord Injury in Rats
    腺病毒介导人心肌营养素-1基因转移对大鼠脊髓损伤的修复作用
短句来源
    In Vivo Gene Therapy for Sciatic Nerve Injury of Adult Rats by Adenovirus-mediated Gene Transfer of BDNF
    腺病毒介导的BDNF基因转移治疗大鼠坐骨神经损伤
短句来源
    Effects and Mechanisms of Adenovirus-mediated BDNF and huCT-1 Gene Transfer and Training on Plasticity after Incomplete SCI in Rats
    腺病毒介导的BDNF、CT1基因转移和功能训练对脊髓损伤后可塑性变化的作用及可能机制
短句来源
    Study of Combined Gene Transfer of IGF-I and HSV-tk for Optimization of Wound Healing
    IGF-I和HSV-tk联合基因转移优化创面愈合的研究
短句来源
    Effects of Adenovirus N-Bak Gene Transfer on Rubrospinal Tract Injury in Rats
    腺病毒介导N-Bak基因转移对大鼠红核脊髓束的修复作用
短句来源
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  基因转染
    Experimental Study on Cardiac Xenotransplantation Tolerance Produced by Inducing of the Mixed Chimerism Using BMC/BM-MSCs Combined with TGF-β_1 Gene Transfer
    TGF-β_1基因转染联合BMC/MSCs诱导嵌合体在异种心脏移植免疫耐受中作用的实验研究
短句来源
    An Experimental Study on Stable Expression of hBMP-2 Gene and Induced Osteogenesis by Gene Transfer of hBMP-2 into Fibroblasts
    人骨形态发生蛋白2基因转染成纤维细胞后的稳定表达和诱导成骨的实验研究
短句来源
    Expression of MMP3 mRNA in hypertrophic scar fibroblasts and MMP3 gene transfer
    瘢痕成纤维细胞的MMP-3 mRNA表达及其基因转染
短句来源
    The effect of FasL gene transfer to islet cells on pancreatic islet allografts
    胰岛FasL基因转染对大鼠胰岛移植的影响
短句来源
    Study on the Potential Clinical Significance of Intimal Hyperplasia Inhibition after Autologous Vein - to - Artery Transplantation by Overexpression of Adenoviral Mediated p21 Gene Transfer
    p21基因转染治疗移植静脉内膜增生的潜在临床价值研究
短句来源
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  转基因
    An Experimental Study on Preventing Denervated Skeletal Muscle Atrophy by Myogenin Gene Transfer
    肌细胞生成素(myogenin)转基因防治失神经骨骼肌萎缩的实验研究
短句来源
    Experimental Study of Cationic Lipsome-medicated Insulin-like Growth Factor-1 Gene Transfer in Vivo on Acute Spinal Cord Injury in Rats
    阳离子脂质体介导胰岛素样生长因子-1体内转基因治疗脊髓压迫性损伤的实验研究
短句来源
    Construction of recombinant adenoviral vector Ad - CMV - hTGF β1 for reversion of intervertebral disc degeneration by gene transfer.
    转基因逆转椎间盘退变重组腺病毒载体Ad/CMV-hTGFβ1的构建
短句来源
    Experimental study on ex vivo gene transfer intracoronarily to donor heart
    心脏移植中经冠脉系统转基因的实验研究
短句来源
    Effects of pre-disposal treatment with pEGFP-N1-IGF-1 gene transfer on nitric oxide synthase activity after spinal cord injury in rats
    重组pEGFP-N1-IGF-1体内转基因预处理对脊髓损伤后一氧化氮合酶活性的影响
短句来源
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  “gene transfer”译为未确定词的双语例句
    Effects of Adenovirus-mediated BDNF Gene Transfer into Spinal Cord to Treat Nerve Root Injury and Its Mechanism
    腺病毒介导的BDNF基因脊髓内转移治疗神经根损伤及其机理
短句来源
    Adenovirus-mediated Gene Transfer of MC148 to Block β Chemokines
    腺病毒介导MC148基因对β趋化因子的拮抗作用研究
短句来源
    The Experimental Studies of vIL-10 Gene Transfer of Hematopoietic Stem Cells Induce Tolerance to Murine Cardiac Allograft
    vIL-10基因修饰造血干细胞诱导小鼠心脏移植免疫耐受的实验研究
短句来源
    Decompressive Craniectomy, VEGF Gene Transfer and Combined Therapy for Malignant Middle Cerebral Artery Infarction in Rats
    大骨瓣减压术、VEGF基因治疗及联合治疗恶性大脑中动脉梗死
短句来源
    Experiment of Liposome Mediated Glial Growth Factor Gene Transfer in Vivo on Recovery of Spinal Cord Injury in Rats
    脂质体介导胶质细胞生长因子基因体内转染对大鼠脊髓损伤修复作用的实验研究
短句来源
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  gene transfer
With the goal of developing non-viral techniques for exogenous gene delivery into mammalian cells, we have studied receptor-mediated gene transfer using complexes of plasmid DNA and galactosylated poly-L-lysine, poly(L-Lys)Gal.
      
To evaluate the optimal parameters for efficient gene transfer into human hepatoma HepG2 cells by the DNA-poly(L-Lys)Gal complexes, the bacterial reporter genes lacZ and cat were used.
      
Nonviral Gene Transfer Techniques, in Methods in Molecular Biolog
      
The Dependence of the Efficiency of Gene Transfer with the Use of Lipoplexes Based on New Dicationic Lipids on Their Structure
      
Lipoplexes based on cholesterol derivatives of oligo(ethylene propylene imines) in gene transfer In vitro and In vivo
      
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t-PA (Tissue Plasminogen activator) gene therapy may provide a novel therapeutic strategy in preventing the thrombotic diseases.The goal of this study was to detect the expression duration ofthe t-PA gene transferring into the arterial wall in vivo and whether the gene therapy was able to preventrestenosis after PTCA. The retroviral vector containing t-PA cDNA was directly delivered into 10 coronar-ies and 12 femoral arterial walls of 12 dogs. 6 coronaries of 6 dogs in control group were perfused...

t-PA (Tissue Plasminogen activator) gene therapy may provide a novel therapeutic strategy in preventing the thrombotic diseases.The goal of this study was to detect the expression duration ofthe t-PA gene transferring into the arterial wall in vivo and whether the gene therapy was able to preventrestenosis after PTCA. The retroviral vector containing t-PA cDNA was directly delivered into 10 coronar-ies and 12 femoral arterial walls of 12 dogs. 6 coronaries of 6 dogs in control group were perfused TEbuffer via porous perfusion balloon catheter after canine restenotic model were established. The existenceof t-PA cDNA and the expression of active t-PA products were proved by hybridization in situ ,mRNA dotblot and immunohistochemistry on 30,60,and 90 days. Our results showed that the percent area of stenosis decreased more than 20% in gene therapy group compared with control one ,and further demonstratedthat the t-PA gene therapy was able to decrease neointimal proliferation in the restenotic model.

用18条犬探讨了组织型纤溶酶原激活剂(t-pA)基因治疗预防经皮冠状动脉成形术(PTCA)后再狭窄的应用价值,其中对照组6条犬.12条在建立再狭窄模型时即用多孔球囊输注导管将t-pA基因逆转录病毒载体直接注入血管壁,经过30、60、90d不同时间的观察,结果表明,在DNA水平(原位杂交,Southernblot)、转录水平(mRNA打点杂交)及产物水平(免疫组化)等方面均证实了t-PA基因的存在和活性产物表达,而且发现这种活性t-pA使再狭窄犬模型的百分狭窄面积在3个月时减少了22.2%,有较好的预防再狭窄作用。

Objective . This study was undertaken to test the feasibility of local gene transfer in vivo us-ing polylysine- coated balloon catheter. Methods . Ad5 vector with reporter gene and empty Ad5 vector were locally transfered into experimental or control arteries. respectively.Results. with the local gene delivery system. reporter gene was successfuly transfeied into the artery wall.Concluslon. The polyly-sine- coated balloon catheter is feasible in in vivo local gene transfer.

目的:研究多聚赖氨酸包被的球囊导管作在体定向基因转导的可行性。方法:将带有报告基因的腺病毒载体和空载腺病毒分别导入实验及对照组动物的特定动脉。结果:应用该系统,成功地将报告基因导入兔动脉壁中。结论:该局部基因转导系统可用于在体定向基因转导。

It is aimed to coat eithr stent for implantation following PTCA or Dacron proarhesis (<4mm in diameter) for coronary artery bypass graft by endothelial cells (ECs) with high efficiency expression of human Pro-UK cDNA so as to prevent thrombosis.This report is the first part of the whole study.The retroviral vector PN2-CMV-UK containing the human Pro-UK cDNA were transfected into the packaging cells PA317 by mean of coprecipitation with calcium phosphate. The supernatant containing the recombinant retrovirus...

It is aimed to coat eithr stent for implantation following PTCA or Dacron proarhesis (<4mm in diameter) for coronary artery bypass graft by endothelial cells (ECs) with high efficiency expression of human Pro-UK cDNA so as to prevent thrombosis.This report is the first part of the whole study.The retroviral vector PN2-CMV-UK containing the human Pro-UK cDNA were transfected into the packaging cells PA317 by mean of coprecipitation with calcium phosphate. The supernatant containing the recombinant retrovirus with the Pro-UK RNA. sequence was collected,Fetal bovine aortic ECs were isolated by mechanical scraping and cultured routinely. ECs within the 7th passage were infected with the supernatant and screened with G418. Southern blot analysis demonstrated the presence of pro-UK cDNA in the genome of the transduced ECs. Immunohistochemical assay showed that a positive brown granules of human Pro-UK antigen was present in the cytoplasm of transduced ECs while a negative result in control ECs. The rate of Pro-UK secretion from traneduced ECs was about 23U/106 cells/24hr but no fibrinolytic activity occurred in control ECs. The result indicated that bovine aortic ECs integrated with human ProUK cDNA were capable of expressing immunoreactive and functional Pro-UK which suggest that Pro-UK gene transfer can enhance fibrinolytic activity of ECs.

本实验将人的尿激酶原(Pro-UK)cDNA导人体外培养的胎牛主动脉内皮细胞(EC),得到了表达该基因的转化细胞,以期覆盖血管内支架或小口径人工血管(<4mm)等移植物达到防止血栓形成,提高移植物通畅率的目的。利用磷酸钙盐沉淀法将重组逆转录病毒载体pN2-CMV·UK导人包装细胞PA317,获得了含有重组逆转录病毒(Pro-UKRNA序列)的培养上滑。用机械刮取胎牛主动脉内腔面分离EC,进行常规培养。用重组逆转录病毒感染7代以内的牛EC,并经G4l8筛选得到抗性细胞。Southernblot分析表明ProUKcDNA已整合进EC基因组。以鼠抗人的Pro-UK单克隆抗体为一抗,作细胞免疫组化分析(L5A8法),抗性细胞胞浆中出现阳性棕色颗粒,对照细胞为阴性结果。溶圈实验测得尿激酶原分泌量约为23U/106细胞/24小时。上述结果证明人Pro-UKcDNA已整合入牛EC基因组,表达产物具有免疫活性和纤溶酶原激活作用。

 
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