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   gene transfer 在 眼科与耳鼻咽喉科 分类中 的翻译结果: 查询用时:0.011秒
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gene transfer
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  基因转移
    Endostatin Gene Transfer for Inhibition of Retinal Angiogenesis in Mouse
    内皮抑素基因转移抑制视网膜新生血管的实验研究
短句来源
    The development in the studies of virus vector-mediated gene transfer in retina
    病毒载体介导的视网膜基因转移研究进展
短句来源
    Preparation of green fluorescent protein retrovirus and its application in mediating gene transfer into retinal pigment epithelial cells
    绿色荧光蛋白逆转录病毒及其介导的视网膜色素上皮细胞基因转移研究
短句来源
    Therefore, strategy of anti-angiogenesis aimed at soluble VEGFR by highly effective gene transfer techniques is becoming a big focal point for gene therapy.
    以高效的基因转移技术针对可溶性VEGFR以实现抗肿瘤新生血管生成的策略就成为基因治疗的一大切入点。
短句来源
    Electroporation is the most effective non-viral technique for exogenous gene transfer, and it has been applied extensively due to its convenience andhigh efficiency.
    电穿孔基因转移技术是外源基因转移最为有效的一种非病毒载体法,其突出的优点是简便、高效,已广泛用于体外基因转移
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  基因转染
    Experimental Study of the Fas Ligand Gene Transfer to Corneal Tissues of Rats Mediated by Adenovirus
    腺病毒介导FasL基因转染大鼠角膜组织的实验研究
短句来源
    Adenovirus-mediated NT3 gene transfer protects spiral ganglion neurons from degeneration after noise trauma
    腺病毒介导的NT3基因转染对噪音损伤的耳蜗螺旋神经节细胞的保护作用
短句来源
    Gene transfer of adenovirus to murine ocular tissues
    腺病毒介导基因转染活体眼组织的研究
短句来源
    Experimental Investigation of pEGFP-bFGF Gene Transfer to Human Limbal Stem Cells
    pEGFP-bFGF基因转染人角膜缘干细胞的实验研究
短句来源
    Conclusions:The transfection efficiency of rAd is higher than that of LF2000 in cultured retinal photoreceptors,and rAd is an optimal gene transfer method for gene therapy in retinal degenerative diseases.
    结论 :在视网膜感光细胞的基因转染中 ,腺病毒介导的目的基因bcl-XL 的转染率及表达明显高过脂质体法 ,重组腺病毒介导的基因转染法是视网膜变性性疾病基因治疗的一种较理想方法。
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  “gene transfer”译为未确定词的双语例句
    Adenovirus mediated LacZ gene transfer into retinal pigment epithelial cells
    腺病毒介导的LacZ基因对视网膜色素上皮细胞的转导
短句来源
    Experimental Study of Plasmid TGF-β_1 DNA Gene Transfer with Lipofectamine to Rabbit Corneal Epithelial Cells in vitro
    脂质体介导TGF-β_1质粒DNA转染家兔角膜上皮细胞的实验研究
短句来源
    Experimental observation of plasmid TGF-β_1 DNA gene transfer with lipofectamine to rabbit corneal epithelial cells in vitro
    脂质体介导TGF-β_1质粒DNA转染家兔角膜上皮细胞的实验研究
短句来源
    Adenovirus-mediated herpes simplex virus thymidine kinase gene transfer under the driving of KDR promoter in treatment of experimental human nasopharyngeal carcinoma in nude mice
    腺病毒介导KDR启动子的胸苷激酶基因治疗小鼠人鼻咽癌模型
短句来源
    Experimental Study of Protection and Treatment of bFGF Gene Transfer Through an Intact Round Window Membrane into Cochlea on Explosive Deafness in Rat
    经完整圆窗膜途径bFGF基因大鼠耳蜗内转导防治爆震性聋的实验研究
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  gene transfer
With the goal of developing non-viral techniques for exogenous gene delivery into mammalian cells, we have studied receptor-mediated gene transfer using complexes of plasmid DNA and galactosylated poly-L-lysine, poly(L-Lys)Gal.
      
To evaluate the optimal parameters for efficient gene transfer into human hepatoma HepG2 cells by the DNA-poly(L-Lys)Gal complexes, the bacterial reporter genes lacZ and cat were used.
      
Nonviral Gene Transfer Techniques, in Methods in Molecular Biolog
      
The Dependence of the Efficiency of Gene Transfer with the Use of Lipoplexes Based on New Dicationic Lipids on Their Structure
      
Lipoplexes based on cholesterol derivatives of oligo(ethylene propylene imines) in gene transfer In vitro and In vivo
      
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The p53 gene is one of the most common targets for genetic abhormalities in human laryngocarcinoma. In this report, the ability of wild-type p53 gene to induce apoptosis of laryngocar-cinoma cell was examined. The wt-p53 gene recombinant retroviral vector was constructed and the PA3l7 packaging cell line producing virus established. The recipient cell lines of Hep2 (laryngocarci-noma) containing the abnormal p53 gene were trans fected in vitro with 1ml fresh retroviral stock pro-duced by the PA317. Southern-blot...

The p53 gene is one of the most common targets for genetic abhormalities in human laryngocarcinoma. In this report, the ability of wild-type p53 gene to induce apoptosis of laryngocar-cinoma cell was examined. The wt-p53 gene recombinant retroviral vector was constructed and the PA3l7 packaging cell line producing virus established. The recipient cell lines of Hep2 (laryngocarci-noma) containing the abnormal p53 gene were trans fected in vitro with 1ml fresh retroviral stock pro-duced by the PA317. Southern-blot and Northern-blot were performed using the probe (1. 7Kb p53 cDNA). Assays for in vitro growth characteristics were per formed. The result showed that introduc-tion of wt-p53 greatly suppressed in vitro cellular growth of the laryngocarcinoma cell line and identi-fied that the wt-p53 gene mediated the process of cell apoptosis. In conclusion, the retroviral vector-mediated wt-p53 gene transfer appeared to be able to induce the apoptosis in human laryngocarcinoma bearing multiple genetic lesions. The observation strongly suggests that inactivation of the p53 gene plays a significant role in the pathogenesis of laryngocarcinoma.

为探讨抗癌基因在肿瘤治疗中作用的可能性,以反转录病毒N2A为载体,将p53cDNA及顺式作用元件反向插入N2A两端LTR间的XhoⅠ位点,获得了野生型p53基因反转录病毒重组体。将该病毒重组体转染单向性包装细胞系ψ-2和双向性包装细胞系PA317)筛选后扩增有抗性的细胞克隆,用其产生的新鲜病毒颗粒感染p53基因表达异常的人喉癌细胞系(Hep2)。发现有前病毒整合的受体细胞,细胞染色质浓缩周边化,细胞肿胀,出现凋亡小体,基因组DNA电泳图谱呈阶梯状改变。结果表明反转录病毒介导的野生型p53基因能不同程度地诱导人喉癌细胞凋亡。

Purpose:To investigate the possibility of gene transfer into retina neural cells. Methods :pcDNA3-LacZ and pcDNA3-GDNF were transferred into rat retinal neural cells with cationic lipofectin. The gene transfer and expression of GDNF were detected by means of RT-PCR and SDS-PAGE.

目的:探讨胶质细胞源性神经生长因子(GDNF)基因转染培养的视网膜神经细胞的可能性及其表达情况。方法:培养的SD大鼠视网膜神经细胞应用脂质体法转染报道基因(pcDNA3-LacZ)和pcDNA3-GDNF,应用RT-PCR及SDS-PAGE电泳检测其表达情况。结果:pcDNA3-LacZ可转染到视网膜神经细胞。应用RT-PCR可在转染的培养细胞中检测到GDNF转录水平的表达,并可通过SDS-PAGE电泳观察到32KD的表达产物。结论:pcDNA3-LacZ可以作为视网膜神经细胞转基因成功与否的判别指标。pcDNA3-GDNF可转染培养的SD视网膜神经细胞,并可检测到其表达,为青光眼的视功能损害防治提供了一种新的可能。眼科学报1998;14:57—60。

ObjectiveTo compare different routes of administration on the ability of liposomes carrying an exogenous gene to introduce that gene into murine ocular tissues.MethodsPlasmid DNA(pGME 7Zf(+)) with beta galactosidase gene (DOSPER) was applied topically to eyes or injected into vitreous,tail vein,or retro orbital region of adult mice.Control and treated mice eyes were enucleated at 2?h,1?d and 2?d,1 week,2?and 4?weeks post injection ...

ObjectiveTo compare different routes of administration on the ability of liposomes carrying an exogenous gene to introduce that gene into murine ocular tissues.MethodsPlasmid DNA(pGME 7Zf(+)) with beta galactosidase gene (DOSPER) was applied topically to eyes or injected into vitreous,tail vein,or retro orbital region of adult mice.Control and treated mice eyes were enucleated at 2?h,1?d and 2?d,1 week,2?and 4?weeks post injection or topical application.Gene expression was detected by enzymatic color reaction using beta gal stain as a substrate in frozen sections of enucleated eyes.ResultsCell positive for beta galactosidase expression showed blue staining of enzymatic color reaction in tissue sections.Liposome mediated gene transfer was detected in murine cornea,iris,ciliary body and retinal ganglion cells,photoreceptors,and retinal pigment epithelium using all four routes of administration Injection into vitreous,tail vein and retro orbital region allowed transfer of the gene into the choroid.The gene could not be transferred into murine choroid by topical application.Gene expression in murine ocular tissues was observed after 1?d following injection or topical application.The expression lasted at least 1?month.Blue staining indicating an enzymatic color reaction was not observed in all of control eyes.ConclusionEfficient and stable transfer of functional genes could be achieved by liposome delivery into the cornea,iris,ciliary body,choroid and retina of mice.

目的 了解活体内不同途径应用脂质体转染外源基因在眼组织的分布情况。方法 将包有 β 半乳糖苷酶基因质粒的脂质体DOSPER ,分别采用玻璃体腔内注射、尾静脉注射、表面点药及球后注射到小鼠体内 ,2h ,1,2天、1,2 ,4周后用 β gal染色法观察 β 半乳糖苷酶基因在眼组织内的分布情况。 结果 玻璃体腔内注射、尾静脉注射、表面点药及球后注射 4组的小鼠眼球组织包括角膜、虹膜、睫状体、视网膜 ,均有 β 半乳糖苷酶基因表达 ,除表面点药组外 ,其他 3组小鼠脉络膜也有 β 半乳糖苷酶基因表达。用药 1天后开始出现 β 半乳糖苷酶基因阳性表达 ,持续达 1个月以上。 结论 脂质体能有效、稳定地转染外源基因到活体眼组织的角膜、虹膜、睫状体、脉络膜、视网膜。

 
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