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polio virus     
相关语句
  脊髓灰质炎病毒
     Results: The average reduction factor(log_(10)) of poliovirus in suspension exposed to chlorine dioxide 300 mg/L for 10 min,sodium hypochlorite containing available chlorine 400 mg/L for 20 min or sodium dichloroisocyanurate 800 mg/L for 10 min was(≥4.00.)
     结果,以含300 mg/L二氧化氯作用10 m in,含有效氯400 mg/L的次氯酸钠作用20 m in,含有效氯800 mg/L的二氯异氰尿酸钠作用10 m in,对悬液内脊髓灰质炎病毒平均杀灭对数值≥4.00。
短句来源
     Study on construction and expression of chimeric plasmid of HIV-1 CN54 strain gagprotease gene and Poliovirus cDNA
     HIV-I CN54株gagprotease基因嵌合脊髓灰质炎病毒cDNA质粒的构建和表达研究(英文)
短句来源
     Methods Analyze the transcription and expression levels of bcl-2 gene family (bcl-2, bax and bak) in human embryonic lung diploid cell KMB17 at different time after infection with poliovirus were analyzed by RT-PCR.
     方法 采用人胚肺二倍体细胞(KMB17),在脊髓灰质炎病毒(Poliovirus,PV)感染后不同时间经RT-PCR等方法分析凋亡过程中bcl-2基因家族(bcl-2、bax、bak)转录水平的表达情况。
短句来源
     〔Results〕 Poliovirus of 0.1~10 TCID 50 in water and of 10 TCID 50 in sewage could be detected with this method.
     〔结果〕能够检出 10 0 0ml水中的 0 .1~ 10TCID50 量的脊髓灰质炎病毒 ,在10 0 0ml预处理后的污水中加入 10TCID50 量的病毒 ,亦可以检出。
短句来源
     Confocal-FRAP measurements showed that 49.4 ±3.2% of the poliovirus plus-strand RNA molecules diffused freely (with a D value of 9.6 ±1.6×10~(-10) cm~2/s) within their distribution region and the rest 50.5 ±2.9% were almostimmobile and only moved very slowly with the change of RNA distribution region.
     荧光漂白恢复检测显示约有49.4 ±3.2%的脊髓灰质炎病毒正链RNA 在其分布区域内部可以自由扩散运动(扩散系数为9.6 ±1.6×10~(-10) cm~2/s),其余的50.5 ±2.9%在FRAP 测定期间几乎静止不动,而只是随着整个RNA 分布区域的变化缓慢移动。
短句来源
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  脊髓灰质炎
     AIM To observe the synergetic virucidal effect by low amperage direct currennt (LDC) and free chlorine (FC) for poliovirus type I (PV1) in water.
     目的 观察微电流 (low amperage direct current,LDC)与游离氯 (free chlorine,FC)对水中脊髓灰质炎 I型病毒 (PV1 )的协同灭活效果 .
短句来源
     25 strains were echovirus,1 strain was coxackievirus,1 strain was poliovirus Ⅰ.
     其中埃柯病毒25株,柯萨奇病毒1株,脊髓灰质炎(脊灰)病毒1株。
短句来源
     The Analysis on the Effect of the Poliovirus Vaccine Supplemental Immuniztion from 1993 to 1998 in Shandong Province
     山东省1993~1998年脊髓灰质炎疫苗强化免疫效果分析
短句来源
     ANALYSIS OF THE WILD TYPE POLIOVIRUS EPIDEMICSTRAINS IN HAINAN PROVINCE, 1985-1993
     海南省1985~1993年脊髓灰质炎流行野毒株的分析
短句来源
     Surveillance of Levels of Neutralizing Antibodies agalnst Poliovirus among Children Aged 0-3 in Rural Areas of Anhui Proviuce
     安徽省农村0~3岁儿童脊髓灰质炎中和抗体水平调查
短句来源
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  脊髓灰质炎病毒
     Results: The average reduction factor(log_(10)) of poliovirus in suspension exposed to chlorine dioxide 300 mg/L for 10 min,sodium hypochlorite containing available chlorine 400 mg/L for 20 min or sodium dichloroisocyanurate 800 mg/L for 10 min was(≥4.00.)
     结果,以含300 mg/L二氧化氯作用10 m in,含有效氯400 mg/L的次氯酸钠作用20 m in,含有效氯800 mg/L的二氯异氰尿酸钠作用10 m in,对悬液内脊髓灰质炎病毒平均杀灭对数值≥4.00。
短句来源
     Study on construction and expression of chimeric plasmid of HIV-1 CN54 strain gagprotease gene and Poliovirus cDNA
     HIV-I CN54株gagprotease基因嵌合脊髓灰质炎病毒cDNA质粒的构建和表达研究(英文)
短句来源
     Methods Analyze the transcription and expression levels of bcl-2 gene family (bcl-2, bax and bak) in human embryonic lung diploid cell KMB17 at different time after infection with poliovirus were analyzed by RT-PCR.
     方法 采用人胚肺二倍体细胞(KMB17),在脊髓灰质炎病毒(Poliovirus,PV)感染后不同时间经RT-PCR等方法分析凋亡过程中bcl-2基因家族(bcl-2、bax、bak)转录水平的表达情况。
短句来源
     〔Results〕 Poliovirus of 0.1~10 TCID 50 in water and of 10 TCID 50 in sewage could be detected with this method.
     〔结果〕能够检出 10 0 0ml水中的 0 .1~ 10TCID50 量的脊髓灰质炎病毒 ,在10 0 0ml预处理后的污水中加入 10TCID50 量的病毒 ,亦可以检出。
短句来源
     Confocal-FRAP measurements showed that 49.4 ±3.2% of the poliovirus plus-strand RNA molecules diffused freely (with a D value of 9.6 ±1.6×10~(-10) cm~2/s) within their distribution region and the rest 50.5 ±2.9% were almostimmobile and only moved very slowly with the change of RNA distribution region.
     荧光漂白恢复检测显示约有49.4 ±3.2%的脊髓灰质炎病毒正链RNA 在其分布区域内部可以自由扩散运动(扩散系数为9.6 ±1.6×10~(-10) cm~2/s),其余的50.5 ±2.9%在FRAP 测定期间几乎静止不动,而只是随着整个RNA 分布区域的变化缓慢移动。
短句来源
更多       
  脊灰病毒
     A total of 3 strains of poliovirus Ⅰ, 2 strains of poliovirus Ⅱ,4 strains of poliovirus Ⅲ, 1 strain of poliovirus Ⅰ+Ⅱ, 1 strain of poliovirus Ⅰ+Ⅲ and 1 strain of poliovirus Ⅱ+non polio enterovirus were isolated from the specimens.
     同步分离出脊灰病毒Ⅰ型3株,Ⅱ型2株,Ⅲ型4株,Ⅰ+Ⅱ型1株,Ⅰ+Ⅲ型1株,混合P2+E1株。
短句来源
     Methods We analyzed the epidemiological, virology and clinical features of AFP cases infected by EV in Hebei Province from 2001 to 2005 Results During 2001-2005, the average annual infection rate of Polio Virus (PV) in AFP cases was 97%, and that of Non-Polio Enterovirus(NPEV)was 100%.
     方法对河北省2001-2005年EV感染的AFP病例的流行病学、病毒学及临床特征进行分析。 结果AFP病例脊灰病毒(PV)感染率年平均为9·7%,非脊灰肠道病毒(NPEV)感染率年平均10·0%。
短句来源
     Results A tolal of2 957 stool specimens of AFP cases were surveillanced during 1995-2003,258 strains of poliovirus were isolated,the rate of isolation was 8.7%,and they were vaccine virus;
     结果1995-2003年共监测AFP病例粪便标本2957例,共分离出脊灰病毒(PV)258株,平均分离率为8·7%,经国家脊灰实验室型内鉴定均为疫苗株;
短句来源
     Poliovirus ds-cDNA of China I9 strain was ligated to plasmid pAT 153 using EcoRI linkers, Clones were screened and the cDNA of about 95 per cent of China I9 genome was obtained.
     将合成的脊灰病毒中I9株ds—cDNA片段重组到质粒pAT153上,获得了约占脊灰病毒中I9基因组95%以上的cDNA克隆。
短句来源
     In 2001,2002,and 2004 in Anhui Province,vaccine-variant polioviruses were isolated from 8 acute flaccid paralysis(AFP) cases and were 0.22%-0.99% sequence divergence from Sabin virus in VP1 region. 1 of which was vaccine-derived poliovirus(VDPV).
     安徽省在 2 0 0 1、2 0 0 2、2 0 0 4年急性弛缓性麻痹 (AFP)病例监测中 ,有 8例AFP病例粪便标本分离到疫苗变异脊髓灰质炎 (脊灰 )病毒 ,与脊灰疫苗 (Sabin)株相比 ,VP1 区核苷酸差异率为 0 2 2 %~ 0 99%,其中 1例为疫苗衍生脊灰病毒 (VDPV)。
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  polio virus
The persistently infected cells were resistant to homologous super-infection but supported polio virus replication to normal titres.
      
Polio virus type 1, was chosen as the challenge virus.
      
The major component was ECHO-9 virus, the minor component was polio virus type 2.
      
The authors studied the effect of polio virus upon the morphology of the cells obtained from subculture of the renal tissue (Macacus rhesus), heart (Macacus cynomolgus) and HeLa and HEp-2 malignant cells.
      
This phenomenon occurs with HeLa, KB, BHK-21 and L929 cells and EMC, SFV, VSV and Polio virus and is dependent on the ability of the virus to infect the cells.
      
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Sixty mouse hybridoma cell lines secreting antibodies against type 3333333 polio-viruses were established by fusion of a mouse myeloma cell line SP2/0 with spleen cells from mice immunized with Saukett and Zhong Ⅲ2 virus strains. Comparison of microneutralization test (MNT) , immunofluorescent test (IF) and ELISA for screening positive hybridoma cell lines was carried out. ELISA gives the highest positive rate(30.4% ) while the positive rates of MNT and IF are 15.6% and 11.6% respectively.Seventeen...

Sixty mouse hybridoma cell lines secreting antibodies against type 3333333 polio-viruses were established by fusion of a mouse myeloma cell line SP2/0 with spleen cells from mice immunized with Saukett and Zhong Ⅲ2 virus strains. Comparison of microneutralization test (MNT) , immunofluorescent test (IF) and ELISA for screening positive hybridoma cell lines was carried out. ELISA gives the highest positive rate(30.4% ) while the positive rates of MNT and IF are 15.6% and 11.6% respectively.Seventeen type Ⅲ wild polioviruses and Zhong Ⅲ2 as well as Saukett virus strains were tested with 15 McAbs against type Ⅲ poliovirus by cross-ELISA. Six "subtypes" of type Ⅲ poliovirus were proposed. They are vaccine, vaccine-like, intermediate, Saukett, Saukett-like and approximate Saukett strains.Among 11 hybridomas secreting McAb possessing virus-neutralizing activity, 2(C50, C36 ) neutralized Zhong Ⅲ2 vaccine virus and three vaccine-like strains in MNT. However, they did not neutralize Saukett and other 2 non-vaccine-like strains. So we suggest that McAb C50 and C36 may be valuable for distinguishing wild type Ⅲviruses from those of vaccine related strains.

在Ⅲ型Polio病毒McAb的研究中,用ELISA法筛选出60株阳性杂交瘤细胞系,其中11株中和试验阳性。ELISA试验型特异的McAb有10个,中和试验型特异的McAb有5个。McAb C_(36)和C_(50)只能与疫苗株和类疫苗株起中和反应而不能与非类疫苗株病毒起反应。因而这二株McAb具有区分Ⅲ型Polio野毒株与疫苗株相关病毒株的能力。另外应用McAb初步分析了17株Polio Ⅲ型病毒的抗原性。

Viruses in raw water at the inlet of the water plant and in tap water were detected by a modified Farrah's method (concentrating virus in water by two steps) together with the plaque assay. Virus recovery reached more than 90%.Viruses in the raw water of the water plant were 4-50% pfu/10L in different seasons. They were mostly enteroviruses, mainly nonvirulent strains of polio virus type I.During treatment in the water plant, 94% of viruses could be cleared by aluminium sulfate. The primary chlorination...

Viruses in raw water at the inlet of the water plant and in tap water were detected by a modified Farrah's method (concentrating virus in water by two steps) together with the plaque assay. Virus recovery reached more than 90%.Viruses in the raw water of the water plant were 4-50% pfu/10L in different seasons. They were mostly enteroviruses, mainly nonvirulent strains of polio virus type I.During treatment in the water plant, 94% of viruses could be cleared by aluminium sulfate. The primary chlorination procedure was the most important for inactivation of the viruses. 30 min after chlorination, 96% of viruses were killed. After 1 h, no virus could be further detected in water samples.No virus was discovered in 40 L of tap water at the supply system terminal.Thus, the tap water is believed to meet the 1971 WHO drinking water standard requirement of 0 pfu/10 L.

用改良Farrah法两次浓集病毒,并用BGM(Buffalo Green Monkey)细胞作空斑试验测病毒含量,对自来水和水厂原水中病毒进行检测,回收率达90%以上。水处理过程中,硫酸铝处理可以去除94%病毒。第一次加氯处理1/2 h后,可杀灭96%病毒,1h后不能再测到病毒。从管网末端自来水40L中,未能测到病毒,说明出厂自来水基本上已达到1971年世界卫生组织所订的饮用水国际标准中的病毒学指标——0pfu/10L。

About 40% municipal wastewater of Beijing city was converged into Gao-beidian wastewater system.For detecting the comtaminated viruses in the system, we adopted adsorption-elution technique with quartz powder to concentrate viruses fron the water samples.The recovery efficiency was 51-90%. Water samples collected from March to July 1985 were used for virus isolation and plaque assay. The results indicate that the contaminated viruses in the wastewater system were mostly enteroviruses...

About 40% municipal wastewater of Beijing city was converged into Gao-beidian wastewater system.For detecting the comtaminated viruses in the system, we adopted adsorption-elution technique with quartz powder to concentrate viruses fron the water samples.The recovery efficiency was 51-90%. Water samples collected from March to July 1985 were used for virus isolation and plaque assay. The results indicate that the contaminated viruses in the wastewater system were mostly enteroviruses with seasonal variations, polio-viruses mainly in spring,while mostly coxsackievirus B3 and non-enterovirus-es in summer.The concentration of viluses in vastewater was higher in the spring than in the summer. The average virus concentration in different waters were as follows: raw wastewater ( 58 pfu/1 ) , effluent of biological oxidation treatment ( <19, about 9 pfu/1), Hospital sewage ( <16, about 1 pfu /1 ) , ground water ( <2 pfu/1, undetectable ) .

本文报道用石英粉吸附——洗脱法浓集污水病毒,其回收率为51~90%。对1985年3~7月采集的不同水样进行病毒分离和蚀斑滴定,结果表明,高碑店污水系统中的病毒大部分为肠道病毒。春季以脊髓灰质炎病毒为主,夏季以柯赛奇B3及非肠道病毒为主。不同类型污水中的病毒浓度依次为:原污水58PFU/1;二级污水<19,约为9PFU/1;医用污水<16,约为1PFU/1,地下水<1.7PFU/1,未检出病毒。夏季水样内病毒浓度较春季低。

 
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