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western blot     
相关语句
  免疫印迹
     The expression of IKKα, IκBα, NF-κB P65 was examined by western blot and the NF-κB DNA binding activity was examined by electrophoretic mobility shift assay(EMSA) in laryngeal carcinoma tissue of 20 cases and normal laryngeal tissues of 10 cases.
     采用免疫印迹杂交法(Western blot)检测20例喉癌组织、10例正常组织的IKKα、IκBα、NF-κB P65的表达水平,以及应用凝胶电泳迁移率法(EMSA)测定NF-κB的DNA结合活性。
短句来源
     Methods Fluorometric reaction and Western blot test were used respectively to examine the double transfected stable expressions of CHO cell lines in mutant PS1 M146L and wild-type amyloid precursor protein 751(APP_(751)),the single transfected stable expression of SH-SY5Y cell line in mutant PS1V97L and PS1A136G,and theα-secretase activities and expression of sAPPPαin 3 types of PS1 mutation cells.
     方法利用荧光酶标反应法和Western blot免疫印迹方法分别检测突变型PS1M146L和野生型APP_(751)双转染稳定表达CHO细胞系,及突变型PS1V97L、PS1A136G单转染稳定表达SH-SY5Y细胞系,3种类型的PS1突变细胞α-分泌酶活性及其作用产物分泌型α淀粉样蛋白(sAPPα)表达。
短句来源
     Methods Total proteins and mRNA were extracted from NB4 cells treated with or without TSA with different doses (300,150,75,37.5,18.75 nmol/L) at various time points ( 4,8,12,24,48 h). By using Western blot,the levels of acetylated histone H3 and p21 WAF1/CIP1 protein expression were assayed. RT-PCR was used to detect the expression level of p21 WAF1/CIP1 mRNA.
     方法 应用 300、150、75、37 5、18 75 nmol/L浓度的TSA分别作用NB4细胞 4、8、12、24、48 h, 提取细胞的总 mRNA和总蛋白, 采用RT PCR技术检测 p21WAF1/CIP1mRNA表达情况, 并用免疫印迹技术 (Western blot) 观察组蛋白 H3 的乙酰化水平变化及 p21WAF1/CIP1蛋白的表达水平。
短句来源
     The positive rates of antibodies to 229E, OC43 and SARS-CoV were 97% , 99% , and 2% in the 100 serum specimens from healthy donors by Western blot; while the corresponding rates were 97% , 100% and 100% in the 34 convalescent sera from SARS patients, respectively.
     免疫印迹检测100例健康献血员血清中229E、OC43和SARS-CoV N蛋白IgG阳性率分别97%、99%和2%,34例SARS患者恢复期血清中229E、OC43和SARS-CoV N蛋白IgG阳性率分别97%、100%和100%;
短句来源
     METHODS: RLFs were isolated and cultured and then treated with 10 μg/L TGFβ1. The transcriptive activity of SP1, AP1 and Smad3-Smad4 and the expression of plasminogen activator inhibitorⅠ(PAI-1) and collagen Ⅰ were detected by means of electrophoretic mobility shift assay (EMSA), Western blot and immunohistochemical staining.
     方法:进行大鼠肺成纤维细胞的原代分离培养,应用10μg/L TGFβ1进行处理,通过凝胶阻滞电泳(EMSA)、免疫印迹和免疫组化染色等方法,检测照射后转录因子SP1、AP1和Smad3-Smad4活性的变化及Ⅰ型胶原和Ⅰ型组织纤溶酶原激活物抑制剂(plasminogen activator inhibi-torⅠ,PAI-1)表达的变化。
短句来源
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  western印迹
     10μmol/L and 20μmol/L lactacystin were chosen for treating SH-SY5Y cells,and the cellular polyubiquitinated proteins were detected by Western blot.
     选取10μmol/L和20μmol/L lactacystin处理SH-SY5Y细胞,Western印迹法检测细胞内多泛素化蛋白的含量。
短句来源
     Western blot method was used to detect the effects of TGF-β 1 on the expression of ERK 1 /ERK 2proteins.
     Western印迹法观察不同剂量TGF β1对ERK1/ERK2 蛋白表达的影响。
短句来源
     The mRNA and protein expressions of h/mTIMP-1, mTIMP-1, TIMP-2, TIMP-3, MMP-9, MMP-2, and COLⅣα5 mRNA were detected by Northern blot and Western blot.
     采用Northern杂交、Western印迹方法检测两组小鼠肾组织内h/mTIMP-1、mTIMP-1、TIMP-2、TIMP-3、MMP-9、MMP-2和COLⅣα5mRNA及蛋白质表达;
短句来源
     The expression of Kv1.3 and Kir2.1 channels were examined by immunocytochemistry, RT-PCR and Western blot.
     采用免疫细胞化学、RT-PCR和Western印迹检测人单核细胞源性巨噬细胞和泡沫细胞上Kv1.3和Kir2.1的表达。
短句来源
     RT-PCR, Western blot method were used to examine the expression changes of ICAM-1 and VCAM-1 after 12 weeks.
     用RT-PCR及Western印迹的方法检测12周后ICAM-1、VCAM-1表达的改变。
短句来源
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  蛋白印迹
     Western blot was used to detect Scurfin protein expression of CD4~+CD25~+ Tr cells.
     Western蛋白印迹法检测CD4+CD25+T细胞Scurfin蛋白表达。
短句来源
     Methods Wistar rats were exposed to EMP(6×10~4V·m~(-1)) and the expression of brain-derived neurotrophic factor(BDNF) and neural celladhesion molecule(NCAM) proteins in hippocampus were detected with Western blot analysis.
     方法以EMP 6×104V.m-1照射Wistar大鼠,用Western blot蛋白印迹法检测海马组织中脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)和神经细胞黏附分子(neural celladhesion molecule,NCAM)的表达。
短句来源
     RT-PCR,immunocytochemistry and Western blot analysis were applied to test the mRNA and protein expression of cyclin D1,cyclin B1 and Bcl-2 in C6/IL18 and C6 cells respectively.
     用RT-PCR,蛋白印迹、免疫细胞化学方法分析C6/IL-18细胞cyc lin D1,cyc lin B1,Bc l-2 mR-NA及蛋白的表达.
短句来源
     Methods Human colon adenocarcinoma cell line HCT116 was treated with different concentration of indomethacin for 24 h and CDK 2,CDK 4, Bcl 2, Bax and p21 WAF1/CIP1 protein were detected by Western blot.
     方法 不同浓度的吲哚美辛作用于结肠癌细胞株HCT116细胞 2 4h ,通过Western蛋白印迹技术检测CDK2 、CDK4 、p2 1WAF1/CIP1、Bcl 2及Bax蛋白表达。
短句来源
     Western blot was used to detect the expression of procaspase-3 protein in 3AO cells with transfection of pcDNA3-casp3 (3AO-pcDNA3-casp3) or pcDNA3 (3AO-pcDNA3).
     应用蛋白印迹法检测pcDNA3casp3和pcDNA3质粒转染后的3AO细胞(3AOpcDNA3casp3、3AOpcDNA3细胞)中procaspase3蛋白的表达情况;
短句来源
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  免疫印迹法
     The expression of acetyl histone H3 and H4,P300,P53 and c-myc protein were tested by western blot.
     蛋白免疫印迹法检测乙酰化组蛋白H3、H4、P300、P53、c-myc蛋白的表达。
短句来源
     METHODS The viability of PC12 cells was determined by MTT assay. The expression of HIF-1α and phospho-ERK1/2 (p-ERK1/2) were detected by Western blot. The expression of HIF-1α mRNA was detected by RT-PCR.
     方法MTT比色法观察PC12细胞活性,蛋白质免疫印迹法分析PC12细胞HIF-1α及磷酸化细胞外信号调节激酶1/2(p-ERK1/2)蛋白表达,RT-PCR观察HIF-1αmRNA表达。
短句来源
     Methods The phosphorylation of p44/p42MAPK and Akt were detected by Western blot. The role of MAPK and PI3K in the cellular action of PVC12 cells induced by NGF were assessed by use MAPK and PI3K specific inhibitor PD98059 and LY294002 respectively.
     方法应用蛋白免疫印迹法分析p44/p42 MAPK和Akt的磷酸化水平,并利用MAPK抑制剂PD98059和PI3K抑制剂LY294002,观察其对NGF诱导的PC12细胞形态学改变的影响。
短句来源
     The expression of IGF-IR in gastric cancer cells SGC7901and MGC803 were measured by western blot.
     此外,应用蛋白免疫印迹法(western blot)检测胃癌细胞系SGC7901,MGC803的IGF-IR的表达。
短句来源
     ⑤ Myocardial cell Bcl-2, Bax and p53 expression were assessed with Western blot.
     ⑤采用免疫印迹法检测心肌细胞Bcl-2,Bax及p53蛋白表达。
短句来源
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  western blot
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis revealed that T800/T1300 were highly expressed in the form of an inclusion body induced by isopropylthiogalactoside.
      
Western blot showed that the xooR gene was successfully expressed in vitro in E.coli as a XooR-GFP fusion protein with a size of 54 ku.
      
harveyi TS-628 strain isolated from infected groupers were extracted and Western blot analysis was used to detect the antigenicity of these extractions.
      
Results of the Western blot assay reveal that there are four positive flagellin bands: 35 kDa, 38 kDa, 43 kDa, and 52 kDa, of which the 43 kDa and 52 kDa bands displayed the strongest positive reaction.
      
HIF-1α protein was measured by Western blot method.
      
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In enzyme linked immunosorbent assay (ELISA) the key reagent is the enzyme-antibody conjugate which determines the specificity and sensitivity of this method. In this article the use of a heterobifunctional crosalinking reagent, N-su ccinimidyl 3- (2-pyridyldithio) propionate (SPDP) , for the conjugation of horseradish peroxidase (HRP) and rabbit anti-mouse IgG (rabbit IgG) was described .Different ratios of SPDP/HRP, SPDP/IgG and HRP/IgG were studied and the removal of free HRP and free IgG was investigated....

In enzyme linked immunosorbent assay (ELISA) the key reagent is the enzyme-antibody conjugate which determines the specificity and sensitivity of this method. In this article the use of a heterobifunctional crosalinking reagent, N-su ccinimidyl 3- (2-pyridyldithio) propionate (SPDP) , for the conjugation of horseradish peroxidase (HRP) and rabbit anti-mouse IgG (rabbit IgG) was described .Different ratios of SPDP/HRP, SPDP/IgG and HRP/IgG were studied and the removal of free HRP and free IgG was investigated. SDS-PAGE and Western Blot showed that the conjugate products contain no aggregate of either HRP or IgG.In ELISA our HRP-IgG conjugate preparation can be diluted down to 1:20, 000 (or more) when the concentration (A 230nm) of the conjugate is 1.0 and the absorbance of developed color (A 4921nm) produced from substrate and conjugate is 1.0.

酶标免疫测定法(ELISA)中最关键的化合物是酶-抗体结合物,将酶和抗体交联起来需用交联剂。本文作者使用了N-琥珀酰亚胺基3-(2-吡啶基二硫)丙酸酯(简称SPDP)将辣根过氧化物酶(HRP)和兔抗小鼠IgG(兔IgG)交联起来。我们试验了SPDP/HRP,SPDP/IgG和HRP/IgG的不同比例,以期获得活性高的酶-抗体结合物。此外还研究了从结合物中去除自由HRP和自由IgG的方法。用SDS-PAGE及硝酸纤维膜电泳转移法证明本法制备的结合物不含HRP及IgG的自身聚合物。用ELISA法鉴定结合物制品时,一般稀释度可达到1:10,000以上,有的可达到1:20,000(当结合物浓度A_(280nm)=1.0,底物显色A_(492nm)=1.0时)。

Leishmania donovani promastigote anti-gens recognized by McAbs were characterizedwith SDS-polyacrylamide electrophoresis,Western blot and ELISA methods. Promas-tigote antigens of L. donovani XinjiangStrain run in SDS-PAGE were separatedinto more than 60 bands, which were thentransferred through Western blot to nitrocel-lulose sheets. The specific antigen bandsrecognized by McAbs were identified on thesheets with ELISA. The McAbs (A-9, A-11, C-11, E-12 and H-10) prepared in ourlaboratory against L....

Leishmania donovani promastigote anti-gens recognized by McAbs were characterizedwith SDS-polyacrylamide electrophoresis,Western blot and ELISA methods. Promas-tigote antigens of L. donovani XinjiangStrain run in SDS-PAGE were separatedinto more than 60 bands, which were thentransferred through Western blot to nitrocel-lulose sheets. The specific antigen bandsrecognized by McAbs were identified on thesheets with ELISA. The McAbs (A-9, A-11, C-11, E-12 and H-10) prepared in ourlaboratory against L. donovani. XinjiangStrain bounded to 7 bands and these mole-cules ranged in M. W. from 41 to 77kilodaltons. Among the 5 McAbs, A-9 andC-11 appeared to recognize the same epi-tope as shown by the fact that the anti-gens recognized by A-9 were the same inM. W. as those by C-11, A-11, E-12 andH-10; each recognized some unique bandswhile they also bounded to common bands.

本文报道用SDS-PAGE、Western blot和ELISA方法,对杜氏利什曼原虫新疆株前鞭毛体中McAbs识别的抗原成分进行了检测。我们制备的抗杜氏利什曼原虫新疆株McAbs(A-9、A-11、C-11、E-12和H-10)共识别7条特异性抗原区带,抗原分子量分别为77000、74000、59000、54000、48000、43000和41000。

Sera from 18 Chinese haemophiliacs treated with factor Ⅷ produced in USA from 1982 to 1984 were tested for antibody to LAV/ H T L V- Ⅲ by ELISA. Four patients ( 22.2% ) showed strong positive results which were confirmed by immunofluorescence test and Western blot,but none of them has developed symptoms related to AIDS. Two Chinese haemophiliacs treated with locally produced factor Ⅷ was sero-negative.The first AIDS patient from abroad died in Beijing in 1985 was also sero-positive.In conclusion ,the data...

Sera from 18 Chinese haemophiliacs treated with factor Ⅷ produced in USA from 1982 to 1984 were tested for antibody to LAV/ H T L V- Ⅲ by ELISA. Four patients ( 22.2% ) showed strong positive results which were confirmed by immunofluorescence test and Western blot,but none of them has developed symptoms related to AIDS. Two Chinese haemophiliacs treated with locally produced factor Ⅷ was sero-negative.The first AIDS patient from abroad died in Beijing in 1985 was also sero-positive.In conclusion ,the data indicated that the Chinese haemophilics were exposed to LAV/HTLV-Ⅲ via imported factor Ⅷ from USA.

对浙江省1982~1984年注射了美国产浓缩Ⅶ因子制剂的18例血友病患者,用酶联免疫吸附法(ELTSA)检测了血清中淋巴腺病病毒/人T细胞Ⅲ型病毒(LAV/HTLV-Ⅲ)抗体,发现4例阳性,并经免疫荧光试验和Western印迹法证实。2例应用了国产浓缩Ⅷ因子者抗体阴性。一例从美国来华旅游死于艾滋病者,LAV/HTLV-Ⅲ抗体阳性。本研究证明,LAV/HTLVⅢ病毒巳通过美国生产的Ⅷ因子制剂传入中国。

 
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