The optimum conditions were reaction temperature 37℃, pH 4.5～5.5, the concentration of LA 0.1%, inoculation dosage 5%～7% and incubation time 24h. Under the optimum conditions the yield of CLA was 31.2%.
It was observed that at usage 50, 100, 200μg/ml of PHA, during 24, 40, 48, 72 h incubation time influence the production of IL-2. The results showed that suitable condition of lymphatic node cell was 200μg/ml PHA, spleen cell was 100μg/ml.
The results indicated that pH value in incubation solution (3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5 and 8.0), incubation temperature (25 ℃ and 37 ℃), incubation time (0.5, 1, 2, 5, 10, 20, 30 and 40 min) and the additional amount of BBMV did not affect hydrolysis of the dipeptide.
The transfection efficiency was 17.5% in condition as flow cell density 0.1×106 /mL, plasmid incubation time 30 min, plasmid and lipo-some incubation 15 min, plasmid and liposome ratio as 1 ∶10, transfection time 2 h, NAIR-1 as trans-fection culture medium.
The apoptosis rate was 94.0±14.4% incubated for 10 d in the 100 μmol/L Aβ25-35 group,and these differences were significant(P<0.05). The apoptosis rates increased with the Aβ25-35 concentrations and incubation time.
Experimental results under optimal conditions showed that dpm of ~3H-labelledsubstrate decomposed increased with the prolongation of incubation time when blood plasma andintestinal homogenate were incubated 1-5 hours and 0.25-5 hours respectively. The experimentalresults also showed that the decomposed ~3H-labelled substrate of intestinal homogenate diluted atdifferent concentrations decreased with increased dilution.
The COMT activity increases linearly with increase of incubation time up to 1 h and with increase of protein concentrations ranged from 1.7 to 6 mg·ml 1 respectively. The optimal concentration of magnesium chloride is ten times higher than that previously reported.
Methods:By applying mouse B cell line (HB95) as a model,a series of parameters for establishing a specific HLA Ⅰ antibody detecting ELISpot method were under construction,including the source of HLA antigen,the optimal concentration of coated antibody and the optimal incubation time of B cells. At the mean time,the sensitivity and specificity of this method were checked by using EBV transforming human B cell line to interfer with mouse B cell line.
Trehalose uptake was measured as a function of extracellular trehalose concentration, incubation time and incubation temperature. We found an effective uptake of the trehalose at 37 ℃ in a time span of 4 h in the presence of 50 mmol/L external trehalose. The internal trehalose concentration reached above 15 mmol/L.