The results indicated that LAT has advantages such as simple、rapid、sensitive、specific and detectable on site and so on,it is a new method that is suitable for grass-roots unit to detect serum antibodies against chicken NDV.
Positive numbers are 16 in manuforage by LAT. Positive samples are 18 in manuforage,The coincidence rate of both methods reached 83.8% in manuforage. The results showed that LAT has advantages such as simply,rapidly,sensitively,specifically and detects on site and to on.
The polymorphic fragments amplified by both primers were cloned and sequenced. The sequencing results showed that there was transition(T-C) at 645 site and(G-A)at 641 site,one transition(T-C)at 3 264 site of EX-FABP gene in chicken, respectively.
The fragment of Actinobacillus pleuropneumonia APXⅡ gene was cloned into the multiple cloning site of Eukytote expressing vector pET-28a,The recombinant of pET-APXⅡ was cultivated and induced with IPTG.
We synthesized gene fragment of the active site of the coagulation factor X by RT-PCR from the liver of the mice. We construct the expression vector pET28-HSP65-FXa by connect gene fragment with the HSP65gene fragment. The HSP65-FXa fusing protein is expressed in the E .
A in situ hybridization(ISH) method was used to detect low copy FMDV in infected cells,in order to illuminate FMDV multiplication site. In this study,FMDV RNA was located and detected in BHK-21 cells by ISH. Cells infected experimentally with O/Akesu/58(10~7TCID_(50)) at 2,4,6,8 and 10 hours post-inoculation.
Therefore, van der Waals' interactions between acetylcholinesterase and these drugs are stronger than those between butyrylcholinesterase probably due to a small active site gorge and a significant peripheral anionic site for acetylcholinesterase.
Novel 5-substituted esters and homologous ester and amido derivatives of 4,5 dihydro-3,3-diethyl-2(3H)-furanone were synthesized and evaluated for anticonvulsant activity in rodents and for affinity to a site on the GABAA receptor complex ([3H]TBOB).
The results showed that these compounds are capable of important interactions with the NNRT binding site, which encouraged us to submit them for biological assay.
From these data, it may be deduced that the administration of high concentration of 18-methyl norethindrone can displace ketoprofen from its secondary binding site.
This work attempts to calculate the binding-site number using fluorescence spectroscopic method with bovine serum albumin (BSA) and Indo-1 as protein and ligand models, respectively.