In vitro incubation of silk glands from larvae of day 6 of the last instar with 20_hydroxyecdysone showed an accelerated apoptosis as compared with those in controls, indicating that the pulses of 20_hydroxyecdysone before larval_pupal metamorphosis trigger genetic regulatory hierarchies that mediate apoptosis and hydrolysis.
Sperm samples exposed to PF for 15 min at (0mM, 1mM, 5mM and 10 mM) had similar (P>0.05) sperm motility, progressive status, viability and acrosomal integrity patterns throughout the 90 min examination period. Long-term incubation with 1 mM PF, did not influence motility among time periods (P>0.05).
Additionally, sperm incubated with 10 mM PF had fewer (P<0.05) intact acrosomes (56.3 ± 2.8) after 4 hrs incubation, compared with controls (71.0 ± 3.3%). These two studies of giant panda sperm demonstrated that giant panda sperm that are cooled and cryopreserved rapidly in pellet form, have better survival following slow rate thaw. Further, giant panda sperm are sensitive to the common motility enhancer PF.
After 24h incubation, cellular DNA was isolated and analyzed for BP-derived DNA adducts by 32P-postlabeling technique.
Cell incubation experiments show that PLA-PU has biocompatibility comparable to that of pure PLA.
We studied the effects of nitrogen source, initial pH, temperature, incubation time, medium composition, and surfactants on cellulase production.
The only known Early Cretaceous bird embryo fossil has shown that precocial birds had occurred prior to altricial birds in avian history, and the size of the embryo and other analysis indicate it probably had a short incubation period.
Individuals of the species in pair-banding and nest-detecting periods have larger home ranges than those in incubation and rearing periods.