Under each double factor condition the activity of AKP and the accumulated absorption of Leu were tested and the results show that the optimum condition for intestinal incubation in vitro of 1 year old grass carp is: culture temperature 25℃, culture time 5 min, culture medium Yamamoto solution (NaCl 0.75%, KCl 0.02%, NaHCO 3 0.002%).
However, the appearance of DNA ladder, which is a biochemical hallmark of apoptosis, did notoccur during cold incubation until rewarming the cells to 32. It could be concluded that low temperature (10) could induce apoptosis in Colossoma Brachypomum CBT cells.
When eggs were laid by different means,the dripping water incubation rate(94.7±2.1)% was greatly higher than the static water incubation rate(26.7±7.7)%(P<0.01),and fries came out slower while their yelk disappeared faster in dripping water.
After 24h incubation, cellular DNA was isolated and analyzed for BP-derived DNA adducts by 32P-postlabeling technique.
Cell incubation experiments show that PLA-PU has biocompatibility comparable to that of pure PLA.
We studied the effects of nitrogen source, initial pH, temperature, incubation time, medium composition, and surfactants on cellulase production.
The only known Early Cretaceous bird embryo fossil has shown that precocial birds had occurred prior to altricial birds in avian history, and the size of the embryo and other analysis indicate it probably had a short incubation period.
Individuals of the species in pair-banding and nest-detecting periods have larger home ranges than those in incubation and rearing periods.