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incubation
相关语句
  孵育
    One Step Incubation e ELISA in a Single Assay Using Monoclonal Reagents for the Detection of Both HBeAg and Anti-HBe
    一孔一期孵育法同时检测HBeAg与抗-HBe的单克隆试剂酶免疫测定
短句来源
    THE RELATIONSHIP BETWEENACROSOME REACTION OF HUMAN SPERMATOZOA AND INCUBATION TIME
    人精子顶体反应与孵育时间的关系
短句来源
    RESULTS When incubation temperature was 25℃ and 37℃, PP were (28.0±1.4)% and (44.0±3.3)%, respectively, which were significantly higher than that at 4℃.
    结果25℃及37℃孵育条件时,PP分别为(28.0±1.4)%和(44.0±3.3)%,显著高于4℃的结果;
短句来源
    When incubation was controlled at 25-37℃ for 1.5-2.0 h, the measurement could be repeated very well.
    25~37℃孵育1.5~2.0h是合适的孵育条件。
短句来源
    HSF and HSC-T6 in logarithmic growth phase were incubated in culture plates in which the cultured cell density were adjusted to 1 x lOVml. OD values of the cells were tested by the method of MTT after 24hr incubation.
    取对数生长期培养细胞HSF,HSC一T6,调整细胞密度为lxl05个/m1,接种于%孔板24小时后,分别加入不同浓度pHGF,孵育24小时,进行MTT试验,检测55Onm0D值。
短句来源
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  “incubation”译为未确定词的双语例句
    Chemiluminescence Single Incubation Multilayer Immune Technique Detection of Rotavirus in Excrement
    化学发光单孵多层免疫技术检测粪样中轮状病毒
短句来源
    Results Real time RT-PCR indicated that,after 12 hours incubation,both 100 μg/L and 1mg/L LPS were able to elevate the levels of SSeCKS mRNA compared with control group,while 1mg/L LPS did not have a stronger effect than 100 μg/L.
    结果Real time RT-PCR显示,LPS可以上调SSeCKS mRNA水平,在作用浓度为100μg/L和1 mg/L时与对照组有显著差异(P<0.01)。
短句来源
    At the dose of 8.0μmol/L,the inhibitory effect on the T cell proliferation was en- hanced with incubation elongated from 24h to 96h.
    选择最佳剂量8.0μmol/L,SP600125对T细胞增殖的抑制作用随时间从24h递增至96b而逐渐增强;
短句来源
    CIK(cytokine-induced killer cells) are generated by incubation of peripheral blood lymphocytes of health adult or patient with anti-CD3 monoclonel antibody (CD3mAb),interleukin-2 (IL-2),interleukin-1 (IL-1),and interferon-γ(IFN-γ). CIK cells have high proliferation and cytotoxic activity.
    正常人或患者外周血、骨髓单个核细胞中定期加入IFN-γ、IL-1、IL-2、CD3mAb经2-4W的诱导可获得大量的新型肿瘤杀伤细胞—细胞因子诱导的杀伤细胞CIK,此细胞增殖旺盛杀伤活性高。
短句来源
    ③Wortmannin+EP group(W+EP group):Wortmannin(100 nmol/L),a specific phosphatidylinositol-3-kinase(PI3-K)inhibitor,was added at 30 min before EP incubation;
    ③Wortmannin+EP组(W+EP)在EP预处理前30min予3-磷脂酰肌醇激酶(phosphatidylinositol-3-kinase,PI3-K)特异性阻断剂Wortmannin(100nmol/L);
短句来源
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  incubation
After 24h incubation, cellular DNA was isolated and analyzed for BP-derived DNA adducts by 32P-postlabeling technique.
      
Cell incubation experiments show that PLA-PU has biocompatibility comparable to that of pure PLA.
      
We studied the effects of nitrogen source, initial pH, temperature, incubation time, medium composition, and surfactants on cellulase production.
      
The only known Early Cretaceous bird embryo fossil has shown that precocial birds had occurred prior to altricial birds in avian history, and the size of the embryo and other analysis indicate it probably had a short incubation period.
      
Individuals of the species in pair-banding and nest-detecting periods have larger home ranges than those in incubation and rearing periods.
      
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A procedure for phenotyping hyperlipoproteinemia is presented. 0.1 ml of a saturated solution of Sudan Black-B in petroleum ether-alcohol solvent (1:4) and 0.05 ml absolute ethyl alcohol was added to 1 ml of serum. After incubation for 20 min at 37℃ and removal of excess dye by centrifuging, 0.04 ml of the stained serum was applied to filter paper for electrophoretic separation. The optimal conditions for paper electrophoresis were an albuminated "tris" buffer, pH 8.9, ionic strength 0.42 M in terms of...

A procedure for phenotyping hyperlipoproteinemia is presented. 0.1 ml of a saturated solution of Sudan Black-B in petroleum ether-alcohol solvent (1:4) and 0.05 ml absolute ethyl alcohol was added to 1 ml of serum. After incubation for 20 min at 37℃ and removal of excess dye by centrifuging, 0.04 ml of the stained serum was applied to filter paper for electrophoretic separation. The optimal conditions for paper electrophoresis were an albuminated "tris" buffer, pH 8.9, ionic strength 0.42 M in terms of tris with EDTA and boric acid concentrations at 0.017 M and 0.061 M respectively; potential gradient 5~6 volts/cm and a current of 0.75~1 mA/strip.

近年来冠心病的发病率和死亡率很高,青中年人中发病率亦日趋增多,因此冠心病成为现代医学研究的重大课题。作者初步建立了一个重复性较好,操作简便,费用经济,适用于临床实验室的脂蛋白纸上电泳预染分型法.以便为高脂蛋白血症分型提供较多的线索。一、用苏丹黑B石油醚-无水乙醇饱和溶液先染血清,后在含1%白蛋白的TEB(三羟甲基氯基甲垸-乙二胺四乙酸-硼酸)缓冲液中,电压160V,电流0.75~1.O毫安/滤纸条,电泳16小时,能使40微升正常血清在滤纸上出现除原点外还有三条着色区带,即β-脂蛋白,前β-脂蛋白及α-脂蛋白。正常人脂餐后,在原点与β-脂蛋白间还有乳糜微粒及前β-脂蛋白“拖尾”区带出现。二、用门诊数十名患者血清,分别电泳20次观察方法的重复性。结果:β-,前β-及α-脂蛋白相应地为43.O±O.42(S.E.)%,33.7±0.52(S.E.)%及23.4±0.47(S.E.)%。三、测定了33名(男18名,女15名),平均年龄为43.7岁,一般健康良好,无脂类代谢疾病的本医学院教职工的脂蛋白:α-,β-和前β-脂蛋白相应为187±42毫克/100毫升,241.3±34.2毫克/100毫升及75.2±29.3毫...

近年来冠心病的发病率和死亡率很高,青中年人中发病率亦日趋增多,因此冠心病成为现代医学研究的重大课题。作者初步建立了一个重复性较好,操作简便,费用经济,适用于临床实验室的脂蛋白纸上电泳预染分型法.以便为高脂蛋白血症分型提供较多的线索。一、用苏丹黑B石油醚-无水乙醇饱和溶液先染血清,后在含1%白蛋白的TEB(三羟甲基氯基甲垸-乙二胺四乙酸-硼酸)缓冲液中,电压160V,电流0.75~1.O毫安/滤纸条,电泳16小时,能使40微升正常血清在滤纸上出现除原点外还有三条着色区带,即β-脂蛋白,前β-脂蛋白及α-脂蛋白。正常人脂餐后,在原点与β-脂蛋白间还有乳糜微粒及前β-脂蛋白“拖尾”区带出现。二、用门诊数十名患者血清,分别电泳20次观察方法的重复性。结果:β-,前β-及α-脂蛋白相应地为43.O±O.42(S.E.)%,33.7±0.52(S.E.)%及23.4±0.47(S.E.)%。三、测定了33名(男18名,女15名),平均年龄为43.7岁,一般健康良好,无脂类代谢疾病的本医学院教职工的脂蛋白:α-,β-和前β-脂蛋白相应为187±42毫克/100毫升,241.3±34.2毫克/100毫升及75.2±29.3毫克/100毫升。四、讨论了不同类型高脂蛋白血症中脂蛋白的异常及分型在临床应用上的意义。

In this paper, we report for thefirst time the mediation of cytotoxicimmune reactions against human livercancer cells in vitro by xenogeneic "im-mune" RNA. The human liver cancercell strain (BEL-7402) employed wasestablished in tissue culture in ourlaboratory by explant from a surgicalspecimen. The cell-mediated assayusing ~(125)I-iododeoxyuridine (~(125)IUdR)-labeled target cells was used for thestudies. Normal, nonimmune humanperipheral blood lymphocytes were con-verted into "killer cells", which effectedimmune...

In this paper, we report for thefirst time the mediation of cytotoxicimmune reactions against human livercancer cells in vitro by xenogeneic "im-mune" RNA. The human liver cancercell strain (BEL-7402) employed wasestablished in tissue culture in ourlaboratory by explant from a surgicalspecimen. The cell-mediated assayusing ~(125)I-iododeoxyuridine (~(125)IUdR)-labeled target cells was used for thestudies. Normal, nonimmune humanperipheral blood lymphocytes were con-verted into "killer cells", which effectedimmune cytolysis of human liver cancercells, by incubation with "immune"RNA extracted from the lymph nodesand spleens of sheep which had pre-viously been immunized with the hu-man liver cancer cells being studied.It was observed that the cytotoxicityindex of lymphocytes increased signi-ficantly after incubation with "immune"RNA in ten experiments. The controlRNA from sheep non-specifically im-munized with complete Freund's adju-vent did not enhance cytotoxicity indexof lymphocytes except in one experi-ment. The immune response mediatedby the "immune" RNA was abrogatedwhen the "immune" RNA was treatedwith RNase prior to incubation withlymphocytes. Treatment with DNaseor pronase did not effect the activityof the "immune" RNA. From thesestudies, it was demonstrated that thexenogeneic "immune" RNA couldtransfer the cell-mediated immunitydirected against the surface antigenson the human liver cancer cells tonormal human lymphocytes. Thus, itwould be suggested that xenogeneic"immune" RNA might be consideredas an approach to immunotherapy forhuman hepatocarcinoma.

从人肝癌细胞(BEL-7402)高度免疫过的绵羊的淋巴器官中抽提的肝癌“免疫”RNA,和正常人外周血淋巴细胞温育后,在体外能介导肝癌细胞的免疫细胞溶解。用5-~(125)碘-2′-脱氧尿嘧啶核苷标记人肝癌靶细胞的微量细胞毒性试验进行测定时观察到,正常人淋巴细胞经肝癌“免疫”RNA处理后,其细胞毒性指数在16次实验中有10次有显著增高,但是,在与对照RNA温育后其细胞毒性指数(除一次实验外)均未出现有显著意义的变化。肝癌“免疫”RNA在用RNA酶降解后,传递细胞毒性免疫反应的活性即消失,而用DNA酶或灰链霉菌蛋白酶处理,却仍保持这种传递能力。由此证明,异种“免疫”RNA能够把针对人肝癌细胞表面抗原的细胞免疫性传递给正常人淋巴细胞。

In the course of preparation of lymphokines from human peripheral blood lymphocytes,a slow-migrating S component was found among the alkaline lymphokines that migrated towards the cathode in agarose gel electro-phoresis at pH 8.6, This component showed a chemotactic activity for PMN leukocytes and the activity was proved to be not associated with complements or other serum proteins and was able to be abolished by preincubation with anti-S component antiserum. The quantity of an alkaline protein increased markedly...

In the course of preparation of lymphokines from human peripheral blood lymphocytes,a slow-migrating S component was found among the alkaline lymphokines that migrated towards the cathode in agarose gel electro-phoresis at pH 8.6, This component showed a chemotactic activity for PMN leukocytes and the activity was proved to be not associated with complements or other serum proteins and was able to be abolished by preincubation with anti-S component antiserum. The quantity of an alkaline protein increased markedly after the incubation of heparinized human blood with PHA for 6 hours. This alkaline protein was then proved to be identical with S component and the B band of a QAE-Sephadex A-50 unadsorb-ed peak at pH 8.7 on line-immunoelectrophoresis. Some physico-chemical properties of the alkaline protein was also investigated. The optimum pH for its chemotactic activity is 7.6-8.1. The pI is about 9.4 and the molecular weight is 27,000d. Urea showed no apparent effect on this chemotaxis.

正常人外周血淋巴因子经硫酸铵盐析、凝胶过滤和离子交换层析后,得到一种具有中性多核白细胞趋化活性的碱性蛋白质。此蛋白质与在pH8.6琼脂糖电泳中泳向负极的S成份、SDSPAGE的B区带以及全血经PHA激活后含量升高的碱性蛋白具有相同的抗原性,而与补体或其它正常血清成份无关。其分子量约为27,000d,等电点为pH9.4。趋化活性的最适pH为7.6~8.1。

 
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