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tumor
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  肿瘤
    Efficient Induction of Antitumor Immunity by Exosomes Derived from Heat-shocked Tumor Cells
    应用热休克肿瘤细胞来源的EXOSOMES高效诱导抗肿瘤免疫应答反应及其机制的研究
短句来源
    Study of the Effect of Genetic Immunization with Xenogeneic TGF-β on the Efficacy of DNA Vaccine for Tumor
    异种TGF-β免疫对肿瘤基因疫苗免疫效能的影响研究
短句来源
    Development of Tumor Vaccine of EL-4 Cells Modified by GM-CSF Gene and B7.1 Gene
    GM-CSF和B7.1双基因修饰的EL-4细胞肿瘤疫苗的研制
短句来源
    The Evaluation of Gene Gun-mediated DNA Vaccine pWRG-neu Immunization Inhibited Neu-overexpressed Tumor Growth and Metastasis
    基因枪介导的neu基因DNA疫苗抗肿瘤作用的实验研究
短句来源
    STUDIES ON THE CHROMOSOMES OF TUMOR CELLS IN COMMON LABORATORY ANIMALS Ⅰ. KARYOTYPE ANALYSIS AND BANDING PATTERNS OF MOUSE ASCITES SARCOMA 180
    常用实验动物肿瘤细胞染色体的研究——Ⅰ小鼠肉瘤180(腹水型)核型及带型分析
短句来源
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  肿块
    A Comparison Between B-mode Ultrasound and Molybdenum Anode X-ray Radiograph in Diagnosing Breast Tumor
    B超与X线钼靶在乳腺肿块诊断中作用之比较
短句来源
    The efficacy rate of TAL from ascites treated with sold tumor was 40%(vein injection 14.2%,local injection 62.5%).
    另外发现胸腹水TAL对实质性肿块治疗疗效较低,为40%,其中静脉滴注更差,为14.2%,而介入注射与局部治疗较好,为62.5%;
短句来源
    (3) c-erbB-2 amplification was not associated with patients′age、gender、tumor size、location、differentiated type、lymph node metastasis.
    (3)胃癌组织中c-erbB-2原癌基因扩增与患者年龄、性别、肿块位置、肿块大小、分化程度、淋巴结转移无显著相关性。
短句来源
    Sixty four cases of breast tumor were diagnosed by both B-mode ultrasound and molybdenum anode X-ray radiograph and then confirmed by operation and pathological examination.
    64例经病理证实的乳腺肿块术前分别用B超与x线钼靶摄片进行诊断。
短句来源
    In this report,the RAPD technique has been applied to analyze the niddle boipsy specimens from different tumor patients.
    应用RAPD技术分析疑肿瘤病人临床肿块穿刺标本。
短句来源
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  恶性肿瘤
    A Study on T Lymphocyte Subpopulation,Soluble Interleukin - 2 Receptor Level and Erythrocyte Immune Function in Elderly Patients With Malignant Tumor
    老年恶性肿瘤患者红细胞免疫功能、T细胞亚群和可溶性白介素—2受体的研究
短句来源
    Contrast and Analysis on the Content of Cu, Zn, Ca, Mg, Se and the Ratio of Cu/Zn in the Serum of both the Patients with Malignant Tumor and the Healthy Subjects
    恶性肿瘤患者与健康人血清Cu、Zn、Ca、Mg、Se含量及Cu/Zn比值的对比分析
短句来源
    Dynamic Research of Serum SIL-2R-level Expressions of Malignant Gastroenteric Tumor Patients
    胃肠道恶性肿瘤血清SIL-2R水平表达的动态研究
短句来源
    The Significance of the Detection of T Lymphocyte Subsets and soluble Interleukin-2 in Patients with Malignant Tumor
    恶性肿瘤患者T细胞亚群和可溶性白介素-2受体检测及意义
短句来源
    Study on the immunological function of tumor patients before and after radiotherapy
    放射治疗前后恶性肿瘤患者免疫机能的研究
短句来源
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  “tumor”译为未确定词的双语例句
    Preparation of Anti Human Tumor Necrosis Factor α Single-Chain Variable Fragment Antibody by Phage Surface Display Technique and Its Humanization
    利用噬菌体表面展示技术制备抗人肿瘤坏死因子α单链抗体及其人源化改造
短句来源
    1、Identification of the RNA Chaperone Activity of Human Tumor Necrosis Factor Alpha in Vitro 2、Study on the Role of Argonaute Protein in miRNA Processs
    1、重组人TNF-α具有体外RNA伴侣分子活性研究 2、Argonaute蛋白参与miRNA作用过程的初步研究
短句来源
    Tumor Necrosis Factor-Inducible cIAP_2 Protein Inhibits Hepatitis B Virus Replication
    肿瘤坏死因子α诱导的cIAP_2蛋白抑制乙型肝炎病毒复制的研究
短句来源
    A Study on the Expression and Secretion of Tumor Necrosis Factor and Its High Activity Mutant
    人肿瘤坏死因子在大肠杆菌中高效表达及其高活性突变体的研究
短句来源
    T-DNA Transmision and Tumor Inducing Effect of Agrobacterium tumefaciens on Solatium nigrum
    致瘤农杆菌对龙葵的致瘤作用及其T-DNA的转移
短句来源
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  tumor
Seven compounds caused 50% growth inhibition (GI50) of tumor cells at concentrations of >amp;lt;100 μM while the remaining ten were not cytotoxic.
      
With regard to sensitivity, compounds 8f and 9c, f have proved to possess a remarkable activity against leukemia tumor cell lines (GI50?=?3.43-5.03?μM).
      
All newly synthesized compounds were evaluated for their antiproliferative activities against human lung tumor cell lines (A 549).
      
Analysis for a free boundary problem modeling tumor therapy
      
Tumor necrosis factor (TNF)-α-converting enzyme (TACE) is the major protease responsible for processing pro-TNF-α from membrane-anchored precursors to secreted TNF-α.
      
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The relation between Crabtree effect and protein biosynthesis has been studied in Ehrlich ascites tumor cells. It has been found that the addition of succinate to washed tumor cells, which had a low level of endogenous respiration, caused marked increase both in the rate of respiration and the rate of incorporation of C~(14)-labeled glycine into cellular protein. Thus active protein biosynthesis could be maintained solely on energy released in the oxidative metabolism of succinate, a fact not supporting...

The relation between Crabtree effect and protein biosynthesis has been studied in Ehrlich ascites tumor cells. It has been found that the addition of succinate to washed tumor cells, which had a low level of endogenous respiration, caused marked increase both in the rate of respiration and the rate of incorporation of C~(14)-labeled glycine into cellular protein. Thus active protein biosynthesis could be maintained solely on energy released in the oxidative metabolism of succinate, a fact not supporting the respirationdamage theory of tumor cells suggested by Warburg. When glucose or its analog, 2-deoxyglucose, was added together with succinate, both the rate of respiration and the rate of incorporation of C~(14)-labeled glycine were affected, the latter, however was much more markedly inhibited than the former. This indicates that under these conditions energy required for protein biosynthesis may not be so readily available, probably through the compartmentation of ATP within the mitochondria as suggested by a number of workers.

我們利用同位素C~(14)-标記甘氨酸参入蛋白貭的作用研究艾氏腹水肿瘤細胞中的Crabtree效应,观察由氧化作用释放出的能量被利用的情况。实驗結果說明艾氏腹水肿瘤細胞的呼吸效能是有效的,由琥珀酸氧化供应的能量可以支持旺盛的蛋白貭生物合成。說明由氧化磷酸化作用生成的ATP可以透到綫粒体外以供应蛋白貭生物合成的需要。若在加入琥珀酸同时加入葡萄糖或2-脫氧葡萄糖而引起Crabtree效应后,肿瘤細胞的呼吸略有降低,但是C~(14)标記甘氨酸参入蛋白貭的速度却比单独加入琥珀酸时要小得多,而仅接近单独加入葡萄糖时的参入速度,我們认为这可能是由于在引起Crabtree效应的条件下产生了线粒体中ATP局部化的緣故。

Activities of protein disulfide reductase were first shown by Nickerson and Falcone in yeast and later on by Hatch and Turner in germinating seeds of peas. Similar enzyme activities have been observed in mammalian tissues in our preliminary studies.In the present paper some results on the partial purification and properties of protein disulfide reductase in regenerating rat liver are reported.The enzyme activity was determined by measuring the increased amount of protein-SH groups formed from a protein fraction...

Activities of protein disulfide reductase were first shown by Nickerson and Falcone in yeast and later on by Hatch and Turner in germinating seeds of peas. Similar enzyme activities have been observed in mammalian tissues in our preliminary studies.In the present paper some results on the partial purification and properties of protein disulfide reductase in regenerating rat liver are reported.The enzyme activity was determined by measuring the increased amount of protein-SH groups formed from a protein fraction prepared from rat liver used as substrate.Protein-SH groups were estimated by a modified amperometric titration technique.By means of ammonium sulfate fractionation, followed by DEAE-cellulose adsorption and eluation, the enzyme was purified approximately 200 fold and separated completely from the activity of glutathione reductase. The enzyme preparation requires NADPH or NADH for its activity. The optimum pH is about 7.5. It is strongly inhibited by Zn~(++), Cu~(++), Hg~(++), iodoacetic acid and p-chloromercuribenzoate in a final concentration of 10~(-5)M. Reduced glutathione has no stimulatory effect on the enzyme activity. Besides oxidized glutathione, the purified enzyme preparation is also inactive towards some other disulfide compounds of low molecular weight such as lipoic acid and cystine. It is also inactive towards some crystalline proteins such as insulin, ribonuclease, bovin serum albumin and lysozyme. However, a low activity was noticed with bovin serum albumin as substrate when a boiled extract of the liver protein was added.Higher enzyme activities were found in some proliferating tissues such as regenerating liver, liver tumor and mouse embryo.

本文报告在大鼠组织中存在有促使肝蛋白—S—S—还原为蛋白—SH的酶活力,而以再生肝、肝癌、小鼠胚胎等组织的活力为高。利用硫酸铵分步沉淀及DEAE-纤维素吸附等法,可将再生肝中酶活力提纯约200倍,并可与谷胱甘肽还原酶活力完全分开。酶活力需要NADPH或NADH作为供氢体。最适pH为7.5。受Cu~(++)、Zn~(++)、Hg~(++)、对氯汞苯甲酸、一代碘乙酸等物质抑制。谷胱甘肽对酶活力无激活作用。提纯酶制剂不含有还原小分子—S—S—化合物的活力,对一些含—S—S—链的结晶蛋白质也无活力。

Cultivated cells taken from the cell strain of human tumor origin (clone 515, fromOsteochondrosarcoma) were studied under living condition with inverted phase contrastmicroscope. The generation time and the interphasic duration and mitotic phases of thecells were determined in the living state. The variations of the nucleoli and nucleolarassociated heterochromatin were observed with Feulgen and light green staining. Thenucleoli of daughter cells appeared gradually but their number decreased and their sizeincreased...

Cultivated cells taken from the cell strain of human tumor origin (clone 515, fromOsteochondrosarcoma) were studied under living condition with inverted phase contrastmicroscope. The generation time and the interphasic duration and mitotic phases of thecells were determined in the living state. The variations of the nucleoli and nucleolarassociated heterochromatin were observed with Feulgen and light green staining. Thenucleoli of daughter cells appeared gradually but their number decreased and their sizeincreased in the course of the interphase ages. This latter change is most likely due to fu-sion of the nucleoli. The nucleolar associated heterochromatin also varied in differentstages of interphase. It appeared as dots, a thin layer, or a cresent around the nucleolusat the early interphasic stage. At the late stage, the heterochromatin increased inamount and formed an uneven broad ring around the nucleolus or it might cover thewhole nucleolus. The distribution of alkaline phosphatase in the nudeolar system consisting of nucleo-lus and associated heterochromatin was in agreement with that of the DNA. In theearly stage of the interphase the small nucleoli were rich in alkaline phosphatase. Cells, under the aged cultivation, without changing the cultivating medium for 7 to10 days or cells in Hanks salt solution without any other nutrition were also studied.Under these conditions, there was no mitotic figure found. The nucleoli were round inappearance and varied in size. There was less amount of nucleolar associated hetero-chromatin less and it usually appeared as dots or as a thin layer around the nucleolus. In cells irradiated with 1000r of Co~(60), the nucleoli were enlarged and lobulated im-mediately after radiation. Each lobule was covered with a layer of chromatin. Before theonset of mitotic burst which took place two days after radiation the nucleolar hetero-chromatin was increased. On the 6th day after radiation, mitotic figures were dimini-shed, nucleoli decreased in size and the heterochromatin also appeared to be scanty.It seems that the variations of nucleolar associated heterochromatin is closely related tothe cell division. The site of protein synthesis is indicated by autoradiography with C~(14)-glycine. Tractsof radiation demonstrated by reduced silver particles appeared first near the peripheral re-gion of nucleolus, then the inside of the nucleolus also showed the appearance of silverparticles as the cooperated time prolonged. The amount of tracts increased as the hetero-chromatin increased. Thus the nucleolar system not only disappeared and reappeared in the mitotic cycle,but also varied in the course of the interphase age, being less significant after mitosis andmore so before mitosis. It was therefore closely related to cell division.

利用长期培养的人肿瘤细胞株,在相差显微镜的连续观察下,测定细胞的分裂时间、一代时间以及其在间期所处的时间。并用Feulgen和May-Grunwald Giemsa分别染色的材料,观察分裂间期各阶段的核仁与核仁周异染色质的变化。细胞分裂后子细胞内出现数量较多的核仁,以后数量逐渐减少,而体积则逐渐增大,并有合并趋势。核仁周异染色貭随分裂后间期时间的长短而有变化。核仁周的Feulgen阳性反应物貭从形成薄层、颗粒状或半圈形,增多到核仁周的宽圈,甚至复盖于整个核仁。碱性磷酸酶在核仁区的分布,在细胞刚分裂后布满于整个核仁,随后与DNA的分布一致,主要出现于核仁周的异染色貭区域。衰老培养、缺营养培养与Co~(60)射线影响后,细胞不进行分裂或分裂相大为减少,核仁周异染色貭亦减少。照射后的细胞继续培养,在出现大量分裂相前,核仁周异染色貭增加。以C~(14)-甘氨酸结合细胞,同位素自显术显示射迹颗粒,发现它们首先出现于核仁周,随着结合时间的增长,核仁内的颗粒增加,而且核仁区的颗粒数随着核仁周异染色貭的增多而增加。

 
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