Inoculate plant stem top in Culture Medium Ms + 6 - BA0. 5 - 1. 0mg/L + IAA0. 2mg/L, form grow thickly bud, cut open grow thickly bud. Inoculate it in MS + IAA0. 2mg/L Culture Medium form seeding, by check and measure, succeed in obtaining plant without virus.
All this showed that the isolate resembles the reported CMV.CMV-As was confirmed to belong to Cucumovirus by the biological properties detection, but because of the high similarity of the subgroup I and subgroup II, only the biological properties check can’t ensure the kind of CMV-As.
The non-pollutant vegetable production of Mu ye district in Xinxiang has gained great achievements in the recent years,but there are some problems such as low level,single breed,severe plant diseases and insect pests,lack of check,and weak process capacity.
Through the check by microscope, isolation, cultivation and observation of pathogens, determination of pathogenicity, and investigation of field disease, it was preliminarily identified as fusarium root rot disease of Pueraria thomsonii, and the pathogens was Fusarium solani, belonging to Deuteromycotina.
The check results of the optimum grade about the data fitting indicated that model output fitted well the trend of observed data,which verified that estimated parameters were credible,and model was valid on a certain scale.
Methods: Using the time series of cucumber downy mildew disease,we established an autoregressive integrated moving average model,ARIMA(2,2,0) based on model identification,comparison of residual variance,estimation and verification of parameter,observation of the correlation of the estimates matrix,autocorrelation check of the residuals,analysis of the fitting of model and so on.
Conclusion: Limiting the alternatives of model by residual variance, together with parameters estimation,the correlation of the estimates matrix,the autocorrelation check of the residuals and the fitting test,can help to search for suitable model quickly and accurately.
Average length of short fruit node is at 0.24 cm,0.23 cm,0.20 cm shorter than check,average length of middle fruit node is at 0.12 cm, 0.18 cm, 0.08 cm shorter than check if being treated by 8 g,10 g,12 g.
The result showed that coarse fibre content based on dry weight was significantly different among grasses,JV (from Jialin River Area) was the most,higher than check Jackpot,M28 and M22 (from Min River Area) were the next,Q10(from Qingyi River Area) was the least.