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vitro culture     
相关语句
  离体培养
     We have set up the following system of in vitro culture combined with γ ray irradiation to obtain mutant plants: (1) inducing callus from sweet potato stem explants cultured on the MS medium containing NAA 1mg/L,IAA 2mg/L and BA 0.01mg/L;
     建立了获得突变株的离体培养和辐射系统 :( 1)由甘薯茎在含有NAA 1mg/L ,IAA 2mg/L和BA 0 .0 1mg/L的MS培养基上诱导愈伤组织 ;
短句来源
     The primary study on Juglans nigra in vitro culture and propagation showed:6-BA 0.5-4 mg/L and NAA 0.01-0.5 mg/L all favourate sprouting and growing of axillary-bud or top-bud.
     对美国黑核桃离体培养及其繁殖技术进行了初步研究,结果表明:6-BA 0.5~4 mg/L和NAA 0.01~0.5 mg/L组合均能促进腋芽或顶芽的萌发与生长。
短句来源
     red prince were used as the explants to sceen the suitable media in vitro culture. The results indicated that MS or WPM as basic medium,added suitable proportion cytokinin(0.1-1mg·L-16-BA)and auxin(0.01-0.1mg·L-1NAA)was appropriate.
     结果表明 :红王子锦带的离体培养以MS、WPM为基本培养基 ,附加适当比例的细胞分裂素(0.1~1mg·L-16 -BA)和生长素(0.01~0.1mg·L-1NAA)为宜。
短句来源
     Stem sections of side autumn shoots of 'Thompson seedless' grape as in vitro plants adopted in middle September were cultured. The suitable culture medium B5+6-BA 2.0 mg/L+IBA0.5 mg/L+3% suger+0.6% agar,pH was regulated 5.8~6.0.It was the best medium for in vitro culture of grape stem section for bud initiation.
     该试验采用无核白鸡心葡萄秋季副梢的茎段为外植体,最佳的取材时间为9月中旬,适宜的基本培养基为B5+6-BA2.0mg/L+IBA0.5mg/L+3%蔗糖+0.6%琼脂,pH值调至5.8~6.0,为无核白鸡心葡萄茎段离体培养的最佳启动萌芽培养基;
短句来源
     By in vitro culture and adding 0.5 mol/L BA,1.0 mol/L BA+0.5 mol/L NAA to culture media, the seed dormancy can be broken after stored for three months in sand.
     将桂花种子进行3个月层积处理后,进行离体培养,培养基中加入0.5 mol/L BA,1.0 mol/L BA+0.5NAA可以打破种子的休眠,促进萌发。
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  体培养
     We have set up the following system of in vitro culture combined with γ ray irradiation to obtain mutant plants: (1) inducing callus from sweet potato stem explants cultured on the MS medium containing NAA 1mg/L,IAA 2mg/L and BA 0.01mg/L;
     建立了获得突变株的离体培养和辐射系统 :( 1)由甘薯茎在含有NAA 1mg/L ,IAA 2mg/L和BA 0 .0 1mg/L的MS培养基上诱导愈伤组织 ;
短句来源
     The primary study on Juglans nigra in vitro culture and propagation showed:6-BA 0.5-4 mg/L and NAA 0.01-0.5 mg/L all favourate sprouting and growing of axillary-bud or top-bud.
     对美国黑核桃离体培养及其繁殖技术进行了初步研究,结果表明:6-BA 0.5~4 mg/L和NAA 0.01~0.5 mg/L组合均能促进腋芽或顶芽的萌发与生长。
短句来源
     red prince were used as the explants to sceen the suitable media in vitro culture. The results indicated that MS or WPM as basic medium,added suitable proportion cytokinin(0.1-1mg·L-16-BA)and auxin(0.01-0.1mg·L-1NAA)was appropriate.
     结果表明 :红王子锦带的离体培养以MS、WPM为基本培养基 ,附加适当比例的细胞分裂素(0.1~1mg·L-16 -BA)和生长素(0.01~0.1mg·L-1NAA)为宜。
短句来源
     Stem sections of side autumn shoots of 'Thompson seedless' grape as in vitro plants adopted in middle September were cultured. The suitable culture medium B5+6-BA 2.0 mg/L+IBA0.5 mg/L+3% suger+0.6% agar,pH was regulated 5.8~6.0.It was the best medium for in vitro culture of grape stem section for bud initiation.
     该试验采用无核白鸡心葡萄秋季副梢的茎段为外植体,最佳的取材时间为9月中旬,适宜的基本培养基为B5+6-BA2.0mg/L+IBA0.5mg/L+3%蔗糖+0.6%琼脂,pH值调至5.8~6.0,为无核白鸡心葡萄茎段离体培养的最佳启动萌芽培养基;
短句来源
     By in vitro culture and adding 0.5 mol/L BA,1.0 mol/L BA+0.5 mol/L NAA to culture media, the seed dormancy can be broken after stored for three months in sand.
     将桂花种子进行3个月层积处理后,进行离体培养,培养基中加入0.5 mol/L BA,1.0 mol/L BA+0.5NAA可以打破种子的休眠,促进萌发。
短句来源
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  体外培养
     Study on in vitro culture of blastomeres derived from mouse 2,4,8-cell embryos
     小鼠2、4、8-细胞胚胎卵裂球体外培养的研究
短句来源
     It was found that (68.8 + 15.4) % of CD34 + cells expressed Flt3,(50.6+12.7)% of CD34+ cells expressed c-kit,the proportion of CD34 + cells expressing Flt3 and c-kit decreased as in vitro culture time extended.
     研究结果显示,新鲜脐血CD34~+细胞中(68.8±15.4)%为Flt3~+,(50.6±12.7)%为c-kit~+,随着体外培养时间的延长,二者表达逐渐下降。
短句来源
     Results The activation rate of 16-h and 19-h oocytes was high up to 63.6 % and 76.7 % respectively, but it was decreased and the lysed+dead rate increased obviously after in vitro culture, especially of 19-h oocytes.
     结果注射HCG后16、19h卵母细胞电激活率较高,分别为63.6%和76.7%,经体外培养后激活率下降,碎裂+死亡率明显增加。
短句来源
     At the 20 day in vitro culture, in both the test group 3 and the test group 4, the ratio of development follicles were 15.79% and 25.58%, and the maximum increase diameter of follicles were 300 μm and 320 μm, and small antrum of follicles came into being (the rates of antrum formation were 7.69 % and 17.65 %, respectively).
     体外培养第20 d,试验3 组和试验4 组卵泡发育率分别为15.79 %和25.58 %,卵泡最大增长直径分别为300 和320 μm,并形成小的卵泡腔(成腔率分别为7.69 %和17.65 %),而试验1 组和试验2 组其卵泡发育率均为0。
短句来源
     In vitro Culture of Umbilical Cord Blood MNC and CD34~+ Selected Cells
     体外培养脐血单个核细胞与CD34~+富集细胞
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  组织培养
     Studies on the process of shoot tip in vitro culture and plantlet regeneration of Liriope spicata var.prolifera showed that the process of induction propagation and differentiation of callus from the shoot tip was observed on the MS medium with 2.0 mg/L 6-BA and 0.2~0.5 mg/L NAA. The rooting of differentiated shoots and plantlet regeneration could be finished on the 1/2MS medium with 0.5 mg/L NAA.
     研究了湖北麦冬茎尖离体组织培养和植株再生过程 ,结果表明 :湖北麦冬茎尖在MS +6-BA 2 .0mg/L +NAA 0 .2~ 0 .5mg/L的培养基中能完成愈伤组织的诱导、增殖与分化 ,分化的小苗在 1 /2MS +NAA 0 .5mg/L的培养基中生根而完成植株再生过程。
短句来源
     In Vitro Culture and Polyploid Induction of Eucalyptus 12ABL
     刚果12号桉(Eucalyptus 12ABL)组织培养及多倍体诱导的研究
短句来源
     By using plant tissue culture technology and HPLC techniques, the biotransformation of 9 active chemicals by the 3 in vitro culture systems of Rheum palmatum was studied.
     利用植物组织培养并结合HPLC检测技术,对鬼臼毒素等9种活性化合物在掌叶大黄发状根、离体培养根及悬浮细胞3种培养系统中的生物转化进行了研究。
短句来源
     Effect of Ion Beam Irradiation on in vitro Culture of Cotton.
     离子注入对棉花离体组织培养影响初报
短句来源
     INDUCTION OF MUTANTS IN JUJUBE In vitro CULTURE BY ~(60)Coγ-RAY IRRADIATION
     酸枣 (Zizyphus jujuba Mill)组织培养结合~(60)Co- γ射线辐照诱发突变株(英文)
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      vitro culture
    Genetic Control of Totipotency of Plant Cells in an in vitro Culture
          
    The main approaches have been considered to studying the genetic control of plant cell totipotency in an in vitro culture.
          
    Morphogenesis of different Stachys species introduced in in vitro culture have been compared.
          
    The experiments conducted with in vitro culture of apical meristems showed that the observed changes were regulated on the local level.
          
    To develop a rabbit corneal endothelial (RCE) cell line, in vitro culture of RCE cells was initiated from Oryctolagus curiculus corneas and a novel RCE cell line was established in this study.
          
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    The present paper deals with in vitro culture of immature sunflower embryos. The basic medium consists of modified White's medium, supplemented with thiamine 1 ppm, pyridoxine 1 ppm, nicotinic acid 1 ppm, Ca-pantothenate 0.5 ppm and glycine 3 ppm. and 4% sucrose and 0.8% agar were used throughout all the experiments. IAA and coconut milk (autoclaved) at different concentrations used singly or both mixed in various combinations were studied for their effect on embryo growth. The embryos isolated were about...

    The present paper deals with in vitro culture of immature sunflower embryos. The basic medium consists of modified White's medium, supplemented with thiamine 1 ppm, pyridoxine 1 ppm, nicotinic acid 1 ppm, Ca-pantothenate 0.5 ppm and glycine 3 ppm. and 4% sucrose and 0.8% agar were used throughout all the experiments. IAA and coconut milk (autoclaved) at different concentrations used singly or both mixed in various combinations were studied for their effect on embryo growth. The embryos isolated were about 5 mm in length and they usually germinate precociously when inoculated on the culture medium. The results are summarized as follows: 1. IAA (0.05—20 ppm) is stimulatory on the embryo growth as shown in the increase of fresh weight of the treated embryos. IAA is effective in inducing embryo callus. At the concentration of 1 ppm there are about one fourth embryos producing callus, at 5 ppm, about two thirds of embryos, and above 10 ppm, 100% embryos forming callus. IAA is stimulatory on the formation and growth of the root at low concentrations (0.05—0.1 ppm), but it becomes inhibitory on the root growth at higher concentrations (10—20 ppm). 2. Coconut milk (autoclaved) has definitely a pronounced favourable effect on th shoot growth of the embryo and it inhibits the root growth. Both effects are particularly conspicuous at the stage of two weeks old culture, thereafter the difference between the control and the treated embryos gradually becomes insignificant. Coconut milk is also effective in inducing embryo callus but it is not so effective as IAA. 3. The effects on growth and induction of embryo callus become more pronounced when IAA and coconut milk are mixed in various combinations for treatment. There are certain similarities between the effect of coconut milk and that of IAA but it is improbable that the effective substance in coconut milk is only an auxin.

    五、摘要根据向日葵胚(约5毫米长)的离体培养试验,我们得出下列结论: 1.IAA(0.05—20ppm)对于生长有促进作用,表现在鮮重的显著增加。IAA有引起胚愈伤组织形成的作用,1ppm IAA约有1/4的胚长愈伤组织,5ppm的约2/3,10ppm以上的100%胚长愈伤组织。IAA在低浓度时(0.05—0.1ppm)对根的形成与生长有促进作用,在高浓度时(10—20ppm)对根的形成与生长有明显抑制作用。 2.椰子乳汁(经高压消毒)对苗的生长有显著促进作用,对于根的生长有抑制作用,这种现象在培养前两周差别特别明显,在后期差别逐渐缩小。高浓度的椰子乳汁(20%以上)也可以引起愈伤组织的形成,但不如IAA有效。 3.当IAA和椰子乳汁混合处理时,对于生长的影响及愈伤组织的形成表现得更为明显。而椰子汁与IAA的作用有某些类似之处,但不能表明椰子乳汁中起作用的物质只是一种IAA。

    The purpose of the present study was to select avirulent strains of leptospira pomona for preparative of live vaccine and to test their safety and efficacy in hamsters and pigs.Four strains ( L18. Dan-3. 7005. 7009) selected were averulent. They were able to make hamsters neither die nor become carrier state and were proved to be highly immunogenic. Amony them strain L18 was studied in detail.1.It was found that leptospires of strain Ll8 might spread to all organs of hamsters after they were inocucated and would...

    The purpose of the present study was to select avirulent strains of leptospira pomona for preparative of live vaccine and to test their safety and efficacy in hamsters and pigs.Four strains ( L18. Dan-3. 7005. 7009) selected were averulent. They were able to make hamsters neither die nor become carrier state and were proved to be highly immunogenic. Amony them strain L18 was studied in detail.1.It was found that leptospires of strain Ll8 might spread to all organs of hamsters after they were inocucated and would clean from the body in three days.The germs could not be transfered by successive passages through hamsters, but they could be transfered by alternative passages through hamsters and in vitro culture and no chang in virulence occurred after such passages for 10 times.2.An effective immunity in hamsters was produced after inoculation of the strain L18 live vaccine. It lasts at least nine months ( observed period) .3.Leptospires of strain L18 were unable to swine become renal carrier state either.70 pigs were alloted into two groups at random. 36 pigs were vaccinated with live leptospires of strain L18 and 34 pigs were not vaccinated as control. After challenge 33 pigs of the control group shed leptospires frequently in their urine, but only 2 of the vaccinated group shed leptospires occasionally.

    本文报告了四株(L18株、丹3株、7005株、7009株)钩端螺旋体弱毒株的毒力和免疫原性。实验结果证明,四株弱毒株均不造成地鼠发病和肾带菌,且有较好的免疫原性。其中L18株在地鼠体内只活存三天,不能经地鼠传代,通过地鼠—培基—地鼠连续传10代,未见其毒力增强。用L18株一次免疫(皮下、腹腔内、或肌内接种)地鼠有较好的免疫力,其保护力至少持续九个月(最长观察时间)。

    By using Simonsen's assay method and the techniques for the determinationof CFU-S with irradiated recipient mice and of CFU-C with in vitro culture me-thod,the characteristics of immunocompetent cells and of haemopoietic stem cellsseparated by velocity sedimentation from murine spleen cells and peripheral leu-cocytes were investigated.In the experiment of spleen cells the sedimentationvelocities of total nucleated cells,CFU-S,CFU-C,and the responsive cells to Simon-sen's assay method were 3.57,4.50,6.69...

    By using Simonsen's assay method and the techniques for the determinationof CFU-S with irradiated recipient mice and of CFU-C with in vitro culture me-thod,the characteristics of immunocompetent cells and of haemopoietic stem cellsseparated by velocity sedimentation from murine spleen cells and peripheral leu-cocytes were investigated.In the experiment of spleen cells the sedimentationvelocities of total nucleated cells,CFU-S,CFU-C,and the responsive cells to Simon-sen's assay method were 3.57,4.50,6.69 and 3.57 mm/hr respectively.In thecase of peripheral leucocyte the sedimentation velocities of total nucleated cells,CFU-S and the responsive cells to Simonsen's assay method were 4.49,4.96 and 5.12mm/hr respectively.The results showed that haemopoietic stem cells and im-munocompetent cells in the spleen are different in sedimentation velocities andprofiles,but such differences found in the peripheral leucocytes are less prominent.Among the haemopoietic stem cells the CFU-C cell volume is larger than thatof CFU-S,so that the separation of immunocompetent cells from CFU-C is mucheasier than from CFU-S.At present the separation of haemopoietic stem cellsfrom immunocompetent cells is a critical problem in the bone marrow transplan-tation.The prospect of using the principle of cell sedimentation for separatingimmunocompetent cells from haemopoietic stem cells,thereby reducing the severityof GVHR in haemopoietic stem cell transplantation is discussed.

    应用 Simonsen 的脾指数测定方法以及体内生成脾结节和体外琼脂培养方法,分别研究了小鼠脾脏和外周血中造血干细胞和免疫活性细胞在自然沉降条件下的沉降特性。在小鼠脾脏细胞的沉降试验中,CFU-S 的沉降速度为4.50毫米/小时,CFU-C 的沉降速度为6.69毫米/小时,脾指数阳性细胞的沉降速度为3.57毫米/小时。在小鼠外周血白细胞的沉降试验中,CFU-S 的沉降速度为4.96毫米/小时,脾指数阳性细胞的沉降速度为5.12毫米/小时。上述沉降试验表明,造血干细胞与免疫活性细胞有不同的沉降速度和分布特性,因此,有可能通过自然沉降,在一定程度上分开这两类细胞,其中,粒系定向造血干细胞(CFU-C)的沉降速度要高于多向性造血干细胞(CFU-S),因而,在沉降分离中 CFU-C 与免疫活性细胞可以得到较高程度的分离。从造血细胞中分离免疫活性 细胞是当前造血干细胞移植中的重要研究课题。本文对于应用速度沉降装置分离免疫活性细胞、减轻造血细胞移植中的移植物抗宿主反应(GVHR)的前景作了讨论。

     
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