Methods PCR was used to amplify HPV16E4 gene from cervical cancer tissue infected by HPV16.The plasmid,pET21b-HPV16E4,was then constructed by inserting the amplified HPV16 E4 gene into pET21b,a prokaryotic expression vector. PCR was used to screen the positive recombinant clones.
We concluded that serum sIL～2Rand CA125 alone might not be applied to identify and screen for ovarian cancer. Measurement of sll～2R combined with CA125 could significantly increase the specificity anddecrease false positive rate, therefore,it was helpful for the surgeon to draw up the furthertherapeutic program before operation and could raise the possibility of success.
Objective:To know the prevalence of bacterial vaginosis(BV)among pregnant women and to determine the risk factors related to BV.Methods:A cross-sectional study was conducted in a sample of 504 pregnant women in obstetric-genecology clinics to screen for BV.Non-conditional logistic regressions were used to identify the risk factors of BV.Results:The prevalence of BV among pregnant women was 18.1%.
We also demonstrate these spatial changes on the screen using Visual Basic language and GIS functions, which will help decision makers to have a clear view of species and site suitability changes over large areas.
The authors give a wave function of the state of particles on the screen in abovementioned configurations.
Many important proteins, such as some nucleoprotein transcriptional factor, carry out the regular method and construct the bait-BD vector to screen the library containing AD vector.
And we used this two-hybrid library to screen FOXA3, a hepatocyte nuclear factor, and found out an interacting protein: complement component C3.
Polymerase chain reaction and DNA sequencing were used to screen for KCNQ1, KCNH2, KCNE1, KCNE2 and SCN5A mutation.