Adjusting the pH of starch milk of sweet potato to 7. 5~8. 5 with saturated lime water and centrifuging it at 3,000-4,000 r/min. before precipitation seemed to be effective in raising the white - degree - date of sweet potato starch.
Two inactivated vaccine with different antigen concentration were prepared by using the supernatant materials obtained from centrifuging the ground emulsion of SPF whole embryos infected with AEV Van Rockel strain.
Controlling pH valule to the level of 6.0-7.0, adding 5g/L FeCl3? 6H2O, churnning up for 10 minutes, reposing for 30 minutes, and centrifuging under 2600r/min for 30 minutes, the concentrate of selenium in the castoff could accord with national standard.
(1)Seed protein extracting: The seeds are smashed and put into the 0.5ml tube, then adding 40 l water in the tube for forty minutes, then centrifuging 3 minutes at 10000 rpm, take out 15 l supernatant which will be diluted 2 times with regulation solution that has 10%TEMED, 15%urea and 30%HAC.
The result is following:the result before centrifuging of setting 60min in 37℃ (0.5ml tubule after-sperm motility 0.50 ,recovery percent 75.8%, 0.5ml pellet after-sperm motility 0.48 , recovery percent 72.5%, 0.1ml pellet after sperm motility 0.47,recovery percent 70.6%)was better than 30min and 90min(P<0.05);
The centrifuging method and enzyme treatment could not only (remove) the fat, lactose and the impurity in the liquid material, but also obviously improve the soluble viscosity, the color and the smell of the product.
Methods: RT PCR method was used to observe the expression of Leptin receptors (OB R) in male rat anterior pituitary. We used grade centrifuging method to get growth hormone (GH) cell, and [Ca 2+ ]i in GH cell was examined by laser scanning confocal system.