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binding
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  结合
    ABA-Binding Protein Encoding Gene Cloning, Expression in Yeast and Specific-ABA Binding of Its Product
    脱落酸(ABA)结合蛋白基因克隆、表达及其产物对ABA的特异结合
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    Identification of Critical Residues of GFRα1 Binding to GDNF
    GFRα1与GDNF结合位点的研究
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    Transcription Factor Binding Sites Prediction Algorithm Study and Application
    转录因子结合位点预测算法的研究与应用
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    Functional Analysis of the Genes Encoding RNA Binding Protein (Th-cpRBP29) and Na~+/K~+/Cl~--Cotransporter (Ls-Na~+/K~+/Cl~--Cotransporter)
    编码叶绿体/质体RNA结合蛋白及Na~+/K~+/Cl~-共转运体基因的功能研究
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    THE COOPERATIVE PHENOMENON IN THE BINDING OF LIGANDS TO OLIGOMERIC PROTEINS IN A TETRAHEDRAL MODEL
    四面体分子模型的寡聚蛋白结合配位体的协同现象
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  “binding”译为未确定词的双语例句
    Testosterone Influenced Expression of Notch Receptors and Ligand in Bone Marrow-derived Macrophages Through Binding to Surface Androgen Receptor
    睾酮通过细胞膜雄激素受体影响骨髓巨噬细胞中Notch受体及配体的表达
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    Preparation and Identification of Binding Sites of Monoclonal Antibodies Against SARS-CoV Spike Protein
    SARS病毒S蛋白特异性单克隆抗体制备和抗原识别位点鉴定
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    Screening and Preliminary Identification of Host Cellular Proteins Binding with Sars-CoV S and N Protein
    宿主细胞内SARS-CoV S蛋白和N蛋白的初步筛选和鉴定
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    Preliminary Study on 18KDa Binding Protein of Chlamydia Trachomatis
    沙眼衣原体18KDa粘连蛋白的初步研究
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    THE BINDING OF METHYL GREEN AND PYRONIN BY DNA
    甲基绿(Methyl green)及焦宁(Pyronin)与DNA的结合作用
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  binding
Allosteric Regulation of Binding and Function at GCPRS
      
cyclotriazadisulfonamides), viral envelope gp120-binding agents such as plant lectins and glycopeptide antibiotics, HIV integrase inhibitors such as the pyranodipyrimidine V-165, and two new classes of compounds (i.e.
      
3D-QSAR STUDIES ON SUBSTITUTED DIHYDROPYRIDINES FOR THEIR α1A-ADRENERGIC RECEPTOR BINDING AFFINITY
      
Both these models predicted binding affinity of internal and external test set compounds including the enantiomers of compound no.
      
All compounds were characterized by binding affinity determination for 5-HT2A and 5-HT2C subtypes and antagonistic activity for 5-HT2B receptor in rat stomach fundus.
      
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The papain catalyzed acyl-transfer reactions have been measured directly by the decrease inα-amino groups of the acceptor molecules. In 50% aqueous methanol, pH 7.4, papain catalyzed the acyl-transfer reactions between Cbz-Gly-NH_2, Cbz-Leu-NH_2 or Cbz-Ser-NH_2 as acyl donors and Gly-OEt as acyl acceptors; under theseconditions, the hydrolysis of the donor molecule did not take place. However, in 30% aqueous,acetone, the hydrolysis of the donor molecules was the only reaction detected. Reducing agents, such...

The papain catalyzed acyl-transfer reactions have been measured directly by the decrease inα-amino groups of the acceptor molecules. In 50% aqueous methanol, pH 7.4, papain catalyzed the acyl-transfer reactions between Cbz-Gly-NH_2, Cbz-Leu-NH_2 or Cbz-Ser-NH_2 as acyl donors and Gly-OEt as acyl acceptors; under theseconditions, the hydrolysis of the donor molecule did not take place. However, in 30% aqueous,acetone, the hydrolysis of the donor molecules was the only reaction detected. Reducing agents, such as cysteine, glutathione, cyanide, sulfite, [2, 3]-dimercaptopropanol orthioglycolic acid, stimulated both the hydrolytic and the acyl-transfer reactions catalyzed by papain.Unlike the other reducing agents, [2,3]-dimercaptopropanol or thioglycolic acid inhibited the acyl-transfer reactions at higher concentrations. The substrate specificities of the acyl-transfer and hydrolytic reactions have been compared.Under the conditions mentioned above, Cbz-Gly-NH_2, Cbz-Ser-NH_2 and Cbz-Leu-NH_2 but notBz-Arg-NH_2 or Cbz-Glu (NH_2)_2 served as donors for the acyl-transfer reactions. The estersand amides of a number of amino acids and peptides could serve as acyl acceptors. Among theamino acid esters tested, tyrosine ester did not take part in acyl-transfer reactions. A study of the effect of substrate concentrations on the rate of the acyl-transfer reaction withCbz-Gly-NH_2 as donor and Gly-OEt as acceptor showed that the acceptor was more tightly boundto the enzyme than the donor. Glycine ester also produced a lowering of the light absorption ofthe enzyme at 250 mμ and protected the enzyme against inactivation in aqueous methanol. Allthese pointed to a specific binding of the acceptor to the eozyme molecule. The significance of the above findings has been discussed.

本文提供以茚三酮法测受体α-氨基减少的方法以测定转肽反应。木瓜蛋白酶于50%甲醇,pH7.4左右条件下以CbZ-甘-NH_2,Cbz-白-NH_2及Cbz-丝-NH_2为供体,以甘-OEt为受体几乎只进行转肽作用而无供体本身的水解,但在30%丙酮溶液中却以水解反应为主。半胱氨酸、谷胱甘肽、氰化物、亚硫酸盐、[2,3]-二巯基丙醇及巯基乙酸皆可促进木瓜蛋白酶的转肽作用,但高浓度的[2,3]-二巯基丙醇及巯基乙酸与其他还原剂不同对转肽有抑制作用。转肽与水解的专一性不完全相同,如以Cbz-甘-NH_2,Cbz-丝-NH_2及Cbz-白-NH_2做供体时,几乎只进行转肽而无供体水解,但以Bz-精-NH_2及Cbz-谷NH_2-NH_2为供体时都无转肽作用。受体以氨基酸或肽的酯或酰胺为最好,酪氨酸乙酯不能做为转肽反应的受体。以Cbz-甘-NH_2,甘-OEt为供受体时底物浓度对反应速度的影响指出,受体与酶的结合远较供体紧密。甘-OEt的存在使木瓜蛋白酶于250mμ处光吸收有显著降低,并且对木瓜蛋白酶于甲醇溶液中的失活具有保护作用,说明受体与酶系专一性的结合。对本文实验结果的意义进行了讨论。

The inhibition of native aldolase by fructose-6-phosphate has been shown to be competitive. In sharp contrast, the residual activity of this enzyme towards fructose-1,6-diphosphate after digestion with carboxypeptidase was not inhibited by fructose-6-phosphate. On the other hand, the substrate, fructose-l,6-diphosphate, has been found to be capable of protecting aldolase from digestion by carboxypeptidase. It is therefore concluded that the C-terminal tyrosine residues, released during carboxypeptidase digestion,...

The inhibition of native aldolase by fructose-6-phosphate has been shown to be competitive. In sharp contrast, the residual activity of this enzyme towards fructose-1,6-diphosphate after digestion with carboxypeptidase was not inhibited by fructose-6-phosphate. On the other hand, the substrate, fructose-l,6-diphosphate, has been found to be capable of protecting aldolase from digestion by carboxypeptidase. It is therefore concluded that the C-terminal tyrosine residues, released during carboxypeptidase digestion, are involved in the binding of the substrate, fructose-l,6-diphosphate, and the competitive inhibitor, fructose-6-phosphate, to the enzyme.Treatment of the data given by Drechsler et al. by the method of Tsou shows that two of the three C-terminal tyrosine residues are essential for the activity of aldolase.

醛縮酶經羧肽酶消化催化底物果糖-1,6-二磷酸分解的活力下降至7%以后,竞爭性抑制物果糖-6-磷酸对酶不再有抑制作用,說明被羧肽酶消化切除的C末端酪氨酸和酶与竞爭性抑制物的結合有关。此外还发现底物果糖-1,6-二磷酸能保护醛縮酶不受羧肽酶消化。因此我們认为C末端酪氨酸是醛縮酶的活性中心。酶分子通过它和竞爭性抑制物果糖-6-磷酸及底物果糖-1,6-二磷酸的-6-磷酸部分結合。利用邹承魯新近提出的方法推算,醛縮酶的三个酪氨酸末端中有二酪氨酸是酶的活性中心。

A suspension of penicillin spheroplasts of E. colt K-12 were treated with the detergent Lissapol(2.5% v/v). The lysate obtained was centrifuged at 30,000 g for 20 minutes and the pellet after repeated washings employed for experimentations.Glycine incorporation was stimulated by the addition of the 2',3'-nucleotides or nucleosides of uracil, adenine, guanine, and cytosine, among them uridylic acid being usually the most active one. Ribose was inactive and the free purine and pyrimidine bases were even slightly...

A suspension of penicillin spheroplasts of E. colt K-12 were treated with the detergent Lissapol(2.5% v/v). The lysate obtained was centrifuged at 30,000 g for 20 minutes and the pellet after repeated washings employed for experimentations.Glycine incorporation was stimulated by the addition of the 2',3'-nucleotides or nucleosides of uracil, adenine, guanine, and cytosine, among them uridylic acid being usually the most active one. Ribose was inactive and the free purine and pyrimidine bases were even slightly inhibitory.The increase in incorporation rate caused by a mixture of the four 2',3'-nucleotides was not greater than that produced by the most active one namely uridylic acid.Ribonucleic acid, either soluble or high molecular one, accelerated glycine incorporation. However, this effect was not observed consistently and varied with different batches of the spheroplast preparation.A comparatively minor fraction of the incorporated glycine was found in either the C-or the N-terminals of the proteins isolated from the spheroplasts, thus indicating that the amino acid was bound inside the protein molecules. The same result was noted when the incorporation rate was stimulated by the addition of 2',3'-uridylic acid.

用Lissapol处理的大腸杆菌原生貭体的制剂具有C~(14)-甘氨酸的参入活性,RNA有提高参入的能力,但不稳定;碱水解的RNA則有更显著的作用。四种2′,3′-核苷酸对甘氨酸参入都有刺激,一般以尿嘧啶核苷酸的效应为高,核苷的刺激作用較核苷酸为差,核糖无影响,碱基稍有抑制,用羧肽酶水解及2,4-二硝基氟苯处理标記的蛋白貭,証明C~(14)-甘氨酸参入到蛋白貭的肽鏈中。

 
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