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   p 16 mrna 的翻译结果: 查询用时:0.01秒
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p mrna     
相关语句
  p16基因mrna
     Expression of p16 mRNA and p16 Protein in Gastric Carcinoma and its Clinico pathological Significance
     胃癌组织中p16基因mRNA和p16蛋白的表达及临床病理意义
短句来源
     ② The p16 mRNA transcription levels of R 20, R 21, T 35 and T 54 cells were much lower than that of normal BEP2D cell.
     ②与正常BEP2D细胞相比 ,R 2 0、R 2 1、T 3 5和T 5 4细胞 p16基因mRNA转录水平均降低。
短句来源
     Both the level of p16 mRNA and its product are correlated with lymph nodes metastasis and patient survival ( P <0 05).
     p16基因mRNA及其蛋白表达水平与乳腺癌的腋窝淋巴结转移呈显著负相关 (P <0 0 5 ) ,而与乳腺癌的术后生存期呈显著正相关 (P <0 0 5 ) ;
短句来源
     Detectable expression of both p16 mRNA and P16 protein was fou nd in the cells treated with 1.0 靘ol/L and 2.0 靘ol/L As2O3. (3)Expression le vels of DNMT 3A and 3B mRNA were increased in the treated cells and were depende nt on As2O3 concentration, however no significant difference was found between e ach two groups (P >0.05).
     1.0μmol/L和2.0μmol/LAs2O3处理的U266细胞还扩增出p16基因mRNA产物;
短句来源
     Results: Only CAOV3 cells of 5 ovarian cancer cell lines had homozygous deletion of p16. Neither p16 mRNA nor p16 protein was detected in CAOV3 cells.
     结果:5 种人卵巢上皮癌细胞系中只有CAOV3(20% ) 显示p16 基因纯合性缺失,CAOV3 细胞亦无p16 基因m RNA 及蛋白表达。
短句来源
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  p16基因
     Expression of p16 mRNA and p16 Protein in Gastric Carcinoma and its Clinico pathological Significance
     胃癌组织中p16基因mRNA和p16蛋白的表达及临床病理意义
短句来源
     5. Relationship of p16 gene structure,p16mRNA and p16 protein (1) p16 mRNA in 14 patients with p16 mythelation were not dectable.
     5.P16基因结构与其表达综合分析:(1)14例pl6基因甲基化的实验组样本中,相应的P16mRNA检测均为阴性。
短句来源
     ② The p16 mRNA transcription levels of R 20, R 21, T 35 and T 54 cells were much lower than that of normal BEP2D cell.
     ②与正常BEP2D细胞相比 ,R 2 0、R 2 1、T 3 5和T 5 4细胞 p16基因mRNA转录水平均降低。
短句来源
     Conclusions: 8 Br cAMP and quercetin could induce DBP bound to p16 gene promoter to up regulate p16 mRNA expression.
     结论 :8 Br cAMP和quercetin尤其是前者作用于人食管癌Eca 10 9细胞 ,诱导产生与p16基因启动子结合较强的DBP ,启动p16基因表达
短句来源
     The methylation level of p16 gene was gradually decreased and the p16 mRNA expression restored after exposure to 5 aza CdR.
     用 5 aza CdR后 ,SL I的p16基因甲基化水平逐渐降低 ,mRNA表达逐渐恢复。
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  p16基因转录
     Changes in p16 mRNA level and protein expression in thymocytes and splenocytes after whole body irradiation
     整体照射后胸腺细胞和脾细胞p16基因转录和蛋白表达的变化
短句来源
     Northern blot was applied to determine p16mRNA level.
     采用Northern blot检测p16基因转录水平的变化;
短句来源
     To investigate the effect of ionizing radiation on p16 gene transcription and protein expression in EL-4 cells in vitro, RT-PCR semi-quantity method was applied to determine the p16mRNA level and immunocytochemistry (ICC) was employed to measure the p16 protein expression.
     PCR(逆转录聚合酶链反应)半定量方法检测p16基因转录水平的变化,采用免疫细胞化学方法检测蛋白表达,观察X射线照射对离体培养的EL? 4细胞p16基因转录及蛋白表达的影响,证实p16基因转录表达在辐射诱导EL?
短句来源
  抑癌基因p16mrna
     The Clinical Significance of Expression of p16 mRNA in Cancer of Urinary Bladder
     抑癌基因p16 mRNA表达在膀胱癌中的意义
短句来源

 

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      p mrna
    Low CDKN2/p16 mRNA expression had the same topographic distribution as nuclear CDKN2/p16 immunoreactivity proving for reliability of the immunocytochemical findings.
          
    The surviving fraction of the p16-transfected A549p16 and H460p16 cells that expressed exogenous p16 mRNA or protein was lower than those of the parental and negative control cells.
          
    P16 mRNA in 2-, 3-, 4-, 5-day CM group were 1.63 times (p>amp;lt;0.01), 1.72 times (p>amp;lt;0.01), 1.99 times (p>amp;lt;0.01) and 2.84 times (p>amp;lt;0.01) of that in 1-day group respectively.
          
    The p16 mRNA and protein were not detectable in the remaining OS cell lines that contained high levels of Rb protein.
          
    This p16 mRNA, however, remained expressed in this cell line.
          
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    The expression of glutathione S transferase placental (GST P) mRNA was observed in hepatic preneoplastic lesions induced with diethylnitrosamine (DEN) with in situ hybridization in rats. It was found that GST P mRNA mainly expressed in altered foci and some of oval cells in hepatic preneoplastic lesions and the extent of its expression was different among foci or/and positive cells in the same foci whereas no expression was observed in normal and regenerating hepatic tissue. Our findings...

    The expression of glutathione S transferase placental (GST P) mRNA was observed in hepatic preneoplastic lesions induced with diethylnitrosamine (DEN) with in situ hybridization in rats. It was found that GST P mRNA mainly expressed in altered foci and some of oval cells in hepatic preneoplastic lesions and the extent of its expression was different among foci or/and positive cells in the same foci whereas no expression was observed in normal and regenerating hepatic tissue. Our findings indicate that cells in altered foci and oval cells may be preneoplastic cells in the experimental hepatocellular carcinoma at the molecular level.

    应用原位杂交技术,观察了二乙基亚硝胺(DEN)诱发大鼠肝癌前病变组织中胎盘型谷胱甘肽S转移酶(GST-P)mRNA的表达。结果显示,GST-PmRNA主要在癌前病变肝组织中的变异灶及灶外卵圆形细胞内表达,且在变异灶间或和同一灶内阳性细胞间表达程度不尽一致,而正常肝、再生肝组织中未见其表达。提示在分子水平上变异灶细胞及卵圆型细胞可能成为实验性肝癌的癌前期细胞

    Digoxigenin-labelled P53and c-myc cDNA probes were used to study gallbladder carcinoma by in situhybridization on the paraffin sections for mRNA expression in 21 cases.The resillts were as follows:c-myc and P53mRNAexpressiOn were both located in the nuclei.The positive incidences of expression of both c-myc and P53mRNA in gallbladderwere 66.6%and 5 7.1%respectivelyNo expression in the normal tissue surrounding the carcinoma.The strength of theexpression of c-myc and P53mRNA...

    Digoxigenin-labelled P53and c-myc cDNA probes were used to study gallbladder carcinoma by in situhybridization on the paraffin sections for mRNA expression in 21 cases.The resillts were as follows:c-myc and P53mRNAexpressiOn were both located in the nuclei.The positive incidences of expression of both c-myc and P53mRNA in gallbladderwere 66.6%and 5 7.1%respectivelyNo expression in the normal tissue surrounding the carcinoma.The strength of theexpression of c-myc and P53mRNA in gallbladder were related to the size of primary tumors,metastasis of lymph node anddistance metastasis,However,there was no relationship between the expression of p"mRNA and the degree of carcinoma celldifferentiation but c-myc mRNA.

    应用地高辛标记的P ̄(53)、c-myccDNA探针对21例胆囊癌石腊切片上的mRNA进行原泣杂交。结果表明c-myc和P ̄(53)mRNA的表达均位于细胞核内。c-myc和P ̄(53)mRNA在胆囊癌的表达率分别为66.6%和57.1%,在癌周正常组织中没有c-myc、P ̄(53)mRNA的表达。c-myc、P ̄(53)mRNA表达的强度和胆囊癌的原发肿瘤大小,有无淋巴结转移及远处转移有关。分期越晚,表达率越高。有淋巴结转移者高于无转移者。P ̄(53)mRNA表达的阳性率和胆囊癌的分化程度无关;而c-mycmRNA的表达和分化程度有关,分化越差,表达率越高。

    CD34 + celis contain hemopoietic stem progenitor celis, vvhich can be induced to differentiate and proli-ferate by interleukine-3 (IL-3), granulocvte-macrophage colonj'-stimulating factor (GM-CSF) and granulocyte-CSF (G-CSF) etc. Whether normai CD34+ celis participate in the production of any cytokines remains uncertain. We inves-tigated IL-lp, IL-3, IL-6, IL-4, GM-CSF, TNF-a, TNF-P and c-kit gene expression by using reverse transcription-polvmerase chain reaction {RT-PCR) in flow cvtometric sorted normai...

    CD34 + celis contain hemopoietic stem progenitor celis, vvhich can be induced to differentiate and proli-ferate by interleukine-3 (IL-3), granulocvte-macrophage colonj'-stimulating factor (GM-CSF) and granulocyte-CSF (G-CSF) etc. Whether normai CD34+ celis participate in the production of any cytokines remains uncertain. We inves-tigated IL-lp, IL-3, IL-6, IL-4, GM-CSF, TNF-a, TNF-P and c-kit gene expression by using reverse transcription-polvmerase chain reaction {RT-PCR) in flow cvtometric sorted normai bone marrow CD34 + celis. We also analyzed the effects of recombinant IL-1P, IL-3, IL-7, GM-CSF, PIXY-321 (fusion protein IL-3/GM-CSF) and stem celi factor (SCF) on the regulation of these cvtokines. It was found that freshly sorted CD34 + celis have high Ievels of IL-1P, c-kit mRNA and low Ievels of IL-3, TNF-a and TNF-P mRNA. After stimnlating with exogenous cvtokines, the Ievels of IL-1P, TNF-a and TNF-p mRNA were upregulated by all stimuli to various extent. GM-CSF mRNA became positive only under the effect of IL-7 stimulation. IL-7 also significantly enhanced IL-6 gene expression. None of these factors have a significant effect on c-kit or IL-3 transcription.

    CD34~+细胞包含有造血干/祖细胞,而造血干/祖细胞能被IL-3,GM-SCF,G-CSF等诱导分化与增殖。正常的CD34~+细胞是否参与一些细胞因子的产生尚未被阐明。我们采用逆转录-聚合酶链反应分析了经流式细胞仪分选出的正常骨髓CD34~+细胞IL-1β,IL-3,IL-4,IL-6,GM-CSF,TNF-α,TNF-β及c-kit基因的表达,并且分析了重组IL-1β,IL-3,IL-7,GM-CSF,PIXY321(IL-3/GM-CSF融合蛋白)及干细胞因子(SCF)对这些细胞因子的调节作用。结果发现新分选出的CD34~+细胞表达较高水平的IL-1β,c-kit mRNA及低水平的IL-3,TNF-α,TNF-βmRNA。经外源性的细胞因子刺激后,IL-1β,TNF-α及TNF-βmRNA均有不同程度的上调,唯IL-7能使GM-CSF mRNA的表达变为阳性,IL-7亦能显著增强IL-6基因的表达。所有这些细胞因子对c-kit及IL-3基因表达均无明显作用。

     
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